BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS302H01f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 30 0.18 SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces ... 28 0.73 SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 27 1.3 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 1.3 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 27 1.7 SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 27 2.2 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.2 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 26 3.9 SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|S... 26 3.9 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 5.2 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 5.2 SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces ... 25 5.2 SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schi... 25 5.2 SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 6.8 SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomy... 25 6.8 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 9.0 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 25 9.0 SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi... 25 9.0 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 9.0 >SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 648 Score = 30.3 bits (65), Expect = 0.18 Identities = 15/57 (26%), Positives = 30/57 (52%), Gaps = 4/57 (7%) Frame = +2 Query: 218 LEKNESAYIQNLSVLYSNRRRHRAC----LLKPLPNVTIPNISTNLSLEEYNSICGK 376 L ++ Y+Q L V + H+ C L+KP PN+ + +I+ L++++ + K Sbjct: 185 LRNVDAKYVQALVVAPTRELAHQICQHFELIKPSPNIRVMSITGGLAVQKQQRLLNK 241 >SPCC1259.13 |chk1|rad27|Chk1 protein kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 496 Score = 28.3 bits (60), Expect = 0.73 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = +2 Query: 239 YIQNLSVLYSNRRRHRACLLKPLPNVTIPNISTNLSL 349 Y++N ++LY N R CLL+ + + + N+ NL L Sbjct: 426 YVRNQTILYVNLHDKRKCLLQGV--IELTNLGHNLEL 460 >SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1398 Score = 27.5 bits (58), Expect = 1.3 Identities = 22/93 (23%), Positives = 39/93 (41%), Gaps = 2/93 (2%) Frame = +2 Query: 191 DELEGWPVFLEKNESAYIQNLSVLYS--NRRRHRACLLKPLPNVTIPNISTNLSLEEYNS 364 D+L F E + +L V +S N+R+ C++ + +P L L E Sbjct: 1028 DDLASLKHFYEDPQIVACSSLGVYHSIFNKRKFDYCIIDEASQIPLPICLGPLQLAEKFV 1087 Query: 365 ICGKVLDICEARKSDCASEDGSTVVNAKLKEQK 463 + G + K+ S+DG ++ KL +K Sbjct: 1088 LVGDHYQLPPLVKNSRTSKDGLSLSLFKLLSEK 1120 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 27.5 bits (58), Expect = 1.3 Identities = 15/56 (26%), Positives = 26/56 (46%) Frame = -1 Query: 320 WLHLVKVLAGTHDVVYGLSIEPIDFECKRSHSFLKILAILLTRRQCLRENYIRLLQ 153 W+H K + + L+ E EC+R+ F + LA + +C R ++ LQ Sbjct: 555 WIHAAKSSYINYAKQFPLADETYKLECQRNTYFARWLAACRSDPRCRRLDFQHFLQ 610 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 27.1 bits (57), Expect = 1.7 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +2 Query: 50 EKMQEIIKYSKRLYFVPVAVISTKENDVV 136 E +E++KY L +PVA S+K+ D++ Sbjct: 598 EMYEEVLKYCLELKEIPVASNSSKKTDLI 626 >SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 26.6 bits (56), Expect = 2.2 Identities = 16/61 (26%), Positives = 30/61 (49%), Gaps = 1/61 (1%) Frame = +2 Query: 299 KPLPNVTIPNISTNLS-LEEYNSICGKVLDICEARKSDCASEDGSTVVNAKLKEQKTIYT 475 K L N+ ++S +L LEEYN+ C +L + + + ++ S + L+ + I Sbjct: 97 KSLSNINFFSLSNDLKRLEEYNTSCSVLLSLYQRQLKKISTTIESYYLADTLRHNQNILN 156 Query: 476 L 478 L Sbjct: 157 L 157 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.2 Identities = 20/74 (27%), Positives = 35/74 (47%), Gaps = 1/74 (1%) Frame = +2 Query: 143 NLYFEA-SGCSSLVNIVDELEGWPVFLEKNESAYIQNLSVLYSNRRRHRACLLKPLPNVT 319 N +FE+ + CS+ +++ V+ Y + V S R+ + KP+ + Sbjct: 979 NQWFESVTICSNFFVGDNKVNAIGVYDSGRRLLYGTDTGVYVSLRKANSPLQFKPVRALN 1038 Query: 320 IPNISTNLSLEEYN 361 IPNIS +EEY+ Sbjct: 1039 IPNISQLEVIEEYS 1052 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.8 bits (54), Expect = 3.9 Identities = 10/31 (32%), Positives = 20/31 (64%) Frame = +2 Query: 305 LPNVTIPNISTNLSLEEYNSICGKVLDICEA 397 +P + I ++S +LSLE+YN ++ + +A Sbjct: 561 IPRLWILSVSPSLSLEQYNKCMREIRSLSDA 591 >SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 25.8 bits (54), Expect = 3.9 Identities = 12/42 (28%), Positives = 20/42 (47%) Frame = +2 Query: 11 ENQICTNWNKGAIEKMQEIIKYSKRLYFVPVAVISTKENDVV 136 EN + +KG + K E I Y + ++ ++ K ND V Sbjct: 616 ENSEASTISKGVVLKYLETISYKVSIIYLEKLLLDNKFNDTV 657 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 25.4 bits (53), Expect = 5.2 Identities = 15/37 (40%), Positives = 22/37 (59%) Frame = +2 Query: 299 KPLPNVTIPNISTNLSLEEYNSICGKVLDICEARKSD 409 +PL VT N ++N SLEE N+ V D+ E+ K + Sbjct: 1165 EPLDVVTDDNTNSNFSLEETNA---SVTDMPESEKHE 1198 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 25.4 bits (53), Expect = 5.2 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 266 SNRRRHRACLLKPLPNVTIPNISTNLSLEEYN 361 S+R+ + L+P+ + +PNIS +EE+N Sbjct: 851 SSRKSNNGSCLEPIFKLQLPNISQLDVIEEHN 882 >SPBC1703.02 |rsc9||RSC complex subunit Rsc9|Schizosaccharomyces pombe|chr 2|||Manual Length = 780 Score = 25.4 bits (53), Expect = 5.2 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = -1 Query: 269 LSIEPIDFECKRSHSFLKILAILLTRRQCLRENYIRLLQNTNFPT 135 + I P DF SH+F A+ ++ Q E I ++ FPT Sbjct: 568 MGISPADFIKVSSHAFYNARAMTVSTPQLPVEYVINGIKRRKFPT 612 >SPAC17A5.09c |||protein phosphatase regulatory subunit Glc9|Schizosaccharomyces pombe|chr 1|||Manual Length = 310 Score = 25.4 bits (53), Expect = 5.2 Identities = 12/39 (30%), Positives = 18/39 (46%) Frame = +2 Query: 272 RRRHRACLLKPLPNVTIPNISTNLSLEEYNSICGKVLDI 388 R++H + KPL + + T LE NS G D+ Sbjct: 266 RKKHYFAMAKPLKRDELESSGTESDLERDNSDSGSASDV 304 >SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces pombe|chr 2|||Manual Length = 857 Score = 25.0 bits (52), Expect = 6.8 Identities = 15/29 (51%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = +2 Query: 212 VFLEKNESAYIQNLSV-LYSNRRRHRACL 295 +FLE E Y Q V L S RR RACL Sbjct: 11 LFLEAKEEEYKQRRRVPLDSRRRVRRACL 39 >SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 333 Score = 25.0 bits (52), Expect = 6.8 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 35 NKGAIEKMQEIIKYSKRLYFVPVAVISTKENDVV 136 N A+E + E IKY + FV ++TKE ++ Sbjct: 96 NTVALENIYERIKYPDDISFVENQAVTTKEPIII 129 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 24.6 bits (51), Expect = 9.0 Identities = 8/17 (47%), Positives = 13/17 (76%) Frame = -3 Query: 174 ELHPLASKYKFPNTTSF 124 +LHPL S+++FP + F Sbjct: 471 KLHPLWSRFRFPKDSEF 487 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 24.6 bits (51), Expect = 9.0 Identities = 18/54 (33%), Positives = 25/54 (46%) Frame = +2 Query: 302 PLPNVTIPNISTNLSLEEYNSICGKVLDICEARKSDCASEDGSTVVNAKLKEQK 463 P+PN+ IS SLEEY + D E RK ++ GS V + +K Sbjct: 249 PVPNIKHAPISLTASLEEYLN----DPDFEENRKQYLQNKSGSPVEETAILNRK 298 >SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schizosaccharomyces pombe|chr 3|||Manual Length = 524 Score = 24.6 bits (51), Expect = 9.0 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = -3 Query: 201 SNSSTMFTRELHPLASKYKFPNTTSFS 121 ++S T +LH SK+KFPN + S Sbjct: 131 TSSLTGLMSQLHFFISKWKFPNFSDLS 157 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 24.6 bits (51), Expect = 9.0 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = +2 Query: 47 IEKMQEIIKYSKRLYFVPVAVISTKENDVVLGNLYFEA 160 + K+ +K S +LYF+P+ V + + D G L F+A Sbjct: 1215 VVKLLRALKISHQLYFMPLCVYNFEFYD---GGLMFQA 1249 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,202,249 Number of Sequences: 5004 Number of extensions: 47426 Number of successful extensions: 185 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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