Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS303F12f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49510.1 68418.m06127 VHL binding protein, putative / prefold... 80 1e-15
At5g62250.1 68418.m07816 microtubule associated protein (MAP65/A... 34 0.050
At1g68790.1 68414.m07863 expressed protein 33 0.15
At3g53260.1 68416.m05870 phenylalanine ammonia-lyase 2 (PAL2) ne... 31 0.62
At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1) ne... 30 1.1
At1g75150.1 68414.m08729 expressed protein ; expression supporte... 29 1.4
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 29 1.4
At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to ... 29 1.4
At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative s... 29 1.9
At3g47060.1 68416.m05110 FtsH protease, putative contains simila... 29 2.5
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p... 28 4.4
At1g10950.1 68414.m01257 endomembrane protein 70, putative 28 4.4
At3g19720.2 68416.m02498 dynamin family protein identical to cDN... 27 5.8
At3g19720.1 68416.m02497 dynamin family protein identical to cDN... 27 5.8
At1g30190.1 68414.m03690 expressed protein ; expression supporte... 27 5.8
At4g11420.1 68417.m01840 eukaryotic translation initiation facto... 27 7.7
At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase fa... 27 7.7
At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase fa... 27 7.7
At3g13950.1 68416.m01761 expressed protein 27 7.7
At1g47900.1 68414.m05334 expressed protein 27 7.7
>At5g49510.1 68418.m06127 VHL binding protein, putative / prefoldin,
putative similar to Swiss-Prot:Q15765 prefoldin subunit
3 (Von Hippel-Lindau binding protein 1; VHL binding
protein-1; VBP-1; HIBBJ46) [Mus musculus]
Length = 195
Score = 79.8 bits (188), Expect = 1e-15
Identities = 44/116 (37%), Positives = 72/116 (62%), Gaps = 5/116 (4%)
Frame = +1
Query: 184 GIPEAEFVDNVDEFMKSPINAEGVDV--VLKSLDEKHRKYKVMEYTLATKRRRLRQQIPD 357
GIP A+F+ +V+ ++ G+D L E+ ++YKV+E L ++R L+ +IPD
Sbjct: 18 GIPAAKFIQDVETYLSQ----SGLDPNSALAFHQERLQQYKVVEMKLLAQQRDLQAKIPD 73
Query: 358 LARTIEVIEKLKEQK---EEVETQFLLSDQVFVKANVPPTKSVCLWLGANVMLEYS 516
+ + +EV+ L+ +K E + F +S+ ++ +A + T SVCLWLGANVMLEYS
Sbjct: 74 IEKCLEVVATLEAKKGTGEALLADFEVSEGIYSRACIEDTDSVCLWLGANVMLEYS 129
>At5g62250.1 68418.m07816 microtubule associated protein
(MAP65/ASE1) family protein low similarity to protein
regulating cytokinesis 1 (PRC1) [Homo sapiens]
GI:2865521; contains Pfam profile PF03999: Microtubule
associated protein (MAP65/ASE1 family)
Length = 549
Score = 34.3 bits (75), Expect = 0.050
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = +1
Query: 217 DEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIE 375
DE S +A+ D+ L+ L+E HR+ YTL ++R +QI D RT+E
Sbjct: 145 DETCSSDFSADESDLSLRKLEELHREL----YTLQEQKRNRVKQIQDNIRTLE 193
>At1g68790.1 68414.m07863 expressed protein
Length = 1085
Score = 32.7 bits (71), Expect = 0.15
Identities = 18/58 (31%), Positives = 29/58 (50%)
Frame = +1
Query: 247 EGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVETQ 420
EGV K LD + + K E L + ++L + L E + KLK++ EE+ T+
Sbjct: 415 EGVKKKEKDLDARLKTVKEKEKALKAEEKKLHMENERLLEDKECLRKLKDEIEEIGTE 472
>At3g53260.1 68416.m05870 phenylalanine ammonia-lyase 2 (PAL2)
nearly identical to SP|P45724
Length = 717
Score = 30.7 bits (66), Expect = 0.62
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = -1
Query: 248 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 99
S L+ DF N+ S +AS P D GF+G+ ++ YC +Q+L N
Sbjct: 429 SELVNDFYNNGLPSNLTASSNPSLDYGFKGA--EIAMASYCS-ELQYLAN 475
>At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1)
nearly identical to SP|P35510
Length = 725
Score = 29.9 bits (64), Expect = 1.1
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = -1
Query: 248 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 99
S L+ DF N+ S +AS P D GF+G+ ++ YC +Q+L N
Sbjct: 437 SELVNDFYNNGLPSNLTASRNPSLDYGFKGA--EIAMASYCS-ELQYLAN 483
>At1g75150.1 68414.m08729 expressed protein ; expression supported
by MPSS
Length = 753
Score = 29.5 bits (63), Expect = 1.4
Identities = 18/72 (25%), Positives = 34/72 (47%), Gaps = 2/72 (2%)
Frame = +1
Query: 217 DEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLART--IEVIEKL 390
D+F + P++ +++ K E + + L + R + L R V+EK+
Sbjct: 197 DDFDELPVSTASMNMTKKERREYLDQLRAENQRLLRETRDAAFEAAPLVRKPISSVLEKI 256
Query: 391 KEQKEEVETQFL 426
+ +KEE+ QFL
Sbjct: 257 RRRKEEISKQFL 268
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 29.5 bits (63), Expect = 1.4
Identities = 22/86 (25%), Positives = 40/86 (46%)
Frame = +1
Query: 199 EFVDNVDEFMKSPINAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEV 378
E DN++E M+ +E + D+K + V ++ A L Q+I DL IE+
Sbjct: 424 EGADNIEESMRRSCRSETDE---DDHDQKALEDLVKKHVDAKDTHILEQKITDLYNEIEI 480
Query: 379 IEKLKEQKEEVETQFLLSDQVFVKAN 456
++ K++ E Q L ++ + N
Sbjct: 481 YKRDKDELEIQMEQLALDYEILKQQN 506
>At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to
syntaxin-related protein Nt-syr1 GI:4206787 from
[Nicotiana tabacum]
Length = 303
Score = 29.5 bits (63), Expect = 1.4
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +1
Query: 241 NAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVE 414
N +GV+ + KSL + + + K + K R + D+A+ ++ ++ +K++ E +E
Sbjct: 46 NMKGVETLYKSLQDSNEECKTVHNAKKVKELRAKMD-GDVAQVLKRVKMIKQKLEALE 102
>At3g10340.1 68416.m01240 phenylalanine ammonia-lyase, putative
similar to phenylalanine ammonia-lyase GB:S48726
[Petroselinum crispum]
Length = 707
Score = 29.1 bits (62), Expect = 1.9
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = -1
Query: 248 SALIGDFINSSTLSTNSASGIPEYDLGFEGSTPSPSIFIYCEVYIQFLEN 99
S L+ DF N+ S S P D GF+G+ ++ YC +QFL N
Sbjct: 419 SELVNDFYNNGLPSNLSGGRNPSLDYGFKGA--EIAMASYCS-ELQFLAN 465
>At3g47060.1 68416.m05110 FtsH protease, putative contains
similarity to FtsH protease GI:13183728 from [Medicago
sativa]
Length = 802
Score = 28.7 bits (61), Expect = 2.5
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +1
Query: 151 GVEPSNPKSYSGIPEAEFVDNVDEFMKSPINAEGVDVVLKSLDE 282
G EP ++ +P +EF+ V+ + +GV V+ K D+
Sbjct: 165 GSEPRIQTTFVSVPYSEFLSKVNSNQVQKVEVDGVQVLFKLRDD 208
>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF01480: PWI domain, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 899
Score = 27.9 bits (59), Expect = 4.4
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = +1
Query: 325 KRRRLRQQIPDLARTIEVIEKLKEQKEEVETQFLLSDQV 441
+RR+LR++ DLA ++ E++ E K E Q L Q+
Sbjct: 539 RRRQLREKEDDLADRLKEEEEVAEAKRSAEEQNLQQQQL 577
>At1g10950.1 68414.m01257 endomembrane protein 70, putative
Length = 589
Score = 27.9 bits (59), Expect = 4.4
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = -3
Query: 210 IYKFSFWYSRIRLRVRGFYSVSFHF 136
+Y +S +Y ++ ++ GF+ SF+F
Sbjct: 529 VYLYSIYYYYVKTKMSGFFQTSFYF 553
>At3g19720.2 68416.m02498 dynamin family protein identical to cDNA
dynamin-like protein (ARC5) GI: 30349145; contains Pfam
profile PF00350: Dynamin family
Length = 741
Score = 27.5 bits (58), Expect = 5.8
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 307 EYTLATKRRRLRQQIPDLARTIEVIEKL 390
++++AT RR + Q P+L+RTI V KL
Sbjct: 206 DWSIATTRRIVMQVDPELSRTIVVSTKL 233
>At3g19720.1 68416.m02497 dynamin family protein identical to cDNA
dynamin-like protein (ARC5) GI: 30349145; contains Pfam
profile PF00350: Dynamin family
Length = 777
Score = 27.5 bits (58), Expect = 5.8
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 307 EYTLATKRRRLRQQIPDLARTIEVIEKL 390
++++AT RR + Q P+L+RTI V KL
Sbjct: 206 DWSIATTRRIVMQVDPELSRTIVVSTKL 233
>At1g30190.1 68414.m03690 expressed protein ; expression supported
by MPSS
Length = 278
Score = 27.5 bits (58), Expect = 5.8
Identities = 17/57 (29%), Positives = 31/57 (54%)
Frame = +1
Query: 268 KSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEKLKEQKEEVETQFLLSDQ 438
+S +K R+ ++ T TKR+ ++ D +R + + EK + KEE E LS++
Sbjct: 195 RSKSDKPRRKRLSVDTETTKRKSYGRKKSDCSRWMVIPEKWEYVKEESEEFSKLSNE 251
>At4g11420.1 68417.m01840 eukaryotic translation initiation factor 3
subunit 10 / eIF-3 theta / eIF3a (TIF3A1) identical to
eukaryotic translation initiation factor 3 subunit 10
(eIF-3 theta) (Eukaryotic translation initiation factor
3 large subunit) (eIF3a) (p114). [Arabidopsis thaliana]
SWISS-PROT:Q9LD55
Length = 987
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 238 INAEGVDVVLKSLDEKHRKYKVMEYTLATKRRRLRQQIPDLARTIEVIEK-LKEQKEEVE 414
I ++G+ L E K + M Y + +K +L IP+LA T+E K L +K +E
Sbjct: 513 IESDGLRDHLAVFAESLSKVRAMLYPVPSKASKLAGVIPNLADTVEKEHKRLLARKSIIE 572
>At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 366
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -1
Query: 341 RNRLLLVANVYSITLYLRCFSSRLFKTTSTPSALIGDFINSSTLSTNSASGIPEYDLGFE 162
RNR YS + + C SS S+P ++ G+ N + S N S P F+
Sbjct: 30 RNRTFFSNQNYSRRVLISCSSSLSSDNGSSPDSMNGNG-NGNGSSLNGQSSFPRLP-SFD 87
Query: 161 GSTPSP 144
G++ P
Sbjct: 88 GTSKPP 93
>At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 339
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -1
Query: 341 RNRLLLVANVYSITLYLRCFSSRLFKTTSTPSALIGDFINSSTLSTNSASGIPEYDLGFE 162
RNR YS + + C SS S+P ++ G+ N + S N S P F+
Sbjct: 30 RNRTFFSNQNYSRRVLISCSSSLSSDNGSSPDSMNGNG-NGNGSSLNGQSSFPRLP-SFD 87
Query: 161 GSTPSP 144
G++ P
Sbjct: 88 GTSKPP 93
>At3g13950.1 68416.m01761 expressed protein
Length = 207
Score = 27.1 bits (57), Expect = 7.7
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = -1
Query: 407 SSFCSFNFSITSIVRAKSGICCRNRLLLVANVYSITLYLRCFSSRLF 267
SS SF+ S++ I+ SGI RNR+++ + + + C S+ F
Sbjct: 102 SSTVSFSTSMSLILLVISGIRLRNRMIMAILGTFMVVAVLCISAAFF 148
>At1g47900.1 68414.m05334 expressed protein
Length = 1054
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +1
Query: 163 SNPKSYSGIPEAEFVDNVDEFMKSP 237
S PK+Y+ + E + V+ V FM+SP
Sbjct: 980 SVPKNYAVVDEGDSVNEVPRFMESP 1004
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,568,902
Number of Sequences: 28952
Number of extensions: 170883
Number of successful extensions: 653
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 637
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 651
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -