SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS304A10f
         (521 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   116   2e-27
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   116   2e-27
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   116   2e-27
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    48   6e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.060
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    26   3.9  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   5.2  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   6.8  
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    25   6.8  
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|...    25   9.0  
SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   9.0  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  116 bits (279), Expect = 2e-27
 Identities = 52/72 (72%), Positives = 62/72 (86%)
 Frame = +1

Query: 1   CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180
           CKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427

Query: 181 RQTVAVGVIKAV 216
           RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  116 bits (279), Expect = 2e-27
 Identities = 52/72 (72%), Positives = 62/72 (86%)
 Frame = +1

Query: 1   CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180
           CKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427

Query: 181 RQTVAVGVIKAV 216
           RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  116 bits (279), Expect = 2e-27
 Identities = 52/72 (72%), Positives = 62/72 (86%)
 Frame = +1

Query: 1   CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 180
           CKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427

Query: 181 RQTVAVGVIKAV 216
           RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 48.4 bits (110), Expect = 6e-07
 Identities = 25/72 (34%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = +1

Query: 7   FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 186
           FA++  K+D+ T + ++  P     G   I  L    P+C+E F+++  +GRF +RD   
Sbjct: 592 FAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGT 650

Query: 187 TVAVG-VIKAVN 219
           TVAVG V+K ++
Sbjct: 651 TVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.060
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +1

Query: 118 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 210
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 25.8 bits (54), Expect = 3.9
 Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
 Frame = -1

Query: 260 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 87
           S  +VTLPPPAS   ++  T T T  + S ++     G+ + +++        +  ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241

Query: 86  SPDLMDFGLTSVDLPVRRSTFSLIS 12
           S  L    +TS   PV  S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 5.2
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -1

Query: 95  MAASPDLMDFGLTSVDLPVRRSTFS 21
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 10/37 (27%), Positives = 19/37 (51%)
 Frame = -1

Query: 275 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 165
           P  + ++   T+PP  S+  T  + PT ++   + TA
Sbjct: 73  PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -1

Query: 248 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 126
           + L P +S  +    TP+AT    S+  + P GG    D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284


>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 885

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = +3

Query: 276 QEVARAVNSTIFHTTAILHSPKGVSKEKRATNSFL 380
           + +A A+N +I  TT +    K +  E+ ++ SFL
Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403


>SPBC405.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 143

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = +1

Query: 19  KEKVDRRTGKSTEVNPKSIKSGDAAIVNL 105
           +EKV +RT  S  +N  + ++ D  ++NL
Sbjct: 39  EEKVTQRTASSDSLNTIASENNDENVINL 67


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,876,416
Number of Sequences: 5004
Number of extensions: 33363
Number of successful extensions: 100
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -