Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0994.Seq
(553 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53800.1 68418.m06685 expressed protein 32 0.29
At5g55920.1 68418.m06975 nucleolar protein, putative similar to ... 31 0.39
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 30 0.89
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 30 0.89
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 29 1.6
At1g56660.1 68414.m06516 expressed protein 29 1.6
At1g31460.1 68414.m03852 expressed protein 29 1.6
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 29 2.1
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 29 2.1
At1g10890.1 68414.m01251 F-box family protein contains Pfam PF00... 29 2.7
At2g16140.1 68415.m01850 expressed protein contains similarity t... 28 3.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 28 3.6
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 28 3.6
At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related simil... 28 3.6
At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase, p... 28 4.8
At1g62970.1 68414.m07110 DNAJ heat shock N-terminal domain-conta... 28 4.8
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 28 4.8
At1g65190.1 68414.m07391 protein kinase family protein contains ... 27 6.3
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 27 8.3
At3g25840.1 68416.m03219 protein kinase family protein contains ... 27 8.3
At1g11420.1 68414.m01312 agenet domain-containing protein contai... 27 8.3
>At5g53800.1 68418.m06685 expressed protein
Length = 351
Score = 31.9 bits (69), Expect = 0.29
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -3
Query: 233 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 99
+R +RE +EK+ K R+ K KR +D ++ KE+ K +K
Sbjct: 136 KRKEREEEEKERKRRRREKDKKKRNKSDKDGDKKRKEKKKKKSEK 180
>At5g55920.1 68418.m06975 nucleolar protein, putative similar to
SP|P46087 Proliferating-cell nucleolar antigen p120
(Proliferation-associated nucleolar protein p120) {Homo
sapiens}, SP|P40991 Nucleolar protein NOP2
{Saccharomyces cerevisiae}; contains Pfam profile
PF01189: NOL1/NOP2/sun family
Length = 682
Score = 31.5 bits (68), Expect = 0.39
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 7/52 (13%)
Frame = -3
Query: 239 PSERNQREVKEK--KSKANMR--KTTKGKRTTADPPNI---QREKERVWKNQ 105
P ER +++ KEK KSK + R K K K + P+ Q++K R WKN+
Sbjct: 611 PKERKEKKNKEKLAKSKEDKRGKKDKKSKSENVEEPSKPRKQKKKRREWKNE 662
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 30.3 bits (65), Expect = 0.89
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = -3
Query: 233 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 102
E+ +E KEKK K K K K ++EKER K +K
Sbjct: 79 EKKDKEEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERK 122
Score = 29.1 bits (62), Expect = 2.1
Identities = 17/60 (28%), Positives = 28/60 (46%)
Frame = -3
Query: 233 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 54
E +++ KE+K K K K K+ ++EKER K +K S + R ++P
Sbjct: 84 EEKEKKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEKKDKEESEAAARYRILSP 143
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 30.3 bits (65), Expect = 0.89
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -3
Query: 221 REVKEKKSKANMRKTTKGKRTTADPPNIQREK-ERVWKNQKKGTASSRLSPSR 66
REV +K+ ++ T K +RT D R + ER + SSR SP R
Sbjct: 65 REVGDKRRRSGREDTEKRRRTRTDDERYSRGRHERSTSPSDRSHRSSRRSPER 117
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -3
Query: 233 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKG 96
++ +RE KEKKSK + K ++ D Q++KE K ++KG
Sbjct: 23 DKEKREGKEKKSKDRSKDKQKERKEKKDKHKDQKDKE---KGKEKG 65
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 29.5 bits (63), Expect = 1.6
Identities = 27/126 (21%), Positives = 47/126 (37%)
Frame = -3
Query: 500 DLKDELTEDXXXXXXXXKDEGLTLXKEGYKSDYDRNEYEERGSEHQEDTDSDXXXXXXXX 321
D+K E E K E L KEG K + + E E + D +
Sbjct: 105 DVKVEEHEKEHKKGKEKKHEELEEEKEGKKKKNKKEKDESGPEEKNKKADKEKKHEDVSQ 164
Query: 320 XXXXXXXXXXXERRTHDKFSIGRTVLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPN 141
+ + +K G + +++ KE+ SK+N K KGK+ + +
Sbjct: 165 EKEELEEEDGKKNKKKEKDESGTEEKKKKPKKEKKQKEE-SKSNEDKKVKGKKEKGEKGD 223
Query: 140 IQREKE 123
+++E E
Sbjct: 224 LEKEDE 229
>At1g31460.1 68414.m03852 expressed protein
Length = 301
Score = 29.5 bits (63), Expect = 1.6
Identities = 16/69 (23%), Positives = 33/69 (47%)
Frame = -3
Query: 248 VLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPS 69
+L P ++ ++ K+ + + ++ A PP+ E E+ K + K + +LSPS
Sbjct: 149 ILSPVKQQTKKKLPKEKRRIVSPSSSSSIDLATPPSTDSEPEKKSKPKSKSSWFDKLSPS 208
Query: 68 RWVNPA*RS 42
+ + RS
Sbjct: 209 KLIGSIWRS 217
>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 2.1
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 239 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 123
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 29.1 bits (62), Expect = 2.1
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 239 PSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKE 123
PSE+ ++ VKEKK K K K K A Q++K+
Sbjct: 4 PSEQKEKVVKEKKEKVKKEKVVKEKVAKASSSG-QKKKD 41
>At1g10890.1 68414.m01251 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 592
Score = 28.7 bits (61), Expect = 2.7
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -3
Query: 236 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRL 78
+E N + V+E + K M + K + + +QR+KE + +K RL
Sbjct: 159 AEENLKRVEEAQRKEAMERQRKEEERYRELEELQRQKEEAMRRKKAEEEEERL 211
>At2g16140.1 68415.m01850 expressed protein contains similarity to
hypothetical proteins
Length = 311
Score = 28.3 bits (60), Expect = 3.6
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 4/66 (6%)
Frame = -3
Query: 284 RRTHDKFSIGRTVLVPS----ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERV 117
RR DK + T PS E R + K +KA +KT T D N E + +
Sbjct: 190 RRKCDKAAQPSTSQPPSHGVEEAMSRPIGVKAAKAKAKKTVTKTTTVEDKGNAMLEIQSI 249
Query: 116 WKNQKK 99
W+ ++K
Sbjct: 250 WEIKQK 255
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 28.3 bits (60), Expect = 3.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 236 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 105
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 455 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 497
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 28.3 bits (60), Expect = 3.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -3
Query: 236 SERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQ 105
+E +R+ KEK+ + +RK +GK TA + +K +KNQ
Sbjct: 408 AEEAKRKRKEKEKEKLLRKKLEGKLLTA-KQKTEAQKREAFKNQ 450
>At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related similar
to U2 small nuclear ribonucleoprotein auxiliary factor
35 kD subunit related protein 1 (sp|Q15695)
Length = 757
Score = 28.3 bits (60), Expect = 3.6
Identities = 19/92 (20%), Positives = 37/92 (40%)
Frame = -3
Query: 281 RTHDKFSIGRTVLVPSERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQK 102
R HD ++G T S + + ++ + +R+ + D P+ ER ++
Sbjct: 469 RDHDHANVGSTPSYRSRKYHGDTQDSTREDKLRRHAENCHDGDDSPSRDGSLEREMYKER 528
Query: 101 KGTASSRLSPSRWVNPA*RSRIIQNRIPTRKS 6
+ + SRW + R+ + RI R S
Sbjct: 529 RYAKDTLHRDSRWSEHSPGHRVGRKRIHGRYS 560
>At2g30840.1 68415.m03760 2-oxoglutarate-dependent dioxygenase,
putative similar to 2A6 (GI:599622) and tomato ethylene
synthesis regulatory protein E8 (SP|P10967)
Length = 362
Score = 27.9 bits (59), Expect = 4.8
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +2
Query: 2 FDSSLSEYDFELSCFFKPDLPNEKDLDD 85
+ S + + LSCF PD+P +DL D
Sbjct: 142 YSSPSANWRDTLSCFMAPDVPETEDLPD 169
>At1g62970.1 68414.m07110 DNAJ heat shock N-terminal
domain-containing protein low similarity to AHM1
[Triticum aestivum] GI:6691467; contains Pfam profile
PF00226: DnaJ domain
Length = 797
Score = 27.9 bits (59), Expect = 4.8
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 260 WRTCRGSYVLHTFPLHRIFRIRNLCCMSRRCR 355
W TC YVL+ +P+ I+ L C ++ CR
Sbjct: 646 WTTCPYCYVLYEYPI--IYEESVLKCQTKSCR 675
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 27.9 bits (59), Expect = 4.8
Identities = 20/53 (37%), Positives = 24/53 (45%)
Frame = -3
Query: 212 KEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKKGTASSRLSPSRWVNP 54
KEK+ KA KTTK +R +E +R K KG S R S NP
Sbjct: 284 KEKEPKALKDKTTKAERRAI------QEAQRAAKAAAKGEGSRRADESGRANP 330
>At1g65190.1 68414.m07391 protein kinase family protein contains
similarity to protein kinases
Length = 396
Score = 27.5 bits (58), Expect = 6.3
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3
Query: 440 GLTLXKEGYKSDYDRNEYEERGSEHQEDTDSD 345
GLTL GYKS ++ EE+ SE ++ D+D
Sbjct: 238 GLTLLL-GYKSYFEHYRGEEKESEEEDPEDTD 268
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = -3
Query: 233 ERNQREVKEKKSKANMRKTTKGKRTTADPPNIQREKERVWKNQKK 99
E Q+E E K RK ++ K+ + Q+E K ++K
Sbjct: 747 EETQKEANESTKKERKRKKSESKKQSDGEEETQKEPSESTKKERK 791
>At3g25840.1 68416.m03219 protein kinase family protein contains
Pfam profile: PF00069 eukaryotic protein kinase domain
Length = 935
Score = 27.1 bits (57), Expect = 8.3
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = -2
Query: 255 QNGISAERTKSKRGERK-EVEGEYEKDYER 169
+ G S +RT+ ERK E E E EKD+ER
Sbjct: 333 ERGRSRDRTRYNSRERKREKEREGEKDWER 362
>At1g11420.1 68414.m01312 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 604
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -2
Query: 279 DPRQVLHWQNGISAERTKSKRGERKEVEG 193
D R + W++G+ +RTK KR + ++G
Sbjct: 262 DIRPPMEWEDGVWHKRTKPKRQKETSLDG 290
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,935,511
Number of Sequences: 28952
Number of extensions: 124814
Number of successful extensions: 581
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 575
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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