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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS305B01f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putativ...    40   0.001
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    40   0.001
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-...    40   0.001
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl...    36   0.017
At1g24290.1 68414.m03065 AAA-type ATPase family protein similar ...    33   0.088
At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N domain-...    29   1.4  
At1g74250.1 68414.m08599 DNAJ heat shock N-terminal domain-conta...    28   3.3  

>At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putative
           (UBP14) similar to ubiquitin-specific protease 14
           GI:11993473 [Arabidopsis thaliana]
          Length = 797

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 17/39 (43%), Positives = 24/39 (61%)
 Frame = +3

Query: 81  IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAED 197
           +  L+ MGF +   +KA   T+  GVE AM WLL+H +D
Sbjct: 619 VAQLVSMGFSQLHCQKAAINTSNAGVEEAMNWLLSHMDD 657



 Score = 31.1 bits (67), Expect = 0.47
 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 4/63 (6%)
 Frame = +3

Query: 75  AEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLL----AHAEDLAVSSEPSNSQAGES 242
           + + TL+  GF ++ A KAL  +    +E A +W+     A   D+ VSS  S     +S
Sbjct: 674 SSVDTLLSFGFAEDVARKALKASG-GDIEKATDWVFNNPNASVSDMDVSSSNSAQTPAQS 732

Query: 243 SAP 251
             P
Sbjct: 733 GLP 735


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
            identical to ubiquitin-protein ligase 2 [Arabidopsis
            thaliana] GI:7108523; E3, HECT-domain protein family;
            similar to ubiquitin-protein ligase 2 GI:7108523 from
            [Arabidopsis thaliana]
          Length = 3658

 Score = 39.5 bits (88), Expect = 0.001
 Identities = 18/41 (43%), Positives = 24/41 (58%)
 Frame = +3

Query: 75   AEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAED 197
            A +  ++EMGF + RAE AL       VE AM+WL  + ED
Sbjct: 1275 AIVGMIVEMGFSRSRAEDALRRVGTNSVEMAMDWLFTNPED 1315


>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
           domain-containing protein weak similarity to SP|P45974
           Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
           {Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
           domain
          Length = 413

 Score = 39.5 bits (88), Expect = 0.001
 Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
 Frame = +3

Query: 81  IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAEDLAVS---SEPSNSQAG 236
           ++ L  MGFPK RA +AL  +    +E A+ W++ H  D  V      PSNS  G
Sbjct: 116 LEELEAMGFPKARATRALHYSGNASLEAAVNWVVEHENDPDVDEMPKVPSNSNVG 170



 Score = 38.3 bits (85), Expect = 0.003
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +3

Query: 315 SLKCDECGKLFKNQDEIEYHAAKTNHSSF 401
           SLKC +CG L K+ +E + HA  T+HS+F
Sbjct: 5   SLKCGDCGTLLKSVEEAQEHAELTSHSNF 33


>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
            identical to ubiquitin-protein ligase 1 [Arabidopsis
            thaliana] GI:7108521; E3, HECT-domain protein family;
            similar to GI:7108521, GB:AAF36454 from [Arabidopsis
            thaliana]
          Length = 3891

 Score = 35.9 bits (79), Expect = 0.017
 Identities = 16/35 (45%), Positives = 21/35 (60%)
 Frame = +3

Query: 90   LIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAE 194
            ++EMGF + RAE AL       VE AM+WL  + E
Sbjct: 1527 IVEMGFSRSRAEIALRRVGTNSVEMAMDWLFTNPE 1561


>At1g24290.1 68414.m03065 AAA-type ATPase family protein similar to
           Werner helicase interacting protein [Homo sapiens]
           GI:14349166; contains Pfam profiles PF00004: ATPase
           family associated with various cellular activities
           (AAA), PF00627: UBA/TS-N domain; contains
           ATP/GTP-binding site motif A (P-loop)
          Length = 525

 Score = 33.5 bits (73), Expect = 0.088
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = +3

Query: 81  IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAH 188
           ++ L+ MGF  + A +AL  T    ++ A +W+L+H
Sbjct: 1   MEQLVSMGFSSDLAAEALTATGGDSIQKATDWILSH 36


>At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N
           domain-containing protein contains Pfam profile PF00627:
           UBA/TS-N domain
          Length = 619

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 15/43 (34%), Positives = 22/43 (51%)
 Frame = +3

Query: 51  YYLQT*IMAEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWL 179
           Y +   +    Q +I MG P ERA  AL +   K +E ++ WL
Sbjct: 148 YLMSRKLEVHAQLIISMGIPHERATYALMLNEGK-IEESINWL 189


>At1g74250.1 68414.m08599 DNAJ heat shock N-terminal
           domain-containing protein contains Pfam domains PF00226:
           DnaJ domain and PF00096: Zinc finger, C2H2 type
          Length = 630

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 9/28 (32%), Positives = 19/28 (67%)
 Frame = +3

Query: 315 SLKCDECGKLFKNQDEIEYHAAKTNHSS 398
           S +CD CG+ F+++ ++  H A + H++
Sbjct: 599 SNECDRCGEEFESRTKLHKHLADSGHAT 626


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,515,499
Number of Sequences: 28952
Number of extensions: 145681
Number of successful extensions: 415
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 415
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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