Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302D07f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase, cytop... 167 5e-42
At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putat... 116 1e-26
At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putat... 116 1e-26
At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putat... 105 2e-23
At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putat... 105 2e-23
At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase, mitoc... 98 4e-21
At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase, mitoc... 98 4e-21
At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putat... 47 9e-06
At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putat... 47 9e-06
At5g56620.1 68418.m07069 no apical meristem (NAM) family protein... 33 0.15
At1g08840.1 68414.m00984 DNA replication helicase, putative simi... 31 0.47
At4g19900.1 68417.m02916 glycosyl transferase-related contains P... 29 1.9
At5g59100.1 68418.m07404 subtilase family protein contains simil... 28 3.3
At2g48050.1 68415.m06014 expressed protein ; expression supporte... 27 5.8
At1g17370.1 68414.m02118 oligouridylate-binding protein, putativ... 27 5.8
At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive eff... 27 5.8
At5g10650.1 68418.m01233 zinc finger (C3HC4-type RING finger) fa... 27 7.7
>At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase,
cytoplasmic / glyoxalase II (GLX2-2) identical to
SP|O24496 Hydroxyacylglutathione hydrolase cytoplasmic
(EC 3.1.2.6) (Glyoxalase II) (Glx II) {Arabidopsis
thaliana}
Length = 258
Score = 167 bits (405), Expect = 5e-42
Identities = 73/144 (50%), Positives = 104/144 (72%)
Frame = -3
Query: 519 GHICYFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCG 340
GHI Y+V +EG + VFTGDTLF+ GCG+FFEGTA+QMY++L L++LP T+V+CG
Sbjct: 115 GHISYYVNG-KEGENPAVFTGDTLFVAGCGKFFEGTAEQMYQSLCVTLAALPKPTQVYCG 173
Query: 339 HEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAV 160
HEYT++NL+FA VEP N ++ K++W++ +R PT+PST+ EE NPFMRV + +
Sbjct: 174 HEYTVKNLEFALTVEPNNGKIQQKLAWARQQRQADLPTIPSTLEEELETNPFMRVDKPEI 233
Query: 159 QNHTGKNDPIDTMKAIRLEKDTFK 88
Q G PIDTM+ +R +KD ++
Sbjct: 234 QEKLGCKSPIDTMREVRNKKDQWR 257
>At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 330
Score = 116 bits (279), Expect = 1e-26
Identities = 61/147 (41%), Positives = 87/147 (59%), Gaps = 3/147 (2%)
Frame = -3
Query: 519 GHICYFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCG 340
GHI ++ +FTGDTLF CG+ FEGT +QM +L I++ LPD T V+CG
Sbjct: 189 GHISFYFPGAR-----AIFTGDTLFSLSCGKLFEGTPEQMLASLQRIIA-LPDDTSVYCG 242
Query: 339 HEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAV 160
HEYTL N KFA +EPTNE +++ ++ + R++ PT+P+T+ EK NPF+R +
Sbjct: 243 HEYTLSNSKFALSIEPTNEVLQSYAAYVAELRDKKLPTIPTTMKMEKACNPFLRTENTDI 302
Query: 159 QNHTGKNDPIDTMKA---IRLEKDTFK 88
+ G + D +A IR KD FK
Sbjct: 303 RRALGIPETADEAEALGIIRRAKDNFK 329
>At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 331
Score = 116 bits (279), Expect = 1e-26
Identities = 61/147 (41%), Positives = 87/147 (59%), Gaps = 3/147 (2%)
Frame = -3
Query: 519 GHICYFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCG 340
GHI ++ +FTGDTLF CG+ FEGT +QM +L I++ LPD T V+CG
Sbjct: 190 GHISFYFPGAR-----AIFTGDTLFSLSCGKLFEGTPEQMLASLQRIIA-LPDDTSVYCG 243
Query: 339 HEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAV 160
HEYTL N KFA +EPTNE +++ ++ + R++ PT+P+T+ EK NPF+R +
Sbjct: 244 HEYTLSNSKFALSIEPTNEVLQSYAAYVAELRDKKLPTIPTTMKMEKACNPFLRTENTDI 303
Query: 159 QNHTGKNDPIDTMKA---IRLEKDTFK 88
+ G + D +A IR KD FK
Sbjct: 304 RRALGIPETADEAEALGIIRRAKDNFK 330
>At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 323
Score = 105 bits (251), Expect = 2e-23
Identities = 54/129 (41%), Positives = 78/129 (60%), Gaps = 3/129 (2%)
Frame = -3
Query: 468 VFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCGHEYTLQNLKFAAHVEPT 289
+FTGDT+F CG+ FEGT QM +L I +SLPD T ++CGHEYTL N KFA +EP
Sbjct: 196 IFTGDTMFSLSCGKLFEGTPKQMLASLQKI-TSLPDDTSIYCGHEYTLSNSKFALSLEPN 254
Query: 288 NENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAVQNHTGKNDPIDTMKA-- 115
NE +++ + + R++ PT+P+T+ EK NPF+R + ++ + D +A
Sbjct: 255 NEVLQSYAAHVAELRSKKLPTIPTTVKMEKACNPFLRSSNTDIRRALRIPEAADEAEALG 314
Query: 114 -IRLEKDTF 91
IR KD F
Sbjct: 315 IIRKAKDDF 323
>At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 324
Score = 105 bits (251), Expect = 2e-23
Identities = 54/129 (41%), Positives = 78/129 (60%), Gaps = 3/129 (2%)
Frame = -3
Query: 468 VFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCGHEYTLQNLKFAAHVEPT 289
+FTGDT+F CG+ FEGT QM +L I +SLPD T ++CGHEYTL N KFA +EP
Sbjct: 197 IFTGDTMFSLSCGKLFEGTPKQMLASLQKI-TSLPDDTSIYCGHEYTLSNSKFALSLEPN 255
Query: 288 NENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAVQNHTGKNDPIDTMKA-- 115
NE +++ + + R++ PT+P+T+ EK NPF+R + ++ + D +A
Sbjct: 256 NEVLQSYAAHVAELRSKKLPTIPTTVKMEKACNPFLRSSNTDIRRALRIPEAADEAEALG 315
Query: 114 -IRLEKDTF 91
IR KD F
Sbjct: 316 IIRKAKDDF 324
>At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 313
Score = 97.9 bits (233), Expect = 4e-21
Identities = 50/137 (36%), Positives = 77/137 (56%)
Frame = -3
Query: 519 GHICYFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCG 340
GHI ++ + +FTGD ++ CG EGT +QM +L I+S LPD T ++CG
Sbjct: 174 GHISFYFPG-----SATIFTGDLIYSLSCGTLSEGTPEQMLSSLQKIVS-LPDDTNIYCG 227
Query: 339 HEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAV 160
E T NLKFA VEP NE +++ + R+QG P++P+T+ EK NPF+R++ +
Sbjct: 228 RENTAGNLKFALSVEPKNETLQSYATRVAHLRSQGLPSIPTTVKVEKACNPFLRISSKDI 287
Query: 159 QNHTGKNDPIDTMKAIR 109
+ D +A+R
Sbjct: 288 RKSLSIPDSATEAEALR 304
>At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 331
Score = 97.9 bits (233), Expect = 4e-21
Identities = 50/137 (36%), Positives = 77/137 (56%)
Frame = -3
Query: 519 GHICYFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCG 340
GHI ++ + +FTGD ++ CG EGT +QM +L I+S LPD T ++CG
Sbjct: 192 GHISFYFPG-----SATIFTGDLIYSLSCGTLSEGTPEQMLSSLQKIVS-LPDDTNIYCG 245
Query: 339 HEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAV 160
E T NLKFA VEP NE +++ + R+QG P++P+T+ EK NPF+R++ +
Sbjct: 246 RENTAGNLKFALSVEPKNETLQSYATRVAHLRSQGLPSIPTTVKVEKACNPFLRISSKDI 305
Query: 159 QNHTGKNDPIDTMKAIR 109
+ D +A+R
Sbjct: 306 RKSLSIPDSATEAEALR 322
>At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 46.8 bits (106), Expect = 9e-06
Identities = 26/70 (37%), Positives = 41/70 (58%), Gaps = 7/70 (10%)
Frame = -3
Query: 519 GHICYFVT-APEEGNDS----VVFTGDTLFLGGCGR--FFEGTADQMYKALITILSSLPD 361
GH VT EG D + FTGD + + GCGR F EG++DQ+Y+++ + + +LP
Sbjct: 127 GHTAGCVTYVTGEGADQPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPK 186
Query: 360 HTKVFCGHEY 331
T ++ H+Y
Sbjct: 187 DTLIYPAHDY 196
>At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 46.8 bits (106), Expect = 9e-06
Identities = 26/70 (37%), Positives = 41/70 (58%), Gaps = 7/70 (10%)
Frame = -3
Query: 519 GHICYFVT-APEEGNDS----VVFTGDTLFLGGCGR--FFEGTADQMYKALITILSSLPD 361
GH VT EG D + FTGD + + GCGR F EG++DQ+Y+++ + + +LP
Sbjct: 127 GHTAGCVTYVTGEGADQPQPRMAFTGDAVLIRGCGRTDFQEGSSDQLYESVHSQIFTLPK 186
Query: 360 HTKVFCGHEY 331
T ++ H+Y
Sbjct: 187 DTLIYPAHDY 196
>At5g56620.1 68418.m07069 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
Length = 386
Score = 32.7 bits (71), Expect = 0.15
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 244 KPGKAHSAFYDR*REIIQSIHEGH*VGSSESHWQK 140
KP +A++ + R+IIQ+ H+ GSSE+ W K
Sbjct: 116 KPSRAYTTGTRKRRKIIQTDHDSELTGSSETRWHK 150
>At1g08840.1 68414.m00984 DNA replication helicase, putative similar
to helicase [Xenopus laevis] gi|18845092|gb|AAL79550
Length = 1296
Score = 31.1 bits (67), Expect = 0.47
Identities = 16/67 (23%), Positives = 30/67 (44%)
Frame = -3
Query: 294 PTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYNPFMRVTELAVQNHTGKNDPIDTMKA 115
P + K+ S + + + PST G ++L+ ++ ++ +HT DP+D
Sbjct: 2 PPRKKPKSSALKSNKQSSANHSSQPSTFGIQQLFLRHIQNSQSTSNSHTSTADPVDQQNV 61
Query: 114 IRLEKDT 94
L DT
Sbjct: 62 NGLASDT 68
>At4g19900.1 68417.m02916 glycosyl transferase-related contains Pfam
profiles PF01535: PPR repeat, PF04572: Alpha
1,4-glycosyltransferase conserved region, PF04488:
Glycosyltransferase sugar-binding region containing DXD
motif; several hypothetical proteins - Arabidopsis
thaliana
Length = 1302
Score = 29.1 bits (62), Expect = 1.9
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = -3
Query: 507 YFVTAPEEGNDSVVFTGDTLFLGGCGRFFEGTADQMYKALITILSSLPDHTKVFCGHEYT 328
YF G FT +L G C + A ++Y+A+I S P+ T+V +EY
Sbjct: 1156 YFHNMKRHGCVPDSFTYGSLISGLCKKSMVDEACKLYEAMIDRGLSPPEVTRVTLAYEYC 1215
Query: 327 LQN 319
+N
Sbjct: 1216 KRN 1218
>At5g59100.1 68418.m07404 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]
Length = 741
Score = 28.3 bits (60), Expect = 3.3
Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 2/74 (2%)
Frame = -3
Query: 363 DHTKVFCGHEYTLQNLKFAAHVEPTNENVKAKISWSQDRRNQGKPTVPSTIGEEKLYN-P 187
DH CG YT +L+ + N +IS + RN PT+ + + K +N
Sbjct: 601 DHINFLCGLNYTSDHLRI---ISGDNSTCTKEISKTLP-RNLNYPTMSAKVSGTKPFNIT 656
Query: 186 FMR-VTELAVQNHT 148
F R VT + +Q T
Sbjct: 657 FQRTVTNVGMQKST 670
>At2g48050.1 68415.m06014 expressed protein ; expression supported by
MPSS
Length = 1500
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +1
Query: 199 FLFTYRRRHCGLSLVSPVL*PANFGFDILISRFYVSS 309
+L+ +HCGLS+ +P+L F + S F VSS
Sbjct: 1053 YLYQIIIQHCGLSIDAPLLHELGFPTQRIKSSFVVSS 1089
>At1g17370.1 68414.m02118 oligouridylate-binding protein, putative
similar to oligouridylate binding protein [Nicotiana
plumbaginifolia] GI:6996560; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 419
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/50 (28%), Positives = 26/50 (52%)
Frame = -3
Query: 222 PSTIGEEKLYNPFMRVTELAVQNHTGKNDPIDTMKAIRLEKDTFK*ISFY 73
PST + N ++VTE +Q P+++ K IR EK ++ + ++
Sbjct: 50 PSTCRSVYVGNIHIQVTEPLLQEVFAGTGPVESCKLIRKEKSSYGFVHYF 99
>At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1939
Score = 27.5 bits (58), Expect = 5.8
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = -3
Query: 408 DQMYKALITILSSLPDHTKVFCGH 337
+Q + T+L SLP T ++CGH
Sbjct: 41 EQWFTDAFTLLISLPKDTHIWCGH 64
>At5g10650.1 68418.m01233 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 525
Score = 27.1 bits (57), Expect = 7.7
Identities = 21/82 (25%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Frame = -3
Query: 417 GTADQMYKALITILSSLPDHTKVFCGHEYTLQNLKF--AAHVEPTNENVKAKISWSQDRR 244
GT D + ++ S +HT + L+NL + V PTN N KIS ++ +
Sbjct: 175 GTRDALTGPSVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNSATKISVTKKKN 234
Query: 243 NQGKPTVPSTIGEEKLYNPFMR 178
G+ ++ S + + P +R
Sbjct: 235 ADGESSLSSKGSKTSVLVPKVR 256
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,385,151
Number of Sequences: 28952
Number of extensions: 225806
Number of successful extensions: 507
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 498
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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