BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS303C02f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosoli... 172 1e-43
At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative... 84 5e-17
At1g64530.1 68414.m07315 RWP-RK domain-containing protein simila... 31 0.36
At2g05755.1 68415.m00619 integral membrane family protein contai... 30 0.82
At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase... 29 1.9
At1g74370.1 68414.m08616 zinc finger (C3HC4-type RING finger) fa... 29 1.9
At5g11000.1 68418.m01281 expressed protein 28 3.3
At2g32080.2 68415.m03921 PUR alpha-1 protein identical to PUR al... 28 3.3
At2g32080.1 68415.m03920 PUR alpha-1 protein identical to PUR al... 28 3.3
At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein ... 28 4.4
At3g09130.1 68416.m01074 hypothetical protein 28 4.4
At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR... 28 4.4
At5g14330.1 68418.m01675 expressed protein 27 5.8
At3g43500.1 68416.m04612 hypothetical protein 27 5.8
>At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosolic
(PGIC) identical to SP|P34795 Glucose-6-phosphate
isomerase, cytosolic (EC 5.3.1.9) (GPI) (Phosphoglucose
isomerase) (PGI) (Phosphohexose isomerase) (PHI)
{Arabidopsis thaliana}; contains Pfam profile PF00342:
glucose-6-phosphate isomerase
Length = 560
Score = 172 bits (418), Expect = 1e-43
Identities = 92/180 (51%), Positives = 116/180 (64%), Gaps = 7/180 (3%)
Frame = +2
Query: 2 FSDQVVSGQWKGYTGKAITDVINIGIGGSDLGPLMVTEALKPYANHLK------VHFVSN 163
FSD++ SG W G TGK + DVI IGIGGS LGPL V AL+ L+ + F++N
Sbjct: 130 FSDKIRSGSWVGATGKPLKDVIAIGIGGSFLGPLFVHTALQTDPEALESAKGRQLRFLAN 189
Query: 164 IDGTHLAEVLKKLNPETALFIIASKTFTTQETITNASSAKTWFLESAKDPSAVARHFVAL 343
ID +A + LNPET L ++ SKTFTT ET+ NA + + W + +A SAVA+H VA+
Sbjct: 190 IDPVDVARNISGLNPETTLVVVVSKTFTTAETMLNARTLREW-ITAALGASAVAKHMVAV 248
Query: 344 STNAEKVTAFGIDANNMFGFWDWVGGRYSLWSAIG-LSISLYIGHENFEKLLDGANFMDQ 520
STN V FGID NN F FWDWVGGRYS+ SA+G L +SL G EK L GA+ +DQ
Sbjct: 249 STNLALVEKFGIDPNNAFAFWDWVGGRYSVCSAVGVLPLSLQYGFSMVEKFLKGASSIDQ 308
>At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative
similar to glucose-6-phosphate isomerase [Spinacia
oleracea] GI:3413511; contains Pfam profile PF00342:
glucose-6-phosphate isomerase
Length = 613
Score = 84.2 bits (199), Expect = 5e-17
Identities = 61/181 (33%), Positives = 92/181 (50%), Gaps = 8/181 (4%)
Frame = +2
Query: 2 FSDQVVSGQWKGYTGKA--ITDVINIGIGGSDLGPLMVTEALKPYANHLKVHFVSNIDGT 175
FSD ++SG+ K + T ++++GIGGS LGP V EAL P LK+ F+ N D
Sbjct: 165 FSDDIISGKIKPPSSPEGRFTQILSVGIGGSALGPQFVAEALAPDNPPLKIRFIDNTDPA 224
Query: 176 HLAEVLKKLNPETA--LFIIASKTFTTQETITNASSAKTWFLES----AKDPSAVARHFV 337
+ + +L PE A L ++ SK+ T ET + F E+ AK A+ +
Sbjct: 225 GIDHQIAQLGPELASTLVVVISKSGGTPETRNGLLEVQKAFREAGLNFAKQGVAITQE-N 283
Query: 338 ALSTNAEKVTAFGIDANNMFGFWDWVGGRYSLWSAIGLSISLYIGHENFEKLLDGANFMD 517
+L N ++ + F +DWVGGR S+ SA+GL + G N ++L GA MD
Sbjct: 284 SLLDNTARIEGW----LARFPMYDWVGGRTSIMSAVGLLPAALQG-INVREMLTGAALMD 338
Query: 518 Q 520
+
Sbjct: 339 E 339
>At1g64530.1 68414.m07315 RWP-RK domain-containing protein similar
to nodule inception protein [Lotus japonicus]
GI:6448579; contains Pfam profile: PF02042 RWP-RK domain
Length = 841
Score = 31.5 bits (68), Expect = 0.36
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = -2
Query: 472 SDVQRDGQTDRGPQRISAPDPVPEAEHVVSVDTERG 365
SD + + D G +R DP+PEA+ V + +RG
Sbjct: 516 SDFMENNEVDDGVERFQTLDPIPEAKTVKKSERKRG 551
>At2g05755.1 68415.m00619 integral membrane family protein contains
Pfam PF00892: Integral membrane protein domain
Length = 401
Score = 30.3 bits (65), Expect = 0.82
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +2
Query: 359 KVTAFGIDANNMFGFWDWVGGRYSLWSAIGLSISLYI 469
KVT I F W W G RYS + LS +LY+
Sbjct: 73 KVTETEISPVTQFSSWIWSGSRYSGLLCMALSSTLYL 109
>At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase
(MAPKK), putative (MKK3) similar to NPK2 [Nicotiana
tabacum] gi|862342|dbj|BAA06731; mitogen-activated
protein kinase kinase (MAPKK) family, PMID:12119167
Length = 520
Score = 29.1 bits (62), Expect = 1.9
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = +2
Query: 110 TEALKPYANHLKVHFVSNIDG-THLAEVLKKLNPETALFIIASKTFTTQETITNASS 277
T+ LK A+ L +H+ S DG L K L ET++F + K T I +A S
Sbjct: 360 TQRLKDLADMLTIHYYSLFDGFDDLWHHAKSLYTETSVFSFSGKHNTGSTEIFSALS 416
>At1g74370.1 68414.m08616 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 261
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/43 (34%), Positives = 25/43 (58%)
Frame = -3
Query: 288 HVLAELAFVIVSCVVNVLDAMMNSAVSGFSFFKTSARWVPSML 160
H+LA+ V++ ++ + +++AV G FF T A VPS L
Sbjct: 201 HLLAKFPLVVIFLLMALYAIPVSAAVLGVYFFVTFALAVPSFL 243
>At5g11000.1 68418.m01281 expressed protein
Length = 389
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -3
Query: 450 RPIADHSEYLPPTQSQKPNMLLASIPNAVT 361
RP ADH+ P SQ P+ L + PN T
Sbjct: 7 RPTADHNHPPPQPPSQSPSPLSSGDPNLTT 36
>At2g32080.2 68415.m03921 PUR alpha-1 protein identical to PUR
alpha-1 GI:5081612 from [Arabidopsis thaliana]; contains
Pfam profile: PF04845 PurA ssDNA and RNA-binding protein
Length = 295
Score = 28.3 bits (60), Expect = 3.3
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 197 KLNPETALFIIASKTFTTQETITNASSAKTWFLE 298
K NP I+ KT T+ TI SS +WFL+
Sbjct: 48 KENPRGRYLKISEKTSATRSTIIVPSSGISWFLD 81
>At2g32080.1 68415.m03920 PUR alpha-1 protein identical to PUR
alpha-1 GI:5081612 from [Arabidopsis thaliana]; contains
Pfam profile: PF04845 PurA ssDNA and RNA-binding protein
Length = 296
Score = 28.3 bits (60), Expect = 3.3
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 197 KLNPETALFIIASKTFTTQETITNASSAKTWFLE 298
K NP I+ KT T+ TI SS +WFL+
Sbjct: 48 KENPRGRYLKISEKTSATRSTIIVPSSGISWFLD 81
>At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 675
Score = 27.9 bits (59), Expect = 4.4
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = -3
Query: 462 SEMDRPIADHSEYLPPTQSQKPNMLLASIPNAVTFSAFVDKATKCLATAEGSLADSRN 289
S +P D+S+ QSQ+ + L P ++F +F + K L A S+N
Sbjct: 545 SSASKPNTDNSDSQTLKQSQQGSFLPLGPPKGISFLSFASEEQKTLNREPQKPASSKN 602
>At3g09130.1 68416.m01074 hypothetical protein
Length = 397
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 16 SKRAMEGVYRKGYNRCDQHWYW 81
SKRA V R+ ++RCDQ +W
Sbjct: 183 SKRAFSAVERQFFSRCDQLSWW 204
>At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR1)
identical to microtubule organization 1 protein
GI:14317953 from [Arabidopsis thaliana]
Length = 1978
Score = 27.9 bits (59), Expect = 4.4
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = +3
Query: 96 VPSWSQKH*SPMLIILRSISYLTSTAPTWPR 188
VP W++K+ ++ I+Y++STA +P+
Sbjct: 650 VPGWNEKNVQVQQQVIEIITYISSTAAKFPK 680
>At5g14330.1 68418.m01675 expressed protein
Length = 128
Score = 27.5 bits (58), Expect = 5.8
Identities = 19/71 (26%), Positives = 32/71 (45%), Gaps = 4/71 (5%)
Frame = -3
Query: 516 SMKLAPSRSFSKFS--CPMYSEMDRPIADHS--EYLPPTQSQKPNMLLASIPNAVTFSAF 349
++++AP S +F+ C + + M H+ + P + P M A P+A TFSA+
Sbjct: 53 NIQMAPRSSLIRFAFICIVLAVMVMTAESHNGTHHHGPAMAPMPPMAKAPSPSAATFSAY 112
Query: 348 VDKATKCLATA 316
L A
Sbjct: 113 PQLIATALVAA 123
>At3g43500.1 68416.m04612 hypothetical protein
Length = 153
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -2
Query: 472 SDVQRDGQTDRGPQRISAPDPVPEAEH 392
+D+ DG TDR P R+ A +P E H
Sbjct: 8 ADIAADGLTDRPPDRL-ADEPAEELSH 33
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,990,812
Number of Sequences: 28952
Number of extensions: 207775
Number of successful extensions: 824
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 804
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 821
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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