Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302H07f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 223 6e-59
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 223 7e-59
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 222 1e-58
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr... 33 0.15
At1g68030.1 68414.m07772 PHD finger protein-related contains low... 33 0.15
At2g46980.2 68415.m05869 expressed protein 29 1.4
At2g46980.1 68415.m05868 expressed protein 29 1.4
At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains... 28 3.3
At4g40050.1 68417.m05671 expressed protein 28 3.3
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 4.4
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 4.4
At4g08470.1 68417.m01398 mitogen-activated protein kinase, putat... 28 4.4
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138... 28 4.4
At5g06940.1 68418.m00784 leucine-rich repeat family protein cont... 27 5.8
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 223 bits (545), Expect = 6e-59
Identities = 98/142 (69%), Positives = 119/142 (83%)
Frame = +2
Query: 95 PVVDILGDEMTRIIWDLIKEKLIIPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 274
P+V++ GDEMTR+IW +IKEKLI+P+LD+++ +DLG+ NRD TDD+VT++ AEA KYN
Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140
Query: 275 VGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPI 454
V IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI
Sbjct: 141 VAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPI 200
Query: 455 IIGRHAHADQYKATDFVVPGAG 520
IGRHA DQY+ATD V+ G G
Sbjct: 201 CIGRHAFGDQYRATDTVIKGPG 222
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 223 bits (544), Expect = 7e-59
Identities = 101/148 (68%), Positives = 122/148 (82%), Gaps = 1/148 (0%)
Frame = +2
Query: 80 KIK-AGPVVDILGDEMTRIIWDLIKEKLIIPFLDIELHVYDLGMENRDKTDDQVTIDCAE 256
KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE
Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64
Query: 257 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 436
A KKYNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV
Sbjct: 65 ATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVP 124
Query: 437 GWDKPIIIGRHAHADQYKATDFVVPGAG 520
GW KPI IGRHA DQY+ATD V+ G G
Sbjct: 125 GWTKPICIGRHAFGDQYRATDAVIKGPG 152
>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
limon]; Nicotiana tabacum SP|P50218
Length = 416
Score = 222 bits (543), Expect = 1e-58
Identities = 103/148 (69%), Positives = 119/148 (80%), Gaps = 1/148 (0%)
Frame = +2
Query: 80 KIKA-GPVVDILGDEMTRIIWDLIKEKLIIPFLDIELHVYDLGMENRDKTDDQVTIDCAE 256
KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE
Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64
Query: 257 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 436
A KYNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV
Sbjct: 65 ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVP 124
Query: 437 GWDKPIIIGRHAHADQYKATDFVVPGAG 520
GW KPI IGRHA DQY+ATD +V G
Sbjct: 125 GWTKPICIGRHAFGDQYRATDLIVNEPG 152
>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
protein similar to SP|P09171 tRNA pseudouridine synthase
B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
(Pseudouridylate synthase) (Uracil hydrolyase)
{Escherichia coli O157:H7}; contains Pfam profile
PF01509: TruB family pseudouridylate synthase (N
terminal domain)
Length = 540
Score = 32.7 bits (71), Expect = 0.15
Identities = 27/101 (26%), Positives = 43/101 (42%)
Frame = +2
Query: 149 KEKLIIPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEF 328
K+K++ V +GMEN D+ DD++ K+ + + EK+VE+F
Sbjct: 222 KKKVVAALGGKRSEVKRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQF 281
Query: 329 KLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKP 451
K + KSPN + + G E + L WD P
Sbjct: 282 -FKGLTKSPNE--KGMASGGGDGEPFLVTRNGELPPRWDGP 319
>At1g68030.1 68414.m07772 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 314
Score = 32.7 bits (71), Expect = 0.15
Identities = 17/36 (47%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Frame = -2
Query: 145 KIPDYSGHFITKDVNNR--SSLYFRHIVSVTGVTTT 44
K+PDY G + + V R +SL+ H+VSVT TTT
Sbjct: 49 KLPDYLGEYTNEMVAFRCLASLFDSHVVSVTTTTTT 84
>At2g46980.2 68415.m05869 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 481 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 368
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At2g46980.1 68415.m05868 expressed protein
Length = 516
Score = 29.5 bits (63), Expect = 1.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 481 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 368
+S SNDD + P + S I ++ PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266
>At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains
Pfam PF01277: Oleosin domain
Length = 150
Score = 28.3 bits (60), Expect = 3.3
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = -3
Query: 168 GIISFSLIRSQIILVISSPRMSTTGPAFILDILYL 64
G++SFS + + + +I+SP + PAF++ + L
Sbjct: 41 GLMSFSFVATVTLFLIASPLLLIFAPAFMVTVAVL 75
>At4g40050.1 68417.m05671 expressed protein
Length = 599
Score = 28.3 bits (60), Expect = 3.3
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
Frame = -2
Query: 487 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 338
VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596
>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
Length = 160
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +1
Query: 400 GYYLQEYTETCNRVGQTHHHWTSCSC 477
G+YL E++ TC +V HH C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147
>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
family protein similar to katanin p80 subunit
[Strongylocentrotus purpuratus] GI:3005601; contains
Pfam profile PF00400: WD domain, G-beta repeat
Length = 871
Score = 27.9 bits (59), Expect = 4.4
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
Frame = +2
Query: 113 GDEMTRIIWDLIKEKLIIPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 265
G++ +WDL KL+ F LD H + L + DKT ++ E I
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314
Query: 266 K---YNVGIKCATITPDEKRV 319
G++C T PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335
>At4g08470.1 68417.m01398 mitogen-activated protein kinase, putative
similar to mitogen-activated protein kinase [Arabidopsis
thaliana] gi|1255448|dbj|BAA09057; contains Pfam
PF00069: Protein kinase domain
Length = 560
Score = 27.9 bits (59), Expect = 4.4
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 4/84 (4%)
Frame = +2
Query: 254 EAIKKYNVGIK--CATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNI-- 421
EAI +VG + C+ +TP + + +K S G + + +N
Sbjct: 237 EAISDSSVGSESTCSLMTPSMEFPDRISFRKRDFSEEGPSGRVKEKRKLMRNKLIENFRK 296
Query: 422 PRLVTGWDKPIIIGRHAHADQYKA 493
P +T W K ++GR ++A Y+A
Sbjct: 297 PEDITSWLKGQLLGRGSYASVYEA 320
>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'auxin-independent growth promoter -related' based on
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 512
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3
Query: 396 LKTVPPRILRMVPLGLFH 343
LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193
>At5g06940.1 68418.m00784 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 872
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 496 SCFVLISMSMTSND-DGFVPPCYKSRYILANNSLPENSTTKNITDG 362
+C L+S+S+ N G +PP ++L L +NS T I G
Sbjct: 434 NCKKLVSLSLAGNAFTGEIPPSLADLHVLTYLDLSDNSLTGLIPQG 479
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,795,194
Number of Sequences: 28952
Number of extensions: 248188
Number of successful extensions: 636
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 636
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -