BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS301F10f (516 letters) Database: human 237,096 sequences; 76,859,062 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DQ535393-1|ABF83336.1| 127|Homo sapiens circulating B cell anti... 29 7.3 AY781845-1|AAW29147.1| 135|Homo sapiens AF1 non-allergic IgE he... 29 9.7 >DQ535393-1|ABF83336.1| 127|Homo sapiens circulating B cell antibody heavy chain variable region protein. Length = 127 Score = 29.5 bits (63), Expect = 7.3 Identities = 22/63 (34%), Positives = 27/63 (42%) Frame = +3 Query: 327 ISMIYSIINRYINPFLKISK*NRVFCMKDTSKLSISIKINMHLT*PNLTKYQGTCADFYI 506 I IY Y NP LK +RV DTSK S+K+N Y T D YI Sbjct: 52 IGSIYYSGRTYYNPSLK----SRVTISVDTSKNQFSLKLNSVTAADTAVYYCATQRDTYI 107 Query: 507 XLL 515 ++ Sbjct: 108 AVI 110 >AY781845-1|AAW29147.1| 135|Homo sapiens AF1 non-allergic IgE heavy chain IGHV4-31 protein. Length = 135 Score = 29.1 bits (62), Expect = 9.7 Identities = 18/40 (45%), Positives = 21/40 (52%) Frame = +3 Query: 327 ISMIYSIINRYINPFLKISK*NRVFCMKDTSKLSISIKIN 446 I IY N Y NP LK +RV DTSK S+K+N Sbjct: 50 IGYIYDSGNTYYNPSLK----SRVTISVDTSKNQFSLKLN 85 Database: human Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 76,859,062 Number of sequences in database: 237,096 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 59,614,281 Number of Sequences: 237096 Number of extensions: 1047052 Number of successful extensions: 1069 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 1069 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1069 length of database: 76,859,062 effective HSP length: 85 effective length of database: 56,705,902 effective search space used: 4876707572 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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