Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV1016.Seq
         (399 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    29   0.20 
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb...    29   0.35 
SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyce...    25   3.3  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    25   4.3  
SPAC25H1.08c |||ribosome biogenesis protein Sqt1|Schizosaccharom...    25   5.7  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    24   7.6  
SPCPJ732.03 |meu15||sequence orphan|Schizosaccharomyces pombe|ch...    24   10.0 

>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 29.5 bits (63), Expect = 0.20
 Identities = 23/70 (32%), Positives = 26/70 (37%), Gaps = 7/70 (10%)
 Frame = +3

Query: 48  VYHPPAVPVTLIQTQTARDPVHPVV-----AALMDTVPTV--VMEVYHPPAVPVTLMQAQ 206
           V+ PPA PV        + P  PVV           VP V   + V  PP  PV      
Sbjct: 529 VHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPS 588

Query: 207 TLQDPVHPAV 236
             Q PV P V
Sbjct: 589 VPQPPVAPVV 598



 Score = 25.8 bits (54), Expect = 2.5
 Identities = 26/79 (32%), Positives = 29/79 (36%), Gaps = 9/79 (11%)
 Frame = +3

Query: 27  VPTV--VMEVYHPPAVPVTLIQTQTARDPVHPVV--AALMDTVPTV-----VMEVYHPPA 179
           VP V   + V  PP  PV        + PV PVV  A  +   P       V  V   PA
Sbjct: 565 VPVVPEALSVPQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEVPSVPQRPA 624

Query: 180 VPVTLMQAQTLQDPVHPAV 236
           VPV        Q P  P V
Sbjct: 625 VPVVPEAPSVPQPPAAPVV 643


>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 955

 Score = 28.7 bits (61), Expect = 0.35
 Identities = 21/89 (23%), Positives = 39/89 (43%), Gaps = 5/89 (5%)
 Frame = +3

Query: 6   VAALMDTVPTV-VMEVYHPPAVPV--TLIQTQTARDPVHPVVAALMDTVP--TVVMEVYH 170
           +A ++D  P   V +  H    P   +++  Q   +P    + AL D +   T+ + + H
Sbjct: 576 LAHIVDIQPVANVYQSVHSFLAPSLQSILLAQVKLNPTQAELEALADNIDIVTIFLSLVH 635

Query: 171 PPAVPVTLMQAQTLQDPVHPAVAALMDTF 257
           PP+    L     L   + P ++  +DTF
Sbjct: 636 PPSPAGELHPIVRLFQDIWPILSRTLDTF 664


>SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 363

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 9/16 (56%), Positives = 10/16 (62%)
 Frame = -3

Query: 247 ISAATAGCTGSCKVCA 200
           +S  T GCT  CK CA
Sbjct: 62  LSIKTGGCTEDCKYCA 77



 Score = 25.0 bits (52), Expect = 4.3
 Identities = 9/25 (36%), Positives = 12/25 (48%)
 Frame = -1

Query: 387 PSPQSEAXSISAATAGCTGSCQVCA 313
           P    +   +S  T GCT  C+ CA
Sbjct: 53  PKKVQQCTLLSIKTGGCTEDCKYCA 77


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 11/35 (31%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
 Frame = -2

Query: 320 SVLASVLLEL-PVDDTLPSRLSECIHKCCYRRMYW 219
           S+L   LL L    ++L S+   C+  CC    +W
Sbjct: 248 SILKDYLLNLVKTSESLSSQQMSCLRICCNSSSFW 282


>SPAC25H1.08c |||ribosome biogenesis protein
           Sqt1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 399

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = -1

Query: 390 LPSPQSEAXSISAATAGCTGSCQVCACISVTGT 292
           L S +++  S+ A  A CT +  +CAC SV GT
Sbjct: 278 LASLEAQTESVEAI-ALCT-ALPICACASVDGT 308


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 24.2 bits (50), Expect = 7.6
 Identities = 20/77 (25%), Positives = 31/77 (40%), Gaps = 5/77 (6%)
 Frame = +3

Query: 12   ALMDTVPTVVMEVYHPPAVP---VTLIQTQTARDPVHPVV-AALMDTVPTVVMEVYHPPA 179
            A+    P  V E YHPP      V  +Q  +  +P  PV   + +     +      PP+
Sbjct: 922  AIASPPPPKVGETYHPPTASGTRVPPVQQPSHPNPYTPVAPQSPVAAASRISSSPNMPPS 981

Query: 180  VPVT-LMQAQTLQDPVH 227
             P T +  A +  +P H
Sbjct: 982  NPYTPIAVASSTVNPAH 998


>SPCPJ732.03 |meu15||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 150

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 17/71 (23%), Positives = 30/71 (42%), Gaps = 11/71 (15%)
 Frame = -2

Query: 245 KCCYRRMYWIL*GLCLHQCYWNCRWMIHF-----HHDC---WNCIHKCCYHRMY---WIP 99
           K  Y+  YW L  + L Q +W    ++       H++    W  I +  Y+ +Y   ++ 
Sbjct: 31  KKIYKISYWWLCSIALIQTWWVLLGLLPIFFALEHYEVPYKWIFIIEMLYYELYLTLYVC 90

Query: 98  RCLSLNQCYWN 66
            C  L   +WN
Sbjct: 91  WCYKLRSDFWN 101


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,378,669
Number of Sequences: 5004
Number of extensions: 26193
Number of successful extensions: 84
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)