BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= ovS301B12f (468 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g24610.1 68416.m03091 kelch repeat-containing F-box family pr... 31 0.51 At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive eff... 29 2.1 At2g40360.1 68415.m04977 transducin family protein / WD-40 repea... 29 2.1 At2g19120.1 68415.m02232 tRNA-splicing endonuclease positive eff... 29 2.1 At3g19130.1 68416.m02429 RNA-binding protein, putative similar t... 28 3.6 At1g27270.1 68414.m03322 paired amphipathic helix repeat-contain... 28 3.6 At1g03080.1 68414.m00282 kinase interacting family protein simil... 28 3.6 >At3g24610.1 68416.m03091 kelch repeat-containing F-box family protein contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 445 Score = 30.7 bits (66), Expect = 0.51 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -1 Query: 141 EVSVIQDRIWIFGGMSYKEHFDVSNVF 61 EVSV+ +I ++GG YK ++D VF Sbjct: 201 EVSVVDGKINVWGGCKYKHYYDWGEVF 227 >At4g30100.1 68417.m04280 tRNA-splicing endonuclease positive effector-related contains similarity to SEN1, a positive effector of tRNA-splicing endonuclease [Saccharomyces cerevisiae] gi|172574|gb|AAB63976 Length = 1311 Score = 28.7 bits (61), Expect = 2.1 Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = -1 Query: 399 EQEASSDTRFTNSEDQVFDSTRSD-RKIEQRLLAGFDVV 286 E AS + F N + VF + S RK+ RL GFD+V Sbjct: 903 EARASLEASFANEAEIVFTTVSSSGRKLFSRLTHGFDMV 941 >At2g40360.1 68415.m04977 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to block of proliferation protein Bop1 (GI:1679772) [Mus musculus] Length = 753 Score = 28.7 bits (61), Expect = 2.1 Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 3/67 (4%) Frame = +1 Query: 55 KSKHITDV---KVFLVGHTSKYPYPILYDTDLKLKNAHVHFDDKERYNNIPTIKTGYESF 225 KSKH+T + K+ L GH Y + Y + K ++ +++R IPT T S Sbjct: 304 KSKHLTYIPPPKLKLPGHDESYNPSLEYIPTEEEKASYELMFEEDRPKFIPTRFTSLRSI 363 Query: 226 DKYEDQI 246 YE+ + Sbjct: 364 PAYENAL 370 >At2g19120.1 68415.m02232 tRNA-splicing endonuclease positive effector-related similar to Endonuclease sen1 (Swiss-Prot:Q92355) [Schizosaccharomyces pombe]; similar to tRNA-splicing endonuclease positive effector (Swiss-Prot:Q00416) [Saccharomyces cerevisiae] Length = 1090 Score = 28.7 bits (61), Expect = 2.1 Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = -1 Query: 399 EQEASSDTRFTNSEDQVFDSTRSD-RKIEQRLLAGFDVV 286 E AS + F N + VF + S RK+ RL GFD+V Sbjct: 740 EARASLEASFANEAEIVFTTVSSSGRKLFSRLTHGFDMV 778 >At3g19130.1 68416.m02429 RNA-binding protein, putative similar to RNA Binding Protein 47 [Nicotiana plumbaginifolia] GI:9663769, DNA binding protein ACBF GB:AAC49850 from [Nicotiana tabacum]; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 435 Score = 27.9 bits (59), Expect = 3.6 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +3 Query: 234 RGPNHQYHQRLEKYIWIQQHRSQQ 305 +GP Q+H +L +Y QQH+ QQ Sbjct: 72 QGPYQQHHPQLHQYGSYQQHQHQQ 95 >At1g27270.1 68414.m03322 paired amphipathic helix repeat-containing protein contains Pfam profile PF02671: Paired amphipathic helix repeat Length = 241 Score = 27.9 bits (59), Expect = 3.6 Identities = 15/58 (25%), Positives = 27/58 (46%) Frame = +1 Query: 142 KLKNAHVHFDDKERYNNIPTIKTGYESFDKYEDQIISIINDLKNTYGFNNIEASKQSL 315 K K+ +F+D Y + + +KY++ + I+ N +GF + AS Q L Sbjct: 4 KSKSPEFNFEDGMAYFDAVKVALQDTEPEKYQEFVRIFIDYTANRFGFETLSASLQEL 61 >At1g03080.1 68414.m00282 kinase interacting family protein similar to kinase interacting protein 1 (GI:13936326) [Petunia integrifolia] Length = 1744 Score = 27.9 bits (59), Expect = 3.6 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 2/62 (3%) Frame = +1 Query: 136 DLKLKNAHVHFDDKERYNNIPTIKTGYESFDKYEDQIISIINDL--KNTYGFNNIEASKQ 309 DLK+K D+K + N TIK ++ ++++SI L K GF + SK Sbjct: 1589 DLKIKVETEEKDEKGKENEYETIKGQINEAEEALEKLLSINRKLVTKVQNGFERSDGSKS 1648 Query: 310 SL 315 S+ Sbjct: 1649 SM 1650 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,446,149 Number of Sequences: 28952 Number of extensions: 214147 Number of successful extensions: 621 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 611 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 621 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 791932800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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