BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS302D12f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 196 1e-51 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 93 3e-20 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 92 4e-20 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 87 1e-18 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 73 2e-14 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 60 3e-10 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 47 1e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 47 1e-06 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 1e-05 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 0.97 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 3.9 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 6.8 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 6.8 SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 6.8 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 6.8 SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 25 9.0 SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 25 9.0 SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 25 9.0 SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 25 9.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 196 bits (479), Expect = 1e-51 Identities = 89/98 (90%), Positives = 94/98 (95%) Frame = -2 Query: 517 TYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKY 338 TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKY Sbjct: 278 TYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKY 337 Query: 337 SVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 224 SVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF Sbjct: 338 SVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 92.7 bits (220), Expect = 3e-20 Identities = 45/101 (44%), Positives = 68/101 (67%), Gaps = 6/101 (5%) Frame = -2 Query: 511 NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERK 341 N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A ER Sbjct: 333 NCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERS 391 Query: 340 YSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 227 Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C Sbjct: 392 YASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 92.3 bits (219), Expect = 4e-20 Identities = 41/97 (42%), Positives = 68/97 (70%) Frame = -2 Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYS 335 + SI+ D+D+R LY+N VLSGG+T+ G +R E+ A++ ++KI A PER ++ Sbjct: 284 FKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHN 343 Query: 334 VWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 224 W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F Sbjct: 344 AWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 87.0 bits (206), Expect = 1e-18 Identities = 41/90 (45%), Positives = 58/90 (64%), Gaps = 2/90 (2%) Frame = -2 Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKIIAPPER-K 341 Y SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S + + Sbjct: 329 YQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATS 388 Query: 340 YSVWIGGSILASLSTFQQMWISKQEYDESG 251 +VW GGSILASL FQ +W+SKQEYDE G Sbjct: 389 NAVWFGGSILASLDNFQHLWVSKQEYDEVG 418 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 73.3 bits (172), Expect = 2e-14 Identities = 41/100 (41%), Positives = 63/100 (63%), Gaps = 12/100 (12%) Frame = -2 Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APSTM---KI 368 +++I DVDIRK LY VLSGG++MY G+ R++KEI L P+ + K+ Sbjct: 279 FDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKV 338 Query: 367 KIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 251 KI P R+++V+IGG++LA ++ MW+SK E++E G Sbjct: 339 KIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 59.7 bits (138), Expect = 3e-10 Identities = 31/107 (28%), Positives = 54/107 (50%), Gaps = 14/107 (13%) Frame = -2 Query: 511 NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST--------------M 374 N + +D+RK LY N VLSGG+T++ +R+Q+++ + + Sbjct: 313 NVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGV 372 Query: 373 KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 233 + +I+ ++ +VW GGS+LA F +K +Y+E G SI R Sbjct: 373 DVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 47.2 bits (107), Expect = 1e-06 Identities = 26/86 (30%), Positives = 43/86 (50%) Frame = -2 Query: 487 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILA 308 +I+ L N V GG +PG R+ E+ +LAP+ ++K+ P + W S + Sbjct: 314 NIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM- 372 Query: 307 SLSTFQQMWISKQEYDESGPSIVHRK 230 L + I++ EY E G +I+ RK Sbjct: 373 PLEHWNANKITRSEYSEHGANIMTRK 398 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 47.2 bits (107), Expect = 1e-06 Identities = 20/89 (22%), Positives = 45/89 (50%) Frame = -2 Query: 505 IMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI 326 + + ++ ++ L +N +++GG PG+ R+++E+T++ P I + W Sbjct: 623 LQRHSLEEQQKLVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWK 682 Query: 325 GGSILASLSTFQQMWISKQEYDESGPSIV 239 G S + F+ ++++EY E GP + Sbjct: 683 GASEWSVTEKFKAAKVTREEYLEKGPEYI 711 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 44.4 bits (100), Expect = 1e-05 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%) Frame = -2 Query: 478 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 308 K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586 Query: 307 SLSTFQQMWISKQEYDESGPSIVHRK 230 + ++WI ++ G ++ K Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 0.97 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = -2 Query: 331 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 230 ++GGSI+A S + + +++ +EY + GP+ +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 25.8 bits (54), Expect = 3.9 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = -2 Query: 406 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 296 KEI++ A S + + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.0 bits (52), Expect = 6.8 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -3 Query: 339 TPYGSVDRSSPPSLPSNKCGSRNKSTTSLAPP 244 TP + PPS+PS++ R S ++ APP Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = -3 Query: 333 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 247 Y + SSPP P N G + N +TTS P Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643 >SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1284 Score = 25.0 bits (52), Expect = 6.8 Identities = 11/29 (37%), Positives = 15/29 (51%) Frame = +2 Query: 272 FRDPHLLEGREGGEDRSTDPYGVLPLWRS 358 F HLL + G+D + + YG L W S Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = -3 Query: 333 YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 247 Y + SSPP P N G + N +TTS P Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643 >SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -3 Query: 300 LPSNKCGSRNKSTTSLAPPLYTGSAS 223 +P + STT + PPL T S+S Sbjct: 31 IPPGSLSENDNSTTFIKPPLETASSS 56 >SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 2280 Score = 24.6 bits (51), Expect = 9.0 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = -2 Query: 304 LSTFQQMWISKQEYDESGPSIV 239 L + + W KQ++DES S+V Sbjct: 2195 LQKWYEEWCGKQDWDESDKSVV 2216 >SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 24.6 bits (51), Expect = 9.0 Identities = 9/17 (52%), Positives = 12/17 (70%) Frame = +2 Query: 350 WRSNDLNLHCRWGESCD 400 +RS ++ L CRWG S D Sbjct: 380 YRSPEVILGCRWGASAD 396 >SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 204 Score = 24.6 bits (51), Expect = 9.0 Identities = 19/56 (33%), Positives = 23/56 (41%) Frame = -3 Query: 336 PYGSVDRSSPPSLPSNKCGSRNKSTTSLAPPLYTGSASKRTARRCLQQPAAGCSIQ 169 P SVD S+PPS P S + S P SA+ A Q A G + Q Sbjct: 49 PPPSVDHSAPPSGPP---PSYSNSAAPATPAASASSAAPAPAPAASQNRAYGAAPQ 101 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,041,200 Number of Sequences: 5004 Number of extensions: 38637 Number of successful extensions: 125 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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