Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS305G03f
(502 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ... 105 1e-23
At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase f... 44 4e-05
At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase f... 41 4e-04
At1g65010.1 68414.m07368 expressed protein similar to endosome-a... 31 0.33
At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family pro... 28 4.1
At3g59780.1 68416.m06671 expressed protein 27 7.1
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 27 7.1
At5g49970.2 68418.m06187 pyridoxamine 5'-phosphate oxidase-relat... 27 9.4
At5g49970.1 68418.m06188 pyridoxamine 5'-phosphate oxidase-relat... 27 9.4
At5g01400.1 68418.m00053 expressed protein contains low similari... 27 9.4
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 27 9.4
>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
putative / ubiquitin thiolesterase, putative similar to
SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
{Mus musculus}; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 234
Score = 105 bits (253), Expect = 1e-23
Identities = 52/111 (46%), Positives = 75/111 (67%), Gaps = 1/111 (0%)
Frame = +3
Query: 117 VPLESNPDVLNKFLQKLGV-PNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENH 293
+PLESNPDV+N++L LG+ P++ DV GLD E L VP+PVL+V+ L+PI+ E
Sbjct: 14 LPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLAVLFLYPITKKSEEE 73
Query: 294 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG 446
+ ++ EI K S +++MKQ + NACGTI L+H++ N T I+LSDG
Sbjct: 74 RIEQDKEIKEKVH--SDKVYFMKQTVGNACGTIGLLHAIGNITSEIKLSDG 122
>At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase
family 1 protein similar to 26S proteasome regulatory
complex subunit p37A [Drosophila melanogaster]
GI:6434962; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 334
Score = 44.4 bits (100), Expect = 4e-05
Identities = 37/127 (29%), Positives = 68/127 (53%), Gaps = 5/127 (3%)
Frame = +3
Query: 117 VPLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHK 296
+P+ES+P + + +Q++ V + ++ LD +L + RPV ++LL Y+
Sbjct: 4 LPVESDPGIFTEIIQQMQVKGV-QVEELYSLDFNSLDEI-RPVYGLILL------YKWRP 55
Query: 297 KTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDI---IELSD--GHMQKF 461
+ +EN ++ E + N F+ Q I+NAC T A++ + N++ I ELS+ ++F
Sbjct: 56 EEKENRVVIT--EPNPNFFFASQIINNACATQAILSVLMNSSSIDIGSELSELKQFAKEF 113
Query: 462 LNEAKGL 482
E KGL
Sbjct: 114 PPELKGL 120
>At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase
family 1 protein similar to 26S proteasome regulatory
complex subunit p37A [Drosophila melanogaster]
GI:6434962; contains Pfam profile PF01088: Ubiquitin
carboxyl-terminal hydrolase, family 1
Length = 330
Score = 41.1 bits (92), Expect = 4e-04
Identities = 25/102 (24%), Positives = 56/102 (54%)
Frame = +3
Query: 123 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 302
+ES+P V + +Q++ V + ++ LD ++L+ + RPV ++ LF ++ + T
Sbjct: 6 IESDPGVFTELIQQMQVKGV-QVEELYSLDSDSLNNL-RPVYGLIFLFKWQAGEKDERPT 63
Query: 303 EENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDI 428
++++ N+F+ Q I+NAC T A++ + N+ ++
Sbjct: 64 IQDQV--------SNLFFANQVINNACATQAILAILLNSPEV 97
>At1g65010.1 68414.m07368 expressed protein similar to
endosome-associated protein (GI:1016368) [Homo sapiens];
similar to Centromeric protein E (CENP-E protein)
(Swiss-Prot:Q02224) [Homo sapiens]
Length = 1318
Score = 31.5 bits (68), Expect = 0.33
Identities = 21/61 (34%), Positives = 33/61 (54%)
Frame = +3
Query: 294 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEA 473
KK+EE E S +EVS + +K++ +AC S+ NN + E ++Q+ L EA
Sbjct: 522 KKSEE-ENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEA 580
Query: 474 K 476
K
Sbjct: 581 K 581
>At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family
protein contains Pfam domain, PF05183: RNA dependent RNA
polymerase
Length = 992
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/55 (23%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = -1
Query: 265 KSSITESTGRGTHESVSGSSPMTSTIFHLFGTPSFCKNLFRT-SGLDSRGTKVSV 104
K + E+TG H+ + + +T + G ++C +L+ T G+ G V +
Sbjct: 229 KGHLLENTGTHLHKVLGDDNVLTVKFDKVLGVETYCNDLYSTYKGIAKNGIMVGL 283
>At3g59780.1 68416.m06671 expressed protein
Length = 610
Score = 27.1 bits (57), Expect = 7.1
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = -2
Query: 144 GHRDWIQEGLKFRSP 100
G+R W+QEGL+ + P
Sbjct: 440 GYRSWVQEGLRVKEP 454
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 27.1 bits (57), Expect = 7.1
Identities = 14/62 (22%), Positives = 28/62 (45%)
Frame = +3
Query: 135 PDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKTEENE 314
PD+L K +++ G +W V+ P L+ V + ++ FP + + + E
Sbjct: 82 PDLLGKTIEERGQVEQWLDVEATSYHPPLLALTLNIVFAPLMGFPADEKVIKESEEKLAE 141
Query: 315 IL 320
+L
Sbjct: 142 VL 143
>At5g49970.2 68418.m06187 pyridoxamine 5'-phosphate oxidase-related
contains weak similarity to Pyridoxamine 5'-phosphate
oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx).
(Swiss-Prot:P28225) [Shigella flexneri]
Length = 466
Score = 26.6 bits (56), Expect = 9.4
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 493 AVASSPLASFKNFCI*PSDNSIMSVLLATLCTRAIVPH 380
+++SSPL+ + F I PS S L TLCT+ I+P+
Sbjct: 32 SLSSSPLSKTQRF-ITPSQGS----RLRTLCTKVIIPN 64
>At5g49970.1 68418.m06188 pyridoxamine 5'-phosphate oxidase-related
contains weak similarity to Pyridoxamine 5'-phosphate
oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx).
(Swiss-Prot:P28225) [Shigella flexneri]
Length = 530
Score = 26.6 bits (56), Expect = 9.4
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -1
Query: 493 AVASSPLASFKNFCI*PSDNSIMSVLLATLCTRAIVPH 380
+++SSPL+ + F I PS S L TLCT+ I+P+
Sbjct: 32 SLSSSPLSKTQRF-ITPSQGS----RLRTLCTKVIIPN 64
>At5g01400.1 68418.m00053 expressed protein contains low similarity
to symplekin SP:Q92797 from [Homo sapiens]
Length = 1467
Score = 26.6 bits (56), Expect = 9.4
Identities = 16/66 (24%), Positives = 32/66 (48%)
Frame = +3
Query: 231 VPRPVLSVMLLFPISDAYENHKKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSV 410
+P+ VL ++ F + E K + + +++G ++ M+ I N C IAL +V
Sbjct: 785 LPKSVLMLLESFCCPGSGEVEKDLQHGDRVTQGLSAVWSLILMRPGIRNDCLNIALQSAV 844
Query: 411 ANNTDI 428
+ +I
Sbjct: 845 HHLEEI 850
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 26.6 bits (56), Expect = 9.4
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = -3
Query: 428 YVRIVGHTMYKSYSPTCIADILFHIE 351
++ ++ TM K + C DI+FH+E
Sbjct: 84 FIHLLVETMDKHFGNVCELDIMFHLE 109
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,513,172
Number of Sequences: 28952
Number of extensions: 210001
Number of successful extensions: 502
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 499
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -