SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS303D05f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot...    34   0.067
At3g27710.1 68416.m03460 zinc finger protein-related contains si...    33   0.088
At3g27720.1 68416.m03461 zinc finger protein-related contains Pf...    33   0.15 
At3g30550.1 68416.m03866 hypothetical protein includes At2g05890...    32   0.20 
At4g34370.1 68417.m04883 IBR domain-containing protein similar t...    30   0.82 
At4g39510.1 68417.m05587 cytochrome P450 family protein contains...    30   1.1  
At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3...    30   1.1  
At5g63450.1 68418.m07965 cytochrome P450, putative                     28   3.3  
At4g40020.1 68417.m05666 hypothetical protein                          28   3.3  
At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3...    28   4.4  
At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3...    28   4.4  
At4g13040.1 68417.m02035 AP2 domain-containing transcription fac...    28   4.4  
At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|...    27   5.8  
At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|...    27   5.8  
At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly...    27   5.8  
At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd...    27   5.8  
At3g13540.1 68416.m01702 myb family transcription factor contain...    27   5.8  
At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3...    27   5.8  
At4g31110.1 68417.m04415 wall-associated kinase, putative simila...    27   7.7  
At4g31100.1 68417.m04414 wall-associated kinase, putative              27   7.7  
At3g18590.1 68416.m02363 plastocyanin-like domain-containing pro...    27   7.7  
At1g53310.1 68414.m06042 phosphoenolpyruvate carboxylase, putati...    27   7.7  
At1g10610.1 68414.m01202 basic helix-loop-helix (bHLH) family pr...    27   7.7  
At1g03380.1 68414.m00317 expressed protein                             27   7.7  

>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein,
            Xenopus laevis, PIR:T30335
          Length = 1229

 Score = 33.9 bits (74), Expect = 0.067
 Identities = 32/117 (27%), Positives = 54/117 (46%), Gaps = 6/117 (5%)
 Frame = +2

Query: 8    KMKT-VQVILCLFVASLYANETSVSDSKLEDDLYNSI--LVADYDHSVEKSKQIYEDKKS 178
            ++KT VQ I C+      A+ET++  SK  DDL   I  L+ D +  +E  +Q+ E+  S
Sbjct: 714  EVKTMVQAIACVSQREAEAHETAIKLSKENDDLRQKIKVLIEDNNKLIELYEQVAEENSS 773

Query: 179  EVITNVVNKLIRNN--KMNCMEYAYQLWLQGSKDIVRECFPVEFRLI-FAENNIKLM 340
                 +      NN    N  E A ++    +++  +    +E +L    + N KLM
Sbjct: 774  RAWGKIETDSSSNNADAQNSAEIALEVEKSAAEEQKKMIGNLENQLTEMHDENEKLM 830


>At3g27710.1 68416.m03460 zinc finger protein-related contains
           similarity to zinc finger proteins and Pfam domain,
           PF01485: IBR domain
          Length = 537

 Score = 33.5 bits (73), Expect = 0.088
 Identities = 18/72 (25%), Positives = 36/72 (50%)
 Frame = +2

Query: 56  YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 235
           Y  +  +   KLED L  SIL     +S  K ++++  K+   +T+ VN+L  + ++   
Sbjct: 353 YRYKAHIDSLKLEDKLRKSILEKAVSNSETKDQKVF--KEYSWVTDAVNRLFISRRILSQ 410

Query: 236 EYAYQLWLQGSK 271
            Y +  ++ G +
Sbjct: 411 SYPFAFYMFGEE 422


>At3g27720.1 68416.m03461 zinc finger protein-related contains
           Pfam:PF01485 IBR domain
          Length = 493

 Score = 32.7 bits (71), Expect = 0.15
 Identities = 23/86 (26%), Positives = 43/86 (50%)
 Frame = +2

Query: 56  YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 235
           Y  +  +   KLED L  SIL     +S  K ++++  K+   I + VN+L R+ ++   
Sbjct: 313 YRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVF--KEYSWIIDAVNRLFRSRRILSY 370

Query: 236 EYAYQLWLQGSKDIVRECFPVEFRLI 313
            Y +  ++ G K++ ++    E R I
Sbjct: 371 SYPFVFYMFG-KELFKDDMSDEERNI 395


>At3g30550.1 68416.m03866 hypothetical protein includes At2g05890,
           At4g07450, At3g30630, At3g43100, At2g09960, At3g30550,
           At1g39430, At2g10460, At4g03640, At5g35250
          Length = 509

 Score = 32.3 bits (70), Expect = 0.20
 Identities = 19/66 (28%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
 Frame = +2

Query: 8   KMKTVQVILCLFVASLYANETSVSDSKL-EDDLYNSILVADYDHSVEKSKQIYEDKKSEV 184
           K KT Q +LC+   +++    S + +++  D++ + I+  DYD  V+ S  I  ++ S +
Sbjct: 398 KGKTFQFLLCVQRENIFGGYDSFTVARVYTDNIADEIVQEDYDAYVDPSSLISIEQDSLM 457

Query: 185 ITNVVN 202
           +TN V+
Sbjct: 458 LTNGVD 463


>At4g34370.1 68417.m04883 IBR domain-containing protein similar to
           SP|Q94981 Ariadne-1 protein (Ari-1) {Drosophila
           melanogaster}; contains Pfam profile PF01485: IBR domain
          Length = 597

 Score = 30.3 bits (65), Expect = 0.82
 Identities = 29/113 (25%), Positives = 55/113 (48%), Gaps = 6/113 (5%)
 Frame = +2

Query: 83  SKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWLQ 262
           SKLED L ++I    ++   +  K+  + K    +TN +++L R+ ++    YA+  ++ 
Sbjct: 364 SKLEDKLRDTI----HEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMF 419

Query: 263 GSKDIVRECFPVEFRL---IFAENNIKLMYKRDGLALTLD---DENSNDGRLA 403
           G +    E  P E  +   +F +   +L    + L+  L+   DE SND  +A
Sbjct: 420 GEEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMA 472


>At4g39510.1 68417.m05587 cytochrome P450 family protein contains
           Pfam PF00067: Cytochrome P450; similar to  Cytochrome
           P450 86A2 (SP:O23066) [Arabidopsis thaliana]
          Length = 508

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 22/57 (38%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
 Frame = +2

Query: 323 NNIKLMYKRDGLALTLDDENSNDGRLAYG-DGKDKTSPKVSWKFVPLWENNKVYFKI 490
           ++IKL   +  L    DD+   D  LA+   G+D TS  +SW F  L EN +V  KI
Sbjct: 281 SHIKLDTTKYELLNPSDDKFLRDTILAFNLAGRDTTSSALSWFFWLLSENPQVVTKI 337


>At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3.1)
           (GLR2) identical to putative glutamate receptor GLR2
           [Arabidopsis thaliana] gi|4185740|gb|AAD09174; plant
           glutamate receptor family, PMID:11379626
          Length = 951

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 15/35 (42%), Positives = 22/35 (62%)
 Frame = +2

Query: 350 DGLALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 454
           D +AL  DD+NS +G  A GD  ++   K+S+K V
Sbjct: 194 DVVALYNDDDNSRNGVTALGDELEERRCKISYKAV 228


>At5g63450.1 68418.m07965 cytochrome P450, putative
          Length = 510

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 13/33 (39%), Positives = 21/33 (63%)
 Frame = +2

Query: 413 GKDKTSPKVSWKFVPLWENNKVYFKIVNTQRNQ 511
           G+D TS  ++W F  L +N+ V  KI++  RN+
Sbjct: 306 GRDTTSAAMTWLFWLLSQNDDVETKILDELRNK 338


>At4g40020.1 68417.m05666 hypothetical protein
          Length = 615

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 21/96 (21%), Positives = 49/96 (51%)
 Frame = +2

Query: 104 YNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYAYQLWLQGSKDIVR 283
           Y+S  VAD    + K K   E++++EV++     L ++  +  +E A +  L   KD  R
Sbjct: 17  YSSNKVADIGTELYKMKASLENRENEVVSLKQELLKKDIFIKNLEAAEKKLLDSFKDQSR 76

Query: 284 ECFPVEFRLIFAENNIKLMYKRDGLALTLDDENSND 391
           E    E + +  E+ +++   ++ +  + + ++S++
Sbjct: 77  EL--EETKALVEESKVEIASLKEKIDTSYNSQDSSE 110


>At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3.2)
           (GLUR2) identical to putative glutamate receptor
           like-protein GLUR2 [Arabidopsis thaliana]
           gi|13160471|gb|AAK13248; plant glutamate receptor
           family, PMID:11379626
          Length = 912

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = +2

Query: 356 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 454
           +AL  DD+NS +G  A GD  +    K+S+K V
Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198


>At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3.2)
           (GLUR2) identical to putative glutamate receptor
           like-protein GLUR2 [Arabidopsis thaliana]
           gi|13160471|gb|AAK13248; plant glutamate receptor
           family, PMID:11379626
          Length = 912

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = +2

Query: 356 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 454
           +AL  DD+NS +G  A GD  +    K+S+K V
Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198


>At4g13040.1 68417.m02035 AP2 domain-containing transcription factor
           family protein contains Pfam profile PF00847: AP2 domain
          Length = 226

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 11/45 (24%), Positives = 23/45 (51%)
 Frame = +3

Query: 78  PTPNSKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSY 212
           P PN++  +    +  +TT+ +++     R+RR +S  M   + Y
Sbjct: 36  PNPNARAAYNPGPAETVTTVIVEKKAIEERSRRTRSKHMHFRSDY 80


>At5g57180.2 68418.m07143 expressed protein ; supporting cDNA
           gi|13991645|gb|AF359387.1|AF359387
          Length = 435

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
 Frame = +3

Query: 66  KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 191
           K +SP P+S T  T A+S P +TI      PL  + R  RT+R + +Q
Sbjct: 21  KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67


>At5g57180.1 68418.m07142 expressed protein ; supporting cDNA
           gi|13991645|gb|AF359387.1|AF359387
          Length = 424

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
 Frame = +3

Query: 66  KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 191
           K +SP P+S T  T A+S P +TI      PL  + R  RT+R + +Q
Sbjct: 21  KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67


>At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative /
           polyphosphoinositide-binding protein, putative similar
           to polyphosphoinositide binding protein SEC14 homolog
           Ssh1p  (GI:2739044) {Glycine max}
          Length = 341

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +2

Query: 320 ENNIKLMYKRDGLALTLDDENSNDGRLAYGD 412
           E+ +     R+GLA+++DD  S+   LA GD
Sbjct: 310 ESELHKFENRNGLAVSIDDRKSSHETLANGD 340


>At4g00620.1 68417.m00086 tetrahydrofolate
           dehydrogenase/cyclohydrolase, putative similar to
           SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
           (C1-THF synthase) [Includes: Methylenetetrahydrofolate
           dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
           cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
           synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
           contains Pfam profiles PF02882: Tetrahydrofolate
           dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
           PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
           catalytic domain
          Length = 360

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 3/29 (10%)
 Frame = -2

Query: 415 AIAVGKSAIV---AVLIVQRQSETVALVH 338
           A+ +G+S IV   A L++QR+  TV+++H
Sbjct: 230 AVVIGRSNIVGMPAALLLQREDATVSIIH 258


>At3g13540.1 68416.m01702 myb family transcription factor contains
           Pfam profile: PF00249 myb-like DNA-binding domain
          Length = 249

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 15/53 (28%), Positives = 29/53 (54%)
 Frame = +2

Query: 44  VASLYANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVN 202
           ++S + ++T+V+    + D  NSI V   +H  E     Y+DK S  + +++N
Sbjct: 163 ISSSHTDDTTVNGG--DGDSKNSINVFGGEHGYEDFGFCYDDKFSSFLNSLIN 213


>At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3.5)
           plant glutamate receptor family, PMID:11379626
          Length = 895

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
 Frame = +2

Query: 347 RDGLALTLDDENSNDGRLAYGDGKDKTSPKVSWK--FVPLWENNKV 478
           R+ +A+ +DDE   +G    GD   K   K+S+K  F P  +N+ +
Sbjct: 123 REVVAIFVDDEYGRNGISVLGDALAKKRAKISYKAAFPPGADNSSI 168


>At4g31110.1 68417.m04415 wall-associated kinase, putative similar
           to wall-associated kinase 1, Arabidopsis thaliana,
           gb:AJ009696
          Length = 756

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +2

Query: 101 LYNSILVADYDHSVEKSKQIYEDKKSEVITNVV 199
           +Y  +LV     +V+KSK I EDK  E I  VV
Sbjct: 430 VYKGMLVDGRTVAVKKSKVIDEDKLQEFINEVV 462


>At4g31100.1 68417.m04414 wall-associated kinase, putative
          Length = 786

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +2

Query: 101 LYNSILVADYDHSVEKSKQIYEDKKSEVITNVV 199
           +Y  +LV     +V+KSK I EDK  E I  VV
Sbjct: 458 VYKGMLVDGRTVAVKKSKVIDEDKLQEFINEVV 490


>At3g18590.1 68416.m02363 plastocyanin-like domain-containing
           protein
          Length = 188

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/28 (39%), Positives = 20/28 (71%)
 Frame = +3

Query: 72  QSPTPNSKTIFTTASSLPITTIPLKRAN 155
           +SP P+S +  +++SSLP +T   K++N
Sbjct: 134 ESPPPSSSSSSSSSSSLPASTPKAKKSN 161


>At1g53310.1 68414.m06042 phosphoenolpyruvate carboxylase, putative
           / PEP carboxylase, putative (PPC1) strong similarity to
           SP|P29196 Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
           (PEPCASE) {Solanum tuberosum}; contains Pfam profile
           PF00311: phosphoenolpyruvate carboxylase
          Length = 967

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/30 (36%), Positives = 21/30 (70%)
 Frame = -2

Query: 451 ELPADFWTRLVLAIAVGKSAIVAVLIVQRQ 362
           ELPAD +   ++++A   S ++AV ++QR+
Sbjct: 519 ELPADSFGAYIISMATAPSDVLAVELLQRE 548


>At1g10610.1 68414.m01202 basic helix-loop-helix (bHLH) family
           protein contains Pfam profile: PF00010 helix-loop-helix
           DNA-binding domain
          Length = 421

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 15/57 (26%), Positives = 28/57 (49%)
 Frame = +2

Query: 56  YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKM 226
           Y NE  V   KLED+L   I   +      + +    D ++E +++  NK ++ N++
Sbjct: 305 YINELLVEKQKLEDEL-KGINEMECKEIAAEEQSAIADPEAERVSSKSNKRVKKNEV 360


>At1g03380.1 68414.m00317 expressed protein
          Length = 926

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 9/29 (31%), Positives = 20/29 (68%)
 Frame = +2

Query: 434 KVSWKFVPLWENNKVYFKIVNTQRNQYLT 520
           KV+   +P+W+N+K+ F ++++ R+   T
Sbjct: 671 KVTSGMLPVWQNSKISFHVMDSPRDSSST 699


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,903,218
Number of Sequences: 28952
Number of extensions: 158597
Number of successful extensions: 737
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 716
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 737
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -