BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS305B01f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putativ... 40 0.001 At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 40 0.001 At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 40 0.001 At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 36 0.017 At1g24290.1 68414.m03065 AAA-type ATPase family protein similar ... 33 0.088 At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N domain-... 29 1.4 At1g74250.1 68414.m08599 DNAJ heat shock N-terminal domain-conta... 28 3.3 >At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putative (UBP14) similar to ubiquitin-specific protease 14 GI:11993473 [Arabidopsis thaliana] Length = 797 Score = 39.9 bits (89), Expect = 0.001 Identities = 17/39 (43%), Positives = 24/39 (61%) Frame = +3 Query: 81 IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAED 197 + L+ MGF + +KA T+ GVE AM WLL+H +D Sbjct: 619 VAQLVSMGFSQLHCQKAAINTSNAGVEEAMNWLLSHMDD 657 Score = 31.1 bits (67), Expect = 0.47 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 4/63 (6%) Frame = +3 Query: 75 AEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLL----AHAEDLAVSSEPSNSQAGES 242 + + TL+ GF ++ A KAL + +E A +W+ A D+ VSS S +S Sbjct: 674 SSVDTLLSFGFAEDVARKALKASG-GDIEKATDWVFNNPNASVSDMDVSSSNSAQTPAQS 732 Query: 243 SAP 251 P Sbjct: 733 GLP 735 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 39.5 bits (88), Expect = 0.001 Identities = 18/41 (43%), Positives = 24/41 (58%) Frame = +3 Query: 75 AEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAED 197 A + ++EMGF + RAE AL VE AM+WL + ED Sbjct: 1275 AIVGMIVEMGFSRSRAEDALRRVGTNSVEMAMDWLFTNPED 1315 >At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-containing protein weak similarity to SP|P45974 Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15) {Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N domain Length = 413 Score = 39.5 bits (88), Expect = 0.001 Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 3/55 (5%) Frame = +3 Query: 81 IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAEDLAVS---SEPSNSQAG 236 ++ L MGFPK RA +AL + +E A+ W++ H D V PSNS G Sbjct: 116 LEELEAMGFPKARATRALHYSGNASLEAAVNWVVEHENDPDVDEMPKVPSNSNVG 170 Score = 38.3 bits (85), Expect = 0.003 Identities = 15/29 (51%), Positives = 21/29 (72%) Frame = +3 Query: 315 SLKCDECGKLFKNQDEIEYHAAKTNHSSF 401 SLKC +CG L K+ +E + HA T+HS+F Sbjct: 5 SLKCGDCGTLLKSVEEAQEHAELTSHSNF 33 >At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly identical to ubiquitin-protein ligase 1 [Arabidopsis thaliana] GI:7108521; E3, HECT-domain protein family; similar to GI:7108521, GB:AAF36454 from [Arabidopsis thaliana] Length = 3891 Score = 35.9 bits (79), Expect = 0.017 Identities = 16/35 (45%), Positives = 21/35 (60%) Frame = +3 Query: 90 LIEMGFPKERAEKALAVTNYKGVEPAMEWLLAHAE 194 ++EMGF + RAE AL VE AM+WL + E Sbjct: 1527 IVEMGFSRSRAEIALRRVGTNSVEMAMDWLFTNPE 1561 >At1g24290.1 68414.m03065 AAA-type ATPase family protein similar to Werner helicase interacting protein [Homo sapiens] GI:14349166; contains Pfam profiles PF00004: ATPase family associated with various cellular activities (AAA), PF00627: UBA/TS-N domain; contains ATP/GTP-binding site motif A (P-loop) Length = 525 Score = 33.5 bits (73), Expect = 0.088 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = +3 Query: 81 IQTLIEMGFPKERAEKALAVTNYKGVEPAMEWLLAH 188 ++ L+ MGF + A +AL T ++ A +W+L+H Sbjct: 1 MEQLVSMGFSSDLAAEALTATGGDSIQKATDWILSH 36 >At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627: UBA/TS-N domain Length = 619 Score = 29.5 bits (63), Expect = 1.4 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +3 Query: 51 YYLQT*IMAEIQTLIEMGFPKERAEKALAVTNYKGVEPAMEWL 179 Y + + Q +I MG P ERA AL + K +E ++ WL Sbjct: 148 YLMSRKLEVHAQLIISMGIPHERATYALMLNEGK-IEESINWL 189 >At1g74250.1 68414.m08599 DNAJ heat shock N-terminal domain-containing protein contains Pfam domains PF00226: DnaJ domain and PF00096: Zinc finger, C2H2 type Length = 630 Score = 28.3 bits (60), Expect = 3.3 Identities = 9/28 (32%), Positives = 19/28 (67%) Frame = +3 Query: 315 SLKCDECGKLFKNQDEIEYHAAKTNHSS 398 S +CD CG+ F+++ ++ H A + H++ Sbjct: 599 SNECDRCGEEFESRTKLHKHLADSGHAT 626 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,515,499 Number of Sequences: 28952 Number of extensions: 145681 Number of successful extensions: 415 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 403 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 415 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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