Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS305F12f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g23800.1 68414.m03002 aldehyde dehydrogenase, mitochondrial (... 140 5e-34
At3g48000.1 68416.m05233 aldehyde dehydrogenase (ALDH2) identica... 139 1e-33
At3g24503.1 68416.m03074 aldehyde dehydrogenase (ALDH1a) identic... 121 3e-28
At3g48170.1 68416.m05254 betaine-aldehyde dehydrogenase, putativ... 89 2e-18
At1g79440.1 68414.m09258 succinate-semialdehyde dehydrogenase (S... 83 1e-16
At1g74920.1 68414.m08691 betaine-aldehyde dehydrogenase, putativ... 80 1e-15
At2g14170.1 68415.m01578 methylmalonate-semialdehyde dehydrogena... 58 3e-09
At2g24270.2 68415.m02900 NADP-dependent glyceraldehyde-3-phospha... 37 0.007
At2g24270.1 68415.m02899 NADP-dependent glyceraldehyde-3-phospha... 37 0.007
At2g15420.1 68415.m01764 myosin heavy chain-related 30 1.1
At1g53420.1 68414.m06054 serine/threonine protein kinase-related... 29 1.4
At2g23380.1 68415.m02792 curly leaf protein (CURLY LEAF) / polyc... 29 2.5
At3g01513.1 68416.m00078 expressed protein ; expression supporte... 27 5.8
At5g11510.1 68418.m01343 myb family transcription factor (MYB3R4... 27 7.7
At3g51550.1 68416.m05645 protein kinase family protein contains ... 27 7.7
>At1g23800.1 68414.m03002 aldehyde dehydrogenase, mitochondrial
(ALDH3) nearly identical to mitochondrial aldehyde
dehydrogenase ALDH3 [Arabidopsis thaliana]
gi|19850249|gb|AAL99612; contains Pfam profile PF00171:
aldehyde dehydrogenase (NAD) family protein
Length = 534
Score = 140 bits (339), Expect = 5e-34
Identities = 76/154 (49%), Positives = 96/154 (62%), Gaps = 1/154 (0%)
Frame = +3
Query: 54 VDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYS 233
V V++T+L I +VDAVS KTFPT++P++ VI QV+EGD D++ AVAAA+KAF
Sbjct: 49 VKVEHTQLLIGGRFVDAVSGKTFPTLDPRNGEVIAQVSEGDAEDVNRAVAAARKAFDE-G 107
Query: 234 PWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEE-EVYFSASVLRYYAG 410
PW M A +R + +A LET D GKP +QS + EV A V RYYAG
Sbjct: 108 PWPKMTAYERSKILFRFADLIEKHNDEIAALETWDNGKPYEQSAQIEVPMLARVFRYYAG 167
Query: 411 KADKILGNTIPSDGEVLTFTMKEPVGVCGQIIPW 512
ADKI G T+P DG T+ EP+GV GQIIPW
Sbjct: 168 WADKIHGMTMPGDGPHHVQTLHEPIGVAGQIIPW 201
>At3g48000.1 68416.m05233 aldehyde dehydrogenase (ALDH2) identical
to aldehyde dehydrogenase [Arabidopsis thaliana]
GI:8574427; similar to mitochondrial aldehyde
dehydrogenase [Arabidopsis thaliana]
gi|19850249|gb|AAL99612; identical to cDNA aldehyde
dehydrogenase AtALDH2a GI:20530140
Length = 538
Score = 139 bits (336), Expect = 1e-33
Identities = 78/160 (48%), Positives = 94/160 (58%), Gaps = 1/160 (0%)
Frame = +3
Query: 36 IINMVKVDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKK 215
IIN V V +T+L IN +VD+ S KTFPT++P+ VI VAEGD DI+ AV AA+
Sbjct: 48 IINP-SVQVSHTQLLINGNFVDSASGKTFPTLDPRTGEVIAHVAEGDAEDINRAVKAART 106
Query: 216 AFHRYSPWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQS-EEEVYFSASV 392
AF PW M A +R S LA LET D GKP +QS E+ A +
Sbjct: 107 AFDE-GPWPKMSAYERSRVLLRFADLVEKHSEELASLETWDNGKPYQQSLTAEIPMFARL 165
Query: 393 LRYYAGKADKILGNTIPSDGEVLTFTMKEPVGVCGQIIPW 512
RYYAG ADKI G TIP+DG T+ EP+GV GQIIPW
Sbjct: 166 FRYYAGWADKIHGLTIPADGNYQVHTLHEPIGVAGQIIPW 205
>At3g24503.1 68416.m03074 aldehyde dehydrogenase (ALDH1a) identical
to aldehyde dehydrogenase ALDH1a [Arabidopsis thaliana]
gi|20530143|gb|AAM27004
Length = 501
Score = 121 bits (291), Expect = 3e-28
Identities = 69/165 (41%), Positives = 97/165 (58%), Gaps = 3/165 (1%)
Frame = +3
Query: 27 KRNIINMVKV-DVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVA 203
K N VK+ ++K+TKLFIN +++DA S KTF TI+P++ VI +AEGDK D+DLAV
Sbjct: 5 KCNGATTVKLPEIKFTKLFINGQFIDAASGKTFETIDPRNGEVIATIAEGDKEDVDLAVN 64
Query: 204 AAKKAFHRYSPWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSE-EEVYF 380
AA+ AF + PW M +R LA+L+ +D GK + + ++
Sbjct: 65 AARYAFD-HGPWPRMTGFERAKLINKFADLIEENIEELAKLDAVDGGKLFQLGKYADIPA 123
Query: 381 SASVLRYYAGKADKILGNTIPSDGEVL-TFTMKEPVGVCGQIIPW 512
+A RY AG ADKI G T+ + L +T+KEP+GV G IIPW
Sbjct: 124 TAGHFRYNAGAADKIHGETLKMTRQSLFGYTLKEPIGVVGNIIPW 168
>At3g48170.1 68416.m05254 betaine-aldehyde dehydrogenase, putative
similar to betaine-aldehyde dehydrogenase, chloroplast
precursor (BADH) [Arabidopsis thaliana]
SWISS-PROT:Q9S795
Length = 503
Score = 89.0 bits (211), Expect = 2e-18
Identities = 50/160 (31%), Positives = 77/160 (48%), Gaps = 7/160 (4%)
Frame = +3
Query: 54 VDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYS 233
+ V +LFI +W + V +KT P +NP E +I + D++LAV AA+KAF R +
Sbjct: 3 ITVPRRQLFIGGQWTEPVLRKTLPVVNPATEDIIGYIPAATSEDVELAVEAARKAFTRNN 62
Query: 234 --PWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYA 407
W + R + LA LE +DCGKP+ ++ ++ A YYA
Sbjct: 63 GKDWARATGAVRAKYLRAIAAKVIERKSELANLEAIDCGKPLDEAAWDMDDVAGCFEYYA 122
Query: 408 GKADKILGN-----TIPSDGEVLTFTMKEPVGVCGQIIPW 512
A+ + ++P D + +KEP+GV G I PW
Sbjct: 123 DLAEGLDAKQKTPLSLPMD-TFKGYILKEPIGVVGMITPW 161
>At1g79440.1 68414.m09258 succinate-semialdehyde dehydrogenase
(SSADH1) similar to succinate-semialdehyde dehydrogenase
[NADP+] (SSDH) [Escherichia coli] SWISS-PROT:P25526;
identical to succinic semialdehyde dehydrogenase mRNA,
nuclear gene encoding mitochondrial protein GI:6684441;
contains TIGRfam profile TIGR01780:succinic semialdehyde
dehydrogenase; contains Pfam profile PF00171: aldehyde
dehydrogenase (NAD) family protein
Length = 528
Score = 82.6 bits (195), Expect = 1e-16
Identities = 46/149 (30%), Positives = 76/149 (51%), Gaps = 1/149 (0%)
Frame = +3
Query: 69 TKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYSPWRTM 248
T+ I +W+D+ KT NP +I VA + + A+A++ +AF + W +
Sbjct: 53 TQGLIGGKWLDSYDNKTIKVNNPATGEIIADVACMGTKETNDAIASSYEAF---TSWSRL 109
Query: 249 DASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADKIL 428
A +R + L +L TL+ GKP+K++ EV + AS + YYA +A ++
Sbjct: 110 TAGERSKVLRRWYDLLIAHKEELGQLITLEQGKPLKEAIGEVAYGASFIEYYAEEAKRVY 169
Query: 429 GNTIPSD-GEVLTFTMKEPVGVCGQIIPW 512
G+ IP + + +K+PVGV G I PW
Sbjct: 170 GDIIPPNLSDRRLLVLKQPVGVVGAITPW 198
>At1g74920.1 68414.m08691 betaine-aldehyde dehydrogenase, putative
identical to betaine-aldehyde dehydrogenase, chloroplast
precursor (BADH) [Arabidopsis thaliana]
SWISS-PROT:Q9S795; strong similarity to betaine aldehyde
dehydrogenase [Amaranthus hypochondriacus] GI:2388710
Length = 501
Score = 79.8 bits (188), Expect = 1e-15
Identities = 46/154 (29%), Positives = 75/154 (48%), Gaps = 7/154 (4%)
Frame = +3
Query: 72 KLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRY--SPWRT 245
+LFI+ EW + + KK P +NP E VI + D+D+AV AA++A R W
Sbjct: 9 QLFIDGEWREPILKKRIPIVNPATEEVIGDIPAATTEDVDVAVNAARRALSRNKGKDWAK 68
Query: 246 MDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADKI 425
+ R + LA+LE LDCGKP+ ++ ++ A +YA A+ +
Sbjct: 69 APGAVRAKYLRAIAAKVNERKTDLAKLEALDCGKPLDEAVWDMDDVAGCFEFYADLAEGL 128
Query: 426 LGN-----TIPSDGEVLTFTMKEPVGVCGQIIPW 512
++P + ++ +K+P+GV G I PW
Sbjct: 129 DAKQKAPVSLPME-SFKSYVLKQPLGVVGLITPW 161
>At2g14170.1 68415.m01578 methylmalonate-semialdehyde dehydrogenase,
putative similar to methylmalonate-semialdehyde
dehydrogenase [acylating], mitochondrial precursor
(MMSDH) [Rattus norvegicus] SWISS-PROT:Q02253
Length = 607
Score = 58.4 bits (135), Expect = 3e-09
Identities = 39/145 (26%), Positives = 66/145 (45%), Gaps = 1/145 (0%)
Frame = +3
Query: 81 INNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYSPWRTMDASQ 260
I +V++ S INP + V+++V + AV+AAK+AF WR +
Sbjct: 118 IGGSFVESQSSSFIDVINPATQEVVSKVPLTTNEEFKAAVSAAKQAF---PLWRNTPITT 174
Query: 261 RGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADKILGNTI 440
R LA T + GK +K S +++ V+ + G A +G +
Sbjct: 175 RQRVMLKFQELIRKNMDKLAMNITTEQGKTLKDSHGDIFRGLEVVEHACGMATLQMGEYL 234
Query: 441 PS-DGEVLTFTMKEPVGVCGQIIPW 512
P+ V T++++EP+GVC I P+
Sbjct: 235 PNVSNGVDTYSIREPLGVCAGICPF 259
>At2g24270.2 68415.m02900 NADP-dependent glyceraldehyde-3-phosphate
dehydrogenase, putative similar to NADP-dependent
glyceraldehyde-3-phosphate dehydrogenase
(NON-phosphorylating glyceraldehyde 3-phosphate;
glyceraldehyde-3-phosphate dehydrogenase [NADP+])
[Nicotiana plumbaginifolia] SWISS-PROT:P93338
Length = 496
Score = 37.1 bits (82), Expect = 0.007
Identities = 37/161 (22%), Positives = 62/161 (38%), Gaps = 8/161 (4%)
Frame = +3
Query: 54 VDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYS 233
+D + K + + EW + S K+ +NP +V + +++ + AK A
Sbjct: 11 LDGEVYKYYADGEWKTSSSGKSVAIMNPATRKTQYKVQACTQEEVNAVMELAKSA---QK 67
Query: 234 PWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGK 413
W +R +AE + KP K S EV S ++ Y A +
Sbjct: 68 SWAKTPLWKRAELLHKAAAILKDNKAPMAESLVKEIAKPAKDSVTEVVRSGDLISYCAEE 127
Query: 414 ADKILG-------NTIP-SDGEVLTFTMKEPVGVCGQIIPW 512
+ILG ++ P +D T K P+GV I P+
Sbjct: 128 GVRILGEGKFLLSDSFPGNDRTKYCLTSKIPLGVVLAIPPF 168
>At2g24270.1 68415.m02899 NADP-dependent glyceraldehyde-3-phosphate
dehydrogenase, putative similar to NADP-dependent
glyceraldehyde-3-phosphate dehydrogenase
(NON-phosphorylating glyceraldehyde 3-phosphate;
glyceraldehyde-3-phosphate dehydrogenase [NADP+])
[Nicotiana plumbaginifolia] SWISS-PROT:P93338
Length = 496
Score = 37.1 bits (82), Expect = 0.007
Identities = 37/161 (22%), Positives = 62/161 (38%), Gaps = 8/161 (4%)
Frame = +3
Query: 54 VDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRYS 233
+D + K + + EW + S K+ +NP +V + +++ + AK A
Sbjct: 11 LDGEVYKYYADGEWKTSSSGKSVAIMNPATRKTQYKVQACTQEEVNAVMELAKSA---QK 67
Query: 234 PWRTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGK 413
W +R +AE + KP K S EV S ++ Y A +
Sbjct: 68 SWAKTPLWKRAELLHKAAAILKDNKAPMAESLVKEIAKPAKDSVTEVVRSGDLISYCAEE 127
Query: 414 ADKILG-------NTIP-SDGEVLTFTMKEPVGVCGQIIPW 512
+ILG ++ P +D T K P+GV I P+
Sbjct: 128 GVRILGEGKFLLSDSFPGNDRTKYCLTSKIPLGVVLAIPPF 168
>At2g15420.1 68415.m01764 myosin heavy chain-related
Length = 957
Score = 29.9 bits (64), Expect = 1.1
Identities = 22/72 (30%), Positives = 31/72 (43%)
Frame = -2
Query: 493 HTPTGSFMVNVRTSPSEGMVFPNILSAFPA*YLSTLAEKYTSSSDCFTGFPQSKVSSSAR 314
H PTGSF +T P G + P L +++ + D G S V + AR
Sbjct: 584 HWPTGSFPETRQTRPRMGKIIPQ---------LPRFVDEFGENPDLMNGVEPSPVVTEAR 634
Query: 313 YLDWDSRSSASL 278
LD ++R SL
Sbjct: 635 -LDVENRGDESL 645
>At1g53420.1 68414.m06054 serine/threonine protein kinase-related
contains 1 predicted transmembrane domain; low
similarity to receptor-like serine/threonine kinase
[Arabidopsis thaliana] GI:2465923
Length = 953
Score = 29.5 bits (63), Expect = 1.4
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = -1
Query: 206 CGDGQINIRLISFSDLCYDCFILWVDGREGFFADSIYPFIVDK 78
CG +++I + YD W + R G+F++++ F+ DK
Sbjct: 370 CGGDEMSINGTIYESDKYDRLESWYESRNGWFSNNVGVFVDDK 412
>At2g23380.1 68415.m02792 curly leaf protein (CURLY LEAF) /
polycomb-group protein identical to polycomb group
[Arabidopsis thaliana] GI:1903019 (curly leaf); contains
Pfam profile PF00856: SET domain
Length = 902
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 359 IRRRSILFRKRAQILRWKSGQDIRKYHSFRRR 454
+RRRS R+R ++ R K YHS R+R
Sbjct: 609 VRRRSRFLRRRGKVRRLKYTWKSAAYHSIRKR 640
>At3g01513.1 68416.m00078 expressed protein ; expression supported
by MPSS
Length = 216
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/24 (45%), Positives = 18/24 (75%)
Frame = +3
Query: 108 SKKTFPTINPQDETVITQVAEGDK 179
SK++ PT+ +++TV +V EGDK
Sbjct: 169 SKRSSPTVKVKNKTVSYKVGEGDK 192
>At5g11510.1 68418.m01343 myb family transcription factor (MYB3R4)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 961
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 358 NPKKKYTFP-QACSDITLEKRTRY*EIPFLQTEKSLRS 468
+P + Y F Q SDI+LE R EIP T++S S
Sbjct: 312 SPSQDYQFDFQELSDISLEMRHNMSEIPMPYTKESKES 349
>At3g51550.1 68416.m05645 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 895
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +3
Query: 57 DVKYTKLFINNEWVDAVS----KKTFPTINPQDETVITQVAEGDKADID 191
DVK T + ++ +WV VS KT PT+ D T ++ V +G +D
Sbjct: 661 DVKTTNILLDEKWVAKVSDFGLSKTGPTL---DHTHVSTVVKGSFGYLD 706
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,632,764
Number of Sequences: 28952
Number of extensions: 239625
Number of successful extensions: 686
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 658
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 671
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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