BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1011.Seq
(409 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 32 0.039
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 31 0.069
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 1.1
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 2.0
SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr 3... 25 3.4
SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3... 25 3.4
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 25 4.5
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.0
SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizo... 24 7.9
SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid transporter|Schizo... 24 7.9
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 24 7.9
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 31.9 bits (69), Expect = 0.039
Identities = 17/39 (43%), Positives = 23/39 (58%)
Frame = +3
Query: 126 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 242
N D KFP K+P F DG L+E+ AIA+Y+A+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 31.1 bits (67), Expect = 0.069
Identities = 25/76 (32%), Positives = 32/76 (42%)
Frame = +3
Query: 6 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 185
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 186 ADGKVLLTESNAIAYY 233
ADG L+E AI Y
Sbjct: 58 ADG-FELSEVIAIVKY 72
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 1.1
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 33 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 212
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 213 SN 218
SN
Sbjct: 612 SN 613
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 2.0
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -1
Query: 202 STFPSALSNAGTFPAGNFFKKSSDL 128
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPCC1183.03c |||frataxin homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 158
Score = 25.4 bits (53), Expect = 3.4
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = +3
Query: 186 ADGKVLLTESNAIAYYVANESLRVEIWLPKPVSGXGH 296
A+G + L Y + + +IWL PVSG H
Sbjct: 79 ANGVITLMLGEKGTYVINKQPPAHQIWLSSPVSGPKH 115
>SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 25.4 bits (53), Expect = 3.4
Identities = 8/27 (29%), Positives = 19/27 (70%)
Frame = -2
Query: 246 FHWQRSKRWHCFQLGAPFHRHFRMQAL 166
FH ++ +HC+++G P ++++ +AL
Sbjct: 363 FHHDYTE-YHCYRIGKPLYQNYEEEAL 388
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 25.0 bits (52), Expect = 4.5
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +3
Query: 9 AAGVLYTYPENFRAYKALIAAQYSG-TDVKVAPNFV-FGE 122
A G +YT E+FRA K+L S T V+ FV FGE
Sbjct: 329 ALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELPFVNFGE 368
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 24.6 bits (51), Expect = 6.0
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +3
Query: 207 TESNAIAYYVANESLRVEIWLPK 275
+ES I YY N E+WLP+
Sbjct: 722 SESRIIVYYSNNTLYLSELWLPQ 744
>SPAC20G4.04c |hus1||checkpoint clamp complex protein
Hus1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 287
Score = 24.2 bits (50), Expect = 7.9
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +1
Query: 70 HNIPGLM*K*HRISYLARPTSPKTS*RSFLPE 165
HNIP + +S + PT+P+ FLP+
Sbjct: 125 HNIPVRVLSQSYVSVIKEPTAPEPDCHIFLPQ 156
>SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 594
Score = 24.2 bits (50), Expect = 7.9
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3
Query: 93 KVAPNFVFGETNKSEDFLK 149
K+ P+ F E + SEDFLK
Sbjct: 52 KLRPDNEFAEVHNSEDFLK 70
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 24.2 bits (50), Expect = 7.9
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = -2
Query: 210 QLGAPFHRHFRMQALFRQETSSRSLRTCWSRQIRNSVLLSHQSRNIVRRSTLYKRGSFPD 31
Q G+P R +M+AL R + S+ + RNSVL + + ++ K G +
Sbjct: 1418 QPGSPSKRSGKMEALIRNFDQNSSIPDPFIVNQRNSVLQTEFEKINLKLKEATKSGILDN 1477
Query: 30 K 28
K
Sbjct: 1478 K 1478
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,613,312
Number of Sequences: 5004
Number of extensions: 30213
Number of successful extensions: 89
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 89
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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