BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS301C05f
(403 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC663.04 |rpl39||60S ribosomal protein L39|Schizosaccharomyces... 82 3e-17
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 42 5e-05
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 28 0.62
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 28 0.62
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 26 2.5
SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces... 25 3.3
SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual 25 4.4
SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2 |Schizosac... 25 5.8
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 5.8
SPAPB1A11.01 ||SPAPB24D3.11|membrane transporter|Schizosaccharom... 25 5.8
SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid aminotr... 24 7.7
>SPCC663.04 |rpl39||60S ribosomal protein L39|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 51
Score = 82.2 bits (194), Expect = 3e-17
Identities = 33/47 (70%), Positives = 40/47 (85%)
Frame = -1
Query: 193 KTFIIKRKLAKKLKQNRPIPQWVRMRTGNTIRYNAKRRHWRRTKLKL 53
K+F K+KLAK +QNRPIPQW+R+RTGNT+ YN KRRHWRRTKL +
Sbjct: 5 KSFRTKQKLAKAARQNRPIPQWIRLRTGNTVHYNMKRRHWRRTKLNI 51
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 41.5 bits (93), Expect = 5e-05
Identities = 17/33 (51%), Positives = 26/33 (78%)
Frame = +1
Query: 301 VLDLDLFRADKDGNPDKIRENQKKRFKDVALXD 399
+LD++LF+ +K GNP+ IRE+Q+KR DV + D
Sbjct: 1 MLDINLFQVEKGGNPEIIRESQRKRGADVGVVD 33
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 27.9 bits (59), Expect = 0.62
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 41 FTSLQLELCPSPVTPLSVISNSVSCAHPYPL 133
++S+ L +P TP S +N V CA P PL
Sbjct: 332 YSSIPLPAERNPKTPQSFSTNIVQCASPSPL 362
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.9 bits (59), Expect = 0.62
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2
Query: 71 SPVTPLSVISNSVSCAHPYPLRNGSVLFQLFGQFALNNKRLPVP 202
+P TP++V S++V+ AH Y GS + Q A N+ +P+P
Sbjct: 982 NPYTPIAVASSTVNPAHTYKPHGGSQIVPPPKQPA--NRVVPLP 1023
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.8 bits (54), Expect = 2.5
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = -3
Query: 356 RILSGLPSLSARNKSKS 306
R L GLPSLS RN KS
Sbjct: 130 RSLHGLPSLSRRNSKKS 146
>SPCC1840.08c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 25.4 bits (53), Expect = 3.3
Identities = 11/43 (25%), Positives = 19/43 (44%)
Frame = -1
Query: 247 LKLSNTSSLVSLVPIRHGKTFIIKRKLAKKLKQNRPIPQWVRM 119
+K N + L LV IRHG F + ++ + +W +
Sbjct: 277 IKTLNVTRLPHLVAIRHGVAFSYSERSVSAMRNTFQLIKWASL 319
>SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 973
Score = 25.0 bits (52), Expect = 4.4
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +2
Query: 83 PLSVISNSVSCAHPYPLRNGSVLFQLFGQFALNNKRLPVPNRH 211
P + SN VS ++ L S L GQF NN RH
Sbjct: 63 PPNSYSNGVSLSNGTLLSTSSPLISTSGQFTSNNVSTTSSQRH 105
>SPCC794.07 |||dihydrolipoamide S-acetyltransferase E2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 483
Score = 24.6 bits (51), Expect = 5.8
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +2
Query: 56 LELCPSPVTPLSVISNSVSCAHPYPLRNGSVLFQLFGQFALNNKRLP 196
+++ + TP +I+ + H L S L + +GQ A NNK P
Sbjct: 344 VDISMAVATPSGLITPVIRNTHALGLAEISTLAKDYGQRARNNKLKP 390
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 5.8
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = -3
Query: 260 VEIFFKIIKHVVSRLPRADSAREDV 186
+ + +K+IKH++ P A S R+ V
Sbjct: 213 INLEYKVIKHLLEHYPLALSVRQQV 237
>SPAPB1A11.01 ||SPAPB24D3.11|membrane
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 495
Score = 24.6 bits (51), Expect = 5.8
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -1
Query: 247 LKLSNTSSLVSLVPIRHGKTFIIKRKLAKKLKQNR 143
L LS S L +V ++ ++KRK AKKL + +
Sbjct: 202 LMLSGVSLLAGVVFLKETYAPVLKRKQAKKLLEKQ 236
>SPBC428.02c |eca39|SPBC582.12c|branched chain amino acid
aminotransferase Eca39|Schizosaccharomyces pombe|chr
2|||Manual
Length = 380
Score = 24.2 bits (50), Expect = 7.7
Identities = 8/32 (25%), Positives = 16/32 (50%)
Frame = -1
Query: 157 LKQNRPIPQWVRMRTGNTIRYNAKRRHWRRTK 62
+K+ +P+P+W ++ G + W R K
Sbjct: 23 VKELKPLPEWKSLKFGENFTDHMLIMKWNREK 54
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,587,480
Number of Sequences: 5004
Number of extensions: 30195
Number of successful extensions: 94
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 94
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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