Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302E06f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 40 0.001
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 38 0.005
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 37 0.009
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 37 0.009
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 36 0.012
At5g55020.1 68418.m06853 myb family transcription factor (MYB120... 31 0.47
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 31 0.47
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 31 0.47
At1g50950.1 68414.m05728 thioredoxin-related contains weak hit t... 30 0.82
At3g23330.1 68416.m02943 pentatricopeptide (PPR) repeat-containi... 29 1.9
At5g51070.1 68418.m06330 ATP-dependent Clp protease ATP-binding ... 29 2.5
At4g18610.1 68417.m02756 expressed protein contains Pfam profile... 29 2.5
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF... 27 5.8
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 27 7.7
At3g13810.1 68416.m01744 zinc finger (C2H2 type) family protein ... 27 7.7
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 39.5 bits (88), Expect = 0.001
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +1
Query: 1 KDGKLQPQEYQGGTVTVSNLGMY 69
+ GKL P E+QGGT ++SNLGMY
Sbjct: 536 RSGKLAPHEFQGGTFSISNLGMY 558
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 37.5 bits (83), Expect = 0.005
Identities = 15/22 (68%), Positives = 19/22 (86%)
Frame = +1
Query: 10 KLQPQEYQGGTVTVSNLGMYGM 75
+LQPQEY GT T+SNLGM+G+
Sbjct: 371 QLQPQEYNTGTFTLSNLGMFGV 392
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 36.7 bits (81), Expect = 0.009
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +1
Query: 1 KDGKLQPQEYQGGTVTVSNLGMYGMYSLSTFSCV 102
K+ L+P++Y+GGT TVSNLG G + + F V
Sbjct: 440 KENSLKPEDYEGGTFTVSNLG--GPFGIKQFCAV 471
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 36.7 bits (81), Expect = 0.009
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +1
Query: 1 KDGKLQPQEYQGGTVTVSNLGMYGMYSLSTFSCV 102
K+ L+P++Y+GGT TVSNLG G + + F V
Sbjct: 440 KENSLKPEDYEGGTFTVSNLG--GPFGIKQFCAV 471
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 36.3 bits (80), Expect = 0.012
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +1
Query: 10 KLQPQEYQGGTVTVSNLGMYGM 75
+LQP EY GT T+SNLGM+G+
Sbjct: 386 QLQPHEYNSGTFTLSNLGMFGV 407
>At5g55020.1 68418.m06853 myb family transcription factor (MYB120)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 523
Score = 31.1 bits (67), Expect = 0.47
Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = -1
Query: 377 TADFSLPFSSISRHLNTHSSLSHIVIHTLHPCLLR---STSSPSTLH*HFHTSPSQSHAS 207
T + P + + + NT++ S +L+P STSSPS LH H+ S + H +
Sbjct: 323 TLPYPSPTAQTATYHNTNNPYSSSPSFSLNPSSSSYPTSTSSPSFLHSHYTPSSTSFHTN 382
Query: 206 SLYA 195
+Y+
Sbjct: 383 PVYS 386
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 31.1 bits (67), Expect = 0.47
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 4 DGKLQPQEYQGGTVTVSNLGMYG 72
+ KL P++ GGT+T+SN+G G
Sbjct: 384 NNKLNPEDVTGGTITLSNIGAIG 406
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 31.1 bits (67), Expect = 0.47
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 4 DGKLQPQEYQGGTVTVSNLGMYG 72
+ KL P++ GGT+T+SN+G G
Sbjct: 384 NNKLNPEDVTGGTITLSNIGAIG 406
>At1g50950.1 68414.m05728 thioredoxin-related contains weak hit to
Pfam PF00085: Thioredoxin; contains 2 predicted
transmembrane domains
Length = 484
Score = 30.3 bits (65), Expect = 0.82
Identities = 21/70 (30%), Positives = 27/70 (38%)
Frame = -1
Query: 347 ISRHLNTHSSLSHIVIHTLHPCLLRSTSSPSTLH*HFHTSPSQSHASSLYASHDHTTLSV 168
ISR SL +T H + RS P HF SP Q S S H +V
Sbjct: 392 ISRRSGQEHSLIEEYEYTAHSSVARSYHYPEAKF-HFELSPMQVLISENPKSFSHFITNV 450
Query: 167 CELFSIIFII 138
C + +F +
Sbjct: 451 CAIIGGVFTV 460
>At3g23330.1 68416.m02943 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 679
Score = 29.1 bits (62), Expect = 1.9
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
Frame = -1
Query: 320 SLSHIVIHTL--HPCLLRSTSSPSTLH*HFHTSPSQSHASSLYASHDHTTLSVCELFSII 147
S S +I TL +P ++S S LH F + S SH S+ +T L + ++
Sbjct: 2 SSSKALIKTLIKNPTRIKSKSQAKQLHAQFIRTQSLSHTSASIVISIYTNLKLLHEALLL 61
Query: 146 FIIVKTP 126
F +K+P
Sbjct: 62 FKTLKSP 68
>At5g51070.1 68418.m06330 ATP-dependent Clp protease ATP-binding
subunit (ClpD), (ERD1) SAG15/ERD1; identical to ERD1
protein GI:497629, SP:P42762 from [Arabidopsis
thaliana]; contains Pfam profile PF02861: Clp amino
terminal domain
Length = 945
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 2/29 (6%)
Frame = -1
Query: 272 STSSPSTLH*H--FHTSPSQSHASSLYAS 192
STSSP TLH H S S SH +S+ AS
Sbjct: 5 STSSPLTLHSHRLLSASSSSSHVTSIAAS 33
>At4g18610.1 68417.m02756 expressed protein contains Pfam profile
PF04852: Protein of unknown function (DUF640)
Length = 191
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -2
Query: 295 HSIHVFFGRPLPPLPCT 245
H VFFG+P PP PCT
Sbjct: 87 HQACVFFGQPDPPGPCT 103
>At1g35830.1 68414.m04452 VQ motif-containing protein contains
PF05678: VQ motif
Length = 302
Score = 27.5 bits (58), Expect = 5.8
Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
Frame = -1
Query: 386 GVGTADFSLPFSSISRHLNTHSSLSHIVIHTLHPCLLRSTSSPST---LH*HFHTSPSQS 216
GV + FS PFSS +R + S S + + + + +PST L+ H HT+ S +
Sbjct: 130 GVPASPFSHPFSSTTRRFDIFRSPSDPLTYNPFRPIPQKPLNPSTSSLLNLHHHTTTSMT 189
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 27.1 bits (57), Expect = 7.7
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 4 DGKLQPQEYQGGTVTVSNLGMYGMYSLSTFSCVNI*HLVLDGVLTIIK 147
+G + E GG+ TVSN G+YG SL + +N + G+ +I++
Sbjct: 367 EGTISIDEMAGGSFTVSNGGVYG--SLISTPIINPPQSAILGMHSIVQ 412
>At3g13810.1 68416.m01744 zinc finger (C2H2 type) family protein
similar to finger protein pcp1 GB:S48856 from [Solanum
tuberosum] contains Pfam domain, PF00096: Zinc finger,
C2H2 type
Length = 513
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/88 (21%), Positives = 40/88 (45%), Gaps = 1/88 (1%)
Frame = -1
Query: 290 HPCLLRSTSSPSTLH*HFHTSPSQSHASSLYASHDHTTLSVCELFSIIFIIVKTPSKTRC 111
+P L+ ++S H H T P+ + +SS +SH+H ++ F + +
Sbjct: 247 NPLLIHQSASHP--HHHHQTQPTINVSSSSSSSHNHNIINSLH-FDTNNGNTNNSNNSNN 303
Query: 110 HMFT-QLNVDKEYIPYIPRFDTVTVPPW 30
H+ T + +++ +I + +PPW
Sbjct: 304 HLHTFPMKKEQQSNDHIMNYHHSIIPPW 331
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,057,354
Number of Sequences: 28952
Number of extensions: 216528
Number of successful extensions: 535
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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