BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS302F01f (521 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g25530.1 68415.m03056 AFG1-like ATPase family protein contain... 60 9e-10 At4g30490.1 68417.m04329 AFG1-like ATPase family protein contain... 57 6e-09 At4g28070.1 68417.m04026 AFG1-like ATPase family protein contain... 51 4e-07 At1g08820.1 68414.m00982 vesicle-associated membrane family prot... 37 0.009 At5g58830.1 68418.m07372 subtilase family protein contains simil... 29 1.4 At1g12150.1 68414.m01407 expressed protein contains Pfam profile... 28 3.3 At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein le... 27 5.8 At2g20010.1 68415.m02339 expressed protein 27 5.8 At1g60110.1 68414.m06772 jacalin lectin family protein similar t... 27 5.8 At4g24520.1 68417.m03515 NADPH-cytochrome p450 reductase, putati... 27 7.7 At3g55770.1 68416.m06197 LIM domain-containing protein similar t... 27 7.7 At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative si... 27 7.7 >At2g25530.1 68415.m03056 AFG1-like ATPase family protein contains Pfam profile: PF03969 AFG1-like ATPase Length = 655 Score = 60.1 bits (139), Expect = 9e-10 Identities = 43/128 (33%), Positives = 71/128 (55%), Gaps = 16/128 (12%) Frame = +3 Query: 6 ISKTFHTVFIRNLPQLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMK----- 170 ++K +HT+FI ++P +S+ + R + RRFITL+D LY++ +V +A++ L + Sbjct: 516 VAKNYHTIFISDIPAMSM-EIRDKARRFITLVDELYNHHCCLVSSAETAIDELFQGTAEG 574 Query: 171 --LD--------ETEFGDADRALMDDLKIT-KDSEDAKATIFTGEEEMFACDRCLSRIME 317 D ETE R ++ + I+ S + ++ +GEEEMFA R SR++E Sbjct: 575 TLFDLESFQFETETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARAASRLIE 634 Query: 318 MQTDEYWE 341 MQT Y E Sbjct: 635 MQTPLYLE 642 >At4g30490.1 68417.m04329 AFG1-like ATPase family protein contains Pfam profile: PF03969 AFG1-like ATPase Length = 497 Score = 57.2 bits (132), Expect = 6e-09 Identities = 32/110 (29%), Positives = 58/110 (52%) Frame = +3 Query: 12 KTFHTVFIRNLPQLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFG 191 K FHT+ + +P + +R+ RF+TL+D +Y+NR R++ A++ P+ L+ E Sbjct: 384 KKFHTLALDEIPVFGLH-NRTAAYRFVTLVDVMYENRARLLCTAEANPQELL---EKIIT 439 Query: 192 DADRALMDDLKITKDSEDAKATIFTGEEEMFACDRCLSRIMEMQTDEYWE 341 ++ M ++ ++ + E FA DR +SR+ EM + EY E Sbjct: 440 ISEAKSMGPRTSSRSRKNDVTELCVDNELGFAKDRTISRLTEMNSKEYLE 489 >At4g28070.1 68417.m04026 AFG1-like ATPase family protein contains Pfam profile: PF03969 AFG1-like ATPase Length = 464 Score = 51.2 bits (117), Expect = 4e-07 Identities = 29/93 (31%), Positives = 50/93 (53%) Frame = +3 Query: 66 HRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITKDSED 245 +R+ RF+TL+D +Y+N+ R++ A+ P L++ T DA + ++ S+D Sbjct: 363 NRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVT-ISDAQQIAPRTSSRSRKSDD 421 Query: 246 AKATIFTGEEEMFACDRCLSRIMEMQTDEYWEK 344 + E FA DR +SR+ EM + EY E+ Sbjct: 422 PDLCV--DNELGFAKDRTISRLTEMNSKEYLEQ 452 >At1g08820.1 68414.m00982 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 386 Score = 36.7 bits (81), Expect = 0.009 Identities = 24/66 (36%), Positives = 39/66 (59%), Gaps = 5/66 (7%) Frame = +3 Query: 135 IAADSEPK-----NLMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEEMFACDRC 299 IA DSEPK ++++L +T+ G R L D+LK+ KD E+ K + E ++ D Sbjct: 270 IAVDSEPKISKERDVVQLQKTD-GQNVRGL-DELKLVKDIEEMKLKVDALESKLKQADST 327 Query: 300 LSRIME 317 +S++ME Sbjct: 328 ISKLME 333 >At5g58830.1 68418.m07372 subtilase family protein contains similarity to prepro-cucumisin GI:807698 from [Cucumis melo] Length = 671 Score = 29.5 bits (63), Expect = 1.4 Identities = 14/46 (30%), Positives = 27/46 (58%) Frame = -2 Query: 358 ICVPHFSQYSSVCISIIRERHRSHANISSSPVNIVALASSESLVIF 221 I V + S V +S I ++ +A+ISS P+++++ +SLV + Sbjct: 333 ILVSRYLSGSEVAVSFITTDNKDYASISSRPLSVLSQDDFDSLVSY 378 >At1g12150.1 68414.m01407 expressed protein contains Pfam profile PF05701: Plant protein of unknown function (DUF827); expression supported by MPSS Length = 548 Score = 28.3 bits (60), Expect = 3.3 Identities = 20/66 (30%), Positives = 31/66 (46%) Frame = +3 Query: 48 QLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKI 227 ++++SK RS RR +++ D ++++ K KLD E RAL D K Sbjct: 39 EVAVSKQRSTPRRSRLSSESVCDKETQLMLVHKEFMKIKQKLDNAE-STRSRALDDLSKA 97 Query: 228 TKDSED 245 K ED Sbjct: 98 KKTMED 103 >At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein leucine zipper-containing protein - Lycopersicon esculentum, EMBL:Z12127 contains Pfam domain PF03081: Exo70 exocyst complex subunit; Length = 653 Score = 27.5 bits (58), Expect = 5.8 Identities = 18/72 (25%), Positives = 40/72 (55%), Gaps = 1/72 (1%) Frame = +3 Query: 57 ISKHRSQ-LRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITK 233 ++K S+ L +F+ + +TL D + V +DS+ +KL +T G+A + +L+ + Sbjct: 331 LTKRSSEKLFKFLDMYETLRD-LIPAVEQSDSDLIQEIKLAQTRLGEAAVTIFGELEKSI 389 Query: 234 DSEDAKATIFTG 269 S++ + + +G Sbjct: 390 KSDNGRTPVPSG 401 >At2g20010.1 68415.m02339 expressed protein Length = 834 Score = 27.5 bits (58), Expect = 5.8 Identities = 13/47 (27%), Positives = 30/47 (63%) Frame = +3 Query: 60 SKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDAD 200 S + ++L++F++ I L + +RV+ AAD K+L+++ + D++ Sbjct: 374 SCYGTELKKFVSGITELTPDAIRVLTAADKLEKDLVQIAVQDAVDSE 420 >At1g60110.1 68414.m06772 jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI:2997767; contains Pfam profile PF01419 jacalin-like lectin domain Length = 598 Score = 27.5 bits (58), Expect = 5.8 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = +3 Query: 90 ITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALM 212 I + LYD +VV+ D K L+ +DE E D L+ Sbjct: 486 IAFVKFLYDKDNKVVVGDDHGSKTLLGVDEFELEHPDEYLI 526 >At4g24520.1 68417.m03515 NADPH-cytochrome p450 reductase, putative / NADPH-ferrihemoprotein reductase, putative similar to NADPH-ferrihemoprotein reductase NADPH-cytochrome P450 oxydoreductase isoform 1 [Populus balsamifera subsp. trichocarpa x Populus deltoides] GI:13183562, SP|P37116 NADPH-cytochrome P450 reductase (EC 1.6.2.4) (CPR) [Vigna radiata] {Phaseolus aureus} Length = 692 Score = 27.1 bits (57), Expect = 7.7 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +3 Query: 117 NRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITKDS 239 N++ +V+ + K +L E GD D+++ DD K+S Sbjct: 193 NKIGIVLDEELCKKGAKRLIEVGLGDDDQSIEDDFNAWKES 233 >At3g55770.1 68416.m06197 LIM domain-containing protein similar to pollen specific LIM domain protein 1b [Nicotiana tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida] GI:4105772; contains Pfam profile PF00412: LIM domain Length = 199 Score = 27.1 bits (57), Expect = 7.7 Identities = 11/33 (33%), Positives = 16/33 (48%) Frame = +1 Query: 382 YH*QCFSVTYLKINKNASTYLLKEYMIICKRQY 480 YH CF T+ K S+Y E ++ CK + Sbjct: 30 YHKSCFKCTHCKSRLQLSSYSSMEGVLYCKPHF 62 >At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative similar to SP|P47047 ATP-dependent RNA helicase DOB1 {Saccharomyces cerevisiae}, HUA enhancer 2 [Arabidopsis thaliana] GI:16024936; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 988 Score = 27.1 bits (57), Expect = 7.7 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +3 Query: 144 DSEPKNLMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEE 278 + +P+NL++ +F ADRA+ D K K E+ + ++ EEE Sbjct: 512 EGDPENLLRNSFFQF-QADRAIPDLEKQIKSLEEERDSLVIEEEE 555 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,853,105 Number of Sequences: 28952 Number of extensions: 190512 Number of successful extensions: 390 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 385 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 389 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 957410176 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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