BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS303C02f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosoli... 172 1e-43 At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative... 84 5e-17 At1g64530.1 68414.m07315 RWP-RK domain-containing protein simila... 31 0.36 At2g05755.1 68415.m00619 integral membrane family protein contai... 30 0.82 At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase... 29 1.9 At1g74370.1 68414.m08616 zinc finger (C3HC4-type RING finger) fa... 29 1.9 At5g11000.1 68418.m01281 expressed protein 28 3.3 At2g32080.2 68415.m03921 PUR alpha-1 protein identical to PUR al... 28 3.3 At2g32080.1 68415.m03920 PUR alpha-1 protein identical to PUR al... 28 3.3 At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein ... 28 4.4 At3g09130.1 68416.m01074 hypothetical protein 28 4.4 At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR... 28 4.4 At5g14330.1 68418.m01675 expressed protein 27 5.8 At3g43500.1 68416.m04612 hypothetical protein 27 5.8 >At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosolic (PGIC) identical to SP|P34795 Glucose-6-phosphate isomerase, cytosolic (EC 5.3.1.9) (GPI) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI) {Arabidopsis thaliana}; contains Pfam profile PF00342: glucose-6-phosphate isomerase Length = 560 Score = 172 bits (418), Expect = 1e-43 Identities = 92/180 (51%), Positives = 116/180 (64%), Gaps = 7/180 (3%) Frame = +2 Query: 2 FSDQVVSGQWKGYTGKAITDVINIGIGGSDLGPLMVTEALKPYANHLK------VHFVSN 163 FSD++ SG W G TGK + DVI IGIGGS LGPL V AL+ L+ + F++N Sbjct: 130 FSDKIRSGSWVGATGKPLKDVIAIGIGGSFLGPLFVHTALQTDPEALESAKGRQLRFLAN 189 Query: 164 IDGTHLAEVLKKLNPETALFIIASKTFTTQETITNASSAKTWFLESAKDPSAVARHFVAL 343 ID +A + LNPET L ++ SKTFTT ET+ NA + + W + +A SAVA+H VA+ Sbjct: 190 IDPVDVARNISGLNPETTLVVVVSKTFTTAETMLNARTLREW-ITAALGASAVAKHMVAV 248 Query: 344 STNAEKVTAFGIDANNMFGFWDWVGGRYSLWSAIG-LSISLYIGHENFEKLLDGANFMDQ 520 STN V FGID NN F FWDWVGGRYS+ SA+G L +SL G EK L GA+ +DQ Sbjct: 249 STNLALVEKFGIDPNNAFAFWDWVGGRYSVCSAVGVLPLSLQYGFSMVEKFLKGASSIDQ 308 >At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative similar to glucose-6-phosphate isomerase [Spinacia oleracea] GI:3413511; contains Pfam profile PF00342: glucose-6-phosphate isomerase Length = 613 Score = 84.2 bits (199), Expect = 5e-17 Identities = 61/181 (33%), Positives = 92/181 (50%), Gaps = 8/181 (4%) Frame = +2 Query: 2 FSDQVVSGQWKGYTGKA--ITDVINIGIGGSDLGPLMVTEALKPYANHLKVHFVSNIDGT 175 FSD ++SG+ K + T ++++GIGGS LGP V EAL P LK+ F+ N D Sbjct: 165 FSDDIISGKIKPPSSPEGRFTQILSVGIGGSALGPQFVAEALAPDNPPLKIRFIDNTDPA 224 Query: 176 HLAEVLKKLNPETA--LFIIASKTFTTQETITNASSAKTWFLES----AKDPSAVARHFV 337 + + +L PE A L ++ SK+ T ET + F E+ AK A+ + Sbjct: 225 GIDHQIAQLGPELASTLVVVISKSGGTPETRNGLLEVQKAFREAGLNFAKQGVAITQE-N 283 Query: 338 ALSTNAEKVTAFGIDANNMFGFWDWVGGRYSLWSAIGLSISLYIGHENFEKLLDGANFMD 517 +L N ++ + F +DWVGGR S+ SA+GL + G N ++L GA MD Sbjct: 284 SLLDNTARIEGW----LARFPMYDWVGGRTSIMSAVGLLPAALQG-INVREMLTGAALMD 338 Query: 518 Q 520 + Sbjct: 339 E 339 >At1g64530.1 68414.m07315 RWP-RK domain-containing protein similar to nodule inception protein [Lotus japonicus] GI:6448579; contains Pfam profile: PF02042 RWP-RK domain Length = 841 Score = 31.5 bits (68), Expect = 0.36 Identities = 13/36 (36%), Positives = 20/36 (55%) Frame = -2 Query: 472 SDVQRDGQTDRGPQRISAPDPVPEAEHVVSVDTERG 365 SD + + D G +R DP+PEA+ V + +RG Sbjct: 516 SDFMENNEVDDGVERFQTLDPIPEAKTVKKSERKRG 551 >At2g05755.1 68415.m00619 integral membrane family protein contains Pfam PF00892: Integral membrane protein domain Length = 401 Score = 30.3 bits (65), Expect = 0.82 Identities = 15/37 (40%), Positives = 18/37 (48%) Frame = +2 Query: 359 KVTAFGIDANNMFGFWDWVGGRYSLWSAIGLSISLYI 469 KVT I F W W G RYS + LS +LY+ Sbjct: 73 KVTETEISPVTQFSSWIWSGSRYSGLLCMALSSTLYL 109 >At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase (MAPKK), putative (MKK3) similar to NPK2 [Nicotiana tabacum] gi|862342|dbj|BAA06731; mitogen-activated protein kinase kinase (MAPKK) family, PMID:12119167 Length = 520 Score = 29.1 bits (62), Expect = 1.9 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Frame = +2 Query: 110 TEALKPYANHLKVHFVSNIDG-THLAEVLKKLNPETALFIIASKTFTTQETITNASS 277 T+ LK A+ L +H+ S DG L K L ET++F + K T I +A S Sbjct: 360 TQRLKDLADMLTIHYYSLFDGFDDLWHHAKSLYTETSVFSFSGKHNTGSTEIFSALS 416 >At1g74370.1 68414.m08616 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) Length = 261 Score = 29.1 bits (62), Expect = 1.9 Identities = 15/43 (34%), Positives = 25/43 (58%) Frame = -3 Query: 288 HVLAELAFVIVSCVVNVLDAMMNSAVSGFSFFKTSARWVPSML 160 H+LA+ V++ ++ + +++AV G FF T A VPS L Sbjct: 201 HLLAKFPLVVIFLLMALYAIPVSAAVLGVYFFVTFALAVPSFL 243 >At5g11000.1 68418.m01281 expressed protein Length = 389 Score = 28.3 bits (60), Expect = 3.3 Identities = 13/30 (43%), Positives = 16/30 (53%) Frame = -3 Query: 450 RPIADHSEYLPPTQSQKPNMLLASIPNAVT 361 RP ADH+ P SQ P+ L + PN T Sbjct: 7 RPTADHNHPPPQPPSQSPSPLSSGDPNLTT 36 >At2g32080.2 68415.m03921 PUR alpha-1 protein identical to PUR alpha-1 GI:5081612 from [Arabidopsis thaliana]; contains Pfam profile: PF04845 PurA ssDNA and RNA-binding protein Length = 295 Score = 28.3 bits (60), Expect = 3.3 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = +2 Query: 197 KLNPETALFIIASKTFTTQETITNASSAKTWFLE 298 K NP I+ KT T+ TI SS +WFL+ Sbjct: 48 KENPRGRYLKISEKTSATRSTIIVPSSGISWFLD 81 >At2g32080.1 68415.m03920 PUR alpha-1 protein identical to PUR alpha-1 GI:5081612 from [Arabidopsis thaliana]; contains Pfam profile: PF04845 PurA ssDNA and RNA-binding protein Length = 296 Score = 28.3 bits (60), Expect = 3.3 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = +2 Query: 197 KLNPETALFIIASKTFTTQETITNASSAKTWFLE 298 K NP I+ KT T+ TI SS +WFL+ Sbjct: 48 KENPRGRYLKISEKTSATRSTIIVPSSGISWFLD 81 >At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) Length = 675 Score = 27.9 bits (59), Expect = 4.4 Identities = 16/58 (27%), Positives = 26/58 (44%) Frame = -3 Query: 462 SEMDRPIADHSEYLPPTQSQKPNMLLASIPNAVTFSAFVDKATKCLATAEGSLADSRN 289 S +P D+S+ QSQ+ + L P ++F +F + K L A S+N Sbjct: 545 SSASKPNTDNSDSQTLKQSQQGSFLPLGPPKGISFLSFASEEQKTLNREPQKPASSKN 602 >At3g09130.1 68416.m01074 hypothetical protein Length = 397 Score = 27.9 bits (59), Expect = 4.4 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +1 Query: 16 SKRAMEGVYRKGYNRCDQHWYW 81 SKRA V R+ ++RCDQ +W Sbjct: 183 SKRAFSAVERQFFSRCDQLSWW 204 >At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR1) identical to microtubule organization 1 protein GI:14317953 from [Arabidopsis thaliana] Length = 1978 Score = 27.9 bits (59), Expect = 4.4 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = +3 Query: 96 VPSWSQKH*SPMLIILRSISYLTSTAPTWPR 188 VP W++K+ ++ I+Y++STA +P+ Sbjct: 650 VPGWNEKNVQVQQQVIEIITYISSTAAKFPK 680 >At5g14330.1 68418.m01675 expressed protein Length = 128 Score = 27.5 bits (58), Expect = 5.8 Identities = 19/71 (26%), Positives = 32/71 (45%), Gaps = 4/71 (5%) Frame = -3 Query: 516 SMKLAPSRSFSKFS--CPMYSEMDRPIADHS--EYLPPTQSQKPNMLLASIPNAVTFSAF 349 ++++AP S +F+ C + + M H+ + P + P M A P+A TFSA+ Sbjct: 53 NIQMAPRSSLIRFAFICIVLAVMVMTAESHNGTHHHGPAMAPMPPMAKAPSPSAATFSAY 112 Query: 348 VDKATKCLATA 316 L A Sbjct: 113 PQLIATALVAA 123 >At3g43500.1 68416.m04612 hypothetical protein Length = 153 Score = 27.5 bits (58), Expect = 5.8 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -2 Query: 472 SDVQRDGQTDRGPQRISAPDPVPEAEH 392 +D+ DG TDR P R+ A +P E H Sbjct: 8 ADIAADGLTDRPPDRL-ADEPAEELSH 33 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,990,812 Number of Sequences: 28952 Number of extensions: 207775 Number of successful extensions: 824 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 804 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 821 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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