Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS306A10f
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g62870.1 68416.m07063 60S ribosomal protein L7A (RPL7aB) 60S ... 120 7e-28
At2g47610.1 68415.m05940 60S ribosomal protein L7A (RPL7aA) 119 9e-28
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 31 0.52
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 29 1.2
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 29 1.2
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 29 1.2
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 29 1.2
At5g63950.1 68418.m08030 SNF2 domain-containing protein / helica... 29 2.1
At2g34240.1 68415.m04189 hypothetical protein contains Pfam prof... 29 2.1
At5g15680.1 68418.m01834 expressed protein 28 2.8
At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family pro... 27 6.4
At1g20530.1 68414.m02558 hypothetical protein 27 6.4
At5g11100.1 68418.m01296 C2 domain-containing protein similar to... 27 8.5
>At3g62870.1 68416.m07063 60S ribosomal protein L7A (RPL7aB) 60S
RIBOSOMAL PROTEIN L7A - Oryza sativa,
SWISSPROT:RL7A_ORYSA
Length = 256
Score = 120 bits (288), Expect = 7e-28
Identities = 52/83 (62%), Positives = 66/83 (79%)
Frame = +2
Query: 158 NPLFEKRPKNFAIGQGIQPTRDLSRFVRWPKYIRIQRQKAVLQRRLKVPPXINQFTQTLD 337
NPLFE+RPK F IG + P +DLSR+++WPK IR+QRQK +L++RLKVPP +NQFT+TLD
Sbjct: 20 NPLFERRPKQFGIGGALPPKKDLSRYIKWPKSIRLQRQKRILKQRLKVPPALNQFTKTLD 79
Query: 338 KTTAKGLFKILEKYRPETEQQKE 406
K A LFKIL KYRPE + K+
Sbjct: 80 KNLATSLFKILLKYRPEDKAAKK 102
>At2g47610.1 68415.m05940 60S ribosomal protein L7A (RPL7aA)
Length = 257
Score = 119 bits (287), Expect = 9e-28
Identities = 51/83 (61%), Positives = 66/83 (79%)
Frame = +2
Query: 158 NPLFEKRPKNFAIGQGIQPTRDLSRFVRWPKYIRIQRQKAVLQRRLKVPPXINQFTQTLD 337
NPLFE+RPK F IG + P +DLSR+++WPK IR+QRQK +L++RLKVPP +NQFT+TLD
Sbjct: 21 NPLFERRPKQFGIGGALPPKKDLSRYIKWPKSIRLQRQKRILKQRLKVPPALNQFTKTLD 80
Query: 338 KTTAKGLFKILEKYRPETEQQKE 406
K A LFK+L KYRPE + K+
Sbjct: 81 KNLATSLFKVLLKYRPEDKAAKK 103
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3)
{Arabidopsis thaliana}
Length = 1473
Score = 30.7 bits (66), Expect = 0.52
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +2
Query: 293 LKVPPXINQFTQTLDKTTAKGLFKILEKY-RPETEQQKES 409
L+VP + +F + K T KG K+L Y PE E+ KES
Sbjct: 562 LQVPSFLVEFITPIVKATRKGTKKVLSFYSMPEYEEWKES 601
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.5 bits (63), Expect = 1.2
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 426 VQQLPQLSFCCSVSGLYFSKILKRPLAVVLSSVWVNWLI 310
V Q PQL C V L+ ++ P+A+++ W +WLI
Sbjct: 52 VLQGPQLDILCGVLSLFAWIVVVSPIAILIG--WGSWLI 88
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.5 bits (63), Expect = 1.2
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 426 VQQLPQLSFCCSVSGLYFSKILKRPLAVVLSSVWVNWLI 310
V Q PQL C V L+ ++ P+A+++ W +WLI
Sbjct: 52 VLQGPQLDILCGVLSLFAWIVVVSPIAILIG--WGSWLI 88
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.5 bits (63), Expect = 1.2
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 426 VQQLPQLSFCCSVSGLYFSKILKRPLAVVLSSVWVNWLI 310
V Q PQL C V L+ ++ P+A+++ W +WLI
Sbjct: 52 VLQGPQLDILCGVLSLFAWIVVVSPIAILIG--WGSWLI 88
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 29.5 bits (63), Expect = 1.2
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 426 VQQLPQLSFCCSVSGLYFSKILKRPLAVVLSSVWVNWLI 310
V Q PQL C V L+ ++ P+A+++ W +WLI
Sbjct: 52 VLQGPQLDILCGVLSLFAWIVVVSPIAILIG--WGSWLI 88
>At5g63950.1 68418.m08030 SNF2 domain-containing protein / helicase
domain-containing protein low similarity to SP|Q03468
Excision repair protein ERCC-6 (Cockayne syndrome
protein CSB) {Homo sapiens}; contains PFam profiles
PF00271: Helicase conserved C-terminal domain, PF00176:
SNF2 family N-terminal domain
Length = 1090
Score = 28.7 bits (61), Expect = 2.1
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = -2
Query: 408 LSFCCSVSGLYFSKILKRPLAVVLSSVWVNWL 313
+ C ++GL+ SK++KR L V ++ +W+
Sbjct: 410 MQICSFLAGLFHSKLIKRALVVAPKTLLPHWM 441
>At2g34240.1 68415.m04189 hypothetical protein contains Pfam
profiles PF04780: Protein of unknown function (DUF629),
PF04781: Protein of unknown function (DUF627)
Length = 712
Score = 28.7 bits (61), Expect = 2.1
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +1
Query: 160 SSLREEAKELCHWSGHSANSGLVQICKMAQVYPHPAPEG 276
S+L + + LC H ++G V+ K + +Y H PEG
Sbjct: 451 SNLLLDGRLLCEEEHHFDDTGSVETFKSSGIYEHVIPEG 489
>At5g15680.1 68418.m01834 expressed protein
Length = 2151
Score = 28.3 bits (60), Expect = 2.8
Identities = 21/68 (30%), Positives = 28/68 (41%), Gaps = 3/68 (4%)
Frame = +1
Query: 118 PIGGQKG*AQEDRESSLRE---EAKELCHWSGHSANSGLVQICKMAQVYPHPAPEGCTSA 288
P+ G G ++SS E + K L + A SG +Q MA P P G +A
Sbjct: 2048 PVQGGTG-GSHPQDSSYSENGTDEKTLVPQTSFKARSGPLQYAMMAATMSQPFPLGAAAA 2106
Query: 289 SSESAPXD 312
S P D
Sbjct: 2107 ESGIPPRD 2114
>At2g19930.1 68415.m02329 RNA-dependent RNA polymerase family
protein contains Pfam domain, PF05183: RNA dependent RNA
polymerase
Length = 977
Score = 27.1 bits (57), Expect = 6.4
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 266 RQKAVLQRRLKVPPXINQFTQTLDKTTAKGLFKILEKYRPETEQQKES 409
R +++ + + PP + QF D KG F + +K P T Q + S
Sbjct: 424 RSESIQEACYQDPPLLIQFRMFYDGYAVKGTFLLNKKLCPRTVQVRPS 471
>At1g20530.1 68414.m02558 hypothetical protein
Length = 614
Score = 27.1 bits (57), Expect = 6.4
Identities = 12/53 (22%), Positives = 28/53 (52%)
Frame = +2
Query: 248 KYIRIQRQKAVLQRRLKVPPXINQFTQTLDKTTAKGLFKILEKYRPETEQQKE 406
K + +++++ +QR++K P + K+ + +FK +EK ++Q E
Sbjct: 555 KLMMLEKEEQKMQRKMKTVPSVALMGSLNLKSNMEQIFKSMEKLATNSKQTYE 607
>At5g11100.1 68418.m01296 C2 domain-containing protein similar to
Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
from [Lycopersicon esculentum]
Length = 574
Score = 26.6 bits (56), Expect = 8.5
Identities = 14/37 (37%), Positives = 23/37 (62%)
Frame = -1
Query: 235 KSGQVPSWLNALTNGKVLWPLLEERIHDLLGLNLFDH 125
K+ VP LN + N + ++E+ +HDLL L ++DH
Sbjct: 485 KTRVVPDSLNPVWNQTFDF-VVEDALHDLLTLEVWDH 520
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,084,536
Number of Sequences: 28952
Number of extensions: 172555
Number of successful extensions: 504
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 504
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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