BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS303H08f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 34 0.011 SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 31 0.079 SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.32 SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1... 29 0.56 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 27 1.3 SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 27 1.3 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 3.9 SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe... 25 5.2 SPBC26H8.02c |sec9||SNAP-25 homologue, t-SNARE component Sec9|Sc... 25 6.8 SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 25 6.8 SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 6.8 SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosa... 25 9.0 SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces ... 25 9.0 SPBC887.15c |||sphingosine hydroxylase |Schizosaccharomyces pomb... 25 9.0 >SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 658 Score = 34.3 bits (75), Expect = 0.011 Identities = 15/32 (46%), Positives = 21/32 (65%) Frame = +3 Query: 300 QNFNANSNSQQYGSESGDNYPTNNSISGNENS 395 QN+N N+N+Q YG+ + NY NN+ S NS Sbjct: 611 QNYN-NNNNQNYGNNNNQNYNNNNNSSQGGNS 641 Score = 33.9 bits (74), Expect = 0.015 Identities = 21/69 (30%), Positives = 33/69 (47%), Gaps = 10/69 (14%) Frame = +3 Query: 231 SNMGNFHNSNFSDIGYPQ------MGGYDQNF----NANSNSQQYGSESGDNYPTNNSIS 380 SN GN++N+N + + G +F + NSN Q Y + + NY NN+ + Sbjct: 569 SNSGNYNNNNSGNNNQQHQQSSSSLQGLASSFLNSSSGNSNKQNYNNNNNQNYGNNNNQN 628 Query: 381 GNENSYDSQ 407 N N+ SQ Sbjct: 629 YNNNNNSSQ 637 Score = 32.3 bits (70), Expect = 0.045 Identities = 27/114 (23%), Positives = 46/114 (40%) Frame = +3 Query: 9 SPTSSYQDYQNALISQHANAISHLTQQTKSTFENYQSQHQAFVGHTMQQIQQLEMQKHAL 188 S TS+ + + L S A S L + N + ++ QQ + +L Sbjct: 534 SNTSNNESMVSKLSSLSGMASSFLGSSGNNNNNNNSNSGNYNNNNSGNNNQQHQQSSSSL 593 Query: 189 EMQKTXXXXXXXXXSNMGNFHNSNFSDIGYPQMGGYDQNFNANSNSQQYGSESG 350 + + SN N++N+N + G +QN+N N+NS Q G+ G Sbjct: 594 QGLASSFLNSSSGNSNKQNYNNNNNQNYG----NNNNQNYNNNNNSSQGGNSQG 643 Score = 26.6 bits (56), Expect = 2.2 Identities = 23/81 (28%), Positives = 39/81 (48%), Gaps = 5/81 (6%) Frame = +3 Query: 240 GNFHNSNFSDIGYPQMGGYDQNFNANSNSQ-QYGSESGDNYPTN--NSISGNEN--SYDS 404 GN +N+N S+ G Y+ N + N+N Q Q S S ++ NS SGN N +Y++ Sbjct: 561 GNNNNNNNSN-----SGNYNNNNSGNNNQQHQQSSSSLQGLASSFLNSSSGNSNKQNYNN 615 Query: 405 QIFEQIAKQKKENIEDPDLSN 467 + +N + + S+ Sbjct: 616 NNNQNYGNNNNQNYNNNNNSS 636 Score = 26.2 bits (55), Expect = 3.0 Identities = 19/73 (26%), Positives = 34/73 (46%) Frame = +3 Query: 231 SNMGNFHNSNFSDIGYPQMGGYDQNFNANSNSQQYGSESGDNYPTNNSISGNENSYDSQI 410 +N N N+N + M + + ++S + SG+N NNS SGN N+ +S Sbjct: 524 NNNSNTSNNNSNTSNNESMVSKLSSLSGMASS--FLGSSGNNNNNNNSNSGNYNNNNSGN 581 Query: 411 FEQIAKQKKENIE 449 Q +Q +++ Sbjct: 582 NNQQHQQSSSSLQ 594 >SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Spt6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1365 Score = 31.5 bits (68), Expect = 0.079 Identities = 20/71 (28%), Positives = 34/71 (47%), Gaps = 2/71 (2%) Frame = +3 Query: 252 NSNFSDIGYPQMGGYDQNFNANSNSQQYGSESGDNYPTNNSISGNENSYDSQIFEQIAKQ 431 N + ++ GYP G N + N+N E D P +N N++S +S E+ +Q Sbjct: 13 NGDKNEDGYPAENGEGTNVDDNNN-----EEEKDGIPLDNDNDENDSSEESATDEEAERQ 67 Query: 432 KKEN--IEDPD 458 +E +ED + Sbjct: 68 VREGFIVEDEE 78 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 29.5 bits (63), Expect = 0.32 Identities = 27/98 (27%), Positives = 41/98 (41%), Gaps = 3/98 (3%) Frame = +3 Query: 231 SNMGNFHNSNFSDIGYPQMGGYDQNFNANSNSQQYGSESGDNYPTNNSISGNENSYDSQ- 407 ++ N NSN Y Q+G N + N+ + ES Y SIS NE + ++ Sbjct: 32 TSSSNSENSNIQGKHYTQVGEDADNSFISENTPKNTFESTQTYENLESISKNEPTSEASK 91 Query: 408 -IFEQIAKQKKENIEDPDLSNLPNVNFSVPP-PGFKPL 515 + ++ ++ E P LPN P PG PL Sbjct: 92 PLLNELVPEEPLPREPP----LPNEPVPEEPLPGEPPL 125 >SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 28.7 bits (61), Expect = 0.56 Identities = 20/108 (18%), Positives = 45/108 (41%) Frame = +3 Query: 51 SQHANAISHLTQQTKSTFENYQSQHQAFVGHTMQQIQQLEMQKHALEMQKTXXXXXXXXX 230 S +++ + ++KS ++Q+ H + + L + +++ Sbjct: 619 SVRSSSYNSTASESKSEITSHQNLHTIPINKPFTSDRPLYSSPNDT-LERVETGNQGQRM 677 Query: 231 SNMGNFHNSNFSDIGYPQMGGYDQNFNANSNSQQYGSESGDNYPTNNS 374 +++GN + + DI + G +D N +N S G N P+N+S Sbjct: 678 NSIGNASSFSKRDIMENENGSFDTNAKNGNNVDDSSSVRGMNLPSNSS 725 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 27.5 bits (58), Expect = 1.3 Identities = 24/75 (32%), Positives = 31/75 (41%), Gaps = 6/75 (8%) Frame = +3 Query: 312 ANSNSQQYGSESG------DNYPTNNSISGNENSYDSQIFEQIAKQKKENIEDPDLSNLP 473 A+S QY +E G D Y + S N D+ F + + N DP +L Sbjct: 85 ASSGINQYSTEKGKFTRPSDEYESEYS-DYNAQPSDANNFYNLRGDGRYNAYDPSSDSLA 143 Query: 474 NVNFSVPPPGFKPLD 518 NV SV P G P D Sbjct: 144 NVYNSV-PYGSSPYD 157 >SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 619 Score = 27.5 bits (58), Expect = 1.3 Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 4/45 (8%) Frame = +3 Query: 342 ESGDN--YPTNNSISGNENSY--DSQIFEQIAKQKKENIEDPDLS 464 ESG+N +P N S Y D+QI EQI K + + PDLS Sbjct: 89 ESGENSDHPLNTSDDSKWKEYFDDNQILEQIDKDIRRTL--PDLS 131 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.8 bits (54), Expect = 3.9 Identities = 12/51 (23%), Positives = 23/51 (45%) Frame = +3 Query: 48 ISQHANAISHLTQQTKSTFENYQSQHQAFVGHTMQQIQQLEMQKHALEMQK 200 I + + HL+ + K + QH+ F Q++ QL K ++ Q+ Sbjct: 1697 IRRRDDRCEHLSGKLKKLHSQLEEQHETFFRAEQQRMTQLGFLKETVKKQE 1747 >SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 405 Score = 25.4 bits (53), Expect = 5.2 Identities = 21/70 (30%), Positives = 29/70 (41%) Frame = +3 Query: 297 DQNFNANSNSQQYGSESGDNYPTNNSISGNENSYDSQIFEQIAKQKKENIEDPDLSNLPN 476 D+N N Q YG + N +NN + N Y I +I EN+ +LS P+ Sbjct: 322 DENLPYNDYLQYYGPDYKLNVLSNNMENHNTRQYLDSITSEII----ENLR--NLSFAPS 375 Query: 477 VNFSVPPPGF 506 V P F Sbjct: 376 VQMHKTPGDF 385 >SPBC26H8.02c |sec9||SNAP-25 homologue, t-SNARE component Sec9|Schizosaccharomyces pombe|chr 2|||Manual Length = 419 Score = 25.0 bits (52), Expect = 6.8 Identities = 19/63 (30%), Positives = 26/63 (41%), Gaps = 5/63 (7%) Frame = +3 Query: 231 SNMGNFHNSNFSDIGYPQMGGYDQNF---NANSNSQQYGSESGDNYPTNNSIS--GNENS 395 SN N+ +G Y N N NSNS S + NY ++N+ G N+ Sbjct: 22 SNSNTATNAPSYSVGGTTANSYSSNSYNDNNNSNSTYGSSNNYGNYGSSNNYGSYGASNT 81 Query: 396 YDS 404 Y S Sbjct: 82 YGS 84 >SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 25.0 bits (52), Expect = 6.8 Identities = 9/35 (25%), Positives = 17/35 (48%) Frame = +3 Query: 354 NYPTNNSISGNENSYDSQIFEQIAKQKKENIEDPD 458 ++P ++ GN Y I + + +KEN+ D Sbjct: 44 SFPIKPNVKGNNEKYFKSITSDLYEWQKENLNKED 78 >SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1|||Manual Length = 881 Score = 25.0 bits (52), Expect = 6.8 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = -3 Query: 210 FLEFFASQGHVFAFPIVVFVA 148 F FF SQGH F + +F A Sbjct: 423 FFGFFGSQGHWFTSSVFLFTA 443 >SPCC553.09c |spb70|pol12|DNA polymerase alpha B-subunit|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 24.6 bits (51), Expect = 9.0 Identities = 13/50 (26%), Positives = 22/50 (44%) Frame = +1 Query: 13 PPAPTKIIRTP*SHSMRMLFRI*HSKLNLLLRIINHNTKLLLVTQCNKYN 162 P AP+K++ +RI + KL +++ +L C KYN Sbjct: 139 PSAPSKLVIVANFDLKAFNYRIMYQKLYDSSEVLDDRIELFSALTCRKYN 188 >SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 24.6 bits (51), Expect = 9.0 Identities = 13/48 (27%), Positives = 21/48 (43%) Frame = +3 Query: 252 NSNFSDIGYPQMGGYDQNFNANSNSQQYGSESGDNYPTNNSISGNENS 395 + +F GY Q+GG + + ++ S + NS S NE S Sbjct: 86 SDSFPTFGYSQLGGLENSSKGSALFNSANSIGTPYLSSRNSNSANEAS 133 >SPBC887.15c |||sphingosine hydroxylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 293 Score = 24.6 bits (51), Expect = 9.0 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = -3 Query: 447 QYFLSFALLFVQIFGYHNYSHFH*YCCLLGNYPHFHYH 334 QYF +F ++ + +H Y H Y L N H H+H Sbjct: 133 QYFFAFFIIDSWQYFWHRYLH---YNKKLYNMIHAHHH 167 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,736,066 Number of Sequences: 5004 Number of extensions: 30508 Number of successful extensions: 155 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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