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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS303H12f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g04080.1 68417.m00577 iron-sulfur cluster assembly complex pr...   218   2e-57
At4g22220.1 68417.m03214 iron-sulfur cluster assembly complex pr...   217   5e-57
At3g01020.1 68416.m00003 iron-sulfur cluster assembly complex pr...   210   6e-55
At3g50100.1 68416.m05477 exonuclease family protein contains exo...    28   3.3  
At4g14080.1 68417.m02173 glycosyl hydrolase family 17 protein / ...    28   4.4  

>At4g04080.1 68417.m00577 iron-sulfur cluster assembly complex
           protein, putative similar to ISCU2 (GI:11545707) [Homo
           sapiens]
          Length = 171

 Score =  218 bits (532), Expect = 2e-57
 Identities = 99/138 (71%), Positives = 117/138 (84%)
 Frame = +1

Query: 106 SPNVILAAPYHANVIDHYENPRNVGSLDKKDKNVGTGLVGAPACGDVMKLQIKVDENGKI 285
           +P  +++  YH NVIDHY+NPRNVGS DK D NVGTGLVGAP CGDVMKLQ+K D +G+I
Sbjct: 19  TPFPVVSRLYHPNVIDHYDNPRNVGSFDKNDPNVGTGLVGAPQCGDVMKLQVKFDGSGQI 78

Query: 286 VDAKFKTFGCGSAIASSSLATEWVKGKTVDEALKLKNTDIAKELSLPPVKLHCSMLAEDA 465
           +DAKFKTFGCGSAIA+SS+ATEWVKGK+V+E L +KN+ IAK LSLPPVKLHCSMLAEDA
Sbjct: 79  IDAKFKTFGCGSAIAASSVATEWVKGKSVEEVLTIKNSQIAKHLSLPPVKLHCSMLAEDA 138

Query: 466 IKAALSDYRIKQQTENKE 519
           IKAA+ +Y+ KQ   N E
Sbjct: 139 IKAAIKNYKEKQDKANGE 156


>At4g22220.1 68417.m03214 iron-sulfur cluster assembly complex
           protein, putative similar to iron-sulfur cluster
           assembly complex ISCU1 (GI:11545705) [Homo sapiens];
           nifU protein homolog YPL135w (GI:15619823)
           [Saccharomyces cerevisiae] PIR2:S69049
          Length = 167

 Score =  217 bits (529), Expect = 5e-57
 Identities = 101/128 (78%), Positives = 113/128 (88%), Gaps = 1/128 (0%)
 Frame = +1

Query: 133 YHANVIDHYENPRNVGSLDKKDKNVGTGLVGAPACGDVMKLQIKVDEN-GKIVDAKFKTF 309
           YH NVIDHY+NPRNVGS DK D NVGTGLVGAPACGDVMKLQIKVDE  G+IVDA+FKTF
Sbjct: 29  YHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTF 88

Query: 310 GCGSAIASSSLATEWVKGKTVDEALKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDY 489
           GCGSAIASSS+ATEWVKGK +++ L +KNT+IAK LSLPPVKLHCSMLAEDAIKAA+ DY
Sbjct: 89  GCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDY 148

Query: 490 RIKQQTEN 513
           + K+   N
Sbjct: 149 KEKRVKTN 156


>At3g01020.1 68416.m00003 iron-sulfur cluster assembly complex
           protein, putative similar to ISCU2 (GI:11545707) [Homo
           sapiens]; similar to NIFU-like protein (GI:15919270)
           [Cowdria ruminantium]
          Length = 163

 Score =  210 bits (512), Expect = 6e-55
 Identities = 92/127 (72%), Positives = 111/127 (87%)
 Frame = +1

Query: 133 YHANVIDHYENPRNVGSLDKKDKNVGTGLVGAPACGDVMKLQIKVDENGKIVDAKFKTFG 312
           YH NVIDH+ENPRNVGS ++ D NVGTGLVGAPACGD+M LQIKVD++G+I+D +FKTFG
Sbjct: 27  YHENVIDHFENPRNVGSFNRNDPNVGTGLVGAPACGDLMSLQIKVDDSGQIIDTRFKTFG 86

Query: 313 CGSAIASSSLATEWVKGKTVDEALKLKNTDIAKELSLPPVKLHCSMLAEDAIKAALSDYR 492
           CGSAIASSS+A+EW+KGKT+DE + +KN +IAK L LPPVKLHCSMLAEDAIK+A+ DY+
Sbjct: 87  CGSAIASSSVASEWIKGKTLDEVMTIKNAEIAKHLRLPPVKLHCSMLAEDAIKSAVRDYK 146

Query: 493 IKQQTEN 513
            KQ   N
Sbjct: 147 EKQAKTN 153


>At3g50100.1 68416.m05477 exonuclease family protein contains
           exonuclease domain, Pfam:PF00929
          Length = 406

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 14/41 (34%), Positives = 26/41 (63%)
 Frame = -1

Query: 386 FSASSTVFPLTHSVARELEAIAEPHPKVLNLASTIFPFSST 264
           F ++ T+  + HS+ R+LE +   HPKV++ A  +F + +T
Sbjct: 213 FLSTGTIL-VGHSLNRDLEVLKIDHPKVIDTA-LVFKYPNT 251


>At4g14080.1 68417.m02173 glycosyl hydrolase family 17 protein /
           anther-specific protein (A6) identical to probable
           glucan endo-1,3-beta-glucosidase A6 precursor SP:Q06915
           from [Arabidopsis thaliana]
          Length = 478

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
 Frame = -1

Query: 326 IAEPHPKVLNLASTIFPFSSTLICNFITSPHAGAPTRP---VPTFLSFLSNE 180
           I E    +LN A+    ++  LI     SP  G P+RP   +PTF+  L NE
Sbjct: 289 IDETGANILNAAT----YNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFNE 336


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,858,863
Number of Sequences: 28952
Number of extensions: 222294
Number of successful extensions: 633
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 628
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 632
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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