Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS304E04f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 157 4e-39
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 153 7e-38
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 99 2e-21
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 99 2e-21
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 77 5e-15
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 73 1e-13
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 71 4e-13
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 70 8e-13
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 67 6e-12
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 64 7e-11
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 62 3e-10
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 46 2e-05
At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1) (P... 34 0.050
At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2) (... 33 0.12
At3g26010.1 68416.m03240 F-box family protein contains Pfam:PF00... 29 1.4
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 27 5.8
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 157 bits (381), Expect = 4e-39
Identities = 74/145 (51%), Positives = 101/145 (69%)
Frame = -3
Query: 519 QYKYGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYC 340
++ YG E+PV IL + IAD SQVYTQ+A MRPLG M++ D+E GP YK DP+G++
Sbjct: 102 RFTYGYEMPVDILAKWIADKSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFY 161
Query: 339 SYKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVS 160
+KA +AG K + +LEKK+K + D+ +Q AIS L VL DFK++EIE+GVV
Sbjct: 162 GHKATSAGMKEQEAVNFLEKKMKENPSFTFDETVQTAISALQSVLQEDFKATEIEVGVVR 221
Query: 159 KDNPKFRVLTETEIDRHLTAIAEKD 85
+NP+FR LT EI+ HLTAI+E+D
Sbjct: 222 AENPEFRALTTEEIEEHLTAISERD 246
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 153 bits (371), Expect = 7e-38
Identities = 73/145 (50%), Positives = 99/145 (68%)
Frame = -3
Query: 519 QYKYGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYC 340
+++YG E+P IL + IAD SQVYTQ+A MRPLG M++ D+E GP YK DP+G++
Sbjct: 102 RFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGIDEERGPLLYKCDPAGHFY 161
Query: 339 SYKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVS 160
+KA +AG K + +LEKK+K + D+ +Q AIS L VL DFK++EIE+GVV
Sbjct: 162 GHKATSAGMKEQEAVNFLEKKMKENPAFTYDETVQTAISALQSVLQEDFKATEIEVGVVR 221
Query: 159 KDNPKFRVLTETEIDRHLTAIAEKD 85
D+P FR L EID HLTAI+E+D
Sbjct: 222 ADDPLFRSLRTEEIDEHLTAISERD 246
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 99.1 bits (236), Expect = 2e-21
Identities = 56/138 (40%), Positives = 75/138 (54%)
Frame = -3
Query: 510 YGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYK 331
Y IPV L R A + Q +TQ+ +RP G S+++ YDD+ GP Y+ DPSG Y S+K
Sbjct: 101 YKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYDDK-GPQLYQVDPSGSYFSWK 159
Query: 330 AVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVSKDN 151
A A G + +LEK+ D+ DDAI AI L + + S IEIG + D
Sbjct: 160 ASAMGKNVSNAKTFLEKRYTE--DMELDDAIHTAILTLKEGFEGEISSKNIEIGKIGTDK 217
Query: 150 PKFRVLTETEIDRHLTAI 97
FRVLT EID +L +
Sbjct: 218 -VFRVLTPAEIDDYLAEV 234
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 99.1 bits (236), Expect = 2e-21
Identities = 56/138 (40%), Positives = 75/138 (54%)
Frame = -3
Query: 510 YGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYK 331
Y IPV L R A + Q +TQ+ +RP G S+++ YDD+ GP Y+ DPSG Y S+K
Sbjct: 101 YKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYDDK-GPQLYQVDPSGSYFSWK 159
Query: 330 AVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVSKDN 151
A A G + +LEK+ D+ DDAI AI L + + S IEIG + D
Sbjct: 160 ASAMGKNVSNAKTFLEKRYTE--DMELDDAIHTAILTLKEGFEGEISSKNIEIGKIGADK 217
Query: 150 PKFRVLTETEIDRHLTAI 97
FRVLT EID +L +
Sbjct: 218 -VFRVLTPAEIDDYLAEV 234
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 77.4 bits (182), Expect = 5e-15
Identities = 49/139 (35%), Positives = 76/139 (54%), Gaps = 2/139 (1%)
Frame = -3
Query: 498 IPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAG-PCXYKTDPSGYYCSYKAVA 322
+ V + R IA + Q YTQ+ +RP G S +++ +D + P Y+TDPSG + ++KA A
Sbjct: 103 VTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYSRLPSLYQTDPSGTFSAWKANA 162
Query: 321 AGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVSKDNPKF 142
G + +LEK K + S + I+LAI L +V V+ IE+ V++++
Sbjct: 163 TGRNSNSIREFLEKNYK---ESSGQETIKLAIRALLEV--VESGGKNIEVAVMTREETGL 217
Query: 141 RVLTETEIDRHLTAI-AEK 88
R L E EID + I AEK
Sbjct: 218 RQLEEAEIDAIVAKIEAEK 236
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 72.9 bits (171), Expect = 1e-13
Identities = 46/139 (33%), Positives = 75/139 (53%), Gaps = 2/139 (1%)
Frame = -3
Query: 498 IPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAG-PCXYKTDPSGYYCSYKAVA 322
+ V + R IA + Q YTQ+ +RP G S +++ +D P Y+TDPSG + ++KA A
Sbjct: 103 VTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYTRIPALYQTDPSGTFSAWKANA 162
Query: 321 AGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIEIGVVSKDNPKF 142
G + +LEK K + + + ++LAI L +V V+ IE+ V++++
Sbjct: 163 TGRNSNSIREFLEKNYK---ESAGQETVKLAIRALLEV--VESGGKNIEVAVMTREEGVL 217
Query: 141 RVLTETEIDRHLTAI-AEK 88
+ L E EID + I AEK
Sbjct: 218 KQLEEEEIDIIVAEIEAEK 236
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 71.3 bits (167), Expect = 4e-13
Identities = 46/136 (33%), Positives = 70/136 (51%), Gaps = 1/136 (0%)
Frame = -3
Query: 516 YKYGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCS 337
+ Y S +PV L +AD +QV TQ + RP G + L+ DE+G Y PSG Y
Sbjct: 97 FTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGL-LVGGLDESGAHLYYNCPSGNYFE 155
Query: 336 YKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVD-FKSSEIEIGVVS 160
Y+A A G+++ YLE+K + + S +D I+ AI + + L + KSS + V+
Sbjct: 156 YQAFAIGSRSQAAKTYLERKFESFQESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLG 215
Query: 159 KDNPKFRVLTETEIDR 112
D P F L + I +
Sbjct: 216 VDEP-FHFLDQESIQK 230
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 70.1 bits (164), Expect = 8e-13
Identities = 45/136 (33%), Positives = 70/136 (51%), Gaps = 1/136 (0%)
Frame = -3
Query: 516 YKYGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCS 337
+ Y S +PV L +AD +QV TQ + RP G + L+ DE+G Y PSG Y
Sbjct: 97 FTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGL-LVGGLDESGAHLYYNCPSGNYFE 155
Query: 336 YKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVD-FKSSEIEIGVVS 160
Y+A A G+++ YLE++ + D S +D I+ AI + + L + KSS + ++
Sbjct: 156 YQAFAIGSRSQAAKTYLERRFESFGDSSREDLIKDAILAVRETLQGETLKSSLCTVAILG 215
Query: 159 KDNPKFRVLTETEIDR 112
D P F L + I +
Sbjct: 216 VDEP-FHFLDQEAIQK 230
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 67.3 bits (157), Expect = 6e-12
Identities = 37/119 (31%), Positives = 61/119 (51%), Gaps = 1/119 (0%)
Frame = -3
Query: 516 YKYGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCS 337
+ Y +PV L + + D Q YTQ +RP G S + +D G Y +DPSG Y
Sbjct: 99 FMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYGG 158
Query: 336 YKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVL-SVDFKSSEIEIGVV 163
+KA A GA + L++ K D + ++A++LA+ L++ + S S ++E+ V
Sbjct: 159 WKAAAVGANNQAAQSILKQDYK--DDATREEAVELALKVLTKTMDSTSLTSEKLELAEV 215
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 63.7 bits (148), Expect = 7e-11
Identities = 42/140 (30%), Positives = 75/140 (53%), Gaps = 5/140 (3%)
Frame = -3
Query: 519 QYKYGSEIPVHILXRRIADISQVYTQNAE---MRPLGCSMMLIAYDDEAGPCXYKTDPSG 349
++ YG + V + + D++ + + E RP G S+ LIA DE GP Y TDPSG
Sbjct: 100 RFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSL-LIAGHDENGPSLYYTDPSG 158
Query: 348 --YYCSYKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIE 175
+ C+ KA+ +G++ D + L+++ + DLS +A +A+S L QV+ + ++
Sbjct: 159 TFWQCNAKAIGSGSEGADSS--LQEQFNK--DLSLQEAETIAVSILKQVMEEKVTPNNVD 214
Query: 174 IGVVSKDNPKFRVLTETEID 115
I V+ P + + T E++
Sbjct: 215 IAKVA---PAYHLYTPQEVE 231
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 61.7 bits (143), Expect = 3e-10
Identities = 40/140 (28%), Positives = 75/140 (53%), Gaps = 5/140 (3%)
Frame = -3
Query: 519 QYKYGSEIPVHILXRRIADISQVYTQNAE---MRPLGCSMMLIAYDDEAGPCXYKTDPSG 349
++ YG + V + + D++ + + E RP G S+ LIA DE GP Y TDPSG
Sbjct: 100 RFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSL-LIAGHDENGPSLYYTDPSG 158
Query: 348 --YYCSYKAVAAGAKTVDGNAYLEKKLKRRXDLSTDDAIQLAISCLSQVLSVDFKSSEIE 175
+ C+ KA+ +G++ D + L+++ + D++ +A +A+S L QV+ + ++
Sbjct: 159 TFWQCNAKAIGSGSEGADSS--LQEQFNK--DITLQEAETIAVSILKQVMEEKVTPNNVD 214
Query: 174 IGVVSKDNPKFRVLTETEID 115
I V+ P + + T E++
Sbjct: 215 IAKVA---PAYHLYTPQEVE 231
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 45.6 bits (103), Expect = 2e-05
Identities = 26/77 (33%), Positives = 35/77 (45%)
Frame = -3
Query: 510 YGSEIPVHILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYK 331
YG +PV L R+A + T +RP GC ++L YD + GP Y +PSG Y
Sbjct: 103 YGDAVPVKELSERVASYVHLCTLYWWLRPFGCGVILGGYDRD-GPQLYMIEPSGISYRYF 161
Query: 330 AVAAGAKTVDGNAYLEK 280
A G +EK
Sbjct: 162 GAAIGKGKQAAKTEIEK 178
>At3g22630.1 68416.m02857 20S proteasome beta subunit D (PBD1)
(PRGB) identical to GB:CAA74026 from [Arabidopsis
thaliana] ( FEBS Lett. (1997) 416 (3), 281-285);
identical to cDNA proteasome subunit prgb GI:2511589
Length = 204
Score = 34.3 bits (75), Expect = 0.050
Identities = 26/97 (26%), Positives = 45/97 (46%), Gaps = 3/97 (3%)
Frame = -3
Query: 426 PLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKR--RXDLS 253
P ++++ YDDE+G Y D Y + V GA G+ + + R R D+S
Sbjct: 96 PYSVNILMAGYDDESGASLYYID---YIATLHKVDKGAFGY-GSYFSLSTMDRHYRSDMS 151
Query: 252 TDDAIQLAISCLSQVLS-VDFKSSEIEIGVVSKDNPK 145
++AI+L C+ ++ S + I +V KD +
Sbjct: 152 VEEAIELVDKCILEIRSRLVVAPPNFVIKIVDKDGAR 188
>At4g14800.1 68417.m02275 20S proteasome beta subunit D2 (PBD2)
(PRCGA) identical to SP|O24633 Proteasome subunit beta
type 2-2 (EC 3.4.25.1) (20S proteasome alpha subunit D2)
{Arabidopsis thaliana}, cDNA proteasome subunit prcga
GI:2511571
Length = 199
Score = 33.1 bits (72), Expect = 0.12
Identities = 26/97 (26%), Positives = 44/97 (45%), Gaps = 3/97 (3%)
Frame = -3
Query: 426 PLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYKAVAAGAKTVDGNAYLEKKLKR--RXDLS 253
P ++++ YD EAG Y D Y + V GA G+ + + R R D+S
Sbjct: 96 PYSVNILMAGYDKEAGASLYYID---YIATLHKVDKGAFGY-GSYFSLSTMDRHYRSDMS 151
Query: 252 TDDAIQLAISCLSQVLS-VDFKSSEIEIGVVSKDNPK 145
++AI+L C+ ++ S + I +V KD +
Sbjct: 152 VEEAIELVDKCILEIRSRLVIAPPNFVIKIVDKDGAR 188
>At3g26010.1 68416.m03240 F-box family protein contains Pfam:PF00646
F-box domain
Length = 414
Score = 29.5 bits (63), Expect = 1.4
Identities = 17/68 (25%), Positives = 31/68 (45%)
Frame = -3
Query: 486 ILXRRIADISQVYTQNAEMRPLGCSMMLIAYDDEAGPCXYKTDPSGYYCSYKAVAAGAKT 307
+L ++ +++V N + CS+ + Y E G KT +C Y+ G+ T
Sbjct: 150 VLGFKVVKLAEVIVTN---NYVSCSLSVFVYSSETGIWTCKT----IHCPYQITNFGSFT 202
Query: 306 VDGNAYLE 283
+DG Y +
Sbjct: 203 LDGTIYFD 210
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/52 (28%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = -1
Query: 236 SLPSLVCLKCCLSTSSPLRLRLVLSAKIILSFGC-*LKLKSTDISRQSLKRI 84
+L +L +KC P + + K ++ FGC LK+ T+I+ SL+++
Sbjct: 650 NLETLTLIKCSSLVELPSSISNLQKLKALMMFGCKMLKVVPTNINLVSLEKV 701
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,150,741
Number of Sequences: 28952
Number of extensions: 153169
Number of successful extensions: 393
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 380
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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