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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ovS301D11f
         (502 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g53470.1 68414.m06061 mechanosensitive ion channel domain-con...    30   0.76 
At5g22890.1 68418.m02677 zinc finger (C2H2 type) family protein ...    28   3.1  
At3g14810.1 68416.m01871 mechanosensitive ion channel domain-con...    28   3.1  
At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein...    27   7.1  

>At1g53470.1 68414.m06061 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 881

 Score = 30.3 bits (65), Expect = 0.76
 Identities = 16/58 (27%), Positives = 29/58 (50%)
 Frame = -3

Query: 230 KYEIDTIPLYCKQLCKLPVTLIMMTDVKSNSMW*FKMYHIIISIY*KKCATVCLWTSL 57
           K+E+  + L C +L    +  + +  V+SN +W  K+ + +  I  +K    CLW  L
Sbjct: 294 KWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGI--RKPVQNCLWLGL 349


>At5g22890.1 68418.m02677 zinc finger (C2H2 type) family protein
           contains Pfam domain PF00096: Zinc finger, C2H2 type
          Length = 373

 Score = 28.3 bits (60), Expect = 3.1
 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -3

Query: 446 HEMVCSDMKWFLTVTRHMPRYNK-MHNYSILLGFAPSN 336
           HE  C D+KW  +      R +K M + S+ LG  P++
Sbjct: 325 HEKHCGDIKWVCSCGTKFSRKDKLMSHVSLFLGHVPAH 362


>At3g14810.1 68416.m01871 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 853

 Score = 28.3 bits (60), Expect = 3.1
 Identities = 14/58 (24%), Positives = 28/58 (48%)
 Frame = -3

Query: 230 KYEIDTIPLYCKQLCKLPVTLIMMTDVKSNSMW*FKMYHIIISIY*KKCATVCLWTSL 57
           K+E+  + L C +L    +  I++  V+ N  W  ++ + +  +  +K    CLW  L
Sbjct: 304 KWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGV--RKSVQNCLWLGL 359


>At1g66730.1 68414.m07585 ATP dependent DNA ligase family protein
           contains Pfam profile: PF01068 ATP dependent DNA ligase
           domain
          Length = 1417

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 11/26 (42%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
 Frame = +3

Query: 99  NTNYYVIHFKLPHR---ITFYISHHY 167
           NTN+ V  F+LPH+   + F++SH +
Sbjct: 62  NTNFIVDLFRLPHQSSSVAFFLSHFH 87


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,499,400
Number of Sequences: 28952
Number of extensions: 142661
Number of successful extensions: 247
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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