Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1019.Seq
(588 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04670.1 68418.m00475 expressed protein BRL protein, Homo sap... 33 0.14
At2g22795.1 68415.m02704 expressed protein 33 0.19
At5g46760.1 68418.m05760 basic helix-loop-helix (bHLH) family pr... 31 0.75
At3g28770.1 68416.m03591 expressed protein 31 0.75
At1g17140.2 68414.m02089 tropomyosin-related similar to Tropomyo... 29 1.7
At1g17140.1 68414.m02088 tropomyosin-related similar to Tropomyo... 29 1.7
At1g56660.1 68414.m06516 expressed protein 29 2.3
At5g64530.1 68418.m08110 no apical meristem (NAM) family protein... 29 3.0
At3g52770.1 68416.m05814 expressed protein emm32, Streptococcus ... 28 4.0
At5g55300.1 68418.m06891 DNA topoisomerase I identical to Swiss-... 28 5.3
At4g33200.1 68417.m04727 myosin, putative similar to myosin (GI:... 28 5.3
At1g77580.2 68414.m09032 myosin heavy chain-related low similari... 28 5.3
At1g77580.1 68414.m09033 myosin heavy chain-related low similari... 28 5.3
At1g65660.1 68414.m07450 zinc knuckle (CCHC-type) family protein... 28 5.3
At5g25870.1 68418.m03069 hypothetical protein 27 7.0
At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase fa... 27 7.0
At5g28290.1 68418.m03434 protein kinase, putative similar to LST... 27 9.3
At4g37120.1 68417.m05257 expressed protein 27 9.3
At1g33390.1 68414.m04133 helicase domain-containing protein simi... 27 9.3
>At5g04670.1 68418.m00475 expressed protein BRL protein, Homo
sapiens, EMBL:AF005067
Length = 766
Score = 33.1 bits (72), Expect = 0.14
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = -2
Query: 425 DAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEPKCENKLIQKFSDSENNSI 264
D+ LKN+NK+ NK + K+N Q CS ++ + E K ++ + K N +
Sbjct: 52 DSVLKNQNKSKGNKVSSGKSNSQPCSPKQVSSEKEDKVDDFPVTKRRKVRNEGV 105
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 32.7 bits (71), Expect = 0.19
Identities = 21/90 (23%), Positives = 44/90 (48%), Gaps = 3/90 (3%)
Frame = -2
Query: 497 KSNKKVLKKAKISNKENTYKNTKS---DAALKNENKNSINKSLNTKANLQNCSIEKDNFT 327
K N+K+ K+ S +E K T++ + + NE++ ++N K ++ + D T
Sbjct: 599 KENEKIEKEESASQEETKEKETETKEKEESSSNESQENVNTESEKKEQVEENEKKTDEDT 658
Query: 326 SEPKCENKLIQKFSDSENNSITSHRMDTLK 237
SE EN + ++ + + TS + ++ K
Sbjct: 659 SESSKENSVSD--TEQKQSEETSEKEESNK 686
Score = 27.5 bits (58), Expect = 7.0
Identities = 18/76 (23%), Positives = 32/76 (42%), Gaps = 1/76 (1%)
Frame = -2
Query: 497 KSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKD-NFTSE 321
++ KK + S+KEN+ +T+ + + K NK+ T+ + D N E
Sbjct: 649 ENEKKTDEDTSESSKENSVSDTEQKQSEETSEKEESNKNGETEVTQEQSDSSSDTNLPQE 708
Query: 320 PKCENKLIQKFSDSEN 273
K ++ DS N
Sbjct: 709 VKDVRTDLETLPDSGN 724
>At5g46760.1 68418.m05760 basic helix-loop-helix (bHLH) family
protein
Length = 592
Score = 30.7 bits (66), Expect = 0.75
Identities = 16/54 (29%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = -2
Query: 494 SNKKVLKKAKISNKENTYKNTKSDA-ALKNENKNSINKSLNTKANLQNCSIEKD 336
+N + A+++N N+ N+KSD+ + KN I+ S+ + +C +EKD
Sbjct: 272 TNTGIESPARVNNGNNSNSNSKSDSHQISKLEKNDIS-SVENQNRQSSCLVEKD 324
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 30.7 bits (66), Expect = 0.75
Identities = 19/89 (21%), Positives = 39/89 (43%)
Frame = -2
Query: 497 KSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEP 318
+ +KK KK + S ++ K L+++N N + N K Q+ + K +
Sbjct: 1096 EEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKKESDKKE 1155
Query: 317 KCENKLIQKFSDSENNSITSHRMDTLKMK 231
K EN+ + + E++ + +D + K
Sbjct: 1156 KKENEEKSETKEIESSKSQKNEVDKKEKK 1184
Score = 30.3 bits (65), Expect = 1.00
Identities = 19/91 (20%), Positives = 41/91 (45%), Gaps = 1/91 (1%)
Frame = -2
Query: 497 KSNKKVLKKAKISNKENTYKNTKSDAALKNE-NKNSINKSLNTKANLQNCSIEKDNFTSE 321
K KK K + + KE K ++ K E NK KS N+K +N ++ + +
Sbjct: 945 KKKKKESKNSNMKKKEEDKKEYVNNELKKQEDNKKETTKSENSKLKEENKDNKEKKESED 1004
Query: 320 PKCENKLIQKFSDSENNSITSHRMDTLKMKD 228
+N+ +++ + ++ + + + K +D
Sbjct: 1005 SASKNREKKEYEEKKSKTKEEAKKEKKKSQD 1035
Score = 27.9 bits (59), Expect = 5.3
Identities = 22/95 (23%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Frame = -2
Query: 494 SNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKA-NLQNCSIEKDNFTSEP 318
+N + K S + YK + K ENK++IN S K + + E N +
Sbjct: 899 ANNMDIDVQKGSGESVKYKKDEKKEGNKEENKDTINTSSKQKGKDKKKKKKESKNSNMKK 958
Query: 317 KCENKLIQKFSDSENNSITSHRMDTLKMKD*QIKK 213
K E+K +++ ++E ++ +T K ++ ++K+
Sbjct: 959 KEEDK--KEYVNNELKKQEDNKKETTKSENSKLKE 991
>At1g17140.2 68414.m02089 tropomyosin-related similar to Tropomyosin
1. [Baker's yeast](SP:P17536) {Saccharomyces cerevisiae}
Length = 344
Score = 29.5 bits (63), Expect = 1.7
Identities = 15/51 (29%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
Frame = -2
Query: 512 ERLLIKSNKKVLKKAKISNKENTYKNT-KSDAALKNENKNSINKSLNTKAN 363
E+L+ K ++ + KA++ + E +++ K + +LKN+ +S ++ N KAN
Sbjct: 163 EKLVAKEDEIKMLKARLYDMEKEHESLGKENESLKNQLSDSASEISNVKAN 213
>At1g17140.1 68414.m02088 tropomyosin-related similar to Tropomyosin
1. [Baker's yeast](SP:P17536) {Saccharomyces cerevisiae}
Length = 344
Score = 29.5 bits (63), Expect = 1.7
Identities = 15/51 (29%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
Frame = -2
Query: 512 ERLLIKSNKKVLKKAKISNKENTYKNT-KSDAALKNENKNSINKSLNTKAN 363
E+L+ K ++ + KA++ + E +++ K + +LKN+ +S ++ N KAN
Sbjct: 163 EKLVAKEDEIKMLKARLYDMEKEHESLGKENESLKNQLSDSASEISNVKAN 213
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 29.1 bits (62), Expect = 2.3
Identities = 22/89 (24%), Positives = 36/89 (40%)
Frame = -2
Query: 497 KSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEP 318
K KK K + KN K+D K+E+ + + L + +N EKD +E
Sbjct: 130 KEGKKKKNKKEKDESGPEEKNKKADKEKKHEDVSQEKEELEEEDGKKNKKKEKDESGTEE 189
Query: 317 KCENKLIQKFSDSENNSITSHRMDTLKMK 231
K + +K E+ S ++ K K
Sbjct: 190 KKKKPKKEKKQKEESKSNEDKKVKGKKEK 218
>At5g64530.1 68418.m08110 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
similar to NAM (no apical meristem)
Length = 187
Score = 28.7 bits (61), Expect = 3.0
Identities = 15/66 (22%), Positives = 33/66 (50%)
Frame = -2
Query: 461 SNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEPKCENKLIQKFSD 282
S+ ++ + +S A + +K +K + + QNCS E+D+ +E C +++ D
Sbjct: 121 SSSSSSRSSKRSSRASSSSHKPDYSKWVICRVYEQNCSEEEDDDGTELSCLDEVFLSLDD 180
Query: 281 SENNSI 264
+ S+
Sbjct: 181 LDEVSL 186
>At3g52770.1 68416.m05814 expressed protein emm32, Streptococcus
pyogenes, EMBL:SPEMM32G
Length = 67
Score = 28.3 bits (60), Expect = 4.0
Identities = 15/53 (28%), Positives = 32/53 (60%), Gaps = 3/53 (5%)
Frame = -2
Query: 503 LIKSNKKVLKKAKISNKEN---TYKNTKSDAALKNENKNSINKSLNTKANLQN 354
++K N+++ KKA++ N+EN ++ + + KN N S N + ++ A+ Q+
Sbjct: 15 IMKENERLRKKAELLNQENQQLLFQLKQKLSKTKNSNNGSNNDNKSSSASGQS 67
>At5g55300.1 68418.m06891 DNA topoisomerase I identical to
Swiss-Prot:P30181 DNA topoisomerase I [Arabidopsis
thaliana]
Length = 916
Score = 27.9 bits (59), Expect = 5.3
Identities = 21/88 (23%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = -2
Query: 494 SNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEPK 315
+++ LKKAKIS + K + + ++K + S KA + S +D +
Sbjct: 234 ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKA--KQLSTREDGTDDDDD 291
Query: 314 CENKLIQKF-SDSENNSITSHRMDTLKM 234
+ + ++F SDS N++ +S + +K+
Sbjct: 292 DDVPISKRFKSDSSNSNTSSAKPKAVKL 319
>At4g33200.1 68417.m04727 myosin, putative similar to myosin
(GI:433663) [Arabidopsis thaliana]
Length = 1522
Score = 27.9 bits (59), Expect = 5.3
Identities = 17/72 (23%), Positives = 35/72 (48%)
Frame = -2
Query: 491 NKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEPKC 312
N + K+ IS KE + + + ++ + N++ K+ +N +EK+ ++ C
Sbjct: 956 NAVLEKQLDISMKEKSAVERELNGMVELKKDNALLKNSMNSLEKKNRVLEKELLNAKTNC 1015
Query: 311 ENKLIQKFSDSE 276
N L QK ++E
Sbjct: 1016 NNTL-QKLKEAE 1026
>At1g77580.2 68414.m09032 myosin heavy chain-related low similarity
to SP|P08799 Myosin II heavy chain, non muscle
{Dictyostelium discoideum}
Length = 779
Score = 27.9 bits (59), Expect = 5.3
Identities = 21/85 (24%), Positives = 37/85 (43%)
Frame = -2
Query: 500 IKSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSE 321
+++ K L+ N+E ++ A + E K L K L+ +EKD SE
Sbjct: 451 LEAEKVELESEVKCNREEAVAQVENSLATEIEVLTCRIKQLEEK--LEKLEVEKDELKSE 508
Query: 320 PKCENKLIQKFSDSENNSITSHRMD 246
KC N+ ++ E +I +M+
Sbjct: 509 VKC-NREVESTLRFELEAIACEKME 532
>At1g77580.1 68414.m09033 myosin heavy chain-related low similarity
to SP|P08799 Myosin II heavy chain, non muscle
{Dictyostelium discoideum}
Length = 629
Score = 27.9 bits (59), Expect = 5.3
Identities = 21/85 (24%), Positives = 37/85 (43%)
Frame = -2
Query: 500 IKSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSE 321
+++ K L+ N+E ++ A + E K L K L+ +EKD SE
Sbjct: 417 LEAEKVELESEVKCNREEAVAQVENSLATEIEVLTCRIKQLEEK--LEKLEVEKDELKSE 474
Query: 320 PKCENKLIQKFSDSENNSITSHRMD 246
KC N+ ++ E +I +M+
Sbjct: 475 VKC-NREVESTLRFELEAIACEKME 498
>At1g65660.1 68414.m07450 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 535
Score = 27.9 bits (59), Expect = 5.3
Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 3/86 (3%)
Frame = -2
Query: 479 LKKAKISNKENTYKNTKSDAALKNENKNSI---NKSLNTKANLQNCSIEKDNFTSEPKCE 309
L KA I+ KE T ++ K + + + + LN +A L N ++D E K E
Sbjct: 441 LMKANIARKEATEESPKKVEEKRMASWGTDIPEDLELNEEA-LANALKKEDLSRREEKDE 499
Query: 308 NKLIQKFSDSENNSITSHRMDTLKMK 231
K +K++ NN +T M+ +MK
Sbjct: 500 RK--RKYNVKYNNDVTPEEMEAYRMK 523
>At5g25870.1 68418.m03069 hypothetical protein
Length = 173
Score = 27.5 bits (58), Expect = 7.0
Identities = 19/71 (26%), Positives = 33/71 (46%)
Frame = -2
Query: 515 MERLLIKSNKKVLKKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKD 336
+ +L+K KK + ++ N+EN K+ LK + K + KS K L++ I+
Sbjct: 77 ISEILVKLEKKYYELEEVENRENLQMKEKT---LKRKYKRELKKSSKIK-ELEDRMIKAG 132
Query: 335 NFTSEPKCENK 303
E + E K
Sbjct: 133 QINDEHERELK 143
>At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase family
protein belongs to Pfam:PF03372:
Endonuclease/Exonuclease/phosphatase family; contains 3
WD-40 repeats (PF00400);similar to Type II
inositol-1,4,5-trisphosphate 5-phosphatase EC 3.1.3.56
(5PTASE) (INPP5B) (SP:P32019) [Homo sapiens]
Length = 1305
Score = 27.5 bits (58), Expect = 7.0
Identities = 20/75 (26%), Positives = 35/75 (46%)
Frame = -2
Query: 470 AKISNKENTYKNTKSDAALKNENKNSINKSLNTKANLQNCSIEKDNFTSEPKCENKLIQK 291
AK ++ N+KS + KNE ++ S + + + S +K + S K K
Sbjct: 1096 AKNLRIDSNPSNSKSQSLKKNEGDSNSKSSKKSDGDSNSKSSKKSDGDSNSKSSKK---- 1151
Query: 290 FSDSENNSITSHRMD 246
SD ++NS +S + D
Sbjct: 1152 -SDGDSNSKSSKKSD 1165
>At5g28290.1 68418.m03434 protein kinase, putative similar to
LSTK-1-like kinase [Lycopersicon esculentum]
GI:15637110; contains Pfam profile: PF00069 Eukaryotic
protein kinase domain
Length = 568
Score = 27.1 bits (57), Expect = 9.3
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 356 NCSIEKDNFTSEPKCENK 303
+CSI KD FT EP+ E K
Sbjct: 458 DCSITKDKFTLEPERETK 475
>At4g37120.1 68417.m05257 expressed protein
Length = 536
Score = 27.1 bits (57), Expect = 9.3
Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 3/86 (3%)
Frame = -2
Query: 479 LKKAKISNKENTYKNTKSDAALKNENKNSI---NKSLNTKANLQNCSIEKDNFTSEPKCE 309
L KA I+ KE + ++ K K + + LN +A L N ++D E K E
Sbjct: 441 LMKANIARKEASKESPKKVEEKKMATWGTDIPEDLELNEEA-LANALKKEDLSRREEKDE 499
Query: 308 NKLIQKFSDSENNSITSHRMDTLKMK 231
K +K++ + N +TS M+ +MK
Sbjct: 500 RK--RKYNVNYTNDVTSEEMEAYRMK 523
>At1g33390.1 68414.m04133 helicase domain-containing protein similar
to kurz protein [Drosophila melanogaster] GI:5869803;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1237
Score = 27.1 bits (57), Expect = 9.3
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 476 KKAKISNKENTYKNTKSDAALKNENKNSINKSLNTKA 366
KK K SNK N+ ++ K K +N ++NT A
Sbjct: 19 KKNKGSNKMQDKLNSNNNTGSKKSRKRKLNSNVNTVA 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,129,903
Number of Sequences: 28952
Number of extensions: 140650
Number of successful extensions: 476
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 459
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 475
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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