Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302F03f
(489 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 40 0.001
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 38 0.003
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 34 0.045
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 34 0.059
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 34 0.059
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 31 0.32
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 1.7
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 2.2
At5g04290.1 68418.m00422 KOW domain-containing transcription fac... 29 2.2
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 5.1
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 6.8
At2g01960.1 68415.m00131 expressed protein 27 6.8
At5g44290.1 68418.m05421 protein kinase family protein contains ... 27 9.0
At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 27 9.0
At3g22410.1 68416.m02827 expressed protein 27 9.0
At2g45135.1 68415.m05617 expressed protein ; expression supporte... 27 9.0
At1g02280.1 68414.m00169 GTP-binding protein (TOC33) identical t... 27 9.0
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 39.5 bits (88), Expect = 0.001
Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 8/77 (10%)
Frame = +1
Query: 277 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 432
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 433 SVESRLSSDGVLSXIAP 483
+++ + +GVLS P
Sbjct: 126 EIKASM-ENGVLSVTVP 141
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 38.3 bits (85), Expect = 0.003
Identities = 27/77 (35%), Positives = 40/77 (51%), Gaps = 8/77 (10%)
Frame = +1
Query: 277 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 432
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 433 SVESRLSSDGVLSXIAP 483
V++ + +GVLS P
Sbjct: 125 EVKASM-ENGVLSVTVP 140
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 34.3 bits (75), Expect = 0.045
Identities = 23/77 (29%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +1
Query: 277 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 432
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 433 SVESRLSSDGVLSXIAP 483
V++ + +GVL+ + P
Sbjct: 128 EVKATM-ENGVLTVVVP 143
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 33.9 bits (74), Expect = 0.059
Identities = 25/84 (29%), Positives = 46/84 (54%), Gaps = 8/84 (9%)
Frame = +1
Query: 244 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 399
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 400 RYALPEGCTAESVESRLSSDGVLS 471
++ LP+ ESV+++L +GVL+
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLT 158
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.9 bits (74), Expect = 0.059
Identities = 24/77 (31%), Positives = 40/77 (51%), Gaps = 8/77 (10%)
Frame = +1
Query: 277 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 432
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 433 SVESRLSSDGVLSXIAP 483
V++ + +GVL+ P
Sbjct: 122 QVKAAM-ENGVLTVTVP 137
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 31.5 bits (68), Expect = 0.32
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 8/77 (10%)
Frame = +1
Query: 277 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 432
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 433 SVESRLSSDGVLSXIAP 483
V++ + +GVL+ P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.1 bits (62), Expect = 1.7
Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -3
Query: 442 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 272
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 2.2
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +1
Query: 298 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 459
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At5g04290.1 68418.m00422 KOW domain-containing transcription factor
family protein
Length = 1493
Score = 28.7 bits (61), Expect = 2.2
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = +3
Query: 219 GSRPWLQHQK*QG*VPSKPGRPAFRPGRNLC*NGGRLHRG*RQT 350
GS+PW +H + G FR GRN GGR G R +
Sbjct: 1321 GSKPWNEHSGGGRGFGERRGGGGFRGGRNQSGRGGRSFDGGRSS 1364
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 5.1
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -3
Query: 463 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 320
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 6.8
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +1
Query: 313 KTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDG 462
+ D Y EG EE+ ++ R Y LPE E+VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At2g01960.1 68415.m00131 expressed protein
Length = 260
Score = 27.1 bits (57), Expect = 6.8
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 144 RFSQCCSRPPCEQRILPPVASPCC 215
RF + PP ++R+LP V + CC
Sbjct: 147 RFVNSYALPPYDRRLLPSVKTGCC 170
>At5g44290.1 68418.m05421 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 644
Score = 26.6 bits (56), Expect = 9.0
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 362 KISMVTYRVNSLVATLCRRAVRLNLWSPGCL 454
++ + YR L+ C V ++LWS GC+
Sbjct: 297 RVVTLWYRPPELLLGACHYGVGVDLWSTGCI 327
>At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPA) GI:2944178; contains Pfam profile PF02358:
Trehalose-phosphatase
Length = 377
Score = 26.6 bits (56), Expect = 9.0
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = +1
Query: 97 PRRLMDQHFGLGLTPEDFLSAAAGPLVSREYYRPWRHLAAAARDLGSSIKSDKD 258
PR+ + + F + + PED S A L + H+AA A++ ++ D D
Sbjct: 62 PRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115
>At3g22410.1 68416.m02827 expressed protein
Length = 400
Score = 26.6 bits (56), Expect = 9.0
Identities = 22/95 (23%), Positives = 39/95 (41%)
Frame = +1
Query: 79 DYEIERPRRLMDQHFGLGLTPEDFLSAAAGPLVSREYYRPWRHLAAAARDLGSSIKSDKD 258
D ++E RL+ + L E F + V R ++ AA+ L S +
Sbjct: 5 DEKVEAVLRLVKKQSPLTFKQEKFCNREC---VERFLKVKGDNVKKAAKQLSSCLS---- 57
Query: 259 KFQVNLDVQHFAPEEISVKTADGYIVVEGKHEEKK 363
++ N D++ EE S + +DG + G E +
Sbjct: 58 -WRQNFDIERLGAEEFSTELSDGVAYISGHDRESR 91
>At2g45135.1 68415.m05617 expressed protein ; expression supported
by MPSS
Length = 304
Score = 26.6 bits (56), Expect = 9.0
Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 110 WINIS-AWG*LRKIFSVLQPAPL*AENTTARGVTL 211
WI++S +WG LR SVL+P L + + G TL
Sbjct: 270 WISLSYSWGHLRTASSVLKPPALRSLYFNSLGTTL 304
>At1g02280.1 68414.m00169 GTP-binding protein (TOC33) identical to
atToc33 protein (GI:11557973) [Arabidopsis thaliana];
Carboxyl-terminal end highly similar to GTP-binding
protein SP:U43377, location of EST gb|AA394770 and
gb|R30089; identical to cDNA for chloroplast atToc33
protein GI:11557972
Length = 297
Score = 26.6 bits (56), Expect = 9.0
Identities = 21/83 (25%), Positives = 37/83 (44%)
Frame = -1
Query: 258 ILVTFDAGAKVSSRGSKVTPRAVVFSAHKGAGCSTEKIFRSQPQAEMLIHEATRTLNFVI 79
+L T AG+K+ + + + AVV++ + G +K ++ P E I + + V
Sbjct: 182 LLKTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDV- 240
Query: 78 K*PRNQRHFVVVDL*RCSLSFED 10
NQR + VD S+ D
Sbjct: 241 --ATNQRKAIHVDKKMVDGSYSD 261
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,855,046
Number of Sequences: 28952
Number of extensions: 234410
Number of successful extensions: 626
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 626
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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