BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS302C12f (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 185 3e-48 SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces... 30 0.24 SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 28 0.73 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 28 0.97 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 1.3 SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces po... 27 2.2 SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 27 2.2 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 27 2.2 SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyce... 26 3.0 SPBC21B10.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 26 3.0 SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyce... 26 3.9 SPAPYUG7.05 |||delta-1-pyrroline-5-carboxylate reductase |Schizo... 26 3.9 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 25 5.2 SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo... 25 6.8 SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 6.8 SPBC317.01 |mbx2|pvg4|MADS-box transcription factor Pvg4|Schizos... 25 9.0 SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 25 9.0 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 25 9.0 SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3 |Schizosac... 25 9.0 SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch... 25 9.0 >SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1078 Score = 185 bits (451), Expect = 3e-48 Identities = 86/170 (50%), Positives = 121/170 (71%) Frame = +1 Query: 4 GVAVTKQPLIKNMRIIKKETLNLISSWVTRSIDNSMVLENFIPPLLDAVLLDYQRTAVPE 183 G TK P ++ +R IKKE L L+ ++++R+ D +V IP L +AVLLDY + VP+ Sbjct: 737 GNIATKTPHVRGLRTIKKEILKLVDAYISRAEDLELVGNTLIPALFEAVLLDYLQN-VPD 795 Query: 184 AREPEVLSCMAAIVHRLEGHITSEVPKIFDAVFECTLEMINKDFEEYPEHRTEFFLLLQA 363 AR+ EVL+ + IV++L +T ++P + DAVF CTLEMI+KDF EYPEHR FF LL+A Sbjct: 796 ARDAEVLNLITTIVNQLSELLTDKIPLVLDAVFGCTLEMISKDFSEYPEHRAAFFQLLRA 855 Query: 364 VNTNCFKAFLSIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILYRLLLNV 513 +N NCF A L+IP QFKLV++SI+W+FKH R++ +TGL IL L+ N+ Sbjct: 856 INLNCFPALLNIPAPQFKLVINSIVWSFKHVSRDIQETGLNILLELINNM 905 >SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 29.9 bits (64), Expect = 0.24 Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 12/108 (11%) Frame = +1 Query: 190 EPEVLSCMAAIVHRLEGH-ITSEVPKIFDAVFECTLEMINKDFEEYPEHRTE-------- 342 E ++SC+ ++HR+ G IT K+FD E + F+ + +H T+ Sbjct: 98 EASLISCLRWLLHRIPGGVITWSTYKLFDEA-ETRANYPVRGFDIFMKHATKHSCHFNIL 156 Query: 343 --FFLLLQAVNTNCFKAFLSIPPAQFKLVLD-SIIWAFKHTMRNVADT 477 F LL +++ + S + +LV + IWAF M N+ +T Sbjct: 157 KCFLKLLMSLSAKLAVSSNSSTVSSLELVSQIASIWAFDWPMMNLQET 204 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 28.3 bits (60), Expect = 0.73 Identities = 16/46 (34%), Positives = 27/46 (58%) Frame = -1 Query: 389 NALKQFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKTASNIFGTS 252 N +++ VL A ++K ++ S K L ISR+++K S +FG S Sbjct: 994 NTIEERVL-ALQDRKRELIDSALGEKGLREISRLNTKELSFLFGMS 1038 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 27.9 bits (59), Expect = 0.97 Identities = 19/58 (32%), Positives = 27/58 (46%), Gaps = 1/58 (1%) Frame = -1 Query: 506 NNSRYNICK-PVSATFLIVCLNAQIMESKTNLNCAGGMLKNALKQFVLTACSNKKNSV 336 N SRY + K P+ L++ + K+N N G L L Q LT S +K+ V Sbjct: 733 NASRYVVSKRPIPLDLLVLSPKMDDFQLKSNTNKFLGSLAGNLPQESLTTKSKRKSKV 790 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 27.5 bits (58), Expect = 1.3 Identities = 21/60 (35%), Positives = 29/60 (48%) Frame = -1 Query: 320 SSKSLLIISRVHSKTASNIFGTSEVICPSSLCTIAAMQDSTSGSRASGTAVLW*SKSTAS 141 +S S L S V S ++S +S + SSL T + S+S S S + S STAS Sbjct: 205 TSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTAS 264 Score = 24.6 bits (51), Expect = 9.0 Identities = 28/113 (24%), Positives = 50/113 (44%) Frame = -1 Query: 518 SSTFNNSRYNICKPVSATFLIVCLNAQIMESKTNLNCAGGMLKNALKQFVLTACSNKKNS 339 SS+ ++S P S++ I ++ S + + ++ V ++ S+ +S Sbjct: 323 SSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSSTTSSSKSSSSFSSTV-SSSSSTSSS 381 Query: 338 VLCSGYSSKSLLIISRVHSKTASNIFGTSEVICPSSLCTIAAMQDSTSGSRAS 180 L S SS S S HS + S+ +S S+ + A +STS SR+S Sbjct: 382 TLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHNSTS-SRSS 433 >SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 562 Score = 26.6 bits (56), Expect = 2.2 Identities = 15/48 (31%), Positives = 22/48 (45%) Frame = +2 Query: 71 LFQVGLHARLITVWFWKTLFHLFSMLCFWITKGLLFLKLVSLKYCLAW 214 +F+V LH I V+ F + +C GLL L L+ + L W Sbjct: 28 IFEVFLHFLKIPVYMTHLGFPITPTVCLIEMLGLLLLLLIPALFTLIW 75 >SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 752 Score = 26.6 bits (56), Expect = 2.2 Identities = 17/65 (26%), Positives = 27/65 (41%), Gaps = 1/65 (1%) Frame = -3 Query: 387 CFETVCIDRLQ**EKFCSMFWIL-FKVFVNHFQSTFKDCIKYFWYFRSYMSFKSVHDCCH 211 C+ET + E+ +F IL V N +F D + F M +V++C Sbjct: 61 CYETARKSEIGLEERLKCLFAILDLLVIGNEINESFCDHLLPFLILEELMDIHTVNECAK 120 Query: 210 ARQYF 196 +YF Sbjct: 121 LYEYF 125 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 26.6 bits (56), Expect = 2.2 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 3/64 (4%) Frame = +1 Query: 196 EVLSCMAAIVHRLEGHITSEVPKIFDAVFECTLEMI--NKDFEEYPEHRTEFFL-LLQAV 366 ++L CM+ V R+EG TS KIF + + E+I + F+ Y L + + V Sbjct: 1184 DILCCMSDFVSRVEGENTS---KIFTEISKFVREIISSSNSFKNYLLVCLRMLLEVAKNV 1240 Query: 367 NTNC 378 NT C Sbjct: 1241 NTYC 1244 >SPAC5H10.12c |||acetylglucosaminyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 371 Score = 26.2 bits (55), Expect = 3.0 Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +1 Query: 289 TLEMINKDFEEYPEHRTEFF-LLLQAVNTNCFKAFLSIPP 405 T+ N+DF+EY R+EF+ LL AV+ SIPP Sbjct: 219 TVYHFNEDFKEYGASRSEFYPYLLAAVSDR--GEHHSIPP 256 >SPBC21B10.06c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 26.2 bits (55), Expect = 3.0 Identities = 14/45 (31%), Positives = 21/45 (46%), Gaps = 1/45 (2%) Frame = -3 Query: 249 SYMSFKSVHDCCHARQYF-RLTSFRNSSPLVIQKHSIEKRWNKVF 118 SY+ F S+ CH YF R+ + + V H E N+V+ Sbjct: 147 SYICFSSLIRICHCWTYFIRIMGLSSLNKFVNLLHDFETTSNRVY 191 >SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 25.8 bits (54), Expect = 3.9 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +3 Query: 432 HNLGI*TYNEECGRYRFTNIVPTVVKC*G 518 H +GI T E C TN +PTV++ G Sbjct: 505 HGIGIFTAKEICENNTITNELPTVLRSVG 533 >SPAPYUG7.05 |||delta-1-pyrroline-5-carboxylate reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 282 Score = 25.8 bits (54), Expect = 3.9 Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 1/59 (1%) Frame = -1 Query: 395 LKNALK-QFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKTASNIFGTSEVICPSSLCT 222 +K ALK + +L+ + K S L S + +I R+ TAS I + VICP T Sbjct: 99 MKEALKGKLILSILAGKTISSLQSMLDESTRVI--RIMPNTASRIRESMSVICPGPNAT 155 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 25.4 bits (53), Expect = 5.2 Identities = 15/49 (30%), Positives = 29/49 (59%) Frame = +1 Query: 133 PLLDAVLLDYQRTAVPEAREPEVLSCMAAIVHRLEGHITSEVPKIFDAV 279 PLLD V+ TAVPE+ +PE +S + + +E + S++ ++ + + Sbjct: 965 PLLDQVIA----TAVPESSDPEAVSIVVKAL--MEVDLPSQLIELLEKI 1007 >SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 840 Score = 25.0 bits (52), Expect = 6.8 Identities = 11/72 (15%), Positives = 37/72 (51%), Gaps = 1/72 (1%) Frame = +2 Query: 179 LKLVSLKYCLAWQQSCTDLKDI*LLKYQKYLMQSLNVLWK*LTKTLKSIQNIEQNFS-YY 355 ++ ++ +Y + W+ S D+K++ + + + + +W + ++QN ++ Sbjct: 532 IQKIAKQYLIKWRISLLDIKELSIRAEKLHTTNEIIGVWNQWVVSYNRKVGMQQNADLFF 591 Query: 356 CRRSIQTVSKHF 391 R++++ V +H+ Sbjct: 592 KRKTLRVVFEHW 603 >SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 25.0 bits (52), Expect = 6.8 Identities = 13/50 (26%), Positives = 22/50 (44%) Frame = -3 Query: 339 CSMFWILFKVFVNHFQSTFKDCIKYFWYFRSYMSFKSVHDCCHARQYFRL 190 C F I +F++ + S +F+YF + F + H +FRL Sbjct: 88 CRRFHIA--IFIHPYDSNVVPFFCFFFYFSLFSFFSFLFTSLHFNFFFRL 135 >SPBC317.01 |mbx2|pvg4|MADS-box transcription factor Pvg4|Schizosaccharomyces pombe|chr 2|||Manual Length = 372 Score = 24.6 bits (51), Expect = 9.0 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = -2 Query: 124 SFPKPYCYQSSV*P 83 ++P PYC+QS V P Sbjct: 188 NYPPPYCFQSPVSP 201 >SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces pombe|chr 1|||Manual Length = 4924 Score = 24.6 bits (51), Expect = 9.0 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -2 Query: 262 LVLQKLYVLQVCARLLPCKTVLQAHELQEQQS 167 L+ KL +VC +L K LQ H++ QS Sbjct: 1600 LLDSKLSFAEVCGQLQRLKETLQEHQMTSVQS 1631 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 24.6 bits (51), Expect = 9.0 Identities = 8/26 (30%), Positives = 17/26 (65%) Frame = +1 Query: 244 ITSEVPKIFDAVFECTLEMINKDFEE 321 + V IF+AV +CT + + +++E+ Sbjct: 730 VPDTVKNIFEAVRKCTFDTLEEEYEK 755 >SPCC645.04 |nse3||Smc5-6 complex non-SMC subunit Nse3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 328 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/29 (34%), Positives = 15/29 (51%) Frame = +3 Query: 360 GGQYKLFQSIFEHTTSTIQVGFRFHNLGI 446 G LFQS+FE +Q+ F F + + Sbjct: 124 GTSRNLFQSVFEEADRQLQLSFGFRLVAV 152 >SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 619 Score = 24.6 bits (51), Expect = 9.0 Identities = 12/45 (26%), Positives = 23/45 (51%) Frame = +1 Query: 151 LLDYQRTAVPEAREPEVLSCMAAIVHRLEGHITSEVPKIFDAVFE 285 L+D+ + + E RE + A + L+ H ++PK+ + FE Sbjct: 407 LMDFCCSILIELRESILERNFADSIKLLQAHFNVDMPKLLNLTFE 451 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,347,224 Number of Sequences: 5004 Number of extensions: 50868 Number of successful extensions: 172 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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