BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS303D09f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g54290.1 68414.m06189 eukaryotic translation initiation facto... 110 5e-25
At4g27130.1 68417.m03899 eukaryotic translation initiation facto... 108 2e-24
At5g54760.1 68418.m06820 eukaryotic translation initiation facto... 108 3e-24
At5g54940.2 68418.m06843 eukaryotic translation initiation facto... 104 4e-23
At5g54940.1 68418.m06842 eukaryotic translation initiation facto... 104 4e-23
At5g11900.1 68418.m01392 eukaryotic translation initiation facto... 34 0.067
At1g05530.1 68414.m00567 UDP-glucoronosyl/UDP-glucosyl transfera... 34 0.067
At5g49130.1 68418.m06081 MATE efflux family protein contains Pfa... 30 0.82
At2g15345.1 68415.m01755 expressed protein 30 1.1
At1g12380.1 68414.m01431 expressed protein 29 1.9
At5g66790.1 68418.m08420 protein kinase family protein contains ... 29 2.5
At1g63430.1 68414.m07173 leucine-rich repeat transmembrane prote... 28 4.4
At5g04780.1 68418.m00494 SEC14 cytosolic factor-related contains... 27 5.8
At4g18640.1 68417.m02759 leucine-rich repeat transmembrane prote... 27 5.8
At1g75990.1 68414.m08824 26S proteasome regulatory subunit S3, p... 27 5.8
At1g27900.1 68414.m03419 RNA helicase, putative similar to SP|Q1... 27 5.8
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 27 7.7
At4g02425.1 68417.m00328 expressed protein 27 7.7
At1g56000.1 68414.m06425 amine oxidase-related contains Pfam pro... 27 7.7
>At1g54290.1 68414.m06189 eukaryotic translation initiation factor
SUI1, putative similar to P|P32911 Protein translation
factor SUI1 {Saccharomyces cerevisiae}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 113
Score = 110 bits (265), Expect = 5e-25
Identities = 51/96 (53%), Positives = 65/96 (67%)
Frame = +2
Query: 113 MSIQNLNTFDPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRAC 292
+ +Q FDPFADA VH+R+QQRNGRK+LTTVQGL EY KI++
Sbjct: 4 LEVQVPTAFDPFADANAEDSGAGTKEYVHIRVQQRNGRKSLTTVQGLKKEYSYSKILKDL 63
Query: 293 KKEFACNGTVVEHPEYGEVLQLQGDQRENICQWLTK 400
KKEF CNGTVV+ E G+V+QLQGDQR+N+ +L +
Sbjct: 64 KKEFCCNGTVVQDSELGQVIQLQGDQRKNVSTFLVQ 99
>At4g27130.1 68417.m03899 eukaryotic translation initiation factor
SUI1, putative similar to SP|P32911 Protein translation
factor SUI1 {Saccharomyces cerevisiae}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 113
Score = 108 bits (260), Expect = 2e-24
Identities = 50/88 (56%), Positives = 62/88 (70%)
Frame = +2
Query: 137 FDPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRACKKEFACNG 316
FDPFADA VH+R+QQRNGRK+LTTVQGL EY KI++ KKEF CNG
Sbjct: 12 FDPFADANAEDSGAGTKEYVHIRVQQRNGRKSLTTVQGLKKEYSYTKILKDLKKEFCCNG 71
Query: 317 TVVEHPEYGEVLQLQGDQRENICQWLTK 400
TVV+ E G+V+QLQGDQR+N+ +L +
Sbjct: 72 TVVQDSELGQVIQLQGDQRKNVSTFLVQ 99
>At5g54760.1 68418.m06820 eukaryotic translation initiation factor
SUI1, putative similar to SP|P32911 Protein translation
factor SUI1 {Saccharomyces cerevisiae}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 113
Score = 108 bits (259), Expect = 3e-24
Identities = 50/88 (56%), Positives = 62/88 (70%)
Frame = +2
Query: 137 FDPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRACKKEFACNG 316
FDPFADA VH+R+QQRNGRK+LTTVQGL EY KI++ KKEF CNG
Sbjct: 12 FDPFADANVEDSGAGTKEYVHIRVQQRNGRKSLTTVQGLKKEYSYTKILKDLKKEFCCNG 71
Query: 317 TVVEHPEYGEVLQLQGDQRENICQWLTK 400
TVV+ E G+V+QLQGDQR+N+ +L +
Sbjct: 72 TVVQDSELGQVIQLQGDQRKNVSTFLVQ 99
>At5g54940.2 68418.m06843 eukaryotic translation initiation factor
SUI1, putative similar to SP|P32911 Protein translation
factor SUI1 {Saccharomyces cerevisiae}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 112
Score = 104 bits (249), Expect = 4e-23
Identities = 46/96 (47%), Positives = 69/96 (71%)
Frame = +2
Query: 113 MSIQNLNTFDPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRAC 292
+ IQ + +DPFA+A S ++ +H+RIQQRNG+K+LTTVQGL EY ++I++
Sbjct: 4 LDIQIPSAYDPFAEAKDSDAPGAKEN-IHIRIQQRNGKKSLTTVQGLKKEYSYERILKDL 62
Query: 293 KKEFACNGTVVEHPEYGEVLQLQGDQRENICQWLTK 400
KK+F CNG VV+ E G+++QLQGDQR+ + Q+L +
Sbjct: 63 KKDFCCNGNVVQDKELGKIIQLQGDQRKKVSQFLVQ 98
>At5g54940.1 68418.m06842 eukaryotic translation initiation factor
SUI1, putative similar to SP|P32911 Protein translation
factor SUI1 {Saccharomyces cerevisiae}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 112
Score = 104 bits (249), Expect = 4e-23
Identities = 46/96 (47%), Positives = 69/96 (71%)
Frame = +2
Query: 113 MSIQNLNTFDPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRAC 292
+ IQ + +DPFA+A S ++ +H+RIQQRNG+K+LTTVQGL EY ++I++
Sbjct: 4 LDIQIPSAYDPFAEAKDSDAPGAKEN-IHIRIQQRNGKKSLTTVQGLKKEYSYERILKDL 62
Query: 293 KKEFACNGTVVEHPEYGEVLQLQGDQRENICQWLTK 400
KK+F CNG VV+ E G+++QLQGDQR+ + Q+L +
Sbjct: 63 KKDFCCNGNVVQDKELGKIIQLQGDQRKKVSQFLVQ 98
>At5g11900.1 68418.m01392 eukaryotic translation initiation factor
SUI1 family protein similar to SP|O43583
Density-regulated protein (DRP1 protein) (Smooth muscle
cell associated protein-3) {Homo sapiens}; contains Pfam
profile PF01253: Translation initiation factor SUI1
Length = 198
Score = 33.9 bits (74), Expect = 0.067
Identities = 21/62 (33%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = +2
Query: 215 RNGRKTLTTVQGLSS-EYDLKKIVRACKKEFACNGTVVEHPEYGEVLQLQGDQRENICQW 391
RN RK +T V+GL L + K+FA +VV+ P E + +QGD +I ++
Sbjct: 114 RNKRKCITIVKGLELFGIKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIIYDIVEF 173
Query: 392 LT 397
+T
Sbjct: 174 IT 175
>At1g05530.1 68414.m00567 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 455
Score = 33.9 bits (74), Expect = 0.067
Identities = 23/57 (40%), Positives = 34/57 (59%), Gaps = 3/57 (5%)
Frame = +2
Query: 77 VSLKQRDPTFNRMSIQNLN--TF-DPFADAIKSSEDDVQDGLVHVRIQQRNGRKTLT 238
+S+ R N +++NL+ TF D F D + S+ DDVQ+ LVH +RNG K L+
Sbjct: 41 LSVIHRSMIPNHNNVENLSFLTFSDGFDDGVISNTDDVQNRLVHF---ERNGDKALS 94
>At5g49130.1 68418.m06081 MATE efflux family protein contains Pfam
profile PF01554: MatE Uncharacterized membrane protein
family
Length = 502
Score = 30.3 bits (65), Expect = 0.82
Identities = 19/39 (48%), Positives = 23/39 (58%), Gaps = 4/39 (10%)
Frame = -3
Query: 330 CSTTVP-LHANSFLHARTIFFRSYSEERP---CTVVSVL 226
CS ++P L ANSFLH I+ R P CT+VSVL
Sbjct: 150 CSFSLPDLLANSFLHPLRIYLRCKGTTWPLMWCTLVSVL 188
>At2g15345.1 68415.m01755 expressed protein
Length = 121
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
Frame = +2
Query: 188 GLVHVRIQQ--RNGRKTLTTVQGL--SSEYD-LKKIVRACKKEFACNGTVVE 328
GLV++ QQ R G K L ++GL + Y LKK R+C KE+ + +E
Sbjct: 3 GLVNMVYQQTERLGYKNLEMIKGLDRTENYSKLKKYYRSCVKEYELSNKAIE 54
>At1g12380.1 68414.m01431 expressed protein
Length = 793
Score = 29.1 bits (62), Expect = 1.9
Identities = 19/55 (34%), Positives = 28/55 (50%)
Frame = -2
Query: 484 MLSVDMLCFSRAA*KPCTLSCSGFTKPEFGEPLANILSLVALKLQHLAVLRVLHD 320
ML + + C+ + C+ S SG K F EPL N+L V + A+ V+HD
Sbjct: 452 MLRLPLHCYYDDERRSCSSSSSGSNKVCFYEPLFNLLEDVLSSAR--AIQLVVHD 504
>At5g66790.1 68418.m08420 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 622
Score = 28.7 bits (61), Expect = 2.5
Identities = 21/77 (27%), Positives = 40/77 (51%), Gaps = 5/77 (6%)
Frame = -2
Query: 421 SGFTKPEFGEPLANILSLVALKLQHLAVLRVLH--DRTVAREL---LLACPHDLLQVIFR 257
SGF +F P + + +L +L + + RV+ D + +E+ + A H+L ++ FR
Sbjct: 513 SGFKVIDFTRPYSEV-NLASLAVDRIGRGRVVDIIDPCLNKEINPKMFASIHNLAELAFR 571
Query: 256 GKALHRSQRLTPVSLLD 206
+ HR+ R T V + +
Sbjct: 572 CLSFHRNMRPTMVEITE 588
>At1g63430.1 68414.m07173 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profiles: PF00069
Eukaryotic protein kinase domain, PF00560 Leucine Rich
Repeat; contains 1 predicted transmembrane domain
Length = 664
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +2
Query: 266 DLKKIVRACKKEFACNGTVVEHPEYGEVLQLQGDQRENI 382
++ R E+A NGT+ EH YGE + +R I
Sbjct: 429 EISPFTRMLVFEYASNGTLYEHLHYGEAALVSWARRMKI 467
>At5g04780.1 68418.m00494 SEC14 cytosolic factor-related contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; contains Pfam profile PF01535:
PPR repeat (three copies)
Length = 864
Score = 27.5 bits (58), Expect = 5.8
Identities = 17/58 (29%), Positives = 33/58 (56%), Gaps = 2/58 (3%)
Frame = -2
Query: 283 HDLLQVIFRGKALHRSQRLT-PVSLLDTDVD*TILNVVLRAFDSIGEWVECV-EILDG 116
H++LQ+ R A+ ++ + +D + D T+LNV++ A+ G +VE ++ DG
Sbjct: 57 HEILQLCARNGAVMEAKACHGKIIRIDLEGDVTLLNVLINAYSKCG-FVELARQVFDG 113
>At4g18640.1 68417.m02759 leucine-rich repeat transmembrane protein
kinase, putative
Length = 686
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +2
Query: 299 EFACNGTVVEHPEYGEVLQLQGDQRENICQWLTKFRL 409
E+A NGT+ EH V +L + E++ W + R+
Sbjct: 465 EYAPNGTLFEHLHVNNVFRLSDKETEHL-DWSARMRI 500
>At1g75990.1 68414.m08824 26S proteasome regulatory subunit S3,
putative (RPN3) similar to 26S proteasome regulatory
subunit S3 SP:P93768 [Nicotiana tabacum (Common
tobacco)]
Length = 487
Score = 27.5 bits (58), Expect = 5.8
Identities = 19/75 (25%), Positives = 38/75 (50%), Gaps = 6/75 (8%)
Frame = +2
Query: 17 RAHNSALSVTVILLSA*YRSVSLKQRDPTFNRMSIQN------LNTFDPFADAIKSSEDD 178
R HN + + ++ R++S+ +++R+S+Q+ LN+ +P ADA
Sbjct: 342 RTHNLIVRLRHNVIRTGLRNISI-----SYSRISLQDVAQKLRLNSANPVADAESIVAKA 396
Query: 179 VQDGLVHVRIQQRNG 223
++DG + I +NG
Sbjct: 397 IRDGAIDATIDHKNG 411
>At1g27900.1 68414.m03419 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 700
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/60 (26%), Positives = 29/60 (48%)
Frame = +1
Query: 40 SDSYIVECVVSQRFVETKRPYVQSYVHPESQHIRPIRRCYQKLGGRRSRWFSPRPYPATK 219
+D + VV + T RP++++ + + PI+R +KL R+ S P P+ K
Sbjct: 604 NDGMMPNYVVYHELISTTRPFMRNVCAVDMAWVAPIKRKIEKLNVRK---LSGGPAPSFK 660
>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 513
Score = 27.1 bits (57), Expect = 7.7
Identities = 18/66 (27%), Positives = 31/66 (46%)
Frame = -3
Query: 216 RCWIRTWTKPS*TSSSELLIASANGSNVLRFWMDIRLNVGSLCFNETLRYYALNNITVTD 37
R W R W +P + +S+++I + SN L + LN+ R + L + T+ D
Sbjct: 256 RMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIA--------REFGLQSATIGD 307
Query: 36 NALLCA 19
+CA
Sbjct: 308 EVDICA 313
>At4g02425.1 68417.m00328 expressed protein
Length = 262
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/42 (45%), Positives = 24/42 (57%), Gaps = 4/42 (9%)
Frame = +3
Query: 294 RRSSRATVRSWSTRS-TARCCSFRA---TSERIFASGSPNSG 407
RR+SR+ S RS T RCCS A S+ FA G+ +SG
Sbjct: 101 RRNSRSVSGRSSDRSGTRRCCSIGAHGTCSDLPFAVGTDSSG 142
>At1g56000.1 68414.m06425 amine oxidase-related contains Pfam
profile PF01593: amine oxidase, flavin-containing
Length = 384
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/53 (35%), Positives = 24/53 (45%)
Frame = +2
Query: 176 DVQDGLVHVRIQQRNGRKTLTTVQGLSSEYDLKKIVRACKKEFACNGTVVEHP 334
D + ++H N T +Q LSSE L KI KEF C+G V P
Sbjct: 269 DSERWILHSTPDYANSVIAKTGLQKLSSE-TLNKISEEMFKEFQCSGLVSSLP 320
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,233,840
Number of Sequences: 28952
Number of extensions: 226847
Number of successful extensions: 722
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 722
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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