Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS305F01f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g66070.1 68414.m07499 translation initiation factor-related s... 54 6e-08
At5g37475.1 68418.m04510 translation initiation factor-related s... 42 3e-04
At4g26120.1 68417.m03760 ankyrin repeat family protein / BTB/POZ... 29 1.9
At4g27850.1 68417.m03999 proline-rich family protein contains pr... 28 3.3
At3g47440.1 68416.m05158 major intrinsic family protein / MIP fa... 28 3.3
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 28 3.3
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 28 4.4
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 27 5.8
At3g25440.1 68416.m03163 group II intron splicing factor CRS1-re... 27 5.8
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 27 5.8
At5g19630.1 68418.m02336 expressed protein 27 7.7
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 27 7.7
>At1g66070.1 68414.m07499 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 226
Score = 54.0 bits (124), Expect = 6e-08
Identities = 27/70 (38%), Positives = 44/70 (62%)
Frame = +3
Query: 303 KTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTKEEYTEFAD 482
K A + P +AEKLRQQ+L EE+D R E FGV + + LD F P ++ ++ E+A+
Sbjct: 76 KEAPKEKPLDPIAEKLRQQRLVEEADYRATAELFGVKDDD-KNLDMFIPKSESDFLEYAE 134
Query: 483 LLTKKITFYK 512
+++ +I Y+
Sbjct: 135 MISHRIKPYE 144
>At5g37475.1 68418.m04510 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 225
Score = 41.9 bits (94), Expect = 3e-04
Identities = 34/149 (22%), Positives = 65/149 (43%), Gaps = 2/149 (1%)
Frame = +3
Query: 60 WDADNFEPKLPTTLAASNKWEGEDEDD-NVXXXXXXXXXXXXXXXXXXAAXXXXXXXXXX 236
W+A++F+P LP+ + + W+ ED D+ ++ A+
Sbjct: 4 WEAEDFQP-LPSKVELKSNWDDEDVDENDIKDSWEEEDVSAPPPIVKPASEKAPKKPAVK 62
Query: 237 IHDKIXXXXXXXXXXXXXLVTEKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGV-T 413
+K + K P +AEKLR Q+L EE+D + E FGV T
Sbjct: 63 AVEK----------KVKTVEAPKGTSREEPLDPIAEKLRMQRLVEEADYQSTAELFGVKT 112
Query: 414 EGNIGKLDNFHPTTKEEYTEFADLLTKKI 500
E +D P ++ ++ ++A+L+++++
Sbjct: 113 EEK--SVDMLIPKSESDFLDYAELISQRL 139
>At4g26120.1 68417.m03760 ankyrin repeat family protein / BTB/POZ
domain-containing protein contains Pfam domain, PF00023:
Ankyrin repeat and Pfam domain, PF00651: BTB/POZ domain
Length = 600
Score = 29.1 bits (62), Expect = 1.9
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +3
Query: 306 TAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTT 455
+ EE TPE++L +K R +LQE M+TF + GK PT+
Sbjct: 527 SVEEDTPEKRLQKKQRYMELQE-----TLMKTFSEDKEECGKSSTPKPTS 571
>At4g27850.1 68417.m03999 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 577
Score = 28.3 bits (60), Expect = 3.3
Identities = 15/38 (39%), Positives = 21/38 (55%)
Frame = +2
Query: 296 DREDCRRNDSRAEVG*ETSPAEATGRIRFATSHGNLWC 409
+RE+ N+S+ E SP EA IR +H NL+C
Sbjct: 28 EREENNNNESQEETRTSRSPNEALDEIRRQQTH-NLYC 64
>At3g47440.1 68416.m05158 major intrinsic family protein / MIP
family protein contains Pfam profile: MIP PF00230
Length = 256
Score = 28.3 bits (60), Expect = 3.3
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = -2
Query: 481 SANSVYSSFVVGWKLSSLPMLPSVTPKVSMASRKSDSSCSFCWRSFSANFCSGVISSAVF 302
+A ++ SS + W +S + P+VT +++A R S + F W S + V++ V
Sbjct: 67 NALALSSSVYISWNVSGGHVNPAVTFAMAVAGRISVPTAMFYWTS---QMIASVMACLVL 123
Query: 301 SVT 293
VT
Sbjct: 124 KVT 126
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -1
Query: 137 ILVFPFPFVGGCQRGREFGLEV 72
++VFP F+GG RG FGL V
Sbjct: 53 LVVFPEAFIGGYPRGFRFGLGV 74
>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
family protein contains 3 WD-40 repeats (PF00400); some
similarity to s-tomosyn isoform (GI:4689231)[Rattus
norvegicus]; contains non-consensus AT-AC splice sites at
intron 18
Length = 1606
Score = 27.9 bits (59), Expect = 4.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 18 NSAADSGVLKMDVSWDADNFEPKLPTTLAASNKWE 122
N AA+S V K W N+E K PT + + KWE
Sbjct: 991 NFAAESMVPK-GPEWGGGNWEIKTPTDMKSIPKWE 1024
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 137 ILVFPFPFVGGCQRGREFGLEV 72
+++FP F+GG RG FGL V
Sbjct: 60 LVLFPEAFIGGYPRGFRFGLAV 81
>At3g25440.1 68416.m03163 group II intron splicing factor
CRS1-related contains weak similarity to CRS1 [Zea mays]
gi|9837550|gb|AAG00595
Length = 380
Score = 27.5 bits (58), Expect = 5.8
Identities = 24/73 (32%), Positives = 35/73 (47%)
Frame = +3
Query: 300 EKTAEEMTPEQKLAEKLRQQKLQEESDLRLAMETFGVTEGNIGKLDNFHPTTKEEYTEFA 479
+K E MT E+K+ KLR+ + +EE RL MET + P+ E T
Sbjct: 65 KKEFERMTSEEKILNKLRKARKKEE---RL-MET----------MKKLEPSESAETTHDP 110
Query: 480 DLLTKKITFYKAK 518
++LT + FY K
Sbjct: 111 EILTPEEHFYYLK 123
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/30 (33%), Positives = 21/30 (70%)
Frame = +3
Query: 291 LVTEKTAEEMTPEQKLAEKLRQQKLQEESD 380
+V EKT E+ PE+++ E+ + ++ EE++
Sbjct: 150 IVEEKTEEKTEPEEEIKEETKPEEENEEAE 179
>At5g19630.1 68418.m02336 expressed protein
Length = 228
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -1
Query: 143 VIILVFPFPFVGGCQ 99
VI+LV PF +GGCQ
Sbjct: 41 VIVLVHPFSLLGGCQ 55
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 137 ILVFPFPFVGGCQRGREFGLEV 72
+++FP F+GG RG FGL V
Sbjct: 60 LVLFPEGFIGGYPRGFRFGLAV 81
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,771,474
Number of Sequences: 28952
Number of extensions: 137718
Number of successful extensions: 579
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 579
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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