BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS302B08f
(521 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g16700.1 68418.m01955 glycosyl hydrolase family 5 protein / c... 30 0.82
At3g50440.1 68416.m05517 hydrolase, alpha/beta fold family prote... 30 0.82
At3g60580.1 68416.m06777 zinc finger (C2H2 type) family protein ... 29 1.4
At1g36990.1 68414.m04611 expressed protein contains PS00070: Ald... 29 1.4
At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa subu... 29 2.5
At4g19580.1 68417.m02878 DNAJ heat shock N-terminal domain-conta... 28 4.4
At3g06510.1 68416.m00755 glycosyl hydrolase family 1 protein sim... 27 5.8
At1g60420.1 68414.m06802 DC1 domain-containing protein contains ... 27 7.7
At1g48370.1 68414.m05403 oligopeptide transporter OPT family pro... 27 7.7
>At5g16700.1 68418.m01955 glycosyl hydrolase family 5 protein /
cellulase family protein cellulase (EC 3.2.1.4)
precursor - Xanthomonas campestris pv. campestris,
PIR:JH0158
Length = 488
Score = 30.3 bits (65), Expect = 0.82
Identities = 13/61 (21%), Positives = 34/61 (55%)
Frame = -2
Query: 325 ISKYPISIEVKSIIPIRYSIIKWFVVADVISDGVFTSAISLIKSYGTNKLFDWILGLHIP 146
+SK P+ K I+ + ++ ++ D++++ +++ +S+ + KLF+ +LG+
Sbjct: 60 LSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTH 119
Query: 145 N 143
N
Sbjct: 120 N 120
>At3g50440.1 68416.m05517 hydrolase, alpha/beta fold family protein
similar to ethylene-induced esterase [Citrus sinensis]
GI:14279437, polyneuridine aldehyde esterase [Rauvolfia
serpentina] GI:6651393; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 275
Score = 30.3 bits (65), Expect = 0.82
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = -1
Query: 308 QYRGQVHHPNQVQ-HYQVVCCCGCHQ*WCFHK 216
QY+ Q HH Q Q H+ V CH WC+ K
Sbjct: 6 QYQMQTHHMQQQQLHHFVFVHGSCHGAWCWFK 37
>At3g60580.1 68416.m06777 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 288
Score = 29.5 bits (63), Expect = 1.4
Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
Frame = +2
Query: 65 NRKRSNGHNRSFES----KDSLPEWVTYEGVWDMETQDPVKEFIRAIALDEGYGTCENT 229
N K GH RS S ++ P ++YE D+ + DP F ++ L++G E++
Sbjct: 15 NGKALGGHMRSHMSNSHEEEQRPSQLSYETESDVSSSDPKFAFTSSVLLEDGESESESS 73
>At1g36990.1 68414.m04611 expressed protein contains PS00070:
Aldehyde dehydrogenases cysteine active site; similar to
high molecular mass nuclear antigen (GI:2754696) [Gallus
gallus];similar to streptococcal hemagglutinin
(GI:8885520) [Streptococcus gordonii] similar to
proteophosphoglycan (GI:5420389) [Leishmania major]
Length = 581
Score = 29.5 bits (63), Expect = 1.4
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = +2
Query: 35 RRNSYHGQRRNRKRSNGHNRSFESKDSL--PEWVTYEGVWDMETQDPVKEFI 184
RR S +G ++ S NRS KD + V+Y WD++T P++ +
Sbjct: 73 RRGSSNGSAKHAYSSFNFNRSQRDKDRSRDKDRVSYVDPWDLDTSIPLRTIL 124
>At2g39960.1 68415.m04910 microsomal signal peptidase 25 kDa
subunit, putative (SPC25) identical to Probable
microsomal signal peptidase 25 kDa subunit (EC 3.4.-.-)
(SPase 25 kDa subunit) (SPC25) (Swiss-Prot:P58684)
[Arabidopsis thaliana]; contains non-consensus AT-AC
splice sites; contains 1 transmembrane domain;
Length = 192
Score = 28.7 bits (61), Expect = 2.5
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = -2
Query: 334 LQIISKYPISIEVKSIIPIRYSIIKWFVVADVISDGVFTSAI-SLIKSY 191
L I S P SI + + S+ +WF V+ +G+F + +LIK+Y
Sbjct: 135 LTIDSADPKSISAGKSVQLTKSVTQWFTKDGVLVEGLFWKDVEALIKNY 183
>At4g19580.1 68417.m02878 DNAJ heat shock N-terminal
domain-containing protein low similarity to SP|Q9QYI4
DnaJ homolog subfamily B member 12 {Mus musculus};
contains Pfam profile PF00226: DnaJ domain
Length = 301
Score = 27.9 bits (59), Expect = 4.4
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -1
Query: 392 QTSLRTWRKAPHRQARVCVAPDHQQVPNQYR 300
Q +T + H + R CVA DH++ P Q +
Sbjct: 117 QGQTQTQKSQNHTRTRTCVAYDHKRKPKQVK 147
>At3g06510.1 68416.m00755 glycosyl hydrolase family 1 protein
similar to Beta-galactosidase (SP:P22498) [Sulfolobus
solfataricus}; almost identical to beta-glucosidase
GB:AAF23823 GI:6685165 from [Arabidopsis thaliana]
Length = 622
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +2
Query: 113 SLPEWVTYEGVWDME-TQDPVKEFIRAIALDEGYGTCENTIT 235
SLP W G W ME T D +F R I +D Y ++ +T
Sbjct: 224 SLPPWAADYGGWKMEKTVDYFMDFTR-IVVDSMYDLVDSWVT 264
>At1g60420.1 68414.m06802 DC1 domain-containing protein contains
Pfam domain PF03107: DC1 domain
Length = 578
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 231 SLMTSATTNHLIMLYLIGMMDLTSILIGYLLMIWSHAHSRLPMRSFTP 374
SL+ S N++ L G L S L+G ++++ AH P R+FTP
Sbjct: 338 SLLVSGDLNYV--LGKDGAKVLVSDLVGKTILMYFSAHWCPPCRAFTP 383
>At1g48370.1 68414.m05403 oligopeptide transporter OPT family
protein similar to iron-phytosiderophore transporter
protein yellow stripe 1 [Zea mays] GI:10770865; contains
Pfam profile PF03169: OPT oligopeptide transporter
protein
Length = 724
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/60 (23%), Positives = 28/60 (46%)
Frame = -2
Query: 340 AWLQIISKYPISIEVKSIIPIRYSIIKWFVVADVISDGVFTSAISLIKSYGTNKLFDWIL 161
+W + IS +I+P +S ++W+ + + +F ++ +YG L DW L
Sbjct: 425 SWFAVGGYVVISAVSTAILPHMFSQLRWYYIIVIY---IFAPILAFCNAYGAG-LTDWSL 480
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,036,139
Number of Sequences: 28952
Number of extensions: 222357
Number of successful extensions: 795
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 794
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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