BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302B08f
(521 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane synthase|Schiz... 29 0.56
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 28 0.97
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 27 1.3
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 27 2.2
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces... 26 3.0
SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy... 26 3.9
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 26 3.9
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 25 6.8
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 6.8
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 6.8
SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomy... 25 6.8
SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizo... 25 9.0
SPCC24B10.08c |||histone acetyltransferase complex subunit Ada2 ... 25 9.0
>SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane
synthase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 28.7 bits (61), Expect = 0.56
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +3
Query: 9 GIRSLWEKNDATLTTDKEETERGATVTIALLK 104
G+R+ + +A +++++E E G TV +ALLK
Sbjct: 282 GLRAAFGNAEAVVSSEEEAEELGITVALALLK 313
>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 27.9 bits (59), Expect = 0.97
Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +2
Query: 83 GHNR-SFESKDSLPEWVTYEGVWDMETQDPV-KEFIRAI 193
G+NR SF+SK+ LP+W E + P+ KE + A+
Sbjct: 647 GYNRWSFQSKEGLPDWFLDEETTVNKPNKPITKEAVLAL 685
>SPAC1556.02c |sdh1||succinate dehydrogenase
Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 27.5 bits (58), Expect = 1.3
Identities = 15/44 (34%), Positives = 20/44 (45%), Gaps = 1/44 (2%)
Frame = +2
Query: 65 NRKRSNG-HNRSFESKDSLPEWVTYEGVWDMETQDPVKEFIRAI 193
NRK S G H R + W+ + W +T DPV RA+
Sbjct: 578 NRKESRGAHAREDYPERDDKNWIKHTLTWQHKTGDPVTLKYRAV 621
>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 606
Score = 26.6 bits (56), Expect = 2.2
Identities = 17/62 (27%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 29 KERRNSYHGQRRNRKRSNGHNRSFESKDSLPEWVTYEGVWDMETQDPVKEFIRAIA-LDE 205
KE+RN+ +R+++K ++ S + E V+ G + E D + E A++ L+E
Sbjct: 526 KEKRNTRREKRKSKKEFLKAQKNEASNNLKQEIVSKAGAQETENDDLIDEESDALSELEE 585
Query: 206 GY 211
Y
Sbjct: 586 DY 587
>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 224
Score = 26.2 bits (55), Expect = 3.0
Identities = 18/67 (26%), Positives = 34/67 (50%), Gaps = 9/67 (13%)
Frame = +2
Query: 8 RNKKFVGKERRNSYHGQRRNRK-------RSNGHNRSFESKDSLPEWVTY--EGVWDMET 160
RN++ + KE R+S+H + + + N N+S ESK+S V + + + T
Sbjct: 155 RNREIIEKEERSSFHFRVKPKNLDKVPKLAENEGNKSLESKESNENKVVFKKKKSGKLRT 214
Query: 161 QDPVKEF 181
+ +KE+
Sbjct: 215 KSSLKEY 221
>SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 370
Score = 25.8 bits (54), Expect = 3.9
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = +2
Query: 137 EGVWDMETQDPVKEFIR--AIALDEGYGTCENTITDDIRNN 253
+G+WD ++ V EF+R +A CEN + I +N
Sbjct: 233 DGIWDCKSSQQVVEFVRRGIVARQSLEVICENLMDRCIASN 273
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.8 bits (54), Expect = 3.9
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -2
Query: 478 RKEVVKVDR*VLVFIIWSVLIPESLNAAIKLRFELG 371
++ V+ R +L F W+VL+P L F++G
Sbjct: 86 KQRVIFFTRVLLTFFCWTVLLPLIFKHVWNLNFKIG 121
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.0 bits (52), Expect = 6.8
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2
Query: 259 WFVVADVISDGVFTSAISLIKSY 191
WF++ VIS G SA SL+K Y
Sbjct: 72 WFIMG-VISTGFAWSAYSLVKKY 93
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 25.0 bits (52), Expect = 6.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -1
Query: 479 KKGGRESRSVGPGIHHLECFDPREPQCCDQTSLRTWRKAPH 357
K+ E RS+ P ++ F+P +P C W+KA H
Sbjct: 337 KRLSSELRSLAPANWEVKVFEPSDPICF------PWKKASH 371
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 25.0 bits (52), Expect = 6.8
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = -2
Query: 328 IISKYPISIEVKSIIPIRYSIIKWFVVAD 242
II + ++E+ +IIP+RY K +V D
Sbjct: 1376 IIDEAAQAVELDTIIPLRYGAKKCILVGD 1404
>SPAC2G11.07c |ptc3||protein phosphatase 2C Ptc3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 414
Score = 25.0 bits (52), Expect = 6.8
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 137 EGVWDMETQDPVKEFIR 187
+G+WD +T V EF+R
Sbjct: 230 DGIWDCKTSQQVIEFVR 246
>SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase
Ubp12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 979
Score = 24.6 bits (51), Expect = 9.0
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +3
Query: 15 RSLWEKNDATLTTDKEETERGATVTI 92
RSLW ++ L+ K+++E T+T+
Sbjct: 802 RSLWLESKTILSDKKDDSEDSRTITL 827
>SPCC24B10.08c |||histone acetyltransferase complex subunit Ada2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 437
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/30 (33%), Positives = 15/30 (50%)
Frame = -1
Query: 350 ARVCVAPDHQQVPNQYRGQVHHPNQVQHYQ 261
AR+ PD+Q+ N Y Q+ Q+ Q
Sbjct: 259 ARLLTGPDYQKFVNSYHEQITLKKQISDLQ 288
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,073,108
Number of Sequences: 5004
Number of extensions: 40168
Number of successful extensions: 155
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 212331630
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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