Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302C12f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Ex... 181 3e-46
At3g03110.1 68416.m00307 exportin 1, putative strong similarity ... 177 4e-45
At3g45720.1 68416.m04941 proton-dependent oligopeptide transport... 33 0.088
At5g01400.1 68418.m00053 expressed protein contains low similari... 32 0.27
At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s... 31 0.36
At4g39440.1 68417.m05581 expressed protein ; expression support... 31 0.62
At5g61670.2 68418.m07738 expressed protein 28 3.3
At5g61670.1 68418.m07737 expressed protein 28 3.3
At3g03960.1 68416.m00415 chaperonin, putative similar to SWISS-P... 28 3.3
At1g75430.1 68414.m08762 homeodomain-containing protein contains... 28 4.4
At5g50140.1 68418.m06210 ankyrin repeat family protein contains ... 27 5.8
At3g59950.2 68416.m06690 autophagy 4b (APG4b) identical to autop... 27 7.7
At3g59950.1 68416.m06691 autophagy 4b (APG4b) identical to autop... 27 7.7
At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase fa... 27 7.7
At1g25290.1 68414.m03139 rhomboid family protein contains PFAM d... 27 7.7
At1g16070.1 68414.m01928 tubby family protein similar to Tubby r... 27 7.7
>At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to
Exportin1 (XPO1) protein [Arabidopsis thaliana]
GI:7671510
Length = 1075
Score = 181 bits (440), Expect = 3e-46
Identities = 82/172 (47%), Positives = 123/172 (71%)
Frame = +1
Query: 4 GVAVTKQPLIKNMRIIKKETLNLISSWVTRSIDNSMVLENFIPPLLDAVLLDYQRTAVPE 183
G +K +K +R +K+ETL LI +++ ++ D + + F+PP++++VL DY R VP+
Sbjct: 739 GPYASKTSFVKLLRSVKRETLKLIETFLDKAEDQPHIGKQFVPPMMESVLGDYARN-VPD 797
Query: 184 AREPEVLSCMAAIVHRLEGHITSEVPKIFDAVFECTLEMINKDFEEYPEHRTEFFLLLQA 363
ARE EVLS A I+++ + + +VP IF+AVF+CTLEMI K+FE+YPEHR +FF LL+A
Sbjct: 798 ARESEVLSLFATIINKYKATMLDDVPHIFEAVFQCTLEMITKNFEDYPEHRLKFFSLLRA 857
Query: 364 VNTNCFKAFLSIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILYRLLLNVEE 519
+ T CF A + + Q KLV+DSIIWAF+HT RN+A+TGL +L +L N ++
Sbjct: 858 IATFCFPALIKLSSPQLKLVMDSIIWAFRHTERNIAETGLNLLLEMLKNFQQ 909
>At3g03110.1 68416.m00307 exportin 1, putative strong similarity to
Exportin1 (XPO1) protein [Arabidopsis thaliana]
GI:7671510; contains Pfam profile PF03810: Importin-beta
N-terminal domain
Length = 1076
Score = 177 bits (431), Expect = 4e-45
Identities = 81/172 (47%), Positives = 123/172 (71%)
Frame = +1
Query: 4 GVAVTKQPLIKNMRIIKKETLNLISSWVTRSIDNSMVLENFIPPLLDAVLLDYQRTAVPE 183
G ++ L+K +R +K+E L LI +++ ++ + + + F+PP++D VL DY R VP+
Sbjct: 740 GPYASRTSLVKLLRSVKREILKLIETFLDKAENQPHIGKQFVPPMMDQVLGDYARN-VPD 798
Query: 184 AREPEVLSCMAAIVHRLEGHITSEVPKIFDAVFECTLEMINKDFEEYPEHRTEFFLLLQA 363
ARE EVLS A I+++ + + EVP IF+AVF+CTLEMI K+FE+YPEHR +FF LL+A
Sbjct: 799 ARESEVLSLFATIINKYKVVMRDEVPLIFEAVFQCTLEMITKNFEDYPEHRLKFFSLLRA 858
Query: 364 VNTNCFKAFLSIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILYRLLLNVEE 519
+ T CF+A + + Q KLV+DS+IWAF+HT RN+A+TGL +L +L N ++
Sbjct: 859 IATFCFRALIQLSSEQLKLVMDSVIWAFRHTERNIAETGLNLLLEMLKNFQK 910
>At3g45720.1 68416.m04941 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 555
Score = 33.5 bits (73), Expect = 0.088
Identities = 29/114 (25%), Positives = 54/114 (47%), Gaps = 3/114 (2%)
Frame = -1
Query: 350 KKNSVLCSGYSSKSLLIISRVHSKTASNIFGTSEVIC--PSSLCTIAAMQDSTSGSRASG 177
+K S C G SKS ++R K+ ++ + +C P LC++ ++D S R
Sbjct: 268 EKESKTCIGMPSKSFRFLNRAALKSEKDL-NQEDGLCHNPWRLCSVEEVEDFKSVLR--- 323
Query: 176 TAVLW*SKSTASRRGGIKFSKTILLSIERVTQLEIRFKV-SFFIILIFLMRGCL 18
LW + G++ S T+L ++ L+ +FKV + + +I L+ C+
Sbjct: 324 VLPLWLAILFVGTSIGVQASMTVLQALVTDRGLDSKFKVPAGSLQVIVLISSCV 377
>At5g01400.1 68418.m00053 expressed protein contains low similarity to
symplekin SP:Q92797 from [Homo sapiens]
Length = 1467
Score = 31.9 bits (69), Expect = 0.27
Identities = 15/59 (25%), Positives = 24/59 (40%)
Frame = +1
Query: 259 PKIFDAVFECTLEMINKDFEEYPEHRTEFFLLLQAVNTNCFKAFLSIPPAQFKLVLDSI 435
P + D + E +++K +YP+ F Q +K L +PP Q L I
Sbjct: 1154 PALSDFILEILSRLVSKQIWKYPKLWVGFLKCTQTTQPQSYKVLLQLPPLQLGNALTKI 1212
>At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative
similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha
[Drosophila melanogaster]
Length = 926
Score = 31.5 bits (68), Expect = 0.36
Identities = 25/108 (23%), Positives = 45/108 (41%)
Frame = -1
Query: 455 VCLNAQIMESKTNLNCAGGMLKNALKQFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKT 276
VC++ Q + NC + + + T N +++V C+ S+ +L + +
Sbjct: 766 VCIHCQ-QRGHASTNCPSRVPASRNSRPTATNPRNDESTVSCNTCGSQCVLRTANTEANR 824
Query: 275 ASNIFGTSEVICPSSLCTIAAMQDSTSGSRASGTAVLW*SKSTASRRG 132
F CP+ C+ A +DS + S SG A + + RRG
Sbjct: 825 GRQFFS-----CPTQGCSFFAWEDSINNS--SGNATTGSNSGGSGRRG 865
>At4g39440.1 68417.m05581 expressed protein ; expression supported
by MPSS
Length = 443
Score = 30.7 bits (66), Expect = 0.62
Identities = 17/48 (35%), Positives = 29/48 (60%)
Frame = -2
Query: 319 LQSLC*SFPEYIQRLHQIFLVLQKLYVLQVCARLLPCKTVLQAHELQE 176
LQ L FP+ ++RL Q+++ + + VL V LL C T+LQ +++
Sbjct: 376 LQMLPCWFPKAVRRLVQLYIQV-SISVLSVVCALLSCTTLLQLSGIED 422
>At5g61670.2 68418.m07738 expressed protein
Length = 307
Score = 28.3 bits (60), Expect = 3.3
Identities = 25/90 (27%), Positives = 37/90 (41%), Gaps = 1/90 (1%)
Frame = -1
Query: 476 VSATFLIVCLNAQIMESKTNLNCAG-GMLKNALKQFVLTACSNKKNSVLCSGYSSKSLLI 300
+SA ++ N + E K C G G L A + S + G S
Sbjct: 211 ISALMVVEVNNVKQQEHKRCKYCLGTGYLACARCSSTGALVLTEPVSAIAGGNHS----- 265
Query: 299 ISRVHSKTASNIFGTSEVICPSSLCTIAAM 210
+S ++ SN G +V+CP+ LCT AM
Sbjct: 266 LSPPKTERCSNCSGAGKVMCPTCLCTGMAM 295
>At5g61670.1 68418.m07737 expressed protein
Length = 307
Score = 28.3 bits (60), Expect = 3.3
Identities = 25/90 (27%), Positives = 37/90 (41%), Gaps = 1/90 (1%)
Frame = -1
Query: 476 VSATFLIVCLNAQIMESKTNLNCAG-GMLKNALKQFVLTACSNKKNSVLCSGYSSKSLLI 300
+SA ++ N + E K C G G L A + S + G S
Sbjct: 211 ISALMVVEVNNVKQQEHKRCKYCLGTGYLACARCSSTGALVLTEPVSAIAGGNHS----- 265
Query: 299 ISRVHSKTASNIFGTSEVICPSSLCTIAAM 210
+S ++ SN G +V+CP+ LCT AM
Sbjct: 266 LSPPKTERCSNCSGAGKVMCPTCLCTGMAM 295
>At3g03960.1 68416.m00415 chaperonin, putative similar to
SWISS-PROT:P42932- T-complex protein 1, theta subunit
(TCP-1-theta) [Mus musculus]; contains Pfam:PF00118
domain, TCP-1/cpn60 chaperonin family
Length = 549
Score = 28.3 bits (60), Expect = 3.3
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = -1
Query: 302 IISRVHSKTASNIFGTSEVICPSSLCTIAAMQ 207
+ISR+ + AS FG E+IC SL T A +Q
Sbjct: 162 VISRMRAAVASKQFGQEEIIC--SLVTDACIQ 191
>At1g75430.1 68414.m08762 homeodomain-containing protein contains
'Homeobox' domain signature, Prosite:PS00027
Length = 297
Score = 27.9 bits (59), Expect = 4.4
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 302 LTKTLKSIQNIEQNFSYYCRRSIQTVS 382
+TK L +Q +E+ F YC + Q +S
Sbjct: 89 ITKLLSLLQQVEERFEQYCNQLEQVIS 115
>At5g50140.1 68418.m06210 ankyrin repeat family protein contains
ankyrin repeat domains, Pfam:PF00023
Length = 535
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +1
Query: 169 TAVPEAREPEVLSCMAAIVHRLEGHITSEVPKIFDAVFECTLEMINKDFEEY 324
T + A LS ++ IVH ++ +T++ K F+AV +++NKD E++
Sbjct: 182 TVLHAAASVGFLSLVSYIVHEIKIEVTTQNDKGFEAV-----DLLNKDDEDF 228
>At3g59950.2 68416.m06690 autophagy 4b (APG4b) identical to
autophagy 4b [Arabidopsis thaliana]
gi|19912145|dbj|BAB88384
Length = 471
Score = 27.1 bits (57), Expect = 7.7
Identities = 20/68 (29%), Positives = 31/68 (45%)
Frame = -1
Query: 395 LKNALKQFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKTASNIFGTSEVICPSSLCTIA 216
+K +FV + CS+ S SS + L+ S SN+ S+V+ SS +
Sbjct: 1 MKAICDRFVPSKCSSSSTSEK-RDISSPTSLVSDSASSDNKSNLTLCSDVVASSSPVSQL 59
Query: 215 AMQDSTSG 192
+ STSG
Sbjct: 60 CREASTSG 67
>At3g59950.1 68416.m06691 autophagy 4b (APG4b) identical to
autophagy 4b [Arabidopsis thaliana]
gi|19912145|dbj|BAB88384
Length = 477
Score = 27.1 bits (57), Expect = 7.7
Identities = 20/68 (29%), Positives = 31/68 (45%)
Frame = -1
Query: 395 LKNALKQFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKTASNIFGTSEVICPSSLCTIA 216
+K +FV + CS+ S SS + L+ S SN+ S+V+ SS +
Sbjct: 1 MKAICDRFVPSKCSSSSTSEK-RDISSPTSLVSDSASSDNKSNLTLCSDVVASSSPVSQL 59
Query: 215 AMQDSTSG 192
+ STSG
Sbjct: 60 CREASTSG 67
>At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase
family protein belongs to Pfam:PF03372:
Endonuclease/Exonuclease/phosphatase family; contains 3
WD-40 repeats (PF00400);similar to Type II
inositol-1,4,5-trisphosphate 5-phosphatase EC 3.1.3.56
(5PTASE) (INPP5B) (SP:P32019) [Homo sapiens]
Length = 1305
Score = 27.1 bits (57), Expect = 7.7
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +1
Query: 226 HRLEGHITSEVPKIFDAVFECTLEMINKDFEEYP 327
HR H ++PKI + V C + + D E YP
Sbjct: 48 HRQRQHSLDDIPKITEIVSGCGISGDSSDDEFYP 81
>At1g25290.1 68414.m03139 rhomboid family protein contains PFAM
domain PF01694, Rhomboid family
Length = 343
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -3
Query: 255 FRSYMSFKSVHDCCHARQYFRLTSFRNSSPLVIQKHSIEKR 133
FR + + SVH C H ++ RL SS +Q S + R
Sbjct: 21 FRGFATAASVHACHHVSRHLRLDFHLRSSLKKLQHFSDDAR 61
>At1g16070.1 68414.m01928 tubby family protein similar to Tubby
related protein 1 (Tubby-like protein 1)
(Swiss-Prot:Q9Z273) [Mus musculus]; low similarity to
Tubby related protein 1 (Tubby-like protein 1)
(Swiss-Prot:O0029) {Homo sapiens}; similar to
phosphodiesterase (GI:467578) [Mus musculus]; contains
Pfam profile PF01167: Tub family
Length = 397
Score = 27.1 bits (57), Expect = 7.7
Identities = 19/59 (32%), Positives = 26/59 (44%), Gaps = 1/59 (1%)
Frame = -1
Query: 392 KNALKQFVLTACSNKKNSVLCSGYSSKSLLIISRVHSKTASN-IFGTSEVICPSSLCTI 219
+ ALKQ L AC K + V S + K+ + HS + F SE SS T+
Sbjct: 80 RTALKQSSLQACMQKNSEVDKSSFGMKTWTSVDSEHSSSLKVWEFSDSEAAPASSWSTL 138
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,878,712
Number of Sequences: 28952
Number of extensions: 250620
Number of successful extensions: 726
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 724
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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