BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS303G09f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo... 47 1e-06 SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|... 28 0.73 SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharo... 28 0.97 SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo... 27 1.7 SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc... 27 2.2 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 3.0 SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 3.0 SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 26 3.9 SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces... 25 5.2 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 5.2 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 25 5.2 SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharom... 25 6.8 SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 25 6.8 SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces p... 25 9.0 SPAC56F8.15 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 9.0 SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce... 25 9.0 >SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 47.2 bits (107), Expect = 1e-06 Identities = 26/113 (23%), Positives = 61/113 (53%), Gaps = 1/113 (0%) Frame = +2 Query: 143 DKKGQGIAKNFAESLEYGEKKKWFH-QLPEEPVTLPKTLSTEQIEQLRKEATSSLHGDTL 319 +++G+G + + + W++ LPE K ++ + + +L L D+ Sbjct: 17 NEEGEGTIDTKPQGVMFDPGVPWYNIPLPELHED-KKFVNVDHVSELETRGLLLLKEDSE 75 Query: 320 AYETKTNRSGSSDHQWAKTLLNKGAIGDRVAAATILIQDNPLYNLTALRNLIN 478 + ++T G++D + +TL++ G DR++A T+L+Q++P++ + AL L++ Sbjct: 76 RF-SETLGHGTADKRMLQTLISSGTTSDRISALTLLVQESPIHAVKALETLLS 127 >SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|chr 3|||Manual Length = 700 Score = 28.3 bits (60), Expect = 0.73 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = -1 Query: 227 QVADETISFFHHIQGFRRNSLRYPGLSYHLRSYQLDRN 114 QVA++ FFH+ G R+ + SYH +Y +D N Sbjct: 632 QVAEKVKRFFHYY-GINRHKMTTLTPSYHAETYGVDDN 668 >SPBC1539.10 |||ribosome biogenesis protein Nop16 |Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 27.9 bits (59), Expect = 0.97 Identities = 26/102 (25%), Positives = 43/102 (42%) Frame = +2 Query: 191 YGEKKKWFHQLPEEPVTLPKTLSTEQIEQLRKEATSSLHGDTLAYETKTNRSGSSDHQWA 370 +GE K L +E P TE + QL +EA K + +H + Sbjct: 114 HGEAKTLDDVLDKEISIAPA--KTEVVRQLEEEAVKK----AARQSNKMLPLSAFEHAYI 167 Query: 371 KTLLNKGAIGDRVAAATILIQDNPLYNLTALRNLINNVKPAK 496 + L+NK D + A + ++ L+N + L+NL +K K Sbjct: 168 QRLINKYGTEDFESMAKDVKLNSKLFNGSKLKNLYIRMKATK 209 >SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizosaccharomyces pombe|chr 3|||Manual Length = 569 Score = 27.1 bits (57), Expect = 1.7 Identities = 7/14 (50%), Positives = 12/14 (85%) Frame = -1 Query: 308 RARNWWLPFLIVQF 267 R+ WWLP+++V+F Sbjct: 113 RSHAWWLPYIVVEF 126 >SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 26.6 bits (56), Expect = 2.2 Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 1/66 (1%) Frame = +2 Query: 206 KWFHQLPEEPVTLPKTLSTEQIEQLRKEATSSLHGDTLAYETKTNR-SGSSDHQWAKTLL 382 K HQ+ E + STE +E L S+LH + K + G +++ W T + Sbjct: 546 KPIHQIGAEASSSNNVKSTELLEGLANARKSALHEIFVKINLKIDDFLGLAEYDWTTTQV 605 Query: 383 NKGAIG 400 K G Sbjct: 606 RKDVSG 611 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 26.2 bits (55), Expect = 3.0 Identities = 12/38 (31%), Positives = 18/38 (47%) Frame = +3 Query: 141 MIRKARVSQRISPKALNMVKKRNGFISYLKNQSHCQKH 254 ++ S R LNM KKR I +++ H QK+ Sbjct: 174 LVYAVEFSHRSGRDLLNMAKKRTNVIPIVEDARHVQKY 211 >SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 26.2 bits (55), Expect = 3.0 Identities = 15/42 (35%), Positives = 23/42 (54%) Frame = -2 Query: 133 LISLTVISVSLIN*YVFLKSFMLHCKLLYHVTLNINKARENL 8 LIS V L++ +K +++ +L Y + L I ARENL Sbjct: 829 LISSLRSKVDLLDRKFLIKEYLVLQQLKYSINLLITAARENL 870 >SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizosaccharomyces pombe|chr 1|||Manual Length = 1604 Score = 25.8 bits (54), Expect = 3.9 Identities = 22/109 (20%), Positives = 44/109 (40%) Frame = +2 Query: 65 KHKRFEENILIDEAYTNYGQADKNADDKKGQGIAKNFAESLEYGEKKKWFHQLPEEPVTL 244 K K E+ + +A ++ +A ++D K N SL+ PE P+T Sbjct: 471 KAKVKAESKVRSKAKSDKSRAKLSSDTNKDSEKNDNNDASLQSAGVASDGESSPETPLTK 530 Query: 245 PKTLSTEQIEQLRKEATSSLHGDTLAYETKTNRSGSSDHQWAKTLLNKG 391 + +L + + + +G+T + K+ + S Q A + +G Sbjct: 531 ASKSKKAKASKLANDTSKNANGETKSTPKKSKKKTSKAQQEANSTTAEG 579 >SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1441 Score = 25.4 bits (53), Expect = 5.2 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = +2 Query: 347 GSSDHQWAKTLLNKGAIGDRVAAATILIQDNPLYNLTA 460 G ++ + TL +KG V+AA L+Q LY + A Sbjct: 1235 GFAEEPYRMTLFSKGQEALNVSAADFLVQGENLYFVVA 1272 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 25.4 bits (53), Expect = 5.2 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +2 Query: 278 LRKEATSSLHGDTLAYETKTNRSGS 352 LR+ + LHG LAY+ +SGS Sbjct: 222 LRQSSVEYLHGSLLAYKELFEKSGS 246 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 25.4 bits (53), Expect = 5.2 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = +2 Query: 164 AKNFAESLEYGEKKKWFHQLPEEPVTLPKTLSTEQIEQLRKE 289 A+N A E K HQL E + + ++ EQ EQL+ E Sbjct: 1451 AENEAMQKEIESLKDSNHQLQESASSDAEQITKEQFEQLKSE 1492 >SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharomyces pombe|chr 3|||Manual Length = 595 Score = 25.0 bits (52), Expect = 6.8 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +3 Query: 108 TLITVKLIRTQMIRKARVSQRISPKALNMVKKRNGFISYLKN 233 T+ +V+L TQ + V + + PK + V K GF+ + KN Sbjct: 529 TVSSVRLGHTQRHLR-HVPEYLLPKGMQAVNKDIGFVPFKKN 569 >SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.0 bits (52), Expect = 6.8 Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 1/56 (1%) Frame = -2 Query: 205 LFFTIFKAFGEILCDTLAFLI-ICVLISLTVISVSLIN*YVFLKSFMLHCKLLYHV 41 LF+ F A G LC T+ L +C SL + + Y ++ +L L YH+ Sbjct: 270 LFYARFSAVGHNLCPTITELCKLCSKESLDAFLPAFYDVYFQCRTRLLKPVLDYHL 325 >SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/26 (38%), Positives = 15/26 (57%) Frame = +3 Query: 147 RKARVSQRISPKALNMVKKRNGFISY 224 R A + + PKA ++K +NG I Y Sbjct: 139 RDAEIVKAAIPKATEVLKSKNGSIDY 164 >SPAC56F8.15 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 176 Score = 24.6 bits (51), Expect = 9.0 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = -1 Query: 287 PFLIVQFVLSIMFLAM*LVLQVADETISFFHH 192 PF + LS L + L + T+ FFHH Sbjct: 34 PFYSILCFLSFFALLLHLPCSIYSHTLHFFHH 65 >SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces pombe|chr 2|||Manual Length = 406 Score = 24.6 bits (51), Expect = 9.0 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 380 LNKGAIGDRVAAATILIQDNPLYNLTALRNLINN 481 LN+G + A + + +P+YN A NL NN Sbjct: 92 LNEGRHSPKSAISCVTQPVSPVYNTRAAANLRNN 125 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,104,972 Number of Sequences: 5004 Number of extensions: 41885 Number of successful extensions: 145 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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