Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302H05f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27880.1 68415.m03380 argonaute protein, putative / AGO, puta... 71 4e-13
At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille prot... 66 1e-11
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 64 5e-11
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 59 2e-09
At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi do... 45 3e-05
At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi do... 30 0.82
At3g27925.1 68416.m03484 DegP protease, putative SP:022609; almo... 29 2.5
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 27 5.8
At5g56310.1 68418.m07028 pentatricopeptide (PPR) repeat-containi... 27 7.7
At3g14740.2 68416.m01864 PHD finger family protein similar to zi... 27 7.7
At3g14740.1 68416.m01863 PHD finger family protein similar to zi... 27 7.7
>At2g27880.1 68415.m03380 argonaute protein, putative / AGO,
putative similar to SP|O04379 Argonaute protein (AGO1)
{Arabidopsis thaliana}; contains Pfam profiles PF02170:
PAZ domain, PF02171: Piwi domain
Length = 997
Score = 71.3 bits (167), Expect = 4e-13
Identities = 51/167 (30%), Positives = 80/167 (47%), Gaps = 11/167 (6%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVVIK---DMNGKDMPFEVSFKASGIVDYNNVLKHM 174
A+D K+ Y+ PLP ++ + V + K +GKD PF+V+ K D + + +
Sbjct: 215 AYDGRKSLYTAGPLP-FDSKEFVVNLAEKRADGSSGKDRPFKVAVKNVTSTDLYQLQQFL 273
Query: 175 ATGGSSLNAPTDTIQCIDIVLKQGTLESYVKAGRQYFMRPASPI------DLGDGLEMWT 336
AP DTIQ +D+VL+ YV GR +F +LGDG+E W
Sbjct: 274 DR--KQREAPYDTIQVLDVVLRDKPSNDYVSVGRSFFHTSLGKDARDGRGELGDGIEYWR 331
Query: 337 GLFQSAIFTSKAF-INVDVAHKGFPKNQPMIDAFTRDFRL-DPNRPM 471
G FQS T +N+DV+ + F + + D ++ + D NRP+
Sbjct: 332 GYFQSLRLTQMGLSLNIDVSARSFYEPIVVTDFISKFLNIRDLNRPL 378
>At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille protein
(ZWILLE) identical to SP|Q9XGW1 PINHEAD protein (ZWILLE
protein) {Arabidopsis thaliana}; contains Pfam profiles
PF02171: Piwi domain, PF02170: PAZ domain
Length = 988
Score = 66.5 bits (155), Expect = 1e-11
Identities = 43/149 (28%), Positives = 82/149 (55%), Gaps = 7/149 (4%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVVIKD---MNG--KDMPFEVSFKASGIVDYNNVLK 168
A+D K+ Y+ LP T + + VK+V +D +NG ++ ++V+ K + +++ +
Sbjct: 196 AYDGRKSLYTAGELP-FTWKEFSVKIVDEDDGIINGPKRERSYKVAIKFVARANMHHLGE 254
Query: 169 HMATGGSSLNAPTDTIQCIDIVLKQGTLESYVKAGRQYFMRPA-SPIDLGDGLEMWTGLF 345
+A G + P + +Q +DIVL++ +++ + GR +F +P LG+GLE W G +
Sbjct: 255 FLA--GKRADCPQEAVQILDIVLRELSVKRFCPVGRSFFSPDIKTPQRLGEGLESWCGFY 312
Query: 346 QSAIFTSKAF-INVDVAHKGFPKNQPMID 429
QS T +N+D+A F + P+I+
Sbjct: 313 QSIRPTQMGLSLNIDMASAAFIEPLPVIE 341
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 64.1 bits (149), Expect = 5e-11
Identities = 45/168 (26%), Positives = 79/168 (47%), Gaps = 10/168 (5%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVVIKDMNG----KDMPFEVSFKASGIVDYNNVLKH 171
A+D K+ Y+ PLP ++ + + ++ +++ ++ F+V K D +++
Sbjct: 247 AYDGRKSLYTAGPLP-FNSKEFRINLLDEEVGAGGQRREREFKVVIKLVARADLHHL--G 303
Query: 172 MATGGSSLNAPTDTIQCIDIVLKQGTLESYVKAGRQYFMRP-ASPIDLGDGLEMWTGLFQ 348
M G +AP + +Q +DIVL++ Y+ GR ++ LGDGLE W G +Q
Sbjct: 304 MFLEGKQSDAPQEALQVLDIVLRELPTSRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQ 363
Query: 349 SAIFTSKAF-INVDVAHKGFPKNQPMI----DAFTRDFRLDPNRPMDR 477
S T +N+D++ F + P+I D RD P DR
Sbjct: 364 SIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISSRPLSDADR 411
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 58.8 bits (136), Expect = 2e-09
Identities = 45/170 (26%), Positives = 79/170 (46%), Gaps = 12/170 (7%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVVIKDMNG----KDMPFEVSFKASGIVDYNNVLKH 171
A+D K+ Y+ PLP ++ + + ++ +++ ++ F+V K D +++
Sbjct: 247 AYDGRKSLYTAGPLP-FNSKEFRINLLDEEVGAGGQRREREFKVVIKLVARADLHHL--G 303
Query: 172 MATGGSSLNAPTDTIQCIDIVLKQGTLES--YVKAGRQYFMRP-ASPIDLGDGLEMWTGL 342
M G +AP + +Q +DIVL++ Y+ GR ++ LGDGLE W G
Sbjct: 304 MFLEGKQSDAPQEALQVLDIVLRELPTSRIRYIPVGRSFYSPDIGKKQSLGDGLESWRGF 363
Query: 343 FQSAIFTSKAF-INVDVAHKGFPKNQPMI----DAFTRDFRLDPNRPMDR 477
+QS T +N+D++ F + P+I D RD P DR
Sbjct: 364 YQSIRPTQMGLSLNIDMSSTAFIEANPVIQFVCDLLNRDISSRPLSDADR 413
>At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 1013
Score = 45.2 bits (102), Expect = 3e-05
Identities = 30/101 (29%), Positives = 52/101 (51%), Gaps = 1/101 (0%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVV-IKDMNGKDMPFEVSFKASGIVDYNNVLKHMAT 180
A+D KN +S LP T Y V+ ++M G+ F + K ++ ++ ++M T
Sbjct: 234 AYDGQKNIFSAVELP---TGSYKVEYPKTEEMRGRSYTFTI--KQVNVLKLGDLKEYM-T 287
Query: 181 GGSSLNAPTDTIQCIDIVLKQGTLESYVKAGRQYFMRPASP 303
G SS N P D +Q +D+V+K+ + + G+ +F R P
Sbjct: 288 GRSSFN-PRDVLQGMDVVMKEHPSKCMITVGKSFFTRETEP 327
>At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi
domain-containing protein contains Pfam profiles
PF02170: PAZ domain, PF02171: Piwi domain
Length = 1194
Score = 30.3 bits (65), Expect = 0.82
Identities = 26/108 (24%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +1
Query: 4 AFDQTKNCYSLTPLPKITTERYGVKVVIKDMNGKDMPFEVS-FKASGIVDYNNVLKHMAT 180
A+D KN +S LP + + + M G+ F + K ++D +
Sbjct: 407 AYDGQKNIFSAVELPT-GSFKVDFSETEEIMRGRSYTFIIKQVKELKLLDLQAYID---- 461
Query: 181 GGSSLNAPTDTIQCIDIVLKQGTLESYVKAGRQYFMRPASPIDLGDGL 324
G S P D +Q +D+V+K+ + + G+++F ID G G+
Sbjct: 462 -GRSTFIPRDVLQGMDVVMKEHPSKRMITVGKRFFSTRLE-IDFGYGV 507
>At3g27925.1 68416.m03484 DegP protease, putative SP:022609; almost
identical to DegP protease precursor GB:AF028842 from
[Arabidopsis thaliana] (J. Biol. Chem. 273 (12),
7094-7098 (1998))
Length = 439
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 314 ATGSRCGLVYSNLLYLHRKPSSTLMLHTKVSQR 412
AT + C L+ S+ L+LH PSS L S R
Sbjct: 2 ATTTSCSLLLSSTLFLHSPPSSHLSFFNLSSSR 34
>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
putative similar to SP|Q03460 Glutamate synthase [NADH],
chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
{Medicago sativa}
Length = 2208
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = -1
Query: 365 DVNIADWNKPVHISSPSPKSIGDAGRMKYCLPAFTYDSNVPC 240
+V + DWN+ + S P P + R C F + N C
Sbjct: 1719 NVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGC 1760
>At5g56310.1 68418.m07028 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 530
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = -1
Query: 311 KSIGDAGRMKYCLPAFTYDSNVPCFNTMSIHCMVSVGALRDDP 183
++ +AG ++Y FT+ PC NT + M+ +L D+P
Sbjct: 55 EACSNAGHLRYAYSVFTHQ---PCPNTYLHNTMIRALSLLDEP 94
>At3g14740.2 68416.m01864 PHD finger family protein similar to
zinc-finger protein BR140 [PIR|JC2069][Homo sapiens];
contains PHD-finger domain PF00628
Length = 343
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +3
Query: 255 IICESRQTILHASCISD*LGRRARDVDWFIPIC 353
+ C+ ++HASC + L + + DWF C
Sbjct: 168 VFCDGCDLMVHASCYGNPLVKAIPEGDWFCRQC 200
>At3g14740.1 68416.m01863 PHD finger family protein similar to
zinc-finger protein BR140 [PIR|JC2069][Homo sapiens];
contains PHD-finger domain PF00628
Length = 341
Score = 27.1 bits (57), Expect = 7.7
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = +3
Query: 255 IICESRQTILHASCISD*LGRRARDVDWFIPIC 353
+ C+ ++HASC + L + + DWF C
Sbjct: 168 VFCDGCDLMVHASCYGNPLVKAIPEGDWFCRQC 200
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,586,605
Number of Sequences: 28952
Number of extensions: 277265
Number of successful extensions: 668
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 665
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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