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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS302E04f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ...    94   4e-20
At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase f...    41   4e-04
At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase f...    38   0.003
At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family pro...    28   4.4  
At3g59780.1 68416.m06671 expressed protein                             27   7.7  
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup...    27   7.7  
At1g35610.1 68414.m04421 DC1 domain-containing protein contains ...    27   7.7  

>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
           putative / ubiquitin thiolesterase, putative similar to
           SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
           L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
           {Mus musculus}; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 234

 Score = 94.3 bits (224), Expect = 4e-20
 Identities = 47/106 (44%), Positives = 69/106 (65%), Gaps = 1/106 (0%)
 Frame = +3

Query: 207 VPLESNPDVLNKFLQKLGV-PNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENH 383
           +PLESNPDV+N++L  LG+ P++    DV GLD E L  VP+PVL+V+ L+PI+   E  
Sbjct: 14  LPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLAVLFLYPITKKSEEE 73

Query: 384 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVXXNTDII 521
           +  ++ EI  K    S  +++MKQ + NACGTI L+H++   T  I
Sbjct: 74  RIEQDKEIKEKVH--SDKVYFMKQTVGNACGTIGLLHAIGNITSEI 117


>At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 334

 Score = 41.1 bits (92), Expect = 4e-04
 Identities = 31/105 (29%), Positives = 57/105 (54%)
 Frame = +3

Query: 207 VPLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHK 386
           +P+ES+P +  + +Q++ V     + ++  LD  +L  + RPV  ++LL      Y+   
Sbjct: 4   LPVESDPGIFTEIIQQMQVKGV-QVEELYSLDFNSLDEI-RPVYGLILL------YKWRP 55

Query: 387 KTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVXXNTDII 521
           + +EN ++    E + N F+  Q I+NAC T A++ SV  N+  I
Sbjct: 56  EEKENRVVIT--EPNPNFFFASQIINNACATQAIL-SVLMNSSSI 97


>At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 330

 Score = 38.3 bits (85), Expect = 0.003
 Identities = 24/93 (25%), Positives = 51/93 (54%)
 Frame = +3

Query: 213 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 392
           +ES+P V  + +Q++ V     + ++  LD ++L+ + RPV  ++ LF      ++ + T
Sbjct: 6   IESDPGVFTELIQQMQVKGV-QVEELYSLDSDSLNNL-RPVYGLIFLFKWQAGEKDERPT 63

Query: 393 EENEILSKGQEVSGNIFYMKQNISNACGTIALV 491
            ++++         N+F+  Q I+NAC T A++
Sbjct: 64  IQDQV--------SNLFFANQVINNACATQAIL 88


>At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family
           protein contains Pfam domain, PF05183: RNA dependent RNA
           polymerase
          Length = 992

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 13/55 (23%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = -2

Query: 355 KSSITESTGRGTHESVSGSSPMTSTIFHLFGTPSFCKNLFRT-SGLDSRGTKVSV 194
           K  + E+TG   H+ +   + +T     + G  ++C +L+ T  G+   G  V +
Sbjct: 229 KGHLLENTGTHLHKVLGDDNVLTVKFDKVLGVETYCNDLYSTYKGIAKNGIMVGL 283


>At3g59780.1 68416.m06671 expressed protein 
          Length = 610

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 8/15 (53%), Positives = 12/15 (80%)
 Frame = -3

Query: 234 GHRDWIQEGLKFRSP 190
           G+R W+QEGL+ + P
Sbjct: 440 GYRSWVQEGLRVKEP 454


>At2g30870.1 68415.m03762 glutathione S-transferase, putative
           supported by cDNA GI:443698 GB:D17673
          Length = 215

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 14/62 (22%), Positives = 28/62 (45%)
 Frame = +3

Query: 225 PDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKTEENE 404
           PD+L K +++ G   +W  V+     P  L+     V + ++ FP  +      + +  E
Sbjct: 82  PDLLGKTIEERGQVEQWLDVEATSYHPPLLALTLNIVFAPLMGFPADEKVIKESEEKLAE 141

Query: 405 IL 410
           +L
Sbjct: 142 VL 143


>At1g35610.1 68414.m04421 DC1 domain-containing protein contains
           Pfam protein PF03107 DC1 domain
          Length = 612

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 10/21 (47%), Positives = 14/21 (66%)
 Frame = +1

Query: 94  VFNSIVNLRRYFFICHCRCTG 156
           VF +  N R+  ++CH RCTG
Sbjct: 545 VFRNNSNTRQLCYMCHNRCTG 565


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,155,812
Number of Sequences: 28952
Number of extensions: 223687
Number of successful extensions: 481
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 471
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 478
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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