BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS303F03f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g01800.1 68417.m00237 preprotein translocase secA subunit, pu... 29 1.4 At5g51340.1 68418.m06366 expressed protein 29 1.9 At3g55420.1 68416.m06155 expressed protein 29 1.9 At4g28700.1 68417.m04101 ammonium transporter, putative similar ... 29 2.5 At5g10340.1 68418.m01199 F-box protein-related / SLF-related con... 28 3.3 At3g07620.1 68416.m00912 exostosin family protein contains Pfam ... 27 5.8 >At4g01800.1 68417.m00237 preprotein translocase secA subunit, putative similar to preprotein translocase secA subunit, chloroplast [precursor] SP:Q9SYI0 from [Arabidopsis thaliana]; non-consensus GA donor splice site at exon 4 Length = 1022 Score = 29.5 bits (63), Expect = 1.4 Identities = 13/50 (26%), Positives = 24/50 (48%) Frame = +3 Query: 183 YLQKRKELAFLVDRVLRLTSIFQATGNVSKSWEHHLEIEKLVNEITNIEG 332 YLQKR+ + +++ F N+ + W+ HL+ K V + + G Sbjct: 893 YLQKREIVEKQSPGLMKDAERFLILSNIDRLWKEHLQALKFVQQAVGLRG 942 >At5g51340.1 68418.m06366 expressed protein Length = 726 Score = 29.1 bits (62), Expect = 1.9 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 4/64 (6%) Frame = -2 Query: 358 RVRVFLNWVPSMLVISFTNFSISK*CSQLLLTLPVA*NILVSLSTLST---RKANSFLF- 191 R + L +PS + F N+S+ C LL + P N+LV L++ + +++L+ Sbjct: 72 RSLLLLKSIPSSYDLKFQNYSLLSHCYHLLASFPPQRNLLVKALELASSVPQDISAYLWS 131 Query: 190 CRYN 179 C +N Sbjct: 132 CNFN 135 >At3g55420.1 68416.m06155 expressed protein Length = 216 Score = 29.1 bits (62), Expect = 1.9 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = -2 Query: 481 VKSFEALRPRSYPLNSAISIPSNWTPFSLSHCKYL 377 VKSF R R++ L +S+PS+ +P LS YL Sbjct: 41 VKSFTIRRARTFELLKLLSLPSSSSPPLLSTAAYL 75 >At4g28700.1 68417.m04101 ammonium transporter, putative similar to SP|O04161 Ammonium transporter 1, member 2 (LeAMT1;2) {Lycopersicon esculentum}; contains Pfam profile PF00909: Ammonium Transporter Family Length = 504 Score = 28.7 bits (61), Expect = 2.5 Identities = 10/18 (55%), Positives = 14/18 (77%) Frame = -1 Query: 515 FGWYG*NQRSFSEIFRSF 462 FGWYG N SF+ IF+++ Sbjct: 261 FGWYGFNPGSFATIFKAY 278 >At5g10340.1 68418.m01199 F-box protein-related / SLF-related contains weak hit to Pfam Pfam PF00646: F-box domain and to TIGRFAM TIGR01640 : F-box protein interaction domain; weakly similar to self-incompatibility (S-) locus F-box (GI:29420811) [Prunus mume] Length = 445 Score = 28.3 bits (60), Expect = 3.3 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = -2 Query: 511 DGMVKIRDPSVKSFEALRPRSYPLNSA-ISIPSNW 410 D + +DPS+ SFEALR ++A + IP+ W Sbjct: 131 DPCARQQDPSISSFEALRTFLVESSAASVQIPTPW 165 >At3g07620.1 68416.m00912 exostosin family protein contains Pfam profile: PF03016 Exostosin family Length = 470 Score = 27.5 bits (58), Expect = 5.8 Identities = 18/73 (24%), Positives = 36/73 (49%) Frame = -3 Query: 471 SKL*DQDHXXXXXLFQSHRIGHRFLLATANIYQCEFAEEFEYFLTGFLLCW*FHSLISRF 292 S L D+D+ ++++ HR L +++ EE + + + LC +S+ F Sbjct: 112 SPLGDEDYVPHGDIYRNPYAFHRSYLLMEKMFKIYVYEEGDPPIFHYGLCKDIYSMEGLF 171 Query: 291 LNDVPNFCLRYQS 253 LN + N L+Y++ Sbjct: 172 LNFMENDVLKYRT 184 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,769,218 Number of Sequences: 28952 Number of extensions: 180398 Number of successful extensions: 419 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 413 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 419 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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