BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS304D02f (521 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 355 e-100 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 354 e-100 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 340 5e-96 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 321 3e-90 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 8e-05 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 1.9 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 2.5 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 5.8 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 5.8 AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 21 7.7 AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase pro... 21 7.7 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 355 bits (874), Expect = e-100 Identities = 166/172 (96%), Positives = 168/172 (97%) Frame = +2 Query: 5 AWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 184 AWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 116 Query: 185 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 364 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSS Sbjct: 117 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSS 176 Query: 365 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 520 YIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 177 YIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 228 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 354 bits (870), Expect = e-100 Identities = 165/171 (96%), Positives = 167/171 (97%) Frame = +2 Query: 8 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 187 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60 Query: 188 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 367 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120 Query: 368 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 520 IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 171 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 340 bits (836), Expect = 5e-96 Identities = 158/172 (91%), Positives = 164/172 (95%) Frame = +2 Query: 5 AWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 184 AWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV Sbjct: 57 AWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 116 Query: 185 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 364 AAG GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSS Sbjct: 117 AAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSS 176 Query: 365 YIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 520 YIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VERK+G ADGK L Sbjct: 177 YIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTL 228 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 321 bits (788), Expect = 3e-90 Identities = 149/155 (96%), Positives = 151/155 (97%) Frame = +2 Query: 56 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 235 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 236 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 415 REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120 Query: 416 SGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 520 SGWHGDNMLE S+KMPWFKGW VERKEGK +GKCL Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCL 155 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 37.5 bits (83), Expect = 8e-05 Identities = 36/117 (30%), Positives = 52/117 (44%) Frame = +2 Query: 95 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 274 VT +D PGH FI G D VL+VAA G E QT + +A V Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246 Query: 275 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 445 +IV +NK+D + ++E K + I+++G + V IS G N+ E Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 1.9 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 80 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 181 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 2.5 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 80 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 181 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 5.8 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -2 Query: 457 FG*RLQHVVSVPSRNGHESDSSWV 386 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 5.8 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +3 Query: 300 WIPLNHHTVSPDLRKSRRKY 359 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 >AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor protein. Length = 1370 Score = 21.0 bits (42), Expect = 7.7 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = -2 Query: 241 LTGLTVLRDTSFEFTGTGSYDEHSAISLR 155 LTGLTVLR + + S E S R Sbjct: 257 LTGLTVLRTFNASYNSLDSLPEGLFASTR 285 >AB253415-1|BAE86926.1| 588|Apis mellifera alpha-glucosidase protein. Length = 588 Score = 21.0 bits (42), Expect = 7.7 Identities = 8/18 (44%), Positives = 12/18 (66%) Frame = -2 Query: 352 LLDFLKSGLTVWWFSGIH 299 L F++SG+T W S I+ Sbjct: 56 LSHFIESGITAIWLSPIN 73 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 157,713 Number of Sequences: 438 Number of extensions: 3179 Number of successful extensions: 13 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 13 length of database: 146,343 effective HSP length: 54 effective length of database: 122,691 effective search space used: 14600229 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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