Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS304A01f
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30330.1 68415.m03691 GCN5L1 family protein similar to GCN5-l... 80 6e-16
At2g17930.1 68415.m02076 FAT domain-containing protein / phospha... 33 0.13
At5g38570.1 68418.m04664 F-box family protein contains F-box dom... 31 0.39
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha... 28 2.8
At3g04980.1 68416.m00541 DNAJ heat shock N-terminal domain-conta... 28 2.8
At3g52570.1 68416.m05788 expressed protein contains Interpro ent... 28 3.7
At5g41780.1 68418.m05087 myosin heavy chain-related weak similar... 27 4.8
At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains we... 27 6.4
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 27 8.5
At4g32190.1 68417.m04581 centromeric protein-related low similar... 27 8.5
At2g45120.1 68415.m05615 zinc finger (C2H2 type) family protein ... 27 8.5
>At2g30330.1 68415.m03691 GCN5L1 family protein similar to GCN5-like
protein 1 (RT14 protein) (Swiss-Prot:P78537) [Homo
sapiens]
Length = 152
Score = 80.2 bits (189), Expect = 6e-16
Identities = 39/100 (39%), Positives = 58/100 (58%)
Frame = +3
Query: 96 EQKRKECITAANDLTQALVDHLNVGVAQAYLNQKKLDAEAKLLHQGAINFSKQTQQWLTL 275
E+ RKE I A LV +N GV + ++N+K++++E + L F KQT QWL +
Sbjct: 53 ERSRKEAIRHAARTADLLVKAVNGGVEECFVNEKRIESEIRNLAITVAKFGKQTDQWLAV 112
Query: 276 VENFSSALKEIGDVENWARSIENDMKIITDTLERAYEKAQ 395
+SA+KEIGD ENW +++E D K IT + +E Q
Sbjct: 113 THAVNSAVKEIGDFENWMKTMEFDCKKITAAIRNIHEDQQ 152
>At2g17930.1 68415.m02076 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF02259 FAT domain, PF00454
Phosphatidylinositol 3- and 4-kinase, PF02260: FATC
domain
Length = 3795
Score = 32.7 bits (71), Expect = 0.13
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +3
Query: 213 AKLLHQGAINFSKQTQQWLTLVENFSSALKEIGDVENWARSIENDMKIITDT 368
A+ + Q + S+ + T EN A++ G V +W R +N+MK++TDT
Sbjct: 1888 AQFVPQMVNSLSRLGLPYNTTAENRRLAIELAGLVVSWERQRQNEMKMVTDT 1939
>At5g38570.1 68418.m04664 F-box family protein contains F-box domain
Pfam:PF00646
Length = 379
Score = 31.1 bits (67), Expect = 0.39
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = -2
Query: 279 PQESTIAVFVWKNLLHLGVKVLLPHLTFSDLNRLEQHRHLNDLP 148
PQ+ IAV++ KN HL L +D+ RL+ + L P
Sbjct: 322 PQDRDIAVYILKNACHLKTATFLTDKRINDVRRLKMIKELRLSP 365
>At4g36080.1 68417.m05136 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF00454: Phosphatidylinositol 3-
and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
Length = 3839
Score = 28.3 bits (60), Expect = 2.8
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = +3
Query: 213 AKLLHQGAINFSKQTQQWLTLVENFSSALKEIGDVENWARSIENDMKIITD 365
A+ + Q + S+ + T EN A++ G V +W R +N+ K++TD
Sbjct: 1944 AQFVPQMVNSLSRLGLPYNTTAENRRLAIELAGLVVSWERQRQNESKMVTD 1994
>At3g04980.1 68416.m00541 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile PF00226
DnaJ domain
Length = 1165
Score = 28.3 bits (60), Expect = 2.8
Identities = 12/43 (27%), Positives = 25/43 (58%)
Frame = +3
Query: 261 QWLTLVENFSSALKEIGDVENWARSIENDMKIITDTLERAYEK 389
Q +T+ + SSA+K+I +++W ++ DT+++ Y K
Sbjct: 28 QMMTICDVHSSAIKKIKGLDDWYGVLQVQPYADADTIKKQYRK 70
>At3g52570.1 68416.m05788 expressed protein contains Interpro entry
IPR000379
Length = 335
Score = 27.9 bits (59), Expect = 3.7
Identities = 14/56 (25%), Positives = 25/56 (44%)
Frame = +3
Query: 237 INFSKQTQQWLTLVENFSSALKEIGDVENWARSIENDMKIITDTLERAYEKAQEKP 404
+ FS+ +W+ S LK GD E W +++ +++ E +Y E P
Sbjct: 213 LGFSRSLSEWI------GSNLKRSGDSETWTFNLDGAVQMFNSYRETSYWSLLENP 262
>At5g41780.1 68418.m05087 myosin heavy chain-related weak similarity
to M protein, serotype 5 precursor (SP:P02977)
{Streptococcus pyogenes} and to Myosin heavy chain,
non-muscle (SP:Q99323) (Zipper protein) (Myosin II)
{Drosophila melanogaster}
Length = 537
Score = 27.5 bits (58), Expect = 4.8
Identities = 30/115 (26%), Positives = 47/115 (40%), Gaps = 2/115 (1%)
Frame = +3
Query: 60 HQAKQAAKRVVQEQKRKECITAANDLTQALVDHLNVGVAQAYLNQKKLDAEAKLLHQGAI 239
H+ A+++ E K T N TQ + N V++ + K E L G
Sbjct: 343 HRRIHVAEQIHLESKSSYIKTRDN--TQTEENRGNRAVSETQFKKIKEMVEQGLA--GPE 398
Query: 240 NFSKQTQQWLTLVENFSSALKEIGDVENWARSIENDMKIITDTLERAYE--KAQE 398
K+ ++ L + KEI W + +N+MK +TLE E +AQE
Sbjct: 399 MAIKKLEESGELGNRVTRLAKEIDSARKWVKEKDNNMKHEVETLEAKLECREAQE 453
>At5g04990.1 68418.m00528 sad1/unc-84 protein-related contains weak
similarity to Sad1/unc-84 protein-like 1
(Swiss-Prot:O94901) [Homo sapiens]
Length = 471
Score = 27.1 bits (57), Expect = 6.4
Identities = 24/94 (25%), Positives = 43/94 (45%)
Frame = +3
Query: 117 ITAANDLTQALVDHLNVGVAQAYLNQKKLDAEAKLLHQGAINFSKQTQQWLTLVENFSSA 296
I + L +A + + V Q L KK++ EAK+L Q + Q L +E+ + +
Sbjct: 158 IAEVDGLVKATTNSMQV---QVELLDKKMEREAKVLRQEIERKASAFQSELKKIESRTES 214
Query: 297 LKEIGDVENWARSIENDMKIITDTLERAYEKAQE 398
L++ D + + D LER YE+ ++
Sbjct: 215 LEKSVD------EVNAKPWVTKDELERIYEELKK 242
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 26.6 bits (56), Expect = 8.5
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +2
Query: 305 NWGCRKLGTKYRK*YENYYRHFRKSI 382
++ CRK YRK Y+ +Y R S+
Sbjct: 161 DYDCRKPAASYRKVYDYFYEKARASV 186
>At4g32190.1 68417.m04581 centromeric protein-related low similarity
to SP|Q02224 Centromeric protein E (CENP-E protein)
{Homo sapiens}
Length = 783
Score = 26.6 bits (56), Expect = 8.5
Identities = 16/61 (26%), Positives = 27/61 (44%), Gaps = 4/61 (6%)
Frame = +3
Query: 195 KKLDAEAKLLHQGAINFSKQTQQWLTLVENFS----SALKEIGDVENWARSIENDMKIIT 362
KKL+ E L + N KQT++WL + + +K +G+ K++T
Sbjct: 275 KKLEQEK--LRETEANLKKQTEEWLIAQDEVNKLKEETVKRLGEANETMEDFMKVKKLLT 332
Query: 363 D 365
D
Sbjct: 333 D 333
>At2g45120.1 68415.m05615 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 314
Score = 26.6 bits (56), Expect = 8.5
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +2
Query: 143 SIGRSFKCRCCSSLFKSEKVRCGSKTFTPRCNKFFQTNTAMVDS 274
S GR FKC C +FKS + G + + + NK T T V++
Sbjct: 190 SRGR-FKCETCGKVFKSYQALGGHRA-SHKKNKACMTKTEQVET 231
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,581,801
Number of Sequences: 28952
Number of extensions: 149841
Number of successful extensions: 529
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 519
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 529
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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