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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV1054.Seq
         (399 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces...    27   1.4  
SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosa...    25   5.7  
SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharom...    25   5.7  
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma...    24   7.6  
SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual       24   7.6  
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    24   10.0 
SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|ch...    24   10.0 
SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces ...    24   10.0 
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo...    24   10.0 

>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 471

 Score = 26.6 bits (56), Expect = 1.4
 Identities = 10/44 (22%), Positives = 26/44 (59%)
 Frame = +2

Query: 26  TNPHRLRRTSKSREIIQGNNYIVQYXWFCMGLYSSAILAVSVYS 157
           T+P+ L + ++   +++ N Y+VQ     + LY++++ +   Y+
Sbjct: 261 THPNVLDKWNEFISLLKSNGYLVQEIQLPISLYANSVYSTMAYA 304


>SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase
           Gmh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 329

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 9/24 (37%), Positives = 16/24 (66%)
 Frame = +3

Query: 279 RSEIHYLKICLYNFYFSNISNKLI 350
           ++ I Y K+  YNF F N+S+ ++
Sbjct: 110 KNRIEYAKLQNYNFEFVNVSSLVV 133


>SPBC16C6.06 |pep1|vps10|sorting receptor for
           CPY|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1466

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 9/21 (42%), Positives = 16/21 (76%)
 Frame = +3

Query: 9   DFFTSIPTHIDYVGQAKAEKL 71
           D ++ +P++++Y   AKAEKL
Sbjct: 843 DTWSRLPSNLEYCSWAKAEKL 863


>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 629

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +3

Query: 45  VGQAKAEKLYRAIITLFSIXGFVWGYI 125
           V  ++   L  A + LF I GFV GY+
Sbjct: 357 VAPSRRGSLATATVALFIISGFVSGYV 383


>SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 111

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 12/46 (26%), Positives = 22/46 (47%)
 Frame = +2

Query: 59  SREIIQGNNYIVQYXWFCMGLYSSAILAVSVYSWCWISACCHSYCS 196
           +R +++ N Y +    F + +    +L V +  +  I  CC S CS
Sbjct: 14  NRNLLEANKYKLSEITFKVYISIRRLLIVVITLYRRIVECCASLCS 59


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 2685

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +2

Query: 5    DGFLYIYTNPHRLRRTSKSREIIQGNNY 88
            DGF +IY+  H  R+ + S+     +NY
Sbjct: 2377 DGFPHIYSRNHDKRKENGSQGEADNSNY 2404


>SPBC17G9.08c |csx2||Arf GAP protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 870

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 12/26 (46%), Positives = 19/26 (73%)
 Frame = -2

Query: 230 VQRIASVHWPWRNSKNGSKQKSSTKN 153
           +QR+ASV  P R++ + SK+K  TK+
Sbjct: 645 LQRVASVTSPSRHNSD-SKEKKQTKS 669


>SPAC1486.03c |||RNA-binding splicing factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 797

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = +1

Query: 235 RFLRTVIQTSSQERKEVKFIIS 300
           RFL ++I  S  + KE++F+ S
Sbjct: 216 RFLASLIDASLNDTKEIEFVTS 237


>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
           Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 254

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 8/16 (50%), Positives = 9/16 (56%)
 Frame = +2

Query: 164 WISACCHSYCSSMANV 211
           W + C H YC S A V
Sbjct: 208 WATKCGHVYCGSCAKV 223


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,474,864
Number of Sequences: 5004
Number of extensions: 26507
Number of successful extensions: 74
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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