Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV1011.Seq
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 73 6e-14
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 72 2e-13
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 45 2e-05
At4g02520.1 68417.m00345 glutathione S-transferase, putative 45 2e-05
At2g02930.1 68415.m00241 glutathione S-transferase, putative 44 4e-05
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 42 2e-04
At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 40 8e-04
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 38 0.002
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 38 0.003
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 38 0.003
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 38 0.003
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 38 0.003
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 35 0.024
At5g46550.1 68418.m05731 DNA-binding bromodomain-containing prot... 29 0.91
At4g22130.1 68417.m03199 protein kinase family protein contains ... 29 1.6
At1g59820.1 68414.m06735 haloacid dehalogenase-like hydrolase fa... 27 4.8
At5g24090.1 68418.m02829 acidic endochitinase (CHIB1) identical ... 27 6.4
At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative / phos... 27 6.4
At4g29280.1 68417.m04186 expressed protein ; expression supporte... 26 8.5
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 73.3 bits (172), Expect = 6e-14
Identities = 37/77 (48%), Positives = 52/77 (67%)
Frame = +3
Query: 18 VLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
V++TY N A KALIAA+Y+G ++ + +F G TNKS +FLK P GKVP E+ +G
Sbjct: 4 VMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGP 63
Query: 198 VLLTESNAIAYYVANES 248
+ ESNAIA YV+ ++
Sbjct: 64 IF--ESNAIARYVSRKN 78
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 71.7 bits (168), Expect = 2e-13
Identities = 39/74 (52%), Positives = 47/74 (63%)
Frame = +3
Query: 18 VLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
VL+TY N A KALIAA+Y G + V +F G TNK+ FLK P GKVP E+ +G
Sbjct: 4 VLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGS 63
Query: 198 VLLTESNAIAYYVA 239
V ESNAIA YV+
Sbjct: 64 VF--ESNAIARYVS 75
>At1g02930.1 68414.m00260 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 208
Score = 45.2 bits (102), Expect = 2e-05
Identities = 27/80 (33%), Positives = 40/80 (50%)
Frame = +3
Query: 6 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 185
MA ++ +P + + LIA D + + +K E F+ + P GKVPAFE
Sbjct: 1 MAGIKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFED 60
Query: 186 ADGKVLLTESNAIAYYVANE 245
D K+ ES AI Y+A+E
Sbjct: 61 GDFKIF--ESRAITQYIAHE 78
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 44.8 bits (101), Expect = 2e-05
Identities = 27/79 (34%), Positives = 41/79 (51%)
Frame = +3
Query: 6 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 185
MA ++ +P + + LIA D ++ + +K E FL + P G+VPAFE
Sbjct: 1 MAGIKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE- 59
Query: 186 ADGKVLLTESNAIAYYVAN 242
DG + L ES AI Y+A+
Sbjct: 60 -DGDLKLFESRAITQYIAH 77
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 44.0 bits (99), Expect = 4e-05
Identities = 27/79 (34%), Positives = 41/79 (51%)
Frame = +3
Query: 6 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 185
MA ++ +P + + LIA D ++ + +K E FL + P G+VPAFE
Sbjct: 1 MAGIKVFGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE- 59
Query: 186 ADGKVLLTESNAIAYYVAN 242
DG + L ES AI Y+A+
Sbjct: 60 -DGDLKLFESRAITQYIAH 77
>At1g02920.1 68414.m00259 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]; supported by cDNA GI:443697.
Length = 209
Score = 41.9 bits (94), Expect = 2e-04
Identities = 27/79 (34%), Positives = 39/79 (49%)
Frame = +3
Query: 6 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 185
MA ++ +P + + LIA D + + +K E F+ + P GKVPAFE
Sbjct: 1 MAGIKVFGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFE- 59
Query: 186 ADGKVLLTESNAIAYYVAN 242
DG L ES AI Y+A+
Sbjct: 60 -DGDFKLFESRAITQYIAH 77
>At2g02380.1 68415.m00176 glutathione S-transferase, putative
similar to gi:167970 gb:AAA72320 gb:AY052332
Length = 223
Score = 39.5 bits (88), Expect = 8e-04
Identities = 27/73 (36%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +3
Query: 21 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
LY+Y + A++ IA G D + P N + G+ + S DF K P G VPA DG
Sbjct: 14 LYSYWRSSCAHRVRIALTLKGLDYEYIPVNLLKGDQSDS-DFKKINPMGTVPAL--VDGD 70
Query: 198 VLLTESNAIAYYV 236
V++ +S AI Y+
Sbjct: 71 VVINDSFAIIMYL 83
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 38.3 bits (85), Expect = 0.002
Identities = 25/74 (33%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Frame = +3
Query: 21 LYTYPENFRAYKALIAAQYSGTDVK-VAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
+Y YP + + L G + N + G+ K FL P G+VP F DG
Sbjct: 41 IYGYPYSTNTRRVLAVLHEKGLSYDPITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGG 97
Query: 198 VLLTESNAIAYYVA 239
+ LTES AI+ Y+A
Sbjct: 98 LKLTESRAISEYIA 111
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 37.9 bits (84), Expect = 0.003
Identities = 27/73 (36%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +3
Query: 21 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
LY+Y + A++ IA G D + P N + G+ S DF K P G VPA DG
Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGD 67
Query: 198 VLLTESNAIAYYV 236
V++ +S AI Y+
Sbjct: 68 VVINDSFAIIMYL 80
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 37.9 bits (84), Expect = 0.003
Identities = 27/73 (36%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +3
Query: 21 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 197
LY+Y + A++ IA G D + P N + G+ S DF K P G VPA DG
Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGD 67
Query: 198 VLLTESNAIAYYV 236
V++ +S AI Y+
Sbjct: 68 VVINDSFAIIMYL 80
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 37.5 bits (83), Expect = 0.003
Identities = 20/38 (52%), Positives = 24/38 (63%)
Frame = +3
Query: 126 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA 239
+K+E FL P G+VP FE DG V L ES AI Y+A
Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIA 99
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 37.5 bits (83), Expect = 0.003
Identities = 20/38 (52%), Positives = 24/38 (63%)
Frame = +3
Query: 126 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVA 239
+K+E FL P G+VP FE DG V L ES AI Y+A
Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIA 97
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 34.7 bits (76), Expect = 0.024
Identities = 27/79 (34%), Positives = 39/79 (49%), Gaps = 7/79 (8%)
Frame = +3
Query: 21 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKS------EDFLKKFPAGKVPAF 179
LY+Y + A++ IA G D + P N + G+ S +DF K P G VPA
Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL 70
Query: 180 ESADGKVLLTESNAIAYYV 236
DG V++ +S AI Y+
Sbjct: 71 --VDGDVVINDSFAIIMYL 87
>At5g46550.1 68418.m05731 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 494
Score = 29.5 bits (63), Expect = 0.91
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +3
Query: 57 ALIAAQYSGTDVKVAPNFVFGETNKSE 137
A++ AQY+GT +K V G+ NK++
Sbjct: 342 AILKAQYAGTIIKAKHRIVLGQNNKAD 368
>At4g22130.1 68417.m03199 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 338
Score = 28.7 bits (61), Expect = 1.6
Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%)
Frame = +3
Query: 45 RAYKALIAAQYSGTDVKVAPNF-----VFGETNKSEDFLKKFPAGKVPAFESADGKVL-L 206
R + A+QY+ + ++VA N + GE + + +FP GK+ A + D L L
Sbjct: 8 RIRSPITASQYTVSSLQVATNSFSQENIIGEGSLGRVYRAEFPNGKIMAIKKIDNAALSL 67
Query: 207 TESNAIAYYVANES 248
E + V+N S
Sbjct: 68 QEEDNFLEAVSNMS 81
>At1g59820.1 68414.m06735 haloacid dehalogenase-like hydrolase
family protein similar to Potential
phospholipid-transporting ATPase (EC 3.6.3.1) from Mus
musculus [SP|P70704], {Bos taurus} SP|Q29449; contains
InterPro accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 1213
Score = 27.1 bits (57), Expect = 4.8
Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 4/50 (8%)
Frame = +2
Query: 191 WKGAPN*KQCHRLLR----CQ*KSPXGDLATQARVWQXASWSDSELLXAS 328
W+ PN C L R C P GD + + V+Q AS ++ L+ A+
Sbjct: 481 WRNEPNPDLCKELFRCLAICHTVLPEGDESPEKIVYQAASPDEAALVTAA 530
>At5g24090.1 68418.m02829 acidic endochitinase (CHIB1) identical to
SP|P19172 Acidic endochitinase precursor (EC 3.2.1.14)
{Arabidopsis thaliana}
Length = 302
Score = 26.6 bits (56), Expect = 6.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -1
Query: 301 PXCPLPDTGLGSQISTRR 248
P CP PD +GS ++T+R
Sbjct: 186 PQCPFPDRLMGSALNTKR 203
>At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar to SEC14
CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/
PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) GB:P46250 from
[Candida albicans] (Yeast (1996) 12(11), 1097-1105);
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 579
Score = 26.6 bits (56), Expect = 6.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -2
Query: 159 QETSSRSLRTCWSRQIRN 106
+E+SS +R+CW R RN
Sbjct: 557 KESSSTGMRSCWPRHCRN 574
>At4g29280.1 68417.m04186 expressed protein ; expression supported
by MPSS
Length = 77
Score = 26.2 bits (55), Expect = 8.5
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = -3
Query: 176 CRHFSGRKLLQEVFGLVGLAKYEIRCYFHISPGILC 69
C F L+ VF LV AK + RC I PG C
Sbjct: 6 CSSFFVLMLVFSVFSLVEKAKGDERCTIIIHPGSPC 41
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,659,170
Number of Sequences: 28952
Number of extensions: 165715
Number of successful extensions: 421
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 421
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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