SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS304C08f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g35680.2 68418.m04264 eukaryotic translation initiation facto...   163   6e-41
At5g35680.1 68418.m04263 eukaryotic translation initiation facto...   163   6e-41
At2g04520.1 68415.m00458 eukaryotic translation initiation facto...   162   1e-40
At2g40780.1 68415.m05031 hypothetical protein                          35   0.029
At1g17600.1 68414.m02173 disease resistance protein (TIR-NBS-LRR...    31   0.62 
At3g27785.1 68416.m03466 myb family transcription factor (MYB118...    29   1.4  
At4g38270.1 68417.m05406 glycosyl transferase family 8 protein c...    28   4.4  
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam...    28   4.4  
At5g40360.1 68418.m04896 myb family transcription factor (MYB115...    27   5.8  
At5g35180.1 68418.m04169 expressed protein                             27   7.7  
At2g07690.1 68415.m00993 minichromosome maintenance family prote...    27   7.7  

>At5g35680.2 68418.m04264 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  163 bits (396), Expect = 6e-41
 Identities = 74/105 (70%), Positives = 85/105 (80%)
 Frame = +3

Query: 132 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 311
           L+FKEDGQEYAQV +MLGNGR + MC DG+KRLCHIRGK+ KKVWI  GDIIL+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKKVWIAAGDIILVGLRDYQ 85

Query: 312 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDEDI 446
           D KADVILKY  DEAR LK YGE PE  R+NE +V  +D  D+D+
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLDD-DDDV 129


>At5g35680.1 68418.m04263 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  163 bits (396), Expect = 6e-41
 Identities = 74/105 (70%), Positives = 85/105 (80%)
 Frame = +3

Query: 132 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 311
           L+FKEDGQEYAQV +MLGNGR + MC DG+KRLCHIRGK+ KKVWI  GDIIL+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKKVWIAAGDIILVGLRDYQ 85

Query: 312 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDEDI 446
           D KADVILKY  DEAR LK YGE PE  R+NE +V  +D  D+D+
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLDD-DDDV 129


>At2g04520.1 68415.m00458 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  162 bits (393), Expect = 1e-40
 Identities = 73/104 (70%), Positives = 84/104 (80%)
 Frame = +3

Query: 132 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 311
           L+FKEDGQEYAQV +MLGNGR EAMC DG KRLCHIRGK+ KKVWI  GDI+L+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIVLVGLRDYQ 85

Query: 312 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDED 443
           D KADVILKY  DEAR LK YGE PE  R+NE +V  ++  D++
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLEDDDDN 129


>At2g40780.1 68415.m05031 hypothetical protein 
          Length = 171

 Score = 35.1 bits (77), Expect = 0.029
 Identities = 16/50 (32%), Positives = 26/50 (52%)
 Frame = +3

Query: 144 EDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILI 293
           E+ Q  AQV  + G+ ++E M   G   L     K R+ +WI +G  ++I
Sbjct: 20  EECQSIAQVVSLRGSNQIEIMDAKGENSLALFPAKFRESMWIRRGSFVVI 69


>At1g17600.1 68414.m02173 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1025

 Score = 30.7 bits (66), Expect = 0.62
 Identities = 13/47 (27%), Positives = 25/47 (53%)
 Frame = +3

Query: 165 QVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRD 305
           ++ K LGN ++  +  DG+ ++  +    ++K W   G  I+I  RD
Sbjct: 280 EIKKRLGNQKV-FLVLDGVDKVAQVHALAKEKNWFGPGSRIIITTRD 325


>At3g27785.1 68416.m03466 myb family transcription factor (MYB118)
           contains PFAM profile: PF00249 myb-like DNA binding
           domain
          Length = 437

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 16/47 (34%), Positives = 27/47 (57%)
 Frame = +3

Query: 153 QEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILI 293
           ++++Q+ KML  GR+   C +      H+R  ++K  W  + DIILI
Sbjct: 210 KKWSQIAKML-QGRVGKQCRERWHN--HLRPDIKKDGWTEEEDIILI 253


>At4g38270.1 68417.m05406 glycosyl transferase family 8 protein
           contains Pfam profile: PF01501 glycosyl transferase
           family 8
          Length = 680

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 13/48 (27%), Positives = 21/48 (43%)
 Frame = +3

Query: 300 RDYQDAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDED 443
           RDY+   AD ILK   D+    K Y    ++  +    V+ +    E+
Sbjct: 207 RDYESPNADAILKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGEN 254


>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
           protein similar to  PHO1 protein [Arabidopsis thaliana]
           GI:20069032; contains Pfam profiles PF03105: SPX domain,
           PF03124: EXS family
          Length = 756

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = -1

Query: 140 KDQLTFFSFIFVLSSPPIFSSFTFVFRHVL 51
           KD   F +F+F++S+ P FS F    R +L
Sbjct: 562 KDSQVFNTFLFIVSAFPFFSRFLQCMRRML 591


>At5g40360.1 68418.m04896 myb family transcription factor (MYB115)
           contains Pfam profile: PF00249 myb-like DNA-binding
           domain
          Length = 359

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 19/78 (24%), Positives = 33/78 (42%)
 Frame = +3

Query: 132 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 311
           +V  +  + +  + KM   GR+   C +      H+R  ++K  W  + D ILI +    
Sbjct: 172 MVKSKGTKNWTSIAKMF-QGRVGKQCRERWHN--HLRPNIKKNDWSEEEDQILIEVHKIV 228

Query: 312 DAKADVILKYTPDEARNL 365
             K   I K  P  + N+
Sbjct: 229 GNKWTEIAKRLPGRSENI 246


>At5g35180.1 68418.m04169 expressed protein
          Length = 778

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +1

Query: 109 KMKLKNVSWSLRKTDKSTPKSQRCS 183
           K+KLKNVSW++       PK+   S
Sbjct: 506 KVKLKNVSWAIASLSLKRPKAPGAS 530


>At2g07690.1 68415.m00993 minichromosome maintenance family protein
           / MCM family protein similar to SP|P55862 DNA
           replication licensing factor MCM5 (CDC46 homolog)
           {Xenopus laevis}; contains Pfam profile PF00493:
           MCM2/3/5 family
          Length = 727

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
 Frame = +3

Query: 261 VWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVR-INETVVYSVDGLD 437
           V I Q  I ++GL D  +A +     +TPDE    K + +  +  + I   +  S+ G  
Sbjct: 282 VAIRQPYIRVVGLEDTNEASSRGPANFTPDEEEEFKKFADSQDVYKNICTKIAPSIFG-H 340

Query: 438 EDIE 449
           ED++
Sbjct: 341 EDVK 344


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,223,902
Number of Sequences: 28952
Number of extensions: 167328
Number of successful extensions: 538
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 538
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -