Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS304D11f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 235 2e-62
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 233 4e-62
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 215 2e-56
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 214 3e-56
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 149 1e-36
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 143 6e-35
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 139 9e-34
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 1.9
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 1.9
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 29 1.9
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 2.5
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 2.5
At1g21440.1 68414.m02681 mutase family protein similar to carbox... 28 3.3
At3g63330.1 68416.m07125 protein kinase family protein contains ... 27 5.8
At4g13660.1 68417.m02124 pinoresinol-lariciresinol reductase, pu... 27 7.7
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 235 bits (574), Expect = 2e-62
Identities = 110/163 (67%), Positives = 129/163 (79%), Gaps = 2/163 (1%)
Frame = +3
Query: 39 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 212
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKGS+ V
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 213 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 392
D L +NG K+ V S+RDP IPW GA+YVVES+GVFTT KA++HL+GGAKKVIIS
Sbjct: 147 DDSTLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIIS 206
Query: 393 APSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 521
APSADAPMFVVGVN Y P+ ++SNASCTTNCLAPLAKV+H
Sbjct: 207 APSADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKVVH 249
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 233 bits (571), Expect = 4e-62
Identities = 109/163 (66%), Positives = 131/163 (80%), Gaps = 2/163 (1%)
Frame = +3
Query: 39 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV- 212
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 213 QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIIS 392
D L +NG ++ V S+RDP IPW GAEYVVES+GVFTT +AS+HL+GGAKKVIIS
Sbjct: 145 DDSTLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIIS 204
Query: 393 APSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 521
APSADAPMFVVGVN + Y P+ ++SNASCTTNCLAPLAKV+H
Sbjct: 205 APSADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKVVH 247
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 215 bits (525), Expect = 2e-56
Identities = 103/169 (60%), Positives = 132/169 (78%), Gaps = 3/169 (1%)
Frame = +3
Query: 24 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFKG 200
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 201 -SVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 374
++V+D ++ G K + VF R+P+ IPWG+AGA++VVESTGVFT DKA+AHL+GGA
Sbjct: 61 HELKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGA 120
Query: 375 KKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 521
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVIN 169
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 214 bits (523), Expect = 3e-56
Identities = 102/169 (60%), Positives = 131/169 (77%), Gaps = 3/169 (1%)
Frame = +3
Query: 24 LADNMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 197
+AD +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 198 GSVEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGA 374
++++D ++ G K + VF R+P+ IPW +AGA+YVVESTGVFT DKA+AHL+GGA
Sbjct: 61 NELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGA 120
Query: 375 KKVIISAPSADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIH 521
KKV+ISAPS DAPMFVVGVN Y ++SNASCTTNCLAPLAKVI+
Sbjct: 121 KKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVIN 169
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 149 bits (360), Expect = 1e-36
Identities = 81/164 (49%), Positives = 100/164 (60%), Gaps = 5/164 (3%)
Frame = +3
Query: 42 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 212
K+ INGFGRIGR LR + VV IND G+ +L KYDST G F V+
Sbjct: 66 KVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIFDADVKP 124
Query: 213 Q-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 389
D L V+G I + S+R+P +PWG+ G + V+E TGVF D A HL+ GAKKV+I
Sbjct: 125 SGDSALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLI 184
Query: 390 SAP-SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVI 518
+AP D P +VVGVN E Y +ISNASCTTNCLAP KV+
Sbjct: 185 TAPGKGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVL 228
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 143 bits (347), Expect = 6e-35
Identities = 78/164 (47%), Positives = 100/164 (60%), Gaps = 5/164 (3%)
Frame = +3
Query: 42 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 212
K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F V+
Sbjct: 63 KVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIFDADVKP 121
Query: 213 Q-DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 389
+ + V+G I V S R+P +PW + G + V+E TGVF + A H+E GAKKVII
Sbjct: 122 SGETAISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVII 181
Query: 390 SAP-SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVI 518
+AP D P +VVGVN +AY +ISNASCTTNCLAP KV+
Sbjct: 182 TAPGKGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVL 225
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 139 bits (337), Expect = 9e-34
Identities = 78/166 (46%), Positives = 101/166 (60%), Gaps = 7/166 (4%)
Frame = +3
Query: 42 KIGINGFGRIGRLVLRA---SIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEV 212
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK V++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 213 QDGFLV-VNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 389
D + V+G I V S RDP +PW + G + V+E TGVF A H++ GA KVII
Sbjct: 142 VDNETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVII 201
Query: 390 SAPS--ADAPMFVVGVNLEAYDPSF-KVISNASCTTNCLAPLAKVI 518
+AP+ AD P +V+GVN + Y +ISNASCTTNCLAP AKV+
Sbjct: 202 TAPAKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKVL 247
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 51 INGFGRIGRLVLRASIEKGAQVVAIND 131
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 51 INGFGRIGRLVLRASIEKGAQVVAIND 131
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 29.1 bits (62), Expect = 1.9
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 76 VWCSVLLLKRELKWSL*MTLSSVLTI 153
+WC+++LL+REL W + +V T+
Sbjct: 331 IWCALILLERELVWGVIEWSENVFTL 356
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -1
Query: 152 IVKTDERVIYSDHLSSLFNRSTEHQTANAAKS 57
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 2.5
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +3
Query: 42 KIGINGFGRIGRLVLRASIEKGAQVVAIND 131
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
>At1g21440.1 68414.m02681 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 336
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = +3
Query: 291 KAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLEAY 446
+ A + TG+ D+A+ ++E GA + AP D + +G + Y
Sbjct: 191 RTDARALSAKTGLSDAIDRANLYMEAGADASFVEAPRDDDELKEIGRRTKGY 242
>At3g63330.1 68416.m07125 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.5 bits (58), Expect = 5.8
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 325 VSLPLQIKHLLTWREVLKKLLYQLPVLMPPCLL 423
V+ L +H+ W E K+L Y+L LM C+L
Sbjct: 262 VTRALLDQHIRGWSENFKELAYKLRSLMEMCIL 294
>At4g13660.1 68417.m02124 pinoresinol-lariciresinol reductase,
putative similar to pinoresinol-lariciresinol reductase
TH1 [Tsuga heterophylla][GI:7578915]; contains
isoflavone reductase domain PF02716
Length = 317
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +3
Query: 51 INGFGRIGRLVLRASIEKGAQVVAINDPFIGLD 149
+ G G +GR ++ A + +G + + P IG+D
Sbjct: 15 VGGTGSLGRRIVSACLAEGHETYVLQRPEIGVD 47
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,346,130
Number of Sequences: 28952
Number of extensions: 263227
Number of successful extensions: 710
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 693
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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