Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1020.Seq
(536 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) simi... 119 1e-27
At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S ... 116 8e-27
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 109 1e-24
At2g02640.1 68415.m00203 DC1 domain-containing protein contain... 31 0.49
At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to hom... 30 1.1
At1g49920.1 68414.m05598 zinc finger protein-related weak simila... 29 2.0
At1g05440.1 68414.m00552 expressed protein ; expression supporte... 29 2.0
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf... 28 3.4
At5g08260.1 68418.m00971 serine carboxypeptidase S10 family prot... 28 4.5
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 28 4.5
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 28 4.5
At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin fa... 27 6.0
At1g32190.1 68414.m03959 expressed protein 27 6.0
>At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) similar
to GB:P42791
Length = 187
Score = 119 bits (287), Expect = 1e-27
Identities = 55/85 (64%), Positives = 67/85 (78%)
Frame = +2
Query: 242 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 421
G +D+R+++IP M V AL TE+ARARI AGGE LTFDQLALRAP G+ TVL++G +
Sbjct: 83 GTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNTVLLRGPK 142
Query: 422 NAREAARHFGPAPGAPXSHXKPYVR 496
N+REA +HFGPAPG P SH KPYVR
Sbjct: 143 NSREAVKHFGPAPGVPHSHSKPYVR 167
Score = 64.1 bits (149), Expect = 6e-11
Identities = 32/77 (41%), Positives = 47/77 (61%)
Frame = +3
Query: 24 RKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPISVSRLARHM 203
+K +RT KS D+ TN+KFN ++L+RLFMS++N+ P+S+SRL M
Sbjct: 12 KKTKRTAPKSDDVYLKLTVKLYRFLVRRTNSKFNGVILKRLFMSKVNKAPLSLSRLVEFM 71
Query: 204 KKPTREGLIAVVVGTVT 254
+E IAV+VGT+T
Sbjct: 72 --TGKEDKIAVLVGTIT 86
>At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S
ribosomal protein L18, Arabidopsis thaliana,
SWISSPROT:RL18_ARATH
Length = 187
Score = 116 bits (280), Expect = 8e-27
Identities = 54/85 (63%), Positives = 67/85 (78%)
Frame = +2
Query: 242 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 421
G +D+R+++IP M V AL TE+ARARI AGGE LTFDQLALRAP G+ TVL++G +
Sbjct: 83 GTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNTVLLRGPK 142
Query: 422 NAREAARHFGPAPGAPXSHXKPYVR 496
N+REA +HFGPAPG P S+ KPYVR
Sbjct: 143 NSREAVKHFGPAPGVPHSNTKPYVR 167
Score = 59.7 bits (138), Expect = 1e-09
Identities = 29/77 (37%), Positives = 46/77 (59%)
Frame = +3
Query: 24 RKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPISVSRLARHM 203
+K +RT KS D+ +N+ FN ++L+RLFMS++N+ P+S+SRL M
Sbjct: 12 KKTKRTAPKSDDVYLKLLVKLYRFLVRRSNSNFNAVILKRLFMSKVNKAPLSLSRLVEFM 71
Query: 204 KKPTREGLIAVVVGTVT 254
++ IAV+VGT+T
Sbjct: 72 --TGKDDKIAVLVGTIT 86
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 109 bits (262), Expect = 1e-24
Identities = 53/86 (61%), Positives = 62/86 (72%), Gaps = 1/86 (1%)
Frame = +2
Query: 242 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPT-GKKTVLVQGQ 418
G +DVR+ +P +TV AL TE ARARI AGGE LTFDQLAL PT + TVL++G
Sbjct: 30 GTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPTWSENTVLLRGP 89
Query: 419 RNAREAARHFGPAPGAPXSHXKPYVR 496
+N REA +HFGPAPG P SH KPYVR
Sbjct: 90 KNTREAVKHFGPAPGVPHSHTKPYVR 115
Score = 40.7 bits (91), Expect = 6e-04
Identities = 19/35 (54%), Positives = 28/35 (80%)
Frame = +3
Query: 150 MSRINRPPISVSRLARHMKKPTREGLIAVVVGTVT 254
MS++N+ P+S+SRL R+M ++G IAV+VGTVT
Sbjct: 1 MSKVNKAPLSLSRLVRYM--DGKDGKIAVIVGTVT 33
>At2g02640.1 68415.m00203 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 31.1 bits (67), Expect = 0.49
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 295 CSSCYRKSSCTHFGCWRRNSYF 360
CS+C RKS+ + C+ RN YF
Sbjct: 423 CSACMRKSNGFGYSCYNRNCYF 444
>At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to
homeodomain transcription factor (AGL30) GI:3461830 from
[Arabidopsis thaliana]; contains Pfam domain PF00319:
SRF-type transcription factor (DNA-binding and
dimerisation domain); PMID: 12837945
Length = 389
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = -1
Query: 452 GQSDALPHEHFADLXPVLSSCQSEHEEPADQ 360
G S LPH PV SSC E +P DQ
Sbjct: 223 GDSSFLPHREMDGSIPVYSSCFFESTKPEDQ 253
>At1g49920.1 68414.m05598 zinc finger protein-related weak
similarity to mudrA [Zea mays] GI:540581, MURAZC [Zea
mays] GI:1857256; contains Pfam profiles PF03108: MuDR
family transposase, PF04434: SWIM zinc finger
Length = 785
Score = 29.1 bits (62), Expect = 2.0
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 248 SHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQL 370
+H+D R Y I ++ AL K ++ AGG +L F QL
Sbjct: 537 AHDDGRRYGIMRIDTEALFAVCKRFRKVAMAGGVMLLFGQL 577
>At1g05440.1 68414.m00552 expressed protein ; expression supported
by MPSS
Length = 393
Score = 29.1 bits (62), Expect = 2.0
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -2
Query: 436 CLTSISLTLXQYCLLASRSTKSQLIKSKNFSSSSQNACTSF 314
CL S++ TL L +S+ ++L + NF S+ ++ TSF
Sbjct: 71 CLDSLNATLDLMPLSVQKSSLTKLSSASNFKSTVESTPTSF 111
>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 883
Score = 28.3 bits (60), Expect = 3.4
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
Frame = +1
Query: 259 RETVQDTEDDGGCSSCY--RKSSCTHFGCWRRNSYF*SAGSSCS 384
R+ + +E GGC+SC KS T C R+ + S G+ CS
Sbjct: 145 RDHGECSESVGGCASCIVRSKSDITTSQCGDRDRRYTSPGNPCS 188
>At5g08260.1 68418.m00971 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)];
carboxypeptidase D - Triticum aestivum, PIR:A29639
Length = 480
Score = 27.9 bits (59), Expect = 4.5
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = +2
Query: 233 RGSGDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTG 391
+G D++ND+ +Y I + ++ R + +LTFD L + P G
Sbjct: 275 KGFMDAYNDIDIYSIYTPVCLSSLLSSSPRKPKIVVSPRLLTFDDLWDKFPAG 327
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +3
Query: 123 NQIVLRRLFMSRINRPPISVSRLARHMKKPTREGLI 230
+Q V + S INRPP+ + H R+GL+
Sbjct: 34 SQTVHSHAYHSGINRPPVETKPVTEHKSFTRRDGLL 69
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 347 EILTFDQLALRAPTGKKTVLVQGQRNAR 430
E L FD L L+ P+GK LV G + +
Sbjct: 290 ETLIFDDLCLKIPSGKTVALVGGSGSGK 317
>At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin
family protein contains Pfam profile PF01190: Pollen
proteins Ole e I family
Length = 401
Score = 27.5 bits (58), Expect = 6.0
Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 2/48 (4%)
Frame = -2
Query: 448 KVTRCLTSISLTLXQYCLLASRSTKSQL--IKSKNFSSSSQNACTSFF 311
K+ RC + + YC +AS +T S L +KS + +++ FF
Sbjct: 108 KIKRCSVKLLSSSQPYCSIASSATSSSLKRLKSNHHGENTRVFSAGFF 155
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 27.5 bits (58), Expect = 6.0
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = +1
Query: 295 CSSCYRKSSCTHFGCWR 345
CSSC+ K C CW+
Sbjct: 359 CSSCFGKPKCPKCSCWK 375
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,280,532
Number of Sequences: 28952
Number of extensions: 231502
Number of successful extensions: 684
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 681
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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