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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS302F01f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g25530.1 68415.m03056 AFG1-like ATPase family protein contain...    60   9e-10
At4g30490.1 68417.m04329 AFG1-like ATPase family protein contain...    57   6e-09
At4g28070.1 68417.m04026 AFG1-like ATPase family protein contain...    51   4e-07
At1g08820.1 68414.m00982 vesicle-associated membrane family prot...    37   0.009
At5g58830.1 68418.m07372 subtilase family protein contains simil...    29   1.4  
At1g12150.1 68414.m01407 expressed protein contains Pfam profile...    28   3.3  
At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein le...    27   5.8  
At2g20010.1 68415.m02339 expressed protein                             27   5.8  
At1g60110.1 68414.m06772 jacalin lectin family protein similar t...    27   5.8  
At4g24520.1 68417.m03515 NADPH-cytochrome p450 reductase, putati...    27   7.7  
At3g55770.1 68416.m06197 LIM domain-containing protein similar t...    27   7.7  
At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative si...    27   7.7  

>At2g25530.1 68415.m03056 AFG1-like ATPase family protein contains
           Pfam profile: PF03969 AFG1-like ATPase
          Length = 655

 Score = 60.1 bits (139), Expect = 9e-10
 Identities = 43/128 (33%), Positives = 71/128 (55%), Gaps = 16/128 (12%)
 Frame = +3

Query: 6   ISKTFHTVFIRNLPQLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMK----- 170
           ++K +HT+FI ++P +S+ + R + RRFITL+D LY++   +V +A++    L +     
Sbjct: 516 VAKNYHTIFISDIPAMSM-EIRDKARRFITLVDELYNHHCCLVSSAETAIDELFQGTAEG 574

Query: 171 --LD--------ETEFGDADRALMDDLKIT-KDSEDAKATIFTGEEEMFACDRCLSRIME 317
              D        ETE     R ++ +  I+   S  +  ++ +GEEEMFA  R  SR++E
Sbjct: 575 TLFDLESFQFETETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARAASRLIE 634

Query: 318 MQTDEYWE 341
           MQT  Y E
Sbjct: 635 MQTPLYLE 642


>At4g30490.1 68417.m04329 AFG1-like ATPase family protein contains
           Pfam profile: PF03969 AFG1-like ATPase
          Length = 497

 Score = 57.2 bits (132), Expect = 6e-09
 Identities = 32/110 (29%), Positives = 58/110 (52%)
 Frame = +3

Query: 12  KTFHTVFIRNLPQLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFG 191
           K FHT+ +  +P   +  +R+   RF+TL+D +Y+NR R++  A++ P+ L+   E    
Sbjct: 384 KKFHTLALDEIPVFGLH-NRTAAYRFVTLVDVMYENRARLLCTAEANPQELL---EKIIT 439

Query: 192 DADRALMDDLKITKDSEDAKATIFTGEEEMFACDRCLSRIMEMQTDEYWE 341
            ++   M     ++  ++    +    E  FA DR +SR+ EM + EY E
Sbjct: 440 ISEAKSMGPRTSSRSRKNDVTELCVDNELGFAKDRTISRLTEMNSKEYLE 489


>At4g28070.1 68417.m04026 AFG1-like ATPase family protein contains
           Pfam profile: PF03969 AFG1-like ATPase
          Length = 464

 Score = 51.2 bits (117), Expect = 4e-07
 Identities = 29/93 (31%), Positives = 50/93 (53%)
 Frame = +3

Query: 66  HRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITKDSED 245
           +R+   RF+TL+D +Y+N+ R++  A+  P  L++   T   DA +        ++ S+D
Sbjct: 363 NRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVT-ISDAQQIAPRTSSRSRKSDD 421

Query: 246 AKATIFTGEEEMFACDRCLSRIMEMQTDEYWEK 344
               +    E  FA DR +SR+ EM + EY E+
Sbjct: 422 PDLCV--DNELGFAKDRTISRLTEMNSKEYLEQ 452


>At1g08820.1 68414.m00982 vesicle-associated membrane family protein
           / VAMP family protein similar to VAP27 GI:6688926
           [Nicotiana plumbaginifolia]
          Length = 386

 Score = 36.7 bits (81), Expect = 0.009
 Identities = 24/66 (36%), Positives = 39/66 (59%), Gaps = 5/66 (7%)
 Frame = +3

Query: 135 IAADSEPK-----NLMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEEMFACDRC 299
           IA DSEPK     ++++L +T+ G   R L D+LK+ KD E+ K  +   E ++   D  
Sbjct: 270 IAVDSEPKISKERDVVQLQKTD-GQNVRGL-DELKLVKDIEEMKLKVDALESKLKQADST 327

Query: 300 LSRIME 317
           +S++ME
Sbjct: 328 ISKLME 333


>At5g58830.1 68418.m07372 subtilase family protein contains
           similarity to prepro-cucumisin GI:807698 from [Cucumis
           melo]
          Length = 671

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 14/46 (30%), Positives = 27/46 (58%)
 Frame = -2

Query: 358 ICVPHFSQYSSVCISIIRERHRSHANISSSPVNIVALASSESLVIF 221
           I V  +   S V +S I   ++ +A+ISS P+++++    +SLV +
Sbjct: 333 ILVSRYLSGSEVAVSFITTDNKDYASISSRPLSVLSQDDFDSLVSY 378


>At1g12150.1 68414.m01407 expressed protein contains Pfam profile
           PF05701: Plant protein of unknown function (DUF827);
           expression supported by MPSS
          Length = 548

 Score = 28.3 bits (60), Expect = 3.3
 Identities = 20/66 (30%), Positives = 31/66 (46%)
 Frame = +3

Query: 48  QLSISKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKI 227
           ++++SK RS  RR     +++ D   ++++      K   KLD  E     RAL D  K 
Sbjct: 39  EVAVSKQRSTPRRSRLSSESVCDKETQLMLVHKEFMKIKQKLDNAE-STRSRALDDLSKA 97

Query: 228 TKDSED 245
            K  ED
Sbjct: 98  KKTMED 103


>At5g13150.1 68418.m01506 exocyst subunit EXO70 family protein
           leucine zipper-containing protein - Lycopersicon
           esculentum, EMBL:Z12127 contains Pfam domain PF03081:
           Exo70 exocyst complex subunit;
          Length = 653

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 18/72 (25%), Positives = 40/72 (55%), Gaps = 1/72 (1%)
 Frame = +3

Query: 57  ISKHRSQ-LRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITK 233
           ++K  S+ L +F+ + +TL D  +  V  +DS+    +KL +T  G+A   +  +L+ + 
Sbjct: 331 LTKRSSEKLFKFLDMYETLRD-LIPAVEQSDSDLIQEIKLAQTRLGEAAVTIFGELEKSI 389

Query: 234 DSEDAKATIFTG 269
            S++ +  + +G
Sbjct: 390 KSDNGRTPVPSG 401


>At2g20010.1 68415.m02339 expressed protein
          Length = 834

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 13/47 (27%), Positives = 30/47 (63%)
 Frame = +3

Query: 60  SKHRSQLRRFITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDAD 200
           S + ++L++F++ I  L  + +RV+ AAD   K+L+++   +  D++
Sbjct: 374 SCYGTELKKFVSGITELTPDAIRVLTAADKLEKDLVQIAVQDAVDSE 420


>At1g60110.1 68414.m06772 jacalin lectin family protein similar to
           myrosinase-binding protein homolog [Arabidopsis
           thaliana] GI:2997767; contains Pfam profile PF01419
           jacalin-like lectin domain
          Length = 598

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = +3

Query: 90  ITLIDTLYDNRVRVVIAADSEPKNLMKLDETEFGDADRALM 212
           I  +  LYD   +VV+  D   K L+ +DE E    D  L+
Sbjct: 486 IAFVKFLYDKDNKVVVGDDHGSKTLLGVDEFELEHPDEYLI 526


>At4g24520.1 68417.m03515 NADPH-cytochrome p450 reductase, putative
           / NADPH-ferrihemoprotein reductase, putative similar to
           NADPH-ferrihemoprotein reductase NADPH-cytochrome P450
           oxydoreductase isoform 1 [Populus balsamifera subsp.
           trichocarpa x Populus deltoides] GI:13183562, SP|P37116
           NADPH-cytochrome P450 reductase (EC 1.6.2.4) (CPR)
           [Vigna radiata] {Phaseolus aureus}
          Length = 692

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = +3

Query: 117 NRVRVVIAADSEPKNLMKLDETEFGDADRALMDDLKITKDS 239
           N++ +V+  +   K   +L E   GD D+++ DD    K+S
Sbjct: 193 NKIGIVLDEELCKKGAKRLIEVGLGDDDQSIEDDFNAWKES 233


>At3g55770.1 68416.m06197 LIM domain-containing protein similar to
           pollen specific LIM domain protein 1b [Nicotiana
           tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
           GI:4105772; contains Pfam profile PF00412: LIM domain
          Length = 199

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/33 (33%), Positives = 16/33 (48%)
 Frame = +1

Query: 382 YH*QCFSVTYLKINKNASTYLLKEYMIICKRQY 480
           YH  CF  T+ K     S+Y   E ++ CK  +
Sbjct: 30  YHKSCFKCTHCKSRLQLSSYSSMEGVLYCKPHF 62


>At1g59760.1 68414.m06729 ATP-dependent RNA helicase, putative
           similar to SP|P47047 ATP-dependent RNA helicase DOB1
           {Saccharomyces cerevisiae}, HUA enhancer 2 [Arabidopsis
           thaliana] GI:16024936; contains Pfam profiles PF00270:
           DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
          Length = 988

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = +3

Query: 144 DSEPKNLMKLDETEFGDADRALMDDLKITKDSEDAKATIFTGEEE 278
           + +P+NL++    +F  ADRA+ D  K  K  E+ + ++   EEE
Sbjct: 512 EGDPENLLRNSFFQF-QADRAIPDLEKQIKSLEEERDSLVIEEEE 555


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,853,105
Number of Sequences: 28952
Number of extensions: 190512
Number of successful extensions: 390
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 385
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 389
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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