Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS301E04f
         (412 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...   100   2e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    56   2e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    54   9e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    49   2e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    48   6e-07
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    34   0.010
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha...    28   0.49 
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ...    27   1.5  
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma...    25   3.5  
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr...    25   3.5  
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c...    25   6.1  
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb...    24   8.0  
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc...    24   8.0  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 99.5 bits (237), Expect = 2e-22
 Identities = 47/59 (79%), Positives = 52/59 (88%)
 Frame = +3

Query: 63  LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGEYT 239
           LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDADG  T
Sbjct: 6   LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGT 64



 Score = 60.1 bits (139), Expect = 1e-10
 Identities = 30/71 (42%), Positives = 46/71 (64%)
 Frame = +3

Query: 51  MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 230
           M D   EE++   +EAF +FDKDG+G IT +EL  V+ SLG+  ++ E+ DMI E D DG
Sbjct: 78  MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134

Query: 231 EYT*NHMIYAK 263
           +   N+  +++
Sbjct: 135 DGVINYEEFSR 145



 Score = 31.5 bits (68), Expect = 0.053
 Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
 Frame = +3

Query: 81  AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADG 230
           AE ++  +  D DG+GTI   E  T+M R +     E E+++     D DG
Sbjct: 48  AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDG 98


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 56.0 bits (129), Expect = 2e-09
 Identities = 27/51 (52%), Positives = 36/51 (70%)
 Frame = +3

Query: 66  TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 218
           ++EQ  E KEAF L+D D DG I T  +G+V+RSLG N T+AEL  + NE+
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54



 Score = 43.2 bits (97), Expect = 2e-05
 Identities = 22/60 (36%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
 Frame = +3

Query: 45  STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 221
           S ++++L E E   E+ +AF +FDKD  G I T +    M++LG+  ++ E+Q M+ E D
Sbjct: 65  SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 54.0 bits (124), Expect = 9e-09
 Identities = 24/54 (44%), Positives = 35/54 (64%)
 Frame = +3

Query: 60  QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 221
           QLT  QI E KEAF+L DKDGDG I  +++ T++ SL Q+ +E  +  M   ++
Sbjct: 41  QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94



 Score = 26.2 bits (55), Expect = 2.0
 Identities = 12/48 (25%), Positives = 21/48 (43%)
 Frame = +3

Query: 93  EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGEY 236
           EAFS FD    G I    +   + S+G      E++ ++    + G +
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHGVF 168


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 49.2 bits (112), Expect = 2e-07
 Identities = 23/58 (39%), Positives = 34/58 (58%)
 Frame = +3

Query: 60  QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGE 233
           ++TEEQ  +  EAF LFD D D  I   EL   MR+LG N  ++E+  ++ + D  G+
Sbjct: 30  EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGK 87


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 48.0 bits (109), Expect = 6e-07
 Identities = 20/47 (42%), Positives = 33/47 (70%)
 Frame = +3

Query: 87  FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 227
           +K+AFSLFD+ G G I    +G ++R+ GQNPT AE+ ++ + + A+
Sbjct: 8   YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAE 54



 Score = 37.1 bits (82), Expect = 0.001
 Identities = 17/45 (37%), Positives = 26/45 (57%)
 Frame = +3

Query: 84  EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 218
           EF + F +FDKD  G I   EL  V+ SLG+  +  E+ +++  V
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 33.9 bits (74), Expect = 0.010
 Identities = 19/54 (35%), Positives = 29/54 (53%)
 Frame = +3

Query: 72  EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGE 233
           E   E +EAF LFD    G I  ++L      LG+N T+ +LQ M++    +G+
Sbjct: 9   EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK 62


>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1011

 Score = 28.3 bits (60), Expect = 0.49
 Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
 Frame = +3

Query: 57  DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 218
           D+  E   A  +  +S+FD+D +G IT +E+      +G  +    A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593


>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
           zf-fungal binuclear cluster type |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 977

 Score = 26.6 bits (56), Expect = 1.5
 Identities = 9/27 (33%), Positives = 15/27 (55%)
 Frame = -2

Query: 201 LEVRLLWGSVLATSSRCPALWS*WCHR 121
           LE++  WGS+ A   +   +W+ W  R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876


>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 630

 Score = 25.4 bits (53), Expect = 3.5
 Identities = 9/24 (37%), Positives = 14/24 (58%)
 Frame = +3

Query: 93  EAFSLFDKDGDGTITTKELGTVMR 164
           + F  FD+D DG +  +EL  + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334


>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1250

 Score = 25.4 bits (53), Expect = 3.5
 Identities = 11/41 (26%), Positives = 22/41 (53%)
 Frame = +3

Query: 132 ITTKELGTVMRSLGQNPTEAELQDMINEVDADGEYT*NHMI 254
           ++ + L  ++ S  +NP E  LQ +  + +  G YT  H++
Sbjct: 212 VSGRSLRKLLSSSSKNPKEPRLQTIYLKTNKRGLYTLKHVV 252


>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1687

 Score = 24.6 bits (51), Expect = 6.1
 Identities = 10/37 (27%), Positives = 18/37 (48%)
 Frame = -3

Query: 116  FVEQ*ECLLKLGDLFFRQLIRHCRLTWIIWSYTKKSQ 6
            F E  EC+  L  ++    +  C+  W I+S+   S+
Sbjct: 1113 FCEPLECVETLVQIYLENNVECCQCIWDIFSFLSDSE 1149


>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 542

 Score = 24.2 bits (50), Expect = 8.0
 Identities = 9/33 (27%), Positives = 18/33 (54%)
 Frame = +3

Query: 99  FSLFDKDGDGTITTKELGTVMRSLGQNPTEAEL 197
           F    KD    +TTK++ T+  ++  +PT+  +
Sbjct: 321 FERLRKDSSLQLTTKDINTLFSTIALSPTKLSM 353


>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
           Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 24.2 bits (50), Expect = 8.0
 Identities = 20/57 (35%), Positives = 26/57 (45%)
 Frame = +3

Query: 30  DNPS*STMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQ 200
           +NP  ST    L E Q+        L D  GDG +T  E  ++ R L  N  +A LQ
Sbjct: 707 NNPDASTSVPVLEELQLRNKLFLTLLEDSIGDGDVTEDEKISIAR-LEANIDKALLQ 762


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,583,238
Number of Sequences: 5004
Number of extensions: 28427
Number of successful extensions: 69
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 142254980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)