Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1013.Seq
(449 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 35 0.022
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 31 0.27
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 31 0.36
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 31 0.48
At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) simil... 30 0.83
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 29 1.1
At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase f... 27 4.4
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 35.1 bits (77), Expect = 0.022
Identities = 18/54 (33%), Positives = 31/54 (57%)
Frame = +2
Query: 62 VLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKE 223
+L+ + +G LL +G + +S E+L ++ DAL+VRS T+VT+E
Sbjct: 85 ILVTEKLGEAGVNLLREFGDVDCSY-DLSPEDLKKKVAESDALIVRSGTKVTRE 137
Score = 32.3 bits (70), Expect = 0.16
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +1
Query: 277 DNIDVDSAGKKGVGVINAPGANALSACELTCTVDVGSGXPRGPSFTALKAGR 432
DN+D+ +A + G V+NAP AN ++A E + + ++KAG+
Sbjct: 157 DNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGK 208
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 31.5 bits (68), Expect = 0.27
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +1
Query: 277 DNIDVDSAGKKGVGVINAPGANALSACE 360
DN+D+ +A + G V+NAP AN ++A E
Sbjct: 136 DNVDLSAATEFGCLVVNAPTANTIAAAE 163
Score = 26.6 bits (56), Expect = 7.7
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +2
Query: 143 ISKEELLMEIPNHDALVVRSATQVTKE 223
++ EEL ++I DAL+VRS T+V +E
Sbjct: 90 MTPEELNIKISLCDALIVRSGTKVGRE 116
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 31.1 bits (67), Expect = 0.36
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +1
Query: 277 DNIDVDSAGKKGVGVINAPGANALSACELTCTVDVGSGXPRGPSFTALKAGR 432
DN+D+ +A + G V+NAP AN ++A E + + ++KAG+
Sbjct: 121 DNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGK 172
Score = 30.7 bits (66), Expect = 0.48
Identities = 18/55 (32%), Positives = 31/55 (56%)
Frame = +2
Query: 59 SVLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKE 223
++L+ + +G +LL Y + +S EEL +I DAL+VRS T+V ++
Sbjct: 48 TILVTEKLGQAGIDLLKKYANVDCSY-DLSLEELCTKISLCDALIVRSGTKVGRD 101
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 30.7 bits (66), Expect = 0.48
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +1
Query: 277 DNIDVDSAGKKGVGVINAPGANALSACELTCTVDVGS 387
+N+DV++A K G+ V N PG + EL ++ + +
Sbjct: 98 NNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAA 134
>At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) similar
to glutamate decarboxylase (gad) GI:294111 from [Petunia
hybrida]
Length = 494
Score = 29.9 bits (64), Expect = 0.83
Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +2
Query: 38 KMVVDIKSVLI-VDGVGAKCAELLNAYGIATTTKAKISKEELLMEI 172
++V DI VL +D + +K ++ + GIA K K ++E+LME+
Sbjct: 431 RLVADISKVLHELDTLPSKISKKMGIEGIAENVKEKKMEKEILMEV 476
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 29.5 bits (63), Expect = 1.1
Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = +1
Query: 61 GFDRRRGWRQVCRTPQR-LRNRHHYQGQDLQGRTSYGDTQPRRSGCAFSNSS 213
G R+ RQ +R L N+HH + QD+Q + Y Q G + S SS
Sbjct: 1165 GAGRKSRQRQPDLMKKRMLLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSS 1216
>At5g65450.1 68418.m08231 ubiquitin carboxyl-terminal hydrolase
family protein / zinc finger (MYND type) family protein
similar to ubiquitin-specific protease 16 (UBP16)
[Arabidopsis thaliana] GI:11993477; contains Pfam
profiles PF00443: Ubiquitin carboxyl-terminal hydrolase,
PF01753: MYND finger
Length = 731
Score = 27.5 bits (58), Expect = 4.4
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +1
Query: 73 RRGWRQVCRTPQRLRNRHHYQGQDLQGRTSYGDTQPRRSGCAFSNSSD 216
RRG ++ CR+P + Y D + S D R G A+ +SS+
Sbjct: 87 RRGHKEECRSPDYDEEKEEYVQSDYDAKESNVDFPSR--GTAYESSSN 132
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,795,233
Number of Sequences: 28952
Number of extensions: 118681
Number of successful extensions: 337
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 331
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 337
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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