SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS302D12f
         (521 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   196   1e-51
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    93   3e-20
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...    92   4e-20
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    87   1e-18
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...    73   2e-14
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    60   3e-10
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    47   1e-06
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    47   1e-06
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    44   1e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    28   0.97 
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha...    26   3.9  
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    25   6.8  
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom...    25   6.8  
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc...    25   6.8  
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom...    25   6.8  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    25   9.0  
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p...    25   9.0  
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar...    25   9.0  
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom...    25   9.0  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  196 bits (479), Expect = 1e-51
 Identities = 89/98 (90%), Positives = 94/98 (95%)
 Frame = -2

Query: 517 TYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKY 338
           TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKY
Sbjct: 278 TYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKY 337

Query: 337 SVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 224
           SVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 338 SVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 92.7 bits (220), Expect = 3e-20
 Identities = 45/101 (44%), Positives = 68/101 (67%), Gaps = 6/101 (5%)
 Frame = -2

Query: 511 NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERK 341
           N I +CDVDIR  L  N ++ GGT++  G + R+Q E++ L P + ++KI A     ER 
Sbjct: 333 NCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERS 391

Query: 340 YSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 227
           Y+ W+GGSIL+SL TF Q+WIS+QEY+E G    +++ ++C
Sbjct: 392 YASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score = 92.3 bits (219), Expect = 4e-20
 Identities = 41/97 (42%), Positives = 68/97 (70%)
 Frame = -2

Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYS 335
           + SI+  D+D+R  LY+N VLSGG+T+  G  +R   E+ A++    ++KI A PER ++
Sbjct: 284 FKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHN 343

Query: 334 VWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 224
            W+GGSILASLSTF+++ I+ +EY ++  +++ R+ F
Sbjct: 344 AWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 87.0 bits (206), Expect = 1e-18
 Identities = 41/90 (45%), Positives = 58/90 (64%), Gaps = 2/90 (2%)
 Frame = -2

Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKIIAPPER-K 341
           Y SI+ CD ++R  L  N V++GGT++ PG+++R+Q E+  LA  S + +          
Sbjct: 329 YQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATS 388

Query: 340 YSVWIGGSILASLSTFQQMWISKQEYDESG 251
            +VW GGSILASL  FQ +W+SKQEYDE G
Sbjct: 389 NAVWFGGSILASLDNFQHLWVSKQEYDEVG 418


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 73.3 bits (172), Expect = 2e-14
 Identities = 41/100 (41%), Positives = 63/100 (63%), Gaps = 12/100 (12%)
 Frame = -2

Query: 514 YNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APSTM---KI 368
           +++I   DVDIRK LY   VLSGG++MY G+  R++KEI  L         P+ +   K+
Sbjct: 279 FDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKV 338

Query: 367 KIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 251
           KI   P R+++V+IGG++LA  ++    MW+SK E++E G
Sbjct: 339 KIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 59.7 bits (138), Expect = 3e-10
 Identities = 31/107 (28%), Positives = 54/107 (50%), Gaps = 14/107 (13%)
 Frame = -2

Query: 511 NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST--------------M 374
           N +    +D+RK LY N VLSGG+T++    +R+Q+++  +                  +
Sbjct: 313 NVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGV 372

Query: 373 KIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 233
            + +I+   ++ +VW GGS+LA    F     +K +Y+E G SI  R
Sbjct: 373 DVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 47.2 bits (107), Expect = 1e-06
 Identities = 26/86 (30%), Positives = 43/86 (50%)
 Frame = -2

Query: 487 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILA 308
           +I+  L  N V  GG   +PG   R+  E+ +LAP+  ++K+  P +     W   S + 
Sbjct: 314 NIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM- 372

Query: 307 SLSTFQQMWISKQEYDESGPSIVHRK 230
            L  +    I++ EY E G +I+ RK
Sbjct: 373 PLEHWNANKITRSEYSEHGANIMTRK 398


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 47.2 bits (107), Expect = 1e-06
 Identities = 20/89 (22%), Positives = 45/89 (50%)
 Frame = -2

Query: 505 IMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI 326
           + +  ++ ++ L +N +++GG    PG+  R+++E+T++ P    I +          W 
Sbjct: 623 LQRHSLEEQQKLVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWK 682

Query: 325 GGSILASLSTFQQMWISKQEYDESGPSIV 239
           G S  +    F+   ++++EY E GP  +
Sbjct: 683 GASEWSVTEKFKAAKVTREEYLEKGPEYI 711


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
 Frame = -2

Query: 478 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 308
           K+LY++ ++ GG   +PG A  +++ I +   +   I +I PP   + ++  W G  I  
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586

Query: 307 SLSTFQQMWISKQEYDESGPSIVHRK 230
            +    ++WI   ++   G  ++  K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 27.9 bits (59), Expect = 0.97
 Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
 Frame = -2

Query: 331 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 230
           ++GGSI+A  S  + +   +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522


>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 617

 Score = 25.8 bits (54), Expect = 3.9
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -2

Query: 406 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 296
           KEI++ A S + +  + P E+    W    ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 339 TPYGSVDRSSPPSLPSNKCGSRNKSTTSLAPP 244
           TP  +     PPS+PS++   R  S ++ APP
Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254


>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
            pombe|chr 1||Partial|Manual
          Length = 1887

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
 Frame = -3

Query: 333  YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 247
            Y +   SSPP  P N  G  + N +TTS  P
Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643


>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1284

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = +2

Query: 272 FRDPHLLEGREGGEDRSTDPYGVLPLWRS 358
           F   HLL   + G+D + + YG L  W S
Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSWIS 825


>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1919

 Score = 25.0 bits (52), Expect = 6.8
 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
 Frame = -3

Query: 333  YGSVDRSSPPSLPSNKCG--SRNKSTTSLAP 247
            Y +   SSPP  P N  G  + N +TTS  P
Sbjct: 1613 YNASFSSSPPPQPGNSSGMSAMNTNTTSTTP 1643


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 10/26 (38%), Positives = 14/26 (53%)
 Frame = -3

Query: 300 LPSNKCGSRNKSTTSLAPPLYTGSAS 223
           +P       + STT + PPL T S+S
Sbjct: 31  IPPGSLSENDNSTTFIKPPLETASSS 56


>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2280

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -2

Query: 304  LSTFQQMWISKQEYDESGPSIV 239
            L  + + W  KQ++DES  S+V
Sbjct: 2195 LQKWYEEWCGKQDWDESDKSVV 2216


>SPBC530.14c |dsk1||SR protein-specific kinase
           Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 544

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = +2

Query: 350 WRSNDLNLHCRWGESCD 400
           +RS ++ L CRWG S D
Sbjct: 380 YRSPEVILGCRWGASAD 396


>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 204

 Score = 24.6 bits (51), Expect = 9.0
 Identities = 19/56 (33%), Positives = 23/56 (41%)
 Frame = -3

Query: 336 PYGSVDRSSPPSLPSNKCGSRNKSTTSLAPPLYTGSASKRTARRCLQQPAAGCSIQ 169
           P  SVD S+PPS P     S + S     P     SA+   A    Q  A G + Q
Sbjct: 49  PPPSVDHSAPPSGPP---PSYSNSAAPATPAASASSAAPAPAPAASQNRAYGAAPQ 101


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,041,200
Number of Sequences: 5004
Number of extensions: 38637
Number of successful extensions: 125
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -