Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS306B10f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 91 7e-20
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 91 9e-20
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 83 3e-17
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 56 4e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 56 4e-09
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.42
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 1.3
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.3
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 3.0
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 6.8
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 25 9.0
SPAC30.02c |||RNA polymerase II elongator complex subunit|Schizo... 25 9.0
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 91.5 bits (217), Expect = 7e-20
Identities = 47/116 (40%), Positives = 72/116 (62%)
Frame = +2
Query: 158 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 337
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 338 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG 505
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKG 121
Score = 27.5 bits (58), Expect = 1.3
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 155 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 268
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 91.1 bits (216), Expect = 9e-20
Identities = 47/124 (37%), Positives = 76/124 (61%), Gaps = 2/124 (1%)
Frame = +2
Query: 137 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 310
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 311 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 490
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 491 QLLQ 502
+++
Sbjct: 125 DMIR 128
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 82.6 bits (195), Expect = 3e-17
Identities = 44/122 (36%), Positives = 74/122 (60%)
Frame = +2
Query: 137 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 316
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 317 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 496
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 497 LQ 502
+Q
Sbjct: 120 VQ 121
Score = 35.5 bits (78), Expect = 0.005
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +2
Query: 155 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 334
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 335 QAISK 349
+ ++K
Sbjct: 139 RIMAK 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 55.6 bits (128), Expect = 4e-09
Identities = 36/125 (28%), Positives = 59/125 (47%)
Frame = +2
Query: 128 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 307
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 308 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 487
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 488 EQLLQ 502
E +L+
Sbjct: 156 ESILR 160
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 55.6 bits (128), Expect = 4e-09
Identities = 37/124 (29%), Positives = 57/124 (45%), Gaps = 2/124 (1%)
Frame = +2
Query: 137 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 310
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 311 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 490
E F+ + K D + FD D G IS LR + L E + D E+E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 491 QLLQ 502
+++
Sbjct: 150 AMIE 153
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.42
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -2
Query: 490 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 320
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 1.3
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +2
Query: 314 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 481
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 482 EVEQLL 499
+E +L
Sbjct: 702 LLEHIL 707
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 1.3
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +2
Query: 155 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 334
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 335 QAIS 346
IS
Sbjct: 73 IHIS 76
Score = 26.2 bits (55), Expect = 3.0
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +2
Query: 398 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL 499
FD G+I +LR + LGE L+ ++++ +L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 3.0
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -1
Query: 236 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 63
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 62 SKSN 51
K+N
Sbjct: 276 IKAN 279
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 6.8
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 415 WVHLFCGTATPALY 456
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 727
Score = 24.6 bits (51), Expect = 9.0
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = +2
Query: 209 VAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTAN 373
+ Q+ D LR G + + + T + ERI+ E ++A+ KA GD +
Sbjct: 23 IPQLKDILRVFGLRLSGTKAELITRIKQLIERIAIENNTTSWEALKKAIDGDVTS 77
>SPAC30.02c |||RNA polymerase II elongator complex
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 281
Score = 24.6 bits (51), Expect = 9.0
Identities = 17/67 (25%), Positives = 31/67 (46%), Gaps = 4/67 (5%)
Frame = +3
Query: 171 SSSSILVEMERYMSLRSEMR*E-L*DKILQSQM*KNVLFI---LNLMKGYLLRCFCQFTR 338
+ S+ +E E Y ++E L +Q ++ K+ I LN +KG+ + +C+
Sbjct: 45 NDESLNIEKETYRESKNEKAARGLLYSAVQRELSKSTFVICDALNYIKGFRYQLYCESKS 104
Query: 339 PYRKHAV 359
Y H V
Sbjct: 105 MYTTHCV 111
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,043,753
Number of Sequences: 5004
Number of extensions: 41418
Number of successful extensions: 174
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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