SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS302D01f
         (521 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g35680.2 68418.m04264 eukaryotic translation initiation facto...   163   6e-41
At5g35680.1 68418.m04263 eukaryotic translation initiation facto...   163   6e-41
At2g04520.1 68415.m00458 eukaryotic translation initiation facto...   162   1e-40
At2g40780.1 68415.m05031 hypothetical protein                          35   0.029
At1g17600.1 68414.m02173 disease resistance protein (TIR-NBS-LRR...    31   0.62 
At3g27785.1 68416.m03466 myb family transcription factor (MYB118...    29   1.4  
At4g38270.1 68417.m05406 glycosyl transferase family 8 protein c...    28   4.4  
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam...    28   4.4  
At5g40360.1 68418.m04896 myb family transcription factor (MYB115...    27   5.8  
At2g28560.1 68415.m03470 expressed protein                             27   5.8  
At5g35180.1 68418.m04169 expressed protein                             27   7.7  
At2g07690.1 68415.m00993 minichromosome maintenance family prote...    27   7.7  

>At5g35680.2 68418.m04264 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  163 bits (396), Expect = 6e-41
 Identities = 74/105 (70%), Positives = 85/105 (80%)
 Frame = +2

Query: 140 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 319
           L+FKEDGQEYAQV +MLGNGR + MC DG+KRLCHIRGK+ KKVWI  GDIIL+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKKVWIAAGDIILVGLRDYQ 85

Query: 320 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDEDI 454
           D KADVILKY  DEAR LK YGE PE  R+NE +V  +D  D+D+
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLDD-DDDV 129


>At5g35680.1 68418.m04263 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  163 bits (396), Expect = 6e-41
 Identities = 74/105 (70%), Positives = 85/105 (80%)
 Frame = +2

Query: 140 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 319
           L+FKEDGQEYAQV +MLGNGR + MC DG+KRLCHIRGK+ KKVWI  GDIIL+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKKVWIAAGDIILVGLRDYQ 85

Query: 320 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDEDI 454
           D KADVILKY  DEAR LK YGE PE  R+NE +V  +D  D+D+
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLDD-DDDV 129


>At2g04520.1 68415.m00458 eukaryotic translation initiation factor
           1A, putative / eIF-1A, putative / eIF-4C, putative
           strong similarity to translation initiation factor
           (eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
           profile PF01176: Eukaryotic initiation factor 1A
          Length = 145

 Score =  162 bits (393), Expect = 1e-40
 Identities = 73/104 (70%), Positives = 84/104 (80%)
 Frame = +2

Query: 140 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 319
           L+FKEDGQEYAQV +MLGNGR EAMC DG KRLCHIRGK+ KKVWI  GDI+L+GLRDYQ
Sbjct: 26  LIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIVLVGLRDYQ 85

Query: 320 DAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDED 451
           D KADVILKY  DEAR LK YGE PE  R+NE +V  ++  D++
Sbjct: 86  DDKADVILKYMSDEARLLKAYGELPENTRLNEGIVGDLEDDDDN 129


>At2g40780.1 68415.m05031 hypothetical protein 
          Length = 171

 Score = 35.1 bits (77), Expect = 0.029
 Identities = 16/50 (32%), Positives = 26/50 (52%)
 Frame = +2

Query: 152 EDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILI 301
           E+ Q  AQV  + G+ ++E M   G   L     K R+ +WI +G  ++I
Sbjct: 20  EECQSIAQVVSLRGSNQIEIMDAKGENSLALFPAKFRESMWIRRGSFVVI 69


>At1g17600.1 68414.m02173 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 1025

 Score = 30.7 bits (66), Expect = 0.62
 Identities = 13/47 (27%), Positives = 25/47 (53%)
 Frame = +2

Query: 173 QVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRD 313
           ++ K LGN ++  +  DG+ ++  +    ++K W   G  I+I  RD
Sbjct: 280 EIKKRLGNQKV-FLVLDGVDKVAQVHALAKEKNWFGPGSRIIITTRD 325


>At3g27785.1 68416.m03466 myb family transcription factor (MYB118)
           contains PFAM profile: PF00249 myb-like DNA binding
           domain
          Length = 437

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 16/47 (34%), Positives = 27/47 (57%)
 Frame = +2

Query: 161 QEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILI 301
           ++++Q+ KML  GR+   C +      H+R  ++K  W  + DIILI
Sbjct: 210 KKWSQIAKML-QGRVGKQCRERWHN--HLRPDIKKDGWTEEEDIILI 253


>At4g38270.1 68417.m05406 glycosyl transferase family 8 protein
           contains Pfam profile: PF01501 glycosyl transferase
           family 8
          Length = 680

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 13/48 (27%), Positives = 21/48 (43%)
 Frame = +2

Query: 308 RDYQDAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDED 451
           RDY+   AD ILK   D+    K Y    ++  +    V+ +    E+
Sbjct: 207 RDYESPNADAILKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGEN 254


>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
           protein similar to  PHO1 protein [Arabidopsis thaliana]
           GI:20069032; contains Pfam profiles PF03105: SPX domain,
           PF03124: EXS family
          Length = 756

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = -2

Query: 148 KDQLTFFSFIFVLSSPPIFSSFTFVFRHVL 59
           KD   F +F+F++S+ P FS F    R +L
Sbjct: 562 KDSQVFNTFLFIVSAFPFFSRFLQCMRRML 591


>At5g40360.1 68418.m04896 myb family transcription factor (MYB115)
           contains Pfam profile: PF00249 myb-like DNA-binding
           domain
          Length = 359

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 19/78 (24%), Positives = 33/78 (42%)
 Frame = +2

Query: 140 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRLCHIRGKLRKKVWINQGDIILIGLRDYQ 319
           +V  +  + +  + KM   GR+   C +      H+R  ++K  W  + D ILI +    
Sbjct: 172 MVKSKGTKNWTSIAKMF-QGRVGKQCRERWHN--HLRPNIKKNDWSEEEDQILIEVHKIV 228

Query: 320 DAKADVILKYTPDEARNL 373
             K   I K  P  + N+
Sbjct: 229 GNKWTEIAKRLPGRSENI 246


>At2g28560.1 68415.m03470 expressed protein 
          Length = 320

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
 Frame = -2

Query: 178 DLGVLLSVFLKDQLTFFSFIFVLSSPPIFS-SFTFVFRHVLYDFYYKHKQLHYKSL 14
           +L  LL V +K+  +  SFI   +SPP  S S  F F+ V  +    H   H K L
Sbjct: 5   ELMELLDVGMKEIRSAISFISEATSPPCQSVSSLFFFKKVENEHLSGHLPTHLKGL 60


>At5g35180.1 68418.m04169 expressed protein
          Length = 778

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +3

Query: 117 KMKLKNVSWSLRKTDKSTPKSQRCS 191
           K+KLKNVSW++       PK+   S
Sbjct: 506 KVKLKNVSWAIASLSLKRPKAPGAS 530


>At2g07690.1 68415.m00993 minichromosome maintenance family protein
           / MCM family protein similar to SP|P55862 DNA
           replication licensing factor MCM5 (CDC46 homolog)
           {Xenopus laevis}; contains Pfam profile PF00493:
           MCM2/3/5 family
          Length = 727

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
 Frame = +2

Query: 269 VWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVR-INETVVYSVDGLD 445
           V I Q  I ++GL D  +A +     +TPDE    K + +  +  + I   +  S+ G  
Sbjct: 282 VAIRQPYIRVVGLEDTNEASSRGPANFTPDEEEEFKKFADSQDVYKNICTKIAPSIFG-H 340

Query: 446 EDIE 457
           ED++
Sbjct: 341 EDVK 344


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,324,054
Number of Sequences: 28952
Number of extensions: 169410
Number of successful extensions: 535
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 530
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 535
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -