BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS301C09f (315 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 31 0.16 At5g56110.1 68418.m07000 myb family transcription factor contain... 30 0.37 At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 28 1.1 At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 27 2.0 At3g42460.1 68416.m04398 hypothetical protein several hypothetic... 27 3.5 At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, pu... 26 4.6 At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family pr... 26 4.6 At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family pr... 26 6.1 At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 26 6.1 At1g64880.1 68414.m07355 ribosomal protein S5 family protein con... 25 8.1 >At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase, putative / CoA-thioester hydrolase, putative strong similarity to gi:8572760; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 378 Score = 31.1 bits (67), Expect = 0.16 Identities = 13/23 (56%), Positives = 17/23 (73%) Frame = +1 Query: 226 VVGSKKNVGLIQLNRPKALNALC 294 +V K +V ++ LNRPK LNALC Sbjct: 8 LVEEKSSVRILTLNRPKQLNALC 30 >At5g56110.1 68418.m07000 myb family transcription factor contains PFAM profile: Myb DNA binding domain PF00249 Length = 320 Score = 29.9 bits (64), Expect = 0.37 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +2 Query: 122 CWERMY*TSAKWYPQQAKHL*SFIAQHHTRTSRL 223 C E+ +W P++ L S+IAQH TR RL Sbjct: 6 CCEKENVKRGQWTPEEDNKLASYIAQHGTRNWRL 39 >At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family protein similar to 3-hydroxybutyryl-CoA dehydratase (Crotonase) from Clostridium acetobutylicum [SP|P52046], FadB1x (enoyl-CoA hydratase) from Pseudomonas putida [GI:13310130]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein Length = 265 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/36 (33%), Positives = 23/36 (63%) Frame = +1 Query: 208 ENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFXEL 315 EN+ ++V + +I +NRPK+LN+L + + +L Sbjct: 7 ENL-IQVKKESGGIAVITINRPKSLNSLTRAMMVDL 41 >At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase / CoA-thioester hydrolase (CHY1) identical to gi:8572760; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 378 Score = 27.5 bits (58), Expect = 2.0 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = +1 Query: 226 VVGSKKNVGLIQLNRPKALNAL 291 +V K +V ++ LNRPK LNAL Sbjct: 12 LVEEKSSVRILTLNRPKQLNAL 33 >At3g42460.1 68416.m04398 hypothetical protein several hypothetical proteins - Arabidopsis thaliana Length = 202 Score = 26.6 bits (56), Expect = 3.5 Identities = 17/51 (33%), Positives = 27/51 (52%) Frame = +1 Query: 112 TRALLGKNVLNKCKVVSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQL 264 T A G N+++K V S IKF + S++NI + G+ +N L+ L Sbjct: 61 TSAEYGINLMDKTVVTSGDPNTPIKFNNFTSFDNI---LEGTFENDQLVDL 108 >At5g33280.1 68418.m03944 chloride channel-like (CLC) protein, putative similar to CLC-c, At5g49890 [Arabidopsis thaliana] and chloride channel protein ClC-1 - Nicotiana tabacum, PIR:T02939 Length = 763 Score = 26.2 bits (55), Expect = 4.6 Identities = 17/46 (36%), Positives = 20/46 (43%), Gaps = 1/46 (2%) Frame = +1 Query: 73 WFIFLKMASVATVTRALLGKNVLNKCKVVSATSQASIKFYS-TASY 207 W IF A VA V RAL+ + KC + YS ASY Sbjct: 263 WRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLIMFDVYSENASY 308 >At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family protein similar to CHY1 [gi:8572760]; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 418 Score = 26.2 bits (55), Expect = 4.6 Identities = 16/45 (35%), Positives = 25/45 (55%) Frame = +1 Query: 157 VSATSQASIKFYSTASYENIKVEVVGSKKNVGLIQLNRPKALNAL 291 +S + + ++ S E +K V + V LI L+RPKALNA+ Sbjct: 28 LSVSHRRKFSVMASGSDEFVKGNVYPN--GVALITLDRPKALNAM 70 >At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family protein similar to CHY1 [gi:8572760]; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 409 Score = 25.8 bits (54), Expect = 6.1 Identities = 17/41 (41%), Positives = 23/41 (56%) Frame = +1 Query: 193 STASYENIKVEVVGSKKNVGLIQLNRPKALNALCKPLFXEL 315 S A +E+ +V V G K+ I LN P +LNAL P+ L Sbjct: 35 SDADFED-QVLVEGKAKSRAAI-LNNPSSLNALSAPMVGRL 73 >At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase, putative / CoA-thioester hydrolase, putative strong similarity to gi:8572760; contains Pfam profile PF00388 enoyl-CoA hydratase/isomerase family protein Length = 422 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +1 Query: 226 VVGSKKNVGLIQLNRPKALNAL 291 +V K +V ++ NRPK LNAL Sbjct: 52 LVEEKSSVRILTFNRPKQLNAL 73 >At1g64880.1 68414.m07355 ribosomal protein S5 family protein contains similarity to 30S ribosomal protein S5 GI:6969105 from [Campylobacter jejuni] Length = 515 Score = 25.4 bits (53), Expect = 8.1 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +1 Query: 199 ASYENIKVEVVGSKKNVGLIQLNRPKALNALCKP 300 A ++NIK +V+GS+ + ++ KALNA+ P Sbjct: 466 AGFKNIKSKVIGSRNSYNTVKAVL-KALNAVETP 498 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,624,972 Number of Sequences: 28952 Number of extensions: 118072 Number of successful extensions: 280 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 277 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 280 length of database: 12,070,560 effective HSP length: 71 effective length of database: 10,014,968 effective search space used: 330493944 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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