Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= ovS301A12f
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative s... 49 2e-06
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 48 5e-06
At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative... 45 3e-05
At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative... 43 1e-04
At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative... 40 0.001
At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative... 38 0.003
At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative... 35 0.028
At3g08490.1 68416.m00984 hypothetical protein 33 0.11
At3g53810.1 68416.m05945 lectin protein kinase, putative similar... 27 9.9
>At1g65560.1 68414.m07437 allyl alcohol dehydrogenase, putative
similar to allyl alcohol dehydrogenase from Nicotiana
tabacum [gi:6692816]; similar to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 350
Score = 49.2 bits (112), Expect = 2e-06
Identities = 34/125 (27%), Positives = 58/125 (46%), Gaps = 13/125 (10%)
Frame = +2
Query: 176 NMVIAKKIIVVNPFVGVPKHNDFDI----VXXXXXXXXXXXFLAEAIYFSVDPYQRMKL- 340
++V KK+I+ N G+P D ++ FL + +Y S DPY R ++
Sbjct: 5 SVVENKKVILKNYVDGIPTETDMEVKLGETIELKAPKGSSCFLVKNLYLSCDPYMRGRMR 64
Query: 341 ---GNVYPCDMIGGQI-----ARVIESKNTEYPVGTLVMGHFGWRTHTITKPENESSVNK 496
G+ P + G +I ARVI+S +T Y G +V G GW +++ + + +
Sbjct: 65 DFHGSYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQLRN 124
Query: 497 TLLDN 511
LD+
Sbjct: 125 IQLDD 129
>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 345
Score = 47.6 bits (108), Expect = 5e-06
Identities = 30/109 (27%), Positives = 49/109 (44%), Gaps = 15/109 (13%)
Frame = +2
Query: 191 KKIIVVNPFVGVPKHNDFDI---VXXXXXXXXXXXFLAEAIYFSVDPYQRMKLGNVYPCD 361
K++++ N G PK +DFD L + +Y S DPY R+++G P
Sbjct: 6 KQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYMRIRMGKPDPST 65
Query: 362 ------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
+ G ++RVIES + +Y G L+ G GW +++ P
Sbjct: 66 AALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITP 114
>At5g16990.1 68418.m01990 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 343
Score = 44.8 bits (101), Expect = 3e-05
Identities = 30/112 (26%), Positives = 48/112 (42%), Gaps = 14/112 (12%)
Frame = +2
Query: 179 MVIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNV 349
M K++I + G PK +DF+ L + +Y S DPY R ++G
Sbjct: 1 MTTNKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKP 60
Query: 350 YPCDMI-----------GGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
P + G ++RVIES + +Y G L+ G GW +++ P
Sbjct: 61 DPSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITP 112
>At3g03080.1 68416.m00304 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana; similar to allyl alcohol
dehydrogenase [Nicotiana tabacum] GI:6692816
Length = 350
Score = 43.2 bits (97), Expect = 1e-04
Identities = 29/116 (25%), Positives = 52/116 (44%), Gaps = 16/116 (13%)
Frame = +2
Query: 176 NMVIAKKIIVVNPFV-GVPKHNDF---DIVXXXXXXXXXXXFLAEAIYFSVDPYQRMKLG 343
N +A K +++ +V G PK +D D L + +Y S DPY R+++G
Sbjct: 6 NATVANKQVILRDYVTGFPKESDLIFTDSTIDLKIPEGSKTVLVKNLYLSCDPYMRIRMG 65
Query: 344 NVYPC------------DMIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKPE 475
P + G +++VI+S + +Y G L+ G GW +++ P+
Sbjct: 66 KPDPGTAALAPHYIPGEPIYGFSVSKVIDSGHPDYKKGDLLWGLVGWGEYSLITPD 121
>At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative
(P1) identical to probable NADP-dependent oxidoreductase
P1, zeta-crystallin homolog [SP|Q39172][gi:886428],
Arabidopsis thaliana; similar to allyl alcohol
dehydrogenase [Nicotiana tabacum] GI:6692816; contains
Pfam profile PF00107: oxidoreductase, zinc-binding
dehydrogenase family
Length = 345
Score = 39.9 bits (89), Expect = 0.001
Identities = 28/114 (24%), Positives = 48/114 (42%), Gaps = 16/114 (14%)
Frame = +2
Query: 179 MVIAKKIIVVNPFV-GVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGN 346
M K +++ +V G P +DFD L + +Y S DPY R+++G
Sbjct: 1 MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGK 60
Query: 347 VYPC------------DMIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
P + G ++R+IES + +Y G L+ G W +++ P
Sbjct: 61 PDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITP 114
>At1g26320.1 68414.m03210 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana; similar
to allyl alcohol dehydrogenase GI:9758497 from
[Arabidopsis thaliana]
Length = 351
Score = 38.3 bits (85), Expect = 0.003
Identities = 29/109 (26%), Positives = 47/109 (43%), Gaps = 15/109 (13%)
Frame = +2
Query: 182 VIAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXX---FLAEAIYFSVDPYQRMKLGNVY 352
V K+II + G PK +D I L + +Y S DPY R+ +G
Sbjct: 9 VTNKQIIFPDYVTGFPKESDLKITTTTIDLRLPEGSTSVLVKNLYLSCDPYMRICMGKPD 68
Query: 353 PCD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTI 463
P +IG +++VI+S + +Y G L+ G GW +++
Sbjct: 69 PLSSSLVPPYKTGVPIIGLGVSKVIDSGHPDYKKGDLLWGLVGWEEYSV 117
>At5g16960.1 68418.m01987 NADP-dependent oxidoreductase, putative
similar to probable NADP-dependent oxidoreductase
(zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and
P2 [SP|Q39173][gi:886430], Arabidopsis thaliana
Length = 346
Score = 35.1 bits (77), Expect = 0.028
Identities = 25/111 (22%), Positives = 48/111 (43%), Gaps = 15/111 (13%)
Frame = +2
Query: 185 IAKKIIVVNPFVGVPKHNDFDIVXXXXXXXXXXXF---LAEAIYFSVDPYQRMKLGNVYP 355
I K++++++ G K +D I L + +Y S DPY R ++ P
Sbjct: 5 INKQVVLLDYVTGFAKESDLVITSTTIDLRVLKGSMTALVKNLYLSCDPYMRNRMRKPDP 64
Query: 356 CD------------MIGGQIARVIESKNTEYPVGTLVMGHFGWRTHTITKP 472
+ G +++VI+S +++Y G L+ G GW +++ P
Sbjct: 65 LSPATAQSFTPGKPISGFGVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITP 115
>At3g08490.1 68416.m00984 hypothetical protein
Length = 271
Score = 33.1 bits (72), Expect = 0.11
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -2
Query: 386 LWQFVHQSYHKDIHCLVSFFDKDRH*SILLLLETHFLV 273
+W ++ YH + CL+ DK+RH + ++ E LV
Sbjct: 233 VWNIINPKYHHKVECLLLLADKERHSHVTMIREKCRLV 270
>At3g53810.1 68416.m05945 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 677
Score = 26.6 bits (56), Expect = 9.9
Identities = 10/21 (47%), Positives = 16/21 (76%)
Frame = -3
Query: 421 PNRILGIFTFNNSGNLSTNHI 359
P++ +G+F +N+GN TNHI
Sbjct: 120 PSQYIGLFNISNNGN-DTNHI 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,318,679
Number of Sequences: 28952
Number of extensions: 189011
Number of successful extensions: 417
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 412
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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