Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1020.Seq
(536 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB17E12.13 |rpl1802|rpl18-2|60S ribosomal protein L18|Schizos... 102 4e-23
SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S... 102 4e-23
SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces po... 29 0.44
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.0
SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransfer... 28 1.0
SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr ... 28 1.0
SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces ... 27 1.3
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 27 1.8
SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces... 27 1.8
SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 26 3.1
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 26 3.1
>SPAPB17E12.13 |rpl1802|rpl18-2|60S ribosomal protein
L18|Schizosaccharomyces pombe|chr 1|||Manual
Length = 187
Score = 102 bits (244), Expect = 4e-23
Identities = 51/86 (59%), Positives = 63/86 (73%)
Frame = +2
Query: 242 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 421
G +D RL +PK++VAAL T+ ARARIL AGGE+LT DQLALRAPTG TVL++G++
Sbjct: 86 GTVTDDERLLTVPKLSVAALRFTKSARARILKAGGEVLTLDQLALRAPTGSNTVLLRGKK 145
Query: 422 NAREAARHFGPAPGAPXSHXKPYVRT 499
+AREA RHFG P H PYVR+
Sbjct: 146 HAREAYRHFG---FGPHKHKAPYVRS 168
Score = 62.9 bits (146), Expect = 3e-11
Identities = 32/86 (37%), Positives = 50/86 (58%), Gaps = 2/86 (2%)
Frame = +3
Query: 3 DINHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPISV 182
DI H RK +R++ S+++ T+++FN+ +L+RLF S+ NRPPIS+
Sbjct: 4 DIERHHVRKSQRSKPASENVYLKLLVKLYRFLARRTDSRFNKAILKRLFQSKTNRPPISI 63
Query: 183 SRLA--RHMKKPTREGLIAVVVGTVT 254
S++A K + EG V+VGTVT
Sbjct: 64 SKIAALTSRKSASLEGKTTVIVGTVT 89
>SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein
L18|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 102 bits (244), Expect = 4e-23
Identities = 50/86 (58%), Positives = 63/86 (73%)
Frame = +2
Query: 242 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 421
G +D R+ +PK+++AAL T+ ARARIL AGGE+LT DQLALRAPTG TVLV+G++
Sbjct: 86 GTVTDDERMLTVPKLSIAALRFTKSARARILKAGGEVLTLDQLALRAPTGSNTVLVRGKK 145
Query: 422 NAREAARHFGPAPGAPXSHXKPYVRT 499
+AREA RHFG P H PYVR+
Sbjct: 146 HAREAYRHFG---FGPHKHKAPYVRS 168
Score = 58.8 bits (136), Expect = 5e-10
Identities = 30/86 (34%), Positives = 49/86 (56%), Gaps = 2/86 (2%)
Frame = +3
Query: 3 DINHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPISV 182
DI H +K +R++ S+++ T+++FN+ +L+RLF S+ NRPPIS+
Sbjct: 4 DIERHHVKKSQRSKPASENVYLKLLVKLYRFLARRTDSRFNKAILKRLFQSKTNRPPISI 63
Query: 183 SRLA--RHMKKPTREGLIAVVVGTVT 254
S++A K + + VVVGTVT
Sbjct: 64 SKIAALTSRKSASSQNKTTVVVGTVT 89
>SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 427
Score = 29.1 bits (62), Expect = 0.44
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = -2
Query: 355 KNFSSSSQNACTSFFGNMKSSHRHLRYLVQSHVIVTVPT 239
KN+ SS + TS + N+ +S+R +R +QS V + PT
Sbjct: 170 KNWLSSELSHSTSKYLNITTSNRFIRKAIQSLVKIEFPT 208
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.0
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = -2
Query: 304 MKSSHRHLRYLVQSHVIVTVPTTTAIKPSRVGFFMWRAKRDTEIGGRLIRL 152
+K+S HL +Q +++ + P + +++WR + E +IRL
Sbjct: 231 LKASRIHLETSIQPKLMLLTGIILLVAPILLSYYVWRRRIHFETSFNIIRL 281
>SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransferase
Mam4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 236
Score = 27.9 bits (59), Expect = 1.0
Identities = 27/106 (25%), Positives = 44/106 (41%), Gaps = 1/106 (0%)
Frame = -2
Query: 457 SRAKVTRCLTSISLTLXQYCLLASRSTKSQLIKSKNFSSSSQNACTSFFGNMKSSHRHLR 278
S AKV CL S + + S L + SS+ F ++ +S R+
Sbjct: 95 SFAKVINCLRFPSFLINFIFSVYQTSALGFLCLGQYLRSSAMVQAGQSFSHIVASKRN-- 152
Query: 277 YLVQSHVIVTVPTTTAIK-PSRVGFFMWRAKRDTEIGGRLIRLIKS 143
+ H++VT ++ PS VGFF+W +G + L+ S
Sbjct: 153 ---KDHLLVTDGIYAYVRHPSYVGFFIWALGTQMLLGNFVSTLLFS 195
>SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 495
Score = 27.9 bits (59), Expect = 1.0
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 257 DVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQL 370
D+ Y+IP + + + TE+A+ R G+IL D +
Sbjct: 59 DLLFYEIPFLLIKHIENTEEAKLRFALPQGQILEIDTI 96
>SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 504
Score = 27.5 bits (58), Expect = 1.3
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -2
Query: 358 SKNFSSSSQNACTSFFGNMKSSHRH 284
SKN SS N+ TSFF ++ + +RH
Sbjct: 2 SKNSFSSMANSVTSFFQSLTTPNRH 26
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 27.1 bits (57), Expect = 1.8
Identities = 18/65 (27%), Positives = 30/65 (46%)
Frame = +2
Query: 248 SHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQRNA 427
+H D L +P + VT R + GG + R+ T ++T+L G RN+
Sbjct: 58 NHLDANLSNVPTINSIPSPVT---RPPVAVGGGIFYGARRTRNRSQTQRRTLLENGFRNS 114
Query: 428 REAAR 442
R+ A+
Sbjct: 115 RKKAQ 119
>SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 475
Score = 27.1 bits (57), Expect = 1.8
Identities = 16/58 (27%), Positives = 30/58 (51%)
Frame = -2
Query: 403 YCLLASRSTKSQLIKSKNFSSSSQNACTSFFGNMKSSHRHLRYLVQSHVIVTVPTTTA 230
Y + +ST + + S F+ +QN+ + F N +S + + Q++ I PTT+A
Sbjct: 160 YKSIFDKSTSNSTVTSNQFNKPTQNSPFNSFSNNNNSFNNNQ---QANDIFGAPTTSA 214
>SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4
desaturase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 362
Score = 26.2 bits (55), Expect = 3.1
Identities = 10/41 (24%), Positives = 21/41 (51%)
Frame = -1
Query: 326 VHELFR*HEEQPPSSSVSCTVSRHCDCPHYHGNQTLTSWLL 204
VH+ + + E+P S + + H + +G + LT W++
Sbjct: 26 VHQFYWTYTEEPHKSRRAAILKAHPEIASLNGYEPLTKWIV 66
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 26.2 bits (55), Expect = 3.1
Identities = 21/77 (27%), Positives = 33/77 (42%)
Frame = -2
Query: 463 SWSRAKVTRCLTSISLTLXQYCLLASRSTKSQLIKSKNFSSSSQNACTSFFGNMKSSHRH 284
S S + + TS S + +S S+ S S + SSSS + +S + +SHR
Sbjct: 122 SSSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSSRSSSRTTSHRT 181
Query: 283 LRYLVQSHVIVTVPTTT 233
+ S+ P TT
Sbjct: 182 TSHKSSSYRPTVFPYTT 198
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,084,598
Number of Sequences: 5004
Number of extensions: 40811
Number of successful extensions: 128
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -