BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS304A10f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 116 2e-27 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 116 2e-27 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 116 2e-27 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 6e-07 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.060 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 3.9 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 5.2 SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 6.8 SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 6.8 SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|... 25 9.0 SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 9.0 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 116 bits (279), Expect = 2e-27 Identities = 52/72 (72%), Positives = 62/72 (86%) Frame = +1 Query: 1 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180 CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427 Query: 181 RQTVAVGVIKAV 216 RQTVAVGVIKAV Sbjct: 428 RQTVAVGVIKAV 439 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 116 bits (279), Expect = 2e-27 Identities = 52/72 (72%), Positives = 62/72 (86%) Frame = +1 Query: 1 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180 CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427 Query: 181 RQTVAVGVIKAV 216 RQTVAVGVIKAV Sbjct: 428 RQTVAVGVIKAV 439 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 116 bits (279), Expect = 2e-27 Identities = 52/72 (72%), Positives = 62/72 (86%) Frame = +1 Query: 1 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180 CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427 Query: 181 RQTVAVGVIKAV 216 RQTVAVGVIKAV Sbjct: 428 RQTVAVGVIKAV 439 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 48.4 bits (110), Expect = 6e-07 Identities = 25/72 (34%), Positives = 42/72 (58%), Gaps = 1/72 (1%) Frame = +1 Query: 7 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 186 FA++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD Sbjct: 592 FAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGT 650 Query: 187 TVAVG-VIKAVN 219 TVAVG V+K ++ Sbjct: 651 TVAVGKVVKILD 662 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 31.9 bits (69), Expect = 0.060 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = +1 Query: 118 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 210 PLC+ +E P LGRF +R TVA G++K Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 25.8 bits (54), Expect = 3.9 Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%) Frame = -1 Query: 260 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 87 S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++ Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241 Query: 86 SPDLMDFGLTSVDLPVRRSTFSLIS 12 S L +TS PV S+ SL S Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 5.2 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -1 Query: 95 MAASPDLMDFGLTSVDLPVRRSTFS 21 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 >SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 25.0 bits (52), Expect = 6.8 Identities = 10/37 (27%), Positives = 19/37 (51%) Frame = -1 Query: 275 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 165 P + ++ T+PP S+ T + PT ++ + TA Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109 >SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -1 Query: 248 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 126 + L P +S + TP+AT S+ + P GG D+T Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284 >SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 885 Score = 24.6 bits (51), Expect = 9.0 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = +3 Query: 276 QEVARAVNSTIFHTTAILHSPKGVSKEKRATNSFL 380 + +A A+N +I TT + K + E+ ++ SFL Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403 >SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +1 Query: 19 KEKVDRRTGKSTEVNPKSIKSGDAAIVNL 105 +EKV +RT S +N + ++ D ++NL Sbjct: 39 EEKVTQRTASSDSLNTIASENNDENVINL 67 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,876,416 Number of Sequences: 5004 Number of extensions: 33363 Number of successful extensions: 100 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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